##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146644_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3754843
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10661750704357	33.0	31.0	34.0	30.0	34.0
2	32.20943938268524	33.0	31.0	34.0	30.0	34.0
3	32.37361002843528	34.0	31.0	34.0	30.0	34.0
4	35.97172504948942	37.0	35.0	37.0	35.0	37.0
5	35.87847507871834	37.0	35.0	37.0	35.0	37.0
6	35.91068121889517	37.0	35.0	37.0	35.0	37.0
7	35.844685117327145	37.0	35.0	37.0	35.0	37.0
8	35.85394862048826	37.0	35.0	37.0	35.0	37.0
9	37.56625403512211	39.0	37.0	39.0	35.0	39.0
10-11	37.50374542956922	39.0	37.0	39.0	34.5	39.0
12-13	37.46291442811324	39.0	37.0	39.0	34.0	39.0
14-15	38.68261828790178	40.0	38.0	41.0	34.0	41.0
16-17	38.68481864621237	40.0	38.0	41.0	34.0	41.0
18-19	38.73968405603111	40.0	38.0	41.0	34.0	41.0
20-21	38.733031847137156	40.0	38.0	41.0	34.0	41.0
22-23	38.69207873671416	40.0	38.0	41.0	34.0	41.0
24-25	38.61118507484866	40.0	38.0	41.0	34.0	41.0
26-27	38.46749398044073	40.0	38.0	41.0	34.0	41.0
28-29	38.41763091026709	40.0	38.0	41.0	34.0	41.0
30-31	38.29346539921909	40.0	38.0	41.0	34.0	41.0
32-33	38.16117398250739	40.0	37.0	41.0	33.5	41.0
34-35	38.06368908100818	40.0	37.0	41.0	33.0	41.0
36-37	38.06169099480324	40.0	37.0	41.0	33.0	41.0
38-39	37.969117483740334	40.0	37.0	41.0	33.0	41.0
40-41	37.85290237168371	40.0	37.0	41.0	33.0	41.0
42-43	37.717485125210295	40.0	37.0	41.0	33.0	41.0
44-45	37.50178209847922	40.0	36.0	41.0	32.0	41.0
46-47	37.26247102741712	40.0	36.0	41.0	31.5	41.0
48-49	36.99733544118889	39.0	35.5	41.0	31.0	41.0
50-51	36.770357375794404	39.0	35.0	41.0	31.0	41.0
52-53	36.57494707501752	39.0	35.0	41.0	30.5	41.0
54-55	36.377655470548305	38.5	35.0	41.0	30.0	41.0
56-57	36.13581446680993	38.0	35.0	40.0	30.0	41.0
58-59	35.838121860221584	37.0	35.0	40.0	30.0	41.0
60-61	35.64740882641431	37.0	35.0	40.0	30.0	41.0
62-63	35.4136331399209	36.5	35.0	40.0	29.5	41.0
64-65	35.15664662943297	36.0	34.0	39.0	29.0	41.0
66-67	34.89782062791973	35.5	34.0	39.0	29.0	41.0
68-69	34.64295591054007	35.0	34.0	39.0	29.0	41.0
70-71	34.43564737593556	35.0	34.0	37.5	29.0	40.5
72-73	34.19873747051474	35.0	34.0	37.0	29.0	40.0
74-75	33.948018732074814	35.0	34.0	36.5	29.0	39.0
76-77	33.71400322197226	35.0	34.0	36.0	29.0	39.0
78-79	33.50222486000081	35.0	33.0	36.0	28.5	39.0
80-81	33.387999578144814	35.0	33.0	36.0	29.0	37.5
82-83	33.23868827538195	35.0	33.0	35.0	29.0	37.0
84-85	33.12227834825583	35.0	33.0	35.0	29.0	37.0
86-87	33.003145670804344	35.0	33.0	35.0	29.0	36.0
88-89	32.91540818084804	35.0	33.0	35.0	29.0	36.0
90-91	32.857525600937244	35.0	33.0	35.0	29.0	36.0
92-93	32.79813323752818	35.0	33.0	35.0	29.0	36.0
94-95	32.763026310287806	35.0	33.0	35.0	29.0	35.0
96-97	32.67740555330809	35.0	33.0	35.0	28.5	35.0
98-99	32.61233612164344	35.0	33.0	35.0	27.0	35.0
100	31.367030525643816	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.38941017582384063
1101	2	0.4461990559829587
1101	3	0.35819536052883905
1101	4	0.23330171708757064
1101	5	0.262444545880129
1101	6	0.29286451619627485
1101	7	0.2673638034027377
1101	8	0.33091376052950494
1101	9	0.34650631193932213
1101	10-11	0.42359051073582776
1101	12-13	0.4065603325383478
1101	14-15	0.5414216755490742
1101	16-17	0.528659807226525
1101	18-19	0.4849831758930847
1101	20-21	0.4990721312253541
1101	22-23	0.4562613604104513
1101	24-25	0.4976242125815915
1101	26-27	0.5305799515580034
1101	28-29	0.5958859787578206
1101	30-31	0.5791959372254496
1101	32-33	0.6335415500755914
1101	34-35	0.592041987238872
1101	36-37	0.5053929378996429
1101	38-39	0.5445821512890205
1101	40-41	0.48269165680093096
1101	42-43	0.4316631293378208
1101	44-45	0.4681628188665172
1101	46-47	0.5245352064306701
1101	48-49	0.5026250769582816
1101	50-51	0.570314901368981
1101	52-53	0.5654287736584251
1101	54-55	0.5726953440740914
1101	56-57	0.6069697860765402
1101	58-59	0.58999724554171
1101	60-61	0.5438529655291902
1101	62-63	0.5678428470851813
1101	64-65	0.5404933542600787
1101	66-67	0.4698329907759913
1101	68-69	0.4153299001561308
1101	70-71	0.4761025458813535
1101	72-73	0.5382502376945411
1101	74-75	0.5219605021263334
1101	76-77	0.46902527689442053
1101	78-79	0.48858404794093957
1101	80-81	0.49266236650700534
1101	82-83	0.5224545120714268
1101	84-85	0.456969661987344
1101	86-87	0.427931878203907
1101	88-89	0.5094419918530662
1101	90-91	0.4953645349988278
1101	92-93	0.47796138074151173
1101	94-95	0.4962479994032378
1101	96-97	0.47247683734767065
1101	98-99	0.5148350590021096
1101	100	0.7402157313171891
1102	1	0.4081289678417974
1102	2	0.4526461234738264
1102	3	0.37256600863667444
1102	4	0.2767673968533444
1102	5	0.31480405490319896
1102	6	0.24027767959266555
1102	7	0.25943152683112913
1102	8	0.2812322723577978
1102	9	0.33948647756627537
1102	10-11	0.3395233687702657
1102	12-13	0.40790389084835965
1102	14-15	0.5327789128289524
1102	16-17	0.4883218391159545
1102	18-19	0.5036392804065954
1102	20-21	0.5296050439810003
1102	22-23	0.4932887852524104
1102	24-25	0.5057714724762832
1102	26-27	0.5574325264068491
1102	28-29	0.5045969464156741
1102	30-31	0.584569354178555
1102	32-33	0.5613219133172791
1102	34-35	0.5824248444670204
1102	36-37	0.5514878945258204
1102	38-39	0.6155564173937549
1102	40-41	0.5660962736398503
1102	42-43	0.5747968102161423
1102	44-45	0.6422712736133747
1102	46-47	0.6082667378555868
1102	48-49	0.6060035186405415
1102	50-51	0.6353782005888533
1102	52-53	0.677972351719248
1102	54-55	0.5582371033809963
1102	56-57	0.6124985141122536
1102	58-59	0.5515032196292395
1102	60-61	0.5700685200532192
1102	62-63	0.6050027602935515
1102	64-65	0.521151452112953
1102	66-67	0.577019544257972
1102	68-69	0.5304441823253967
1102	70-71	0.5027016219911431
1102	72-73	0.5329999301376063
1102	74-75	0.5701513004336292
1102	76-77	0.4939531970926083
1102	78-79	0.5424653157085118
1102	80-81	0.47376439117846303
1102	82-83	0.42647401219996084
1102	84-85	0.4686516775137193
1102	86-87	0.4935345098596571
1102	88-89	0.4961518410978556
1102	90-91	0.519534391181736
1102	92-93	0.4959353359242229
1102	94-95	0.4751250091516326
1102	96-97	0.4477864566461065
1102	98-99	0.473417326531262
1102	100	0.7563936386880137
1103	1	0.26421729846496333
1103	2	0.3334683709944315
1103	3	0.26411338341099366
1103	4	0.201440852831972
1103	5	0.20571155598997137
1103	6	0.21662123080379558
1103	7	0.26696108739614033
1103	8	0.2118929586547793
1103	9	0.28351621091422174
1103	10-11	0.3274671049823681
1103	12-13	0.2716351134856012
1103	14-15	0.40173491846498166
1103	16-17	0.37284699017648393
1103	18-19	0.4067119850547485
1103	20-21	0.434677337515879
1103	22-23	0.40513579553077506
1103	24-25	0.37788587942703344
1103	26-27	0.4730936741574965
1103	28-29	0.502500675118668
1103	30-31	0.37082696226489986
1103	32-33	0.4577949783187947
1103	34-35	0.5111509648948882
1103	36-37	0.45988488312109155
1103	38-39	0.41781932328289884
1103	40-41	0.4252847994893827
1103	42-43	0.47427607547702166
1103	44-45	0.46870019375410266
1103	46-47	0.45875050386291605
1103	48-49	0.3877962346991808
1103	50-51	0.4238654033772917
1103	52-53	0.3221852113843653
1103	54-55	0.32074892157663726
1103	56-57	0.3548680761035783
1103	58-59	0.3960521276882645
1103	60-61	0.3966480025643975
1103	62-63	0.4289742429809138
1103	64-65	0.4078257056723089
1103	66-67	0.3310040394145446
1103	68-69	0.29371149981653843
1103	70-71	0.335533801777693
1103	72-73	0.3598156454668384
1103	74-75	0.3390729985621732
1103	76-77	0.2641084972288965
1103	78-79	0.2622775932078767
1103	80-81	0.3044593325450009
1103	82-83	0.2743970380565699
1103	84-85	0.32711197178482365
1103	86-87	0.3492764224907532
1103	88-89	0.3181352712403225
1103	90-91	0.2805861411137016
1103	92-93	0.31537310181723655
1103	94-95	0.3057032761518741
1103	96-97	0.31427912556306126
1103	98-99	0.29378763523852314
1103	100	0.5315398487113114
1104	1	0.24559390376249723
1104	2	0.28844685177691787
1104	3	0.23046524416366765
1104	4	0.2219901692354469
1104	5	0.1190133750821687
1104	6	0.18299731936755137
1104	7	0.24988890353532156
1104	8	0.19832850756674958
1104	9	0.2347695395567797
1104	10-11	0.2724443421967422
1104	12-13	0.29252188417958536
1104	14-15	0.295901404159153
1104	16-17	0.36138149404959563
1104	18-19	0.3824536526120781
1104	20-21	0.3866013317178627
1104	22-23	0.35664690745228
1104	24-25	0.34925739246771315
1104	26-27	0.4544049621596713
1104	28-29	0.42692616683912377
1104	30-31	0.4440099679191931
1104	32-33	0.4909943194485251
1104	34-35	0.5419805548000696
1104	36-37	0.46939278473145407
1104	38-39	0.4196126702755052
1104	40-41	0.49865137497787515
1104	42-43	0.4603533177767076
1104	44-45	0.432992390872073
1104	46-47	0.44491192834210835
1104	48-49	0.44063273722790086
1104	50-51	0.4643518738483223
1104	52-53	0.49629410113608685
1104	54-55	0.42824295579362115
1104	56-57	0.4200207453837308
1104	58-59	0.40748726018473747
1104	60-61	0.4257398937305439
1104	62-63	0.46896998986438376
1104	64-65	0.4094362714964319
1104	66-67	0.37099868216601095
1104	68-69	0.40650274933687314
1104	70-71	0.37824490897982344
1104	72-73	0.37873687189377847
1104	74-75	0.41815650147966466
1104	76-77	0.3032105281125439
1104	78-79	0.31522510832407136
1104	80-81	0.24695820349114683
1104	82-83	0.23563985914794472
1104	84-85	0.2799470168592748
1104	86-87	0.32137809803316486
1104	88-89	0.2932383719332563
1104	90-91	0.34065686928687455
1104	92-93	0.3015163554381317
1104	94-95	0.3192662760949645
1104	96-97	0.37657390685469494
1104	98-99	0.3007422000168134
1104	100	0.45553420842641756
1105	1	0.19206486241301235
1105	2	0.1960572348810672
1105	3	0.15689886910134732
1105	4	0.21488723173371937
1105	5	0.16361546872575872
1105	6	0.1539257718385585
1105	7	0.18626465918160307
1105	8	0.1697469068870845
1105	9	0.2079299721191461
1105	10-11	0.24327593905416478
1105	12-13	0.23896869351737138
1105	14-15	0.2809508518906654
1105	16-17	0.29314175219587213
1105	18-19	0.3027051142838033
1105	20-21	0.29669047687686856
1105	22-23	0.3270834981504862
1105	24-25	0.2798982106664951
1105	26-27	0.29398996845475267
1105	28-29	0.2738877015281034
1105	30-31	0.33913717765349816
1105	32-33	0.3157780615172001
1105	34-35	0.24317009006546897
1105	36-37	0.33504444367677166
1105	38-39	0.31496657047461696
1105	40-41	0.3904194137925856
1105	42-43	0.33390725799468157
1105	44-45	0.2925548211312403
1105	46-47	0.26309002029837814
1105	48-49	0.3218260214879223
1105	50-51	0.33576324266388724
1105	52-53	0.4199794461385835
1105	54-55	0.30008059265404086
1105	56-57	0.23760630530446747
1105	58-59	0.2867315474892749
1105	60-61	0.24931636773452936
1105	62-63	0.3353067658755222
1105	64-65	0.2827623966120001
1105	66-67	0.29907465794034493
1105	68-69	0.2869199272703753
1105	70-71	0.2570544630619267
1105	72-73	0.21195615582972493
1105	74-75	0.23802036440251584
1105	76-77	0.20165201444939385
1105	78-79	0.20278932779047665
1105	80-81	0.16338913446584513
1105	82-83	0.2250809559042537
1105	84-85	0.16837700970384617
1105	86-87	0.19417834868752948
1105	88-89	0.25416318817734407
1105	90-91	0.27610297517904314
1105	92-93	0.2197748225078513
1105	94-95	0.28159081021308197
1105	96-97	0.22329013376728568
1105	98-99	0.2760198388444337
1105	100	0.36901577730315793
1106	1	0.15129520171748823
1106	2	0.24227321250970135
1106	3	0.1654780363459949
1106	4	0.14593236948115162
1106	5	0.1423487253532727
1106	6	0.154305876560052
1106	7	0.19840574198777716
1106	8	0.11374856222206375
1106	9	0.10822095027034351
1106	10-11	0.16081999581013662
1106	12-13	0.13940502416537015
1106	14-15	0.2180330989277337
1106	16-17	0.18266099840444383
1106	18-19	0.1529720456599719
1106	20-21	0.18868432299271376
1106	22-23	0.23145273393156884
1106	24-25	0.2458818517336212
1106	26-27	0.21283159797990692
1106	28-29	0.22201126032491914
1106	30-31	0.20667817920123355
1106	32-33	0.2855619036045667
1106	34-35	0.24753894877031257
1106	36-37	0.26757762222872117
1106	38-39	0.23643679249710203
1106	40-41	0.21556518493308374
1106	42-43	0.25491475997091584
1106	44-45	0.21552411418040407
1106	46-47	0.2328368525017126
1106	48-49	0.28355162566499814
1106	50-51	0.32176363422935594
1106	52-53	0.29188820782260194
1106	54-55	0.20428706214210024
1106	56-57	0.19685876443534056
1106	58-59	0.25880208972371577
1106	60-61	0.22002613981658925
1106	62-63	0.2312777419317129
1106	64-65	0.2742081846882698
1106	66-67	0.24524255686443297
1106	68-69	0.2369139474944859
1106	70-71	0.16481499483086992
1106	72-73	0.16167004107123262
1106	74-75	0.11946275641738424
1106	76-77	0.14897669122083101
1106	78-79	0.2090180779116153
1106	80-81	0.1475889573172111
1106	82-83	0.13315511415132875
1106	84-85	0.11308381244415955
1106	86-87	0.10741938662461337
1106	88-89	0.16405960444480883
1106	90-91	0.15201057095521264
1106	92-93	0.16180736792801298
1106	94-95	0.19185708779249921
1106	96-97	0.11205966631627717
1106	98-99	0.14923071602707694
1106	100	0.2875218535155497
1107	1	0.1201644884606381
1107	2	0.1816943957114816
1107	3	0.17314648668967436
1107	4	0.14169748361886292
1107	5	0.06591376836729523
1107	6	0.08218854512838192
1107	7	0.14362663838223
1107	8	0.14723335982868235
1107	9	0.15340575977023718
1107	10-11	0.16992507858168437
1107	12-13	0.08620332845298861
1107	14-15	0.21309119428538992
1107	16-17	0.21412081210473843
1107	18-19	0.14364383119491464
1107	20-21	0.17722371050779628
1107	22-23	0.158919300016656
1107	24-25	0.13921863407814072
1107	26-27	0.182351195104566
1107	28-29	0.18016950309786495
1107	30-31	0.16837502604318644
1107	32-33	0.3003138744905769
1107	34-35	0.217813541897506
1107	36-37	0.17662007946012181
1107	38-39	0.22925050365280697
1107	40-41	0.2239370848936275
1107	42-43	0.2184003770102052
1107	44-45	0.19288072349189633
1107	46-47	0.24349365109094379
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2216	72-73	-0.809005323071375
2216	74-75	-0.7756404468119982
2216	76-77	-0.8573863643145856
2216	78-79	-0.7285894591672388
2216	80-81	-0.8027507055725209
2216	82-83	-0.7337830843137283
2216	84-85	-0.7439912102985602
2216	86-87	-0.7579748037946175
2216	88-89	-0.6920966195542491
2216	90-91	-0.7446891007208905
2216	92-93	-0.8071928776244199
2216	94-95	-0.7667113868133271
2216	96-97	-0.8863511746296169
2216	98-99	-0.8557712820533823
2216	100	-0.27258179852671915
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	7.0
13	41.0
14	179.0
15	540.0
16	1316.0
17	2760.0
18	4890.0
19	7826.0
20	11201.0
21	16064.0
22	22646.0
23	26451.0
24	26277.0
25	26287.0
26	28740.0
27	35358.0
28	45321.0
29	56810.0
30	71306.0
31	88878.0
32	113199.0
33	145474.0
34	207538.0
35	307267.0
36	500296.0
37	956102.0
38	786498.0
39	265237.0
40	332.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.19968344881531	21.53086027831257	13.074741074393788	25.19471519847834
2	14.595268800708258	20.821794050231105	39.27766884374918	25.30526830531145
3	17.41247237234686	26.95928964273606	29.50211233865171	26.12612564626537
4	11.793436075250243	17.182162186368462	39.15401584844181	31.870385889939477
5	12.573604905883998	39.036427919859904	35.04417825777198	13.345788916484116
6	29.727828760837337	38.93503375108726	17.48923123795888	13.847906250116516
7	26.155701399872854	32.48923190089807	23.67114879165323	17.683917907575847
8	26.775020952939975	34.27440241842336	19.3581728983076	19.59240373032907
9	26.34248622379151	14.180939123153752	21.350559797040784	38.12601485601395
10-11	26.025602135695152	25.613547623695588	27.044872448728213	21.315977791881046
12-13	26.238455364872195	23.516187223997047	27.60062143983407	22.644735971296694
14-15	23.4833898214797	24.38603266768323	25.515165236151528	26.61541227468554
16-17	21.944006713463118	26.871230035450218	26.81094788783446	24.373815363252206
18-19	21.291045174609902	27.180308409822356	29.270383317112053	22.25826309845569
20-21	23.109581412591684	26.418960792768164	28.414197344602695	22.057260450037457
22-23	23.54380729101057	25.900497038091874	28.187583342366114	22.368112328531446
24-25	23.00554244212075	25.934013752372604	29.03218589965013	22.028257905856517
26-27	22.860023708048512	26.261550749259023	27.882071234403146	22.99635430828932
28-29	21.882153261800827	26.822839197271364	28.5869742090415	22.708033331886313
30-31	22.842046924465283	26.377467713030878	28.57526932550842	22.20521603699542
32-33	23.370417884316335	26.4948095033534	27.64471111042459	22.49006150190567
34-35	23.131925888778838	26.43695089248738	28.513522402934022	21.917600815799755
36-37	22.85937121738512	25.791078348681957	28.05364964660307	23.295900787329856
38-39	22.935126715018445	25.980207428113506	28.826904347265653	22.2577615096024
40-41	22.484348879567	25.198297239059	29.341066990017957	22.976286891356043
42-43	22.687899334273098	25.387799170298198	28.45145589309593	23.47284560233277
44-45	23.01740711928568	26.27950090057028	28.12076297198045	22.58232900816359
46-47	22.984462998852415	26.276451505429122	27.228062531509305	23.511022964209154
48-49	23.96735362836742	26.087309109861582	27.280781113884125	22.664556147886874
50-51	23.117211558512565	25.889764232485884	27.3295714361426	23.663452772858946
52-53	22.535163254495593	26.5377939903213	26.93510753978262	23.991935215400485
54-55	22.52782606356644	26.51587563048575	26.820721931649338	24.135576374298473
56-57	23.963851484602685	25.86377113503814	26.401836241888144	23.770541138471035
58-59	22.402777213691387	26.524907502778078	27.568959283911376	23.503355999619156
60-61	22.781977835025327	27.02545752245833	26.627984179365153	23.564580463151188
62-63	23.302092790564082	26.192173680763748	26.170335217744125	24.335398310928046
64-65	22.27500742042172	26.568665167002433	26.7465821783908	24.40974523418505
66-67	23.094577233200063	26.119449691011802	26.654532980934455	24.131440094853676
68-69	22.500141816848267	25.851586870609506	27.249062077961717	24.399209234580514
70-71	22.4387084385814	25.73160973516836	27.088079733260145	24.74160209299009
72-73	23.09805076161329	25.59402560784782	27.62639701898264	23.68152661155625
74-75	23.146103743486552	26.184338379236227	27.03331190758779	23.63624596968943
76-77	22.867722343998366	26.183162773491976	26.43122923596888	24.51788564654078
78-79	22.664852120961132	26.241420245989012	26.594817226022133	24.498910407027726
80-81	22.25204888726373	26.798390771598175	27.17655571750936	23.773004623628736
82-83	23.200091188899243	25.581708742549292	28.310864129339098	22.907335939212373
84-85	23.376010661431117	26.155381197030074	26.784808312890846	23.68379982864796
86-87	21.814134439176286	26.82334521043889	27.293444759208306	24.069075591176514
88-89	22.954954441503947	25.525301590505915	27.03726893507931	24.48247503291083
90-91	22.49930290028105	25.60724909137346	27.12888395067384	24.764564057671652
92-93	22.94624568856807	25.900497038091874	26.964549516451154	24.188707756888903
94-95	21.683369983778285	25.906981996317825	27.74263797447723	24.667010045426665
96-97	22.055036655327534	25.829522033278092	27.207196146416774	24.908245164977604
98-99	21.428778779831806	26.510535859954732	27.076951020322287	24.983734339891175
100	22.335478145698893	25.682931086632387	26.11299505791526	25.868595709753457
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	126.0
1	80.0
2	53.5
3	68.5
4	43.5
5	36.0
6	79.0
7	179.5
8	292.5
9	363.0
10	324.0
11	225.5
12	222.5
13	277.5
14	360.5
15	526.0
16	726.5
17	997.5
18	1436.0
19	1946.0
20	2492.0
21	3168.5
22	3892.5
23	4722.0
24	5669.0
25	6655.5
26	7995.0
27	9394.0
28	11552.0
29	16675.0
30	25000.0
31	33098.5
32	45392.0
33	58559.5
34	66862.5
35	75681.5
36	84522.5
37	100658.5
38	109510.0
39	106064.0
40	100975.5
41	94373.0
42	93960.5
43	100988.0
44	113418.5
45	139884.0
46	165562.5
47	183991.5
48	197787.0
49	192820.5
50	179706.5
51	181949.5
52	184229.5
53	175560.5
54	169496.5
55	145605.0
56	123425.5
57	100152.5
58	75465.5
59	74112.5
60	61174.0
61	39048.0
62	26935.5
63	22047.0
64	15877.0
65	5973.0
66	2091.0
67	1123.0
68	649.5
69	344.0
70	145.0
71	60.0
72	27.0
73	9.0
74	4.0
75	1.5
76	1.0
77	2.5
78	1.5
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.007377139337117424
3	0.0
4	0.08807292342183148
5	0.00447422169182573
6	2.6632271975153155E-5
7	5.326454395030631E-5
8	0.0
9	0.0
10-11	0.0
12-13	3.8616794363972076E-4
14-15	0.009800676086856362
16-17	0.0
18-19	3.994840796272974E-5
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	1.3316135987576578E-5
60-61	0.0
62-63	0.0
64-65	6.658067993788288E-5
66-67	1.8642590382607208E-4
68-69	0.0
70-71	6.258583914160992E-4
72-73	4.660647595651802E-4
74-75	3.8616794363972076E-4
76-77	6.658067993788288E-5
78-79	1.3316135987576578E-5
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0633315427569142
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3754843.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.06170867261282
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.95668959279921	50.267236805357705
2	15.416143828884838	20.67665892615546
3	5.121338324925508	10.30337096280124
4	2.0822583917376973	5.58559222591267
5	0.9834745925565549	3.2976743306472143
6	0.5078051513839388	2.0432568674737057
7	0.30295173277212006	1.4221522591519016
8	0.18518838664186899	0.9935239707622563
9	0.11356131696996619	0.6854054359606303
>10	0.32090920169335235	3.0704282435697743
>50	0.004857433892957873	0.22452969650796312
>100	0.004004116958560743	0.4922427430042575
>500	5.998144411885775E-4	0.28636245113395303
>1k	1.0905717110836281E-4	0.1897137525614845
>5k	1.0905717110836281E-4	0.4618513289999131
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6687	0.17809000269784914	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5943	0.1582755923483352	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	2.6632271975153155E-5	0.0
22-23	0.0	0.0	0.0	9.321295191303604E-5	0.0
24-25	0.0	0.0	0.0	2.796388557391081E-4	0.0
26-27	0.0	0.0	0.0	5.992261194409461E-4	0.0
28-29	0.0	0.0	0.0	0.0029561821892420004	0.0
30-31	0.0	0.0	0.0	0.011158921957589173	0.0
32-33	0.0	0.0	0.0	0.026272736303488586	0.0
34-35	0.0	0.0	0.0	0.05097416856044314	0.0
36-37	0.0	0.0	0.0	0.09228082239390568	0.0
38-39	0.0	0.0	0.0	0.154760132447615	0.0
40-41	0.0	0.0	0.0	0.22735970585188248	0.0
42-43	0.0	0.0	0.0	0.30117104763101943	0.0
44-45	0.0	0.0	0.0	0.3897633003563664	0.0
46-47	0.0	0.0	0.0	0.5030969337466307	0.0
48-49	0.0	0.0	0.0	0.637563274949179	0.0
50-51	0.0	0.0	0.0	0.7840940353564716	0.0
52-53	0.0	1.3316135987576578E-5	0.0	0.9313571832430809	0.0
54-55	0.0	2.6632271975153155E-5	0.0	1.0878883617770438	0.0
56-57	0.0	2.6632271975153155E-5	0.0	1.2797339329500594	0.0
58-59	0.0	2.6632271975153155E-5	0.0	1.5135652808919042	0.0
60-61	0.0	2.6632271975153155E-5	0.0	1.7572905178725182	0.0
62-63	0.0	2.6632271975153155E-5	0.0	2.01663558236656	0.0
64-65	0.0	2.6632271975153155E-5	0.0	2.284276599580861	0.0
66-67	0.0	2.6632271975153155E-5	0.0	2.5914532245422777	0.0
68-69	0.0	2.6632271975153155E-5	0.0	2.9401096131049953	0.0
70-71	0.0	2.6632271975153155E-5	0.0	3.3253187949536107	0.0
72-73	0.0	2.6632271975153155E-5	0.0	3.7220863828394424	0.0
74-75	0.0	2.6632271975153155E-5	0.0	4.115285246280603	0.0
76-77	0.0	2.6632271975153155E-5	0.0	4.5674080114667905	0.0
78-79	0.0	2.6632271975153155E-5	0.0	5.0558172472191245	0.0
80-81	0.0	2.6632271975153155E-5	0.0	5.579820514466251	0.0
82-83	0.0	2.6632271975153155E-5	0.0	6.13430441698894	0.0
84-85	0.0	2.6632271975153155E-5	0.0	6.671530607271729	0.0
86-87	0.0	2.6632271975153155E-5	0.0	7.245815604007944	0.0
88	0.0	2.6632271975153155E-5	0.0	7.710388956342515	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	4100	0.0	40.37361	94
GTATCAA	14050	0.0	34.421917	1
GTACTAG	4845	0.0	34.373814	1
GGTATCA	9025	0.0	32.99702	1
TAGGCAT	6050	0.0	30.995724	5
CAACGCA	15335	0.0	29.483263	5
CTGTGCG	6040	0.0	29.10445	9
CAGTACG	520	0.0	28.924866	9
TAGTACT	6175	0.0	28.79641	4
CTAGGCA	6780	0.0	28.724611	4
ATCAACG	15845	0.0	28.560535	3
ACTGTGC	6505	0.0	27.888815	8
CTAACGC	2215	0.0	27.396778	3
TATCAAC	17735	0.0	27.216633	2
TCTAACG	2250	0.0	27.181854	2
TAACGCC	2255	0.0	26.910803	4
GTACTGT	6735	0.0	26.586786	6
GCATCGT	5730	0.0	26.575436	8
AACGCAG	18210	0.0	26.479876	6
CCGTGTT	555	0.0	26.253876	9
>>END_MODULE