FastQCFastQC Report
Mon 16 Jan 2017
ERR1146643_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146643_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6029575
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC92580.1535431601729807No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC87020.1443219464058412No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC72050.043.8276494
GTACTAG77300.036.255261
TCTAACG37000.033.787872
CTAACGC37500.033.713073
GCATCGT77650.033.4057248
TAACGCC39050.032.3746384
CGCCTAC39150.032.1681677
CAACGCA114100.032.043955
TATTCTC31000.031.8353525
TAGGCAT95600.031.6577515
GTATCAA132350.031.4074441
CTGTGCG105650.031.179439
TAGTACT108150.030.9839444
ACTGTGC113800.029.937748
ATCAACG123200.029.793233
GTGGTAT19350.029.4041521
GGTATCA87850.028.9574151
AACGCCT43700.028.9282995
CTAGGCA107250.028.8776454
GTACTGT118250.028.7325926
Produced by FastQC (version 0.11.5)