##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146644_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3754843 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.449507476078228 31.0 31.0 34.0 28.0 34.0 2 31.595092790830403 33.0 31.0 34.0 28.0 34.0 3 31.711444393280892 33.0 31.0 34.0 28.0 34.0 4 35.14571927508021 37.0 35.0 37.0 32.0 37.0 5 35.292008214457965 37.0 35.0 37.0 32.0 37.0 6 35.386627350331295 37.0 35.0 37.0 32.0 37.0 7 35.331522250064786 37.0 35.0 37.0 32.0 37.0 8 35.29852459876485 37.0 35.0 37.0 32.0 37.0 9 36.83101956593125 39.0 37.0 39.0 32.0 39.0 10-11 36.67745708675436 39.0 37.0 39.0 32.0 39.0 12-13 36.6078974540347 39.0 37.0 39.0 32.0 39.0 14-15 37.800760377997165 40.0 38.0 41.0 32.0 41.0 16-17 37.82596555967853 40.0 38.0 41.0 32.0 41.0 18-19 37.793801365330054 40.0 38.0 41.0 32.0 41.0 20-21 37.851972106423624 40.0 38.0 41.0 32.0 41.0 22-23 37.77846250828597 40.0 37.5 41.0 32.0 41.0 24-25 37.72426876436644 40.0 37.0 41.0 31.5 41.0 26-27 37.75108266843647 40.0 37.0 41.0 32.0 41.0 28-29 37.74044693746183 40.0 37.0 41.0 32.0 41.0 30-31 37.609087383946544 40.0 37.0 41.0 31.0 41.0 32-33 37.60198868501293 40.0 37.0 41.0 31.0 41.0 34-35 37.53827217276461 40.0 37.0 41.0 31.0 41.0 36-37 37.41977813719508 40.0 36.5 41.0 31.0 41.0 38-39 37.31346610231107 40.0 36.0 41.0 30.0 41.0 40-41 37.2241650316671 40.0 36.0 41.0 30.0 41.0 42-43 37.05853746747867 40.0 36.0 41.0 30.0 41.0 44-45 36.89764032743846 39.0 35.0 41.0 30.0 41.0 46-47 36.88506683235491 39.0 35.0 41.0 30.0 41.0 48-49 36.733540656693236 39.0 35.0 41.0 30.0 41.0 50-51 36.53831931188601 39.0 35.0 41.0 29.5 41.0 52-53 36.307465984596426 39.0 35.0 41.0 29.0 41.0 54-55 36.07749977828634 38.0 35.0 40.0 29.0 41.0 56-57 35.81751926778297 38.0 34.5 40.0 28.5 41.0 58-59 35.492208861995024 37.0 34.0 40.0 28.0 41.0 60-61 35.23092683236023 36.5 34.0 40.0 28.0 41.0 62-63 34.902639204888196 36.0 34.0 39.5 28.0 41.0 64-65 34.730951999857254 35.5 34.0 39.0 28.0 41.0 66-67 34.465306006136615 35.0 33.0 39.0 27.5 41.0 68-69 34.32333282643242 35.0 33.0 38.5 27.0 41.0 70-71 34.24472927363408 35.0 33.5 37.5 28.0 40.5 72-73 34.02139436988444 35.0 33.0 37.0 27.5 40.0 74-75 33.77328266454816 35.0 33.0 37.0 27.0 39.5 76-77 33.52056397564425 35.0 33.0 36.0 27.0 39.0 78-79 33.27346602774071 35.0 33.0 36.0 26.0 39.0 80-81 33.11005253748293 35.0 33.0 36.0 26.0 38.0 82-83 32.86651838705373 35.0 33.0 35.0 26.0 37.0 84-85 32.709424601774295 35.0 33.0 35.0 26.0 37.0 86-87 32.55789017010831 35.0 33.0 35.0 26.0 36.0 88-89 32.44874512729294 35.0 33.0 35.0 26.0 36.0 90-91 32.314393970666686 35.0 33.0 35.0 25.0 36.0 92-93 32.16637033825383 35.0 32.5 35.0 25.0 36.0 94-95 32.11140598954471 35.0 32.0 35.0 25.0 35.0 96-97 32.01299934511243 35.0 32.0 35.0 25.0 35.0 98-99 31.932841399760257 35.0 32.0 35.0 25.0 35.0 100 30.982847218911683 34.0 30.0 35.0 22.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.26439069629249445 1101 2 0.34910912042204956 1101 3 0.43647194748825413 1101 4 0.45829703460101 1101 5 0.4253285521281782 1101 6 0.4387666809560713 1101 7 0.42508205909061303 1101 8 0.46725754218253 1101 9 0.600235824688987 1101 10-11 0.6517225095824983 1101 12-13 0.5218232853250626 1101 14-15 0.7415144495674966 1101 16-17 0.7164706211843779 1101 18-19 0.7665503894805923 1101 20-21 0.7382672813832087 1101 22-23 0.7383600422454109 1101 24-25 0.7539137015341879 1101 26-27 0.6857243296028628 1101 28-29 0.6887902170265079 1101 30-31 0.7527399270823523 1101 32-33 0.6815852582764421 1101 34-35 0.7289853013357188 1101 36-37 0.743018920338848 1101 38-39 0.7098718923629406 1101 40-41 0.7516802378892748 1101 42-43 0.7343230561228111 1101 44-45 0.7268000058429962 1101 46-47 0.6670821447727846 1101 48-49 0.6569557781804036 1101 50-51 0.5862305993545718 1101 52-53 0.6053197061589799 1101 54-55 0.6665399160056396 1101 56-57 0.6835510483400498 1101 58-59 0.6612874522501926 1101 60-61 0.6722548181486445 1101 62-63 0.6122118583618175 1101 64-65 0.6394846847198465 1101 66-67 0.6396305053585536 1101 68-69 0.6038588954709709 1101 70-71 0.5918875669953536 1101 72-73 0.624541744954648 1101 74-75 0.5954073508179789 1101 76-77 0.5792277292033816 1101 78-79 0.634324320748803 1101 80-81 0.5800298276655909 1101 82-83 0.5608334895358098 1101 84-85 0.5152983582975779 1101 86-87 0.5249849829395004 1101 88-89 0.5300128561387183 1101 90-91 0.5494715008430759 1101 92-93 0.524253699321406 1101 94-95 0.5478359773338539 1101 96-97 0.49922557947238744 1101 98-99 0.5067777108906562 1101 100 0.6573575743884774 1102 1 0.29372078526843737 1102 2 0.37024881956734035 1102 3 0.4148881882530482 1102 4 0.48881513757950756 1102 5 0.3849023583935036 1102 6 0.3728759999675333 1102 7 0.42048872924099356 1102 8 0.4334534678379143 1102 9 0.5500627651962589 1102 10-11 0.6603490964942722 1102 12-13 0.554733458845682 1102 14-15 0.7342639058537728 1102 16-17 0.6993619468063628 1102 18-19 0.7749345058563151 1102 20-21 0.6910760180922253 1102 22-23 0.719307921958432 1102 24-25 0.7345442621973604 1102 26-27 0.6841235409564064 1102 28-29 0.6920317257433197 1102 30-31 0.7240519727187191 1102 32-33 0.6371136131524224 1102 34-35 0.7075113243631463 1102 36-37 0.7073011529109223 1102 38-39 0.6809614748225172 1102 40-41 0.561838568900356 1102 42-43 0.6603701590105473 1102 44-45 0.6981675756495207 1102 46-47 0.7055804187090828 1102 48-49 0.6452765113753145 1102 50-51 0.6782144084865607 1102 52-53 0.680401911250506 1102 54-55 0.635456575975418 1102 56-57 0.6813685802908509 1102 58-59 0.6095735428941609 1102 60-61 0.5669019449719102 1102 62-63 0.6309557220497624 1102 64-65 0.6554462760542279 1102 66-67 0.6505952212393993 1102 68-69 0.5979578239827603 1102 70-71 0.5817041376600187 1102 72-73 0.5704450940499797 1102 74-75 0.5404183317096667 1102 76-77 0.5699173437280365 1102 78-79 0.5502459340143062 1102 80-81 0.5687958870281093 1102 82-83 0.559590767650036 1102 84-85 0.5684707058548284 1102 86-87 0.6043327870077135 1102 88-89 0.6119068757074828 1102 90-91 0.588078024421641 1102 92-93 0.6339212486441284 1102 94-95 0.5983074313467824 1102 96-97 0.5766824298789075 1102 98-99 0.5593308631642167 1102 100 0.6451659126161999 1103 1 0.2034196762589211 1103 2 0.29156244708088863 1103 3 0.3128729728669235 1103 4 0.40414559871330624 1103 5 0.24532206099710407 1103 6 0.3005534931414289 1103 7 0.24830279697085444 1103 8 0.31923568687424364 1103 9 0.37087610875848753 1103 10-11 0.4870324060819655 1103 12-13 0.42409657936321565 1103 14-15 0.5976006642570724 1103 16-17 0.549619443199937 1103 18-19 0.5822381376540378 1103 20-21 0.521187653174664 1103 22-23 0.6243785768083967 1103 24-25 0.6050838929400442 1103 26-27 0.5213919030108656 1103 28-29 0.5682842709487375 1103 30-31 0.5749461965248059 1103 32-33 0.5648742859728486 1103 34-35 0.653584235616691 1103 36-37 0.6731063214018249 1103 38-39 0.6940077009442547 1103 40-41 0.6091512042885086 1103 42-43 0.5590865836674865 1103 44-45 0.46910072674361203 1103 46-47 0.4888227809953136 1103 48-49 0.477808811821518 1103 50-51 0.5110689399354129 1103 52-53 0.544186751658323 1103 54-55 0.5238739314578353 1103 56-57 0.48489917283407635 1103 58-59 0.5258197011612395 1103 60-61 0.5240144143416146 1103 62-63 0.41262940403581183 1103 64-65 0.3407260218561774 1103 66-67 0.4058942038913642 1103 68-69 0.4029830282677693 1103 70-71 0.39580234706245676 1103 72-73 0.3996755099298568 1103 74-75 0.37821094184371873 1103 76-77 0.34288580704010485 1103 78-79 0.36691963173174 1103 80-81 0.3560726260398752 1103 82-83 0.3405451980510179 1103 84-85 0.37435680964939877 1103 86-87 0.38522258767545736 1103 88-89 0.3961973408685111 1103 90-91 0.3532673226817096 1103 92-93 0.3082775863170468 1103 94-95 0.28705948494672384 1103 96-97 0.3247285275277463 1103 98-99 0.3824031011148996 1103 100 0.47718169957562395 1104 1 0.2835080812701918 1104 2 0.34224371546232035 1104 3 0.32404707278166356 1104 4 0.4370854530461159 1104 5 0.3183065121684052 1104 6 0.3600186679974513 1104 7 0.30203194291402014 1104 8 0.35602924586019213 1104 9 0.48638510036796134 1104 10-11 0.5404814652794698 1104 12-13 0.46736904305525684 1104 14-15 0.5738550118557484 1104 16-17 0.5894682301121819 1104 18-19 0.5549815504970823 1104 20-21 0.4940846981009699 1104 22-23 0.5198512506766946 1104 24-25 0.5738801757881262 1104 26-27 0.5219103403120045 1104 28-29 0.5022687067252889 1104 30-31 0.561188143774551 1104 32-33 0.590305932188258 1104 34-35 0.4857802235921227 1104 36-37 0.4509051162448756 1104 38-39 0.5177888149112491 1104 40-41 0.5969141880292455 1104 42-43 0.5814294521166588 1104 44-45 0.5587587701182102 1104 46-47 0.557806630635497 1104 48-49 0.5127222863583967 1104 50-51 0.45276972848757424 1104 52-53 0.49476487202688446 1104 54-55 0.5324099445216675 1104 56-57 0.4364900030870871 1104 58-59 0.4844745314485692 1104 60-61 0.4499227869052831 1104 62-63 0.4363668700869141 1104 64-65 0.4242841144056584 1104 66-67 0.42938620562213714 1104 68-69 0.46606201143663384 1104 70-71 0.44534186407346255 1104 72-73 0.3940273541521009 1104 74-75 0.3694953338209288 1104 76-77 0.3653097660991449 1104 78-79 0.34634594555735276 1104 80-81 0.31365782055071634 1104 82-83 0.4140931193363784 1104 84-85 0.4618186871255645 1104 86-87 0.41061396461893196 1104 88-89 0.3255852435186455 1104 90-91 0.41448682135024484 1104 92-93 0.4030121447784438 1104 94-95 0.5113307956725706 1104 96-97 0.4825452395942236 1104 98-99 0.5218256678319229 1104 100 0.5536978905044059 1105 1 0.1486828178853834 1105 2 0.24128205001142433 1105 3 0.24249598355039126 1105 4 0.2659617817256503 1105 5 0.1922280417047233 1105 6 0.1294754987376976 1105 7 0.23307889206553511 1105 8 0.23832984221903786 1105 9 0.3776799707104743 1105 10-11 0.4124786790962034 1105 12-13 0.2796041852019968 1105 14-15 0.4332177581502421 1105 16-17 0.3445357939316409 1105 18-19 0.34447709795044545 1105 20-21 0.3373385609955477 1105 22-23 0.35416436625810377 1105 24-25 0.32741358194731873 1105 26-27 0.3400666234175702 1105 28-29 0.37322212007182287 1105 30-31 0.4426850041967185 1105 32-33 0.3484561580565213 1105 34-35 0.3880595661241202 1105 36-37 0.36234554387221607 1105 38-39 0.4590042052910448 1105 40-41 0.39890623732776476 1105 42-43 0.3645928643607377 1105 44-45 0.38687051240768255 1105 46-47 0.3768142036107278 1105 48-49 0.2639974810141723 1105 50-51 0.28310390664664453 1105 52-53 0.3720723662136507 1105 54-55 0.3107085392687523 1105 56-57 0.35475149452007315 1105 58-59 0.3977211726499945 1105 60-61 0.3967758183826433 1105 62-63 0.3700295287174242 1105 64-65 0.40273858088411885 1105 66-67 0.41262304150349394 1105 68-69 0.3328891142422208 1105 70-71 0.38717951339607737 1105 72-73 0.33951475643286955 1105 74-75 0.2846586158502262 1105 76-77 0.31601031957586656 1105 78-79 0.42646681491923744 1105 80-81 0.29992094982078044 1105 82-83 0.34337382514191006 1105 84-85 0.2780036628973832 1105 86-87 0.2221632754030196 1105 88-89 0.2859216007390515 1105 90-91 0.3418244117509701 1105 92-93 0.3496409022420437 1105 94-95 0.29411756070631867 1105 96-97 0.27783802029928495 1105 98-99 0.29879290443350826 1105 100 0.423291692985277 1106 1 0.08946735973122699 1106 2 0.15326363847742996 1106 3 0.17524544649882046 1106 4 0.21570856464185084 1106 5 0.0910643369372437 1106 6 0.18036549960230275 1106 7 0.1275375697357788 1106 8 0.11083965451464195 1106 9 0.2107252514499649 1106 10-11 0.26465032051677895 1106 12-13 0.1748752840128347 1106 14-15 0.3090269578127689 1106 16-17 0.177585854928914 1106 18-19 0.22470226115616754 1106 20-21 0.21734831569053625 1106 22-23 0.300138471750131 1106 24-25 0.28887242695627435 1106 26-27 0.2584822310245585 1106 28-29 0.20683890883597655 1106 30-31 0.33435622744865867 1106 32-33 0.23617550359944772 1106 34-35 0.24278625533064968 1106 36-37 0.19178401619446817 1106 38-39 0.25257491890217665 1106 40-41 0.2946071585101606 1106 42-43 0.2806438031439882 1106 44-45 0.17715637241703064 1106 46-47 0.2037038484203464 1106 48-49 0.15425293145816 1106 50-51 0.14825857975539947 1106 52-53 0.10360256354744735 1106 54-55 0.15309015336406873 1106 56-57 0.11043153933691485 1106 58-59 0.1390718306272447 1106 60-61 0.07898319712917612 1106 62-63 0.06435817149950651 1106 64-65 0.12038625640806089 1106 66-67 0.16259839793852393 1106 68-69 0.14018423001828495 1106 70-71 0.13943940253210485 1106 72-73 0.06580233222816645 1106 74-75 0.06914069362459685 1106 76-77 0.0499660226787455 1106 78-79 0.11747141192390842 1106 80-81 0.0736030149349034 1106 82-83 0.04322238895057495 1106 84-85 0.032231088322980384 1106 86-87 0.04148768721159968 1106 88-89 0.11548962992368672 1106 90-91 0.006119927049702767 1106 92-93 0.10206796679084817 1106 94-95 0.06320627314595839 1106 96-97 0.05296103622508497 1106 98-99 0.07374528594013086 1106 100 0.054136701232259554 1107 1 0.10452356490725734 1107 2 0.15124835915531065 1107 3 0.20697551369584133 1107 4 0.24304794167952082 1107 5 0.10724246873930099 1107 6 0.22876515172393397 1107 7 0.17330556870125235 1107 8 0.1914553699708037 1107 9 0.18945448422972788 1107 10-11 0.33996079936751045 1107 12-13 0.23506920638277506 1107 14-15 0.3498069184894419 1107 16-17 0.32499542118296887 1107 18-19 0.28373358023864625 1107 20-21 0.21327438138956722 1107 22-23 0.41245382802459574 1107 24-25 0.44658839436586106 1107 26-27 0.31054523094338293 1107 28-29 0.3413354639150654 1107 30-31 0.4445413684972621 1107 32-33 0.32534364198056664 1107 34-35 0.3317549040372043 1107 36-37 0.34835367752866375 1107 38-39 0.3639372348631369 1107 40-41 0.3470182550798029 1107 42-43 0.3802073357825364 1107 44-45 0.4248417110969882 1107 46-47 0.3769214732195323 1107 48-49 0.3858657973652271 1107 50-51 0.36430532210641076 1107 52-53 0.3553264841145065 1107 54-55 0.33333963433104685 1107 56-57 0.4021872384351042 1107 58-59 0.3745849954709044 1107 60-61 0.3292791574499887 1107 62-63 0.3478045342331484 1107 64-65 0.38427323617154485 1107 66-67 0.4279127037581105 1107 68-69 0.4031837860261831 1107 70-71 0.39148956231262844 1107 72-73 0.3674315755797224 1107 74-75 0.3860191129419235 1107 76-77 0.32950940222910674 1107 78-79 0.3453019382628 1107 80-81 0.2768448091632649 1107 82-83 0.27527256583947946 1107 84-85 0.2091343460663566 1107 86-87 0.2584357465071392 1107 88-89 0.2856884653499776 1107 90-91 0.3048685164928102 1107 92-93 0.30046294065395784 1107 94-95 0.1771171338558588 1107 96-97 0.2356192451352257 1107 98-99 0.2365299517767845 1107 100 0.3192343499973411 1108 1 -0.02270907494520813 1108 2 -0.07283297324418214 1108 3 -0.07029408341570687 1108 4 0.11999885768214114 1108 5 0.07291644316434542 1108 6 0.10172767908948543 1108 7 0.07347468817290093 1108 8 0.07529173021671909 1108 9 0.05780476915447963 1108 10-11 0.17328623321519387 1108 12-13 0.06962688657694827 1108 14-15 0.224275736811542 1108 16-17 0.17431815041121723 1108 18-19 0.13947980999621734 1108 20-21 0.12213883766855815 1108 22-23 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-0.49336190232157406 2214 76-77 -0.436066674375823 2214 78-79 -0.5063194334348324 2214 80-81 -0.5053272846521253 2214 82-83 -0.48654421323773533 2214 84-85 -0.4744147192981174 2214 86-87 -0.41565676458390044 2214 88-89 -0.45242569921262543 2214 90-91 -0.428422363138516 2214 92-93 -0.4773828200033492 2214 94-95 -0.5012367925626293 2214 96-97 -0.503356161695784 2214 98-99 -0.39327915155542215 2214 100 -0.217614731730027 2215 1 -0.7953788758483356 2215 2 -1.0732548523289402 2215 3 -0.8541943364117337 2215 4 -2.18986610158278 2215 5 -1.0578850156698678 2215 6 -0.726357193862512 2215 7 -0.7840490364542845 2215 8 -0.5565506551172277 2215 9 -1.0113526012296958 2215 10-11 -0.7424968901928679 2215 12-13 -0.9279339374672517 2215 14-15 -0.9721276482781178 2215 16-17 -0.929679315342895 2215 18-19 -0.866628065451458 2215 20-21 -1.0073112709725933 2215 22-23 -0.8514155162662789 2215 24-25 -0.97578180226553 2215 26-27 -0.9392634306762631 2215 28-29 -0.8150457137806697 2215 30-31 -3.5901880513963533 2215 32-33 -1.4595507000397987 2215 34-35 -1.3111974850013937 2215 36-37 -1.0292214872649694 2215 38-39 -0.8806729808247056 2215 40-41 -0.9057111827883517 2215 42-43 -0.828606430002857 2215 44-45 -0.8780244552898537 2215 46-47 -1.107074871230381 2215 48-49 -1.1516270785013774 2215 50-51 -1.0243519204852163 2215 52-53 -0.9721289935153337 2215 54-55 -1.003070321575919 2215 56-57 -0.9675270091971129 2215 58-59 -1.0444269200684033 2215 60-61 -1.031004702997187 2215 62-63 -0.9927014484309566 2215 64-65 -0.9776521503736149 2215 66-67 -0.9183664075635107 2215 68-69 -0.893207383330342 2215 70-71 -1.0213914422561459 2215 72-73 -0.897433962961685 2215 74-75 -1.0955111163112434 2215 76-77 -0.9769881232714113 2215 78-79 -0.8273186887597106 2215 80-81 -0.8827292034797267 2215 82-83 -0.8528157562153069 2215 84-85 -0.8522674502331427 2215 86-87 -0.7567400609824588 2215 88-89 -0.7805580575029651 2215 90-91 -0.873175338680312 2215 92-93 -0.9209854464514322 2215 94-95 -0.9113852980043475 2215 96-97 -1.0795698020658229 2215 98-99 -0.9545324361497514 2215 100 -0.4365988684587876 2216 1 -0.3677562405767283 2216 2 -0.6126715387000878 2216 3 -0.6131507659519393 2216 4 -0.4651607643483544 2216 5 -0.4657853137654939 2216 6 -0.363672161409319 2216 7 -0.5612367999410424 2216 8 -0.5258831008167348 2216 9 -0.6248131449243175 2216 10-11 -0.9838943011943755 2216 12-13 -0.685175066104371 2216 14-15 -0.7759841373597354 2216 16-17 -0.8099012201229598 2216 18-19 -1.0463339280512614 2216 20-21 -1.1248765566135646 2216 22-23 -0.924331492094538 2216 24-25 -0.9830690480586526 2216 26-27 -1.3434827710641457 2216 28-29 -1.282045285139226 2216 30-31 -1.4348414202539388 2216 32-33 -1.5801597967426844 2216 34-35 -1.2345404403189804 2216 36-37 -1.2900446179816 2216 38-39 -1.1284912835883887 2216 40-41 -1.0538621198159746 2216 42-43 -1.1350622043460064 2216 44-45 -1.049583872591647 2216 46-47 -1.1379805334089355 2216 48-49 -1.1428945659352792 2216 50-51 -1.165181742587201 2216 52-53 -1.251902639013899 2216 54-55 -1.2474422563209657 2216 56-57 -1.1703821088041337 2216 58-59 -1.0923553263933954 2216 60-61 -1.1357905742023817 2216 62-63 -1.2681725851299746 2216 64-65 -1.1719046215994169 2216 66-67 -1.1668528117848354 2216 68-69 -1.134575703635086 2216 70-71 -1.0674260914814226 2216 72-73 -1.040273353592461 2216 74-75 -1.087640599422599 2216 76-77 -1.0548266624479439 2216 78-79 -0.9918703856321329 2216 80-81 -1.049718926534723 2216 82-83 -1.0309807308168715 2216 84-85 -1.0737535132951592 2216 86-87 -1.035730975075687 2216 88-89 -1.11991523922147 2216 90-91 -1.1430277438271794 2216 92-93 -1.052323667336882 2216 94-95 -1.1485287328353309 2216 96-97 -1.1346429675311356 2216 98-99 -1.1777569965323487 2216 100 -0.6772650212598244 >>END_MODULE >>Per sequence quality scores pass #Quality Count 1 1.0 2 10.0 3 15.0 4 31.0 5 44.0 6 32.0 7 135.0 8 347.0 9 617.0 10 897.0 11 1435.0 12 2542.0 13 3863.0 14 5533.0 15 7544.0 16 9624.0 17 11544.0 18 12878.0 19 14746.0 20 17179.0 21 19601.0 22 23054.0 23 27011.0 24 31761.0 25 37007.0 26 41331.0 27 45870.0 28 53342.0 29 64608.0 30 75279.0 31 89395.0 32 111420.0 33 144609.0 34 209675.0 35 308164.0 36 498944.0 37 897797.0 38 734016.0 39 252714.0 40 228.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.582025834984194 20.51169513503488 11.812680642095877 24.09359838788505 2 16.013279922440564 19.81728787015187 39.916554358190346 24.252877849217214 3 18.815262707492295 26.65772104523141 28.654771547064257 25.87224470021204 4 12.15290910195041 16.351319965523963 40.43292674481661 31.062844187709015 5 13.10414441671616 38.899349879750396 34.91856851031643 13.077937193217014 6 29.747663760482716 38.5638252539715 18.077412303129474 13.611098682416308 7 26.972606121683164 31.79531785437213 23.618115077246106 17.6139609466986 8 26.595081698267176 34.459059250152876 19.69094426535478 19.254914786225175 9 26.385637884928265 14.758671713686637 21.33906178799906 37.51662861338604 10-11 26.128248320494297 25.442996677608143 27.262562170007925 21.166192831889635 12-13 26.17656333142823 23.430849406391868 27.231030719334065 23.161556542845833 14-15 23.566332000429846 24.56267391740597 24.75361437272019 27.117379709443995 16-17 22.45464468445862 27.159098913943915 26.040151810024675 24.34610459157279 18-19 21.704194750808835 28.23108788032773 27.53038254090511 22.53433482795833 20-21 23.75198295837615 27.274761029629623 26.495146953644618 22.47810905834961 22-23 24.34968076115032 26.75257114877648 26.154268793425363 22.74347929664784 24-25 23.750702436088304 26.739205121990846 27.045200930012065 22.46489151190879 26-27 23.53387754014968 27.113124883479372 25.83515327456255 23.5178443018084 28-29 22.403003821360198 27.70671246901204 26.63427867698054 23.25600503264722 30-31 23.31824710711203 27.304036420012682 26.742131533817233 22.635584939058056 32-33 23.756546297970786 27.380604553930205 25.592892244532912 23.269956903566104 34-35 23.67000239166529 27.235341462154427 26.706551724688964 22.388104421491324 36-37 23.27734281882698 26.650477226691667 26.25220901290583 23.819970941575523 38-39 23.47884531487127 26.934423059610896 26.865613802015776 22.721117823502055 40-41 23.092774444080447 25.867551450358096 27.639848825038154 23.399825280523306 42-43 23.027699126594516 26.180562399614303 26.883575235664992 23.908163238126185 44-45 23.348294852926486 27.120236010202248 26.738730579437014 22.792738557434255 46-47 23.193079299230167 26.95983901456756 26.150929569938803 23.69615211626347 48-49 23.98239747789529 26.554595322465392 26.47107124852574 22.991935951113575 50-51 23.422769128481804 26.228188337794645 26.697338688861617 23.651703844861935 52-53 22.90541360411765 26.94923699723926 26.34644823343706 23.798901165206026 54-55 22.78210793294104 26.738862408947874 26.397302468940953 24.08172718917013 56-57 24.209846943763065 26.163783838064735 25.91369334041155 23.712675877760645 58-59 22.710332776619065 26.796415719053744 27.101849410122608 23.391402094204576 60-61 22.918933014828177 27.341148749508555 26.277288074915482 23.462630160747793 62-63 23.416770056962772 26.57641124424739 25.808402021957278 24.198416676832565 64-65 22.394946176271212 26.95611931115216 26.41680328469243 24.232131227884192 66-67 23.36681104821859 26.466373055773417 26.250686381720534 23.91612951428746 68-69 22.575606206648395 26.44189730036523 26.755071553198874 24.227424939787497 70-71 22.59284044381498 26.28012973790732 26.630563251890905 24.4964665663868 72-73 23.325979018962784 26.257432609607896 27.16426791367286 23.25232045775646 74-75 23.512410479738705 26.627539112571625 26.55567652513226 23.304373882557407 76-77 23.175350653363235 26.613018788521263 26.169621406235848 24.04200915187965 78-79 23.127353680434705 26.64569016366174 26.116265637321096 24.110690518582466 80-81 22.690691089018983 27.04390906794228 26.848020451575934 23.417379391462802 82-83 23.593621178184886 25.944456378016135 28.009347515654543 22.452574928144436 84-85 23.699100031815913 26.494825219565094 26.647725388579644 23.158349360039345 86-87 22.249533356487945 27.282011958006347 27.085992384548348 23.382462300957364 88-89 23.633603319740793 25.76835592394214 26.7924576043212 23.805583151995865 90-91 23.171672412493454 25.98459267845913 26.792115960311314 24.05161894873611 92-93 23.57340924593547 26.23059891175719 26.86338741342706 23.332604428880277 94-95 22.347030704724464 26.30750269173224 27.62678108775946 23.71868551578384 96-97 22.727344762647498 26.259762200694986 27.05418889517917 23.95870414147835 98-99 22.090678457041264 26.943247724382235 26.85933452746389 24.106739291112607 100 23.01935148286959 26.086825333588248 25.93574534396999 24.95807783957217 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 182.0 1 122.5 2 88.5 3 111.0 4 100.5 5 94.5 6 104.0 7 97.5 8 96.0 9 111.0 10 103.5 11 99.5 12 109.0 13 110.5 14 119.5 15 164.5 16 200.0 17 223.0 18 278.0 19 339.0 20 439.0 21 589.0 22 815.5 23 1257.0 24 1868.5 25 2639.5 26 3896.5 27 5420.5 28 7690.5 29 12970.5 30 21446.0 31 30438.0 32 43149.0 33 55993.0 34 65485.0 35 76124.0 36 86080.5 37 102688.0 38 111613.5 39 108193.0 40 102916.0 41 95729.0 42 95318.5 43 103499.0 44 116479.0 45 142236.5 46 167972.5 47 185953.5 48 197724.5 49 193974.0 50 182528.0 51 185663.0 52 189263.5 53 180429.5 54 170194.0 55 145283.5 56 125138.5 57 100915.0 58 76078.0 59 73469.0 60 60402.0 61 39716.0 62 28477.0 63 23521.0 64 17007.5 65 6895.0 66 2740.5 67 1490.0 68 857.5 69 475.0 70 261.5 71 148.5 72 92.5 73 73.5 74 55.0 75 44.0 76 37.0 77 30.0 78 24.5 79 20.5 80 17.0 81 10.5 82 7.5 83 11.5 84 12.5 85 11.0 86 8.0 87 4.5 88 4.5 89 4.5 90 5.0 91 4.5 92 4.0 93 2.5 94 4.5 95 5.5 96 2.0 97 1.5 98 3.0 99 4.5 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.060109037847920675 2 0.008069578408471407 3 0.008149475224396865 4 0.009347927463278756 5 0.004074737612198433 6 0.002423536749738937 7 6.924390713539821E-4 8 0.001358245870732811 9 5.326454395030631E-4 10-11 4.793808955527568E-4 12-13 2.396904477763784E-4 14-15 1.9974203981364865E-4 16-17 4.926970315403333E-4 18-19 0.0014647749586334236 20-21 9.720779270930902E-4 22-23 0.0013981942786955406 24-25 0.0014115104146831172 26-27 0.003808414892446901 28-29 0.004234531244049352 30-31 0.005432983482931244 32-33 0.006391745274036757 34-35 0.0036486212605959826 36-37 0.009001707927601766 38-39 0.006951022985514975 40-41 0.006737964809713748 42-43 0.015260291841762759 44-45 0.020453584876917626 46-47 0.019028758326246932 48-49 0.02225126323524046 50-51 0.01906870673420966 52-53 0.02550040041620915 54-55 0.019801094213526372 56-57 0.0160326277290422 58-59 0.017923519039278073 60-61 0.01701802179212287 62-63 0.012277477380545604 64-65 0.012197580564620145 66-67 0.014767594810222426 68-69 0.005965628922434307 70-71 0.009334611327291182 72-73 0.009521037231117252 74-75 0.012130999884682261 76-77 0.011265451045489785 78-79 0.01287670349998655 80-81 0.01101244446172583 82-83 0.006990971393477703 84-85 0.01151845762925374 86-87 0.00988057290278182 88-89 0.008868546567726001 90-91 0.013955310514980255 92-93 0.009321295191303603 94-95 0.007736675008781992 96-97 0.009973785854694857 98-99 0.009800676086856362 100 0.261182691260327 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3754843.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.18544166057632 #Duplication Level Percentage of deduplicated Percentage of total 1 76.221336724152 52.7340684521996 2 14.698883198858132 20.33897452060449 3 4.8427000820584825 10.051330320207759 4 1.9798416349145924 5.479048717182542 5 0.9241552953546587 3.196904613603621 6 0.49070188110236274 2.0369655820645547 7 0.27961638039524794 1.3541767941223861 8 0.16258097647005904 0.899858933015104 9 0.1122256084138355 0.6987940455364289 >10 0.2845577202279901 2.7933248233189767 >50 0.0022317829681614185 0.10866122759732816 >100 9.046136057997554E-4 0.09739879039160175 >500 1.5846088719404745E-4 0.08427035997208089 >1k 1.0564059146259402E-4 0.12622282018356779 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.6632271975153155E-5 0.0 22-23 0.0 0.0 0.0 1.198452238881892E-4 0.0 24-25 0.0 0.0 0.0 3.3290339968941444E-4 0.0 26-27 0.0 0.0 0.0 6.791229353664055E-4 0.0 28-29 0.0 0.0 0.0 0.002969498325229577 0.0 30-31 0.0 0.0 0.0 0.011145605821601595 0.0 32-33 0.0 0.0 0.0 0.026312684711451317 0.0 34-35 0.0 0.0 0.0 0.05085432333655495 0.0 36-37 0.0 0.0 0.0 0.0918680221782908 0.0 38-39 0.0 0.0 0.0 0.154413912911938 0.0 40-41 0.0 0.0 0.0 0.22680042814040427 0.0 42-43 0.0 0.0 0.0 0.3008115119593549 0.0 44-45 0.0 0.0 0.0 0.38921733878087583 0.0 46-47 0.0 0.0 0.0 0.5024178108112642 0.0 48-49 0.0 0.0 0.0 0.6363381904383218 0.0 50-51 0.0 0.0 0.0 0.7829754799335151 0.0 52-53 0.0 0.0 0.0 0.9299190405564227 0.0 54-55 0.0 0.0 0.0 1.0853582959394041 0.0 56-57 0.0 0.0 0.0 1.2764981119050782 0.0 58-59 0.0 0.0 0.0 1.5095038594156933 0.0 60-61 0.0 0.0 0.0 1.7515246309898975 0.0 62-63 0.0 0.0 0.0 2.009644610973082 0.0 64-65 0.0 0.0 0.0 2.2758608016367132 0.0 66-67 0.0 0.0 0.0 2.5816658645914092 0.0 68-69 0.0 0.0 0.0 2.9283514650279656 0.0 70-71 0.0 0.0 0.0 3.3115499103424564 0.0 72-73 0.0 0.0 0.0 3.70649318759799 0.0 74-75 0.0 0.0 0.0 4.097548153145151 0.0 76-77 0.0 0.0 0.0 4.546235355246544 0.0 78-79 0.0 0.0 0.0 5.031049234282232 2.6632271975153155E-5 80-81 0.0 0.0 0.0 5.551550357764626 2.6632271975153155E-5 82-83 0.0 0.0 0.0 6.1012537674677745 2.6632271975153155E-5 84-85 0.0 0.0 0.0 6.63399241992275 2.6632271975153155E-5 86-87 0.0 0.0 0.0 7.203616769063314 2.6632271975153155E-5 88 0.0 0.0 0.0 7.663542789938221 2.6632271975153155E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCACC 4250 0.0 42.3512 94 GTACTAG 4640 0.0 35.667427 1 TAGTACT 6180 0.0 31.5593 4 CTGTGCG 6145 0.0 31.199957 9 TAGGCAT 5790 0.0 31.085587 5 GCATCGT 4865 0.0 30.71567 8 CGTTAAC 830 0.0 30.588825 1 ACTGTGC 6690 0.0 30.063072 8 GTACTGT 6635 0.0 29.24993 6 CTAACGC 2565 0.0 28.94926 3 CGCCTAC 2650 0.0 28.549314 7 CTAGGCA 6500 0.0 28.487259 4 TCTAACG 2605 0.0 28.324333 2 TACTGTG 7395 0.0 28.150187 7 CAACGCA 8595 0.0 27.775167 5 CTAGTAC 7540 0.0 27.674475 3 TACCCCG 240 0.0 27.412943 5 TAACGCC 2735 0.0 27.32169 4 GTATCAA 9895 0.0 27.036068 1 TATTCTC 1785 0.0 26.853493 5 >>END_MODULE