FastQCFastQC Report
Mon 16 Jan 2017
ERR1146644_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146644_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3754843
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66870.17809000269784914No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59430.1582755923483352No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC41000.040.3736194
GTATCAA140500.034.4219171
GTACTAG48450.034.3738141
GGTATCA90250.032.997021
TAGGCAT60500.030.9957245
CAACGCA153350.029.4832635
CTGTGCG60400.029.104459
CAGTACG5200.028.9248669
TAGTACT61750.028.796414
CTAGGCA67800.028.7246114
ATCAACG158450.028.5605353
ACTGTGC65050.027.8888158
CTAACGC22150.027.3967783
TATCAAC177350.027.2166332
TCTAACG22500.027.1818542
TAACGCC22550.026.9108034
GTACTGT67350.026.5867866
GCATCGT57300.026.5754368
AACGCAG182100.026.4798766
CCGTGTT5550.026.2538769
Produced by FastQC (version 0.11.5)