##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146643_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6029575
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.52441589995978	31.0	31.0	34.0	28.0	34.0
2	31.63815376705655	33.0	31.0	34.0	28.0	34.0
3	31.768916051297147	33.0	31.0	34.0	28.0	34.0
4	35.17938809949292	37.0	35.0	37.0	32.0	37.0
5	35.35424453630645	37.0	35.0	37.0	32.0	37.0
6	35.40609761052811	37.0	35.0	37.0	32.0	37.0
7	35.31288308048246	37.0	35.0	37.0	32.0	37.0
8	35.32723848695804	37.0	35.0	37.0	32.0	37.0
9	36.838260905619386	39.0	37.0	39.0	32.0	39.0
10-11	36.67585725362069	39.0	37.0	39.0	32.0	39.0
12-13	36.610503808311535	39.0	37.0	39.0	32.0	39.0
14-15	37.820224393924946	40.0	38.0	41.0	32.0	41.0
16-17	37.8663343602161	40.0	38.0	41.0	32.0	41.0
18-19	37.8440628070801	40.0	38.0	41.0	32.0	41.0
20-21	37.908034811740464	40.0	38.0	41.0	32.0	41.0
22-23	37.858550063644614	40.0	38.0	41.0	32.0	41.0
24-25	37.82895544047466	40.0	37.0	41.0	32.0	41.0
26-27	37.86224095396442	40.0	37.0	41.0	32.0	41.0
28-29	37.867892347304746	40.0	37.0	41.0	32.0	41.0
30-31	37.74311456445935	40.0	37.0	41.0	32.0	41.0
32-33	37.74213182521156	40.0	37.0	41.0	32.0	41.0
34-35	37.6874149504733	40.0	37.0	41.0	31.5	41.0
36-37	37.583336387722184	40.0	37.0	41.0	31.0	41.0
38-39	37.472041064254114	40.0	36.0	41.0	31.0	41.0
40-41	37.374822686507756	40.0	36.0	41.0	31.0	41.0
42-43	37.220068246932826	40.0	36.0	41.0	30.5	41.0
44-45	37.05148795727725	39.5	35.5	41.0	30.0	41.0
46-47	37.03215566602953	39.5	35.0	41.0	30.0	41.0
48-49	36.883750927718786	39.0	35.0	41.0	30.0	41.0
50-51	36.69103916942736	39.0	35.0	41.0	30.0	41.0
52-53	36.466713242641475	39.0	35.0	41.0	30.0	41.0
54-55	36.244861204977134	38.0	35.0	40.5	29.5	41.0
56-57	35.99079155661883	38.0	35.0	40.0	29.0	41.0
58-59	35.67402403983697	37.0	34.0	40.0	29.0	41.0
60-61	35.4260681723007	37.0	34.0	40.0	28.0	41.0
62-63	35.10709701761733	36.0	34.0	40.0	28.0	41.0
64-65	34.943710045898754	36.0	34.0	39.0	28.0	41.0
66-67	34.693476240033505	35.0	34.0	39.0	28.0	41.0
68-69	34.558002512614905	35.0	34.0	39.0	28.0	41.0
70-71	34.4871008321482	35.0	34.0	38.5	28.0	41.0
72-73	34.27442838010971	35.0	34.0	37.5	28.0	40.5
74-75	34.02416289705327	35.0	33.5	37.0	28.0	40.0
76-77	33.77028613127791	35.0	33.0	36.5	27.5	39.0
78-79	33.52472346724272	35.0	33.0	36.0	27.0	39.0
80-81	33.35584498078223	35.0	33.0	36.0	27.0	39.0
82-83	33.09099198533893	35.0	33.0	35.5	26.5	37.0
84-85	32.924234958516976	35.0	33.0	35.0	26.5	37.0
86-87	32.75958902576052	35.0	33.0	35.0	26.0	36.5
88-89	32.63901419254259	35.0	33.0	35.0	26.0	36.0
90-91	32.48744828615615	35.0	33.0	35.0	26.0	36.0
92-93	32.333111703561194	35.0	33.0	35.0	25.0	36.0
94-95	32.27311087431535	35.0	33.0	35.0	25.0	36.0
96-97	32.16234999979269	35.0	33.0	35.0	25.0	35.0
98-99	32.07241032742772	35.0	32.5	35.0	25.0	35.0
100	31.14023857402885	34.0	31.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3456455427941876
1101	2	0.42740394823068684
1101	3	0.504309076665642
1101	4	0.536881033297604
1101	5	0.4262212966439023
1101	6	0.43052197545335247
1101	7	0.4836368812876941
1101	8	0.44300819871197916
1101	9	0.6878807820810451
1101	10-11	0.7036282928244333
1101	12-13	0.5924472120871869
1101	14-15	0.8090290763023944
1101	16-17	0.7527385863792446
1101	18-19	0.760077832723276
1101	20-21	0.7143938482886654
1101	22-23	0.7405505896897253
1101	24-25	0.7689783469816263
1101	26-27	0.7281977345707205
1101	28-29	0.6859064425874593
1101	30-31	0.7284272599774155
1101	32-33	0.7140350665693873
1101	34-35	0.7109674733861624
1101	36-37	0.6838580906884033
1101	38-39	0.691206039025829
1101	40-41	0.6645697903834815
1101	42-43	0.6362215128889233
1101	44-45	0.6102409320457625
1101	46-47	0.6304937901925882
1101	48-49	0.5954267768565842
1101	50-51	0.5421456749724598
1101	52-53	0.6139772642563344
1101	54-55	0.6001256997954201
1101	56-57	0.5687254995063142
1101	58-59	0.5575087979526998
1101	60-61	0.5565634526825036
1101	62-63	0.6035633728579555
1101	64-65	0.5788717382738398
1101	66-67	0.599077506760338
1101	68-69	0.5964955625968003
1101	70-71	0.588327454409594
1101	72-73	0.5594816046602347
1101	74-75	0.5096522474375007
1101	76-77	0.5205157780856808
1101	78-79	0.5540786222751137
1101	80-81	0.49165705302598184
1101	82-83	0.4755280972773903
1101	84-85	0.48492322596069926
1101	86-87	0.5056413300631775
1101	88-89	0.5244164449567208
1101	90-91	0.5010281613670955
1101	92-93	0.5293532147793627
1101	94-95	0.5713212944964567
1101	96-97	0.4955474812153966
1101	98-99	0.5048130061132952
1101	100	0.5792160879207948
1102	1	0.3262669244560641
1102	2	0.38285578012716925
1102	3	0.43295854614378015
1102	4	0.5243588817180367
1102	5	0.36855375562293347
1102	6	0.37904611386775855
1102	7	0.36714467690519115
1102	8	0.42965416835982495
1102	9	0.5177245132546346
1102	10-11	0.641304738351252
1102	12-13	0.5482091304500258
1102	14-15	0.7162420509788063
1102	16-17	0.6900843177966678
1102	18-19	0.74566326456452
1102	20-21	0.6548247662641629
1102	22-23	0.7144364734826993
1102	24-25	0.7124047908494546
1102	26-27	0.7106581573286732
1102	28-29	0.6222005206866115
1102	30-31	0.7196403022198083
1102	32-33	0.6869829444572488
1102	34-35	0.7329507961047241
1102	36-37	0.8174749406579664
1102	38-39	0.7583215478489791
1102	40-41	0.6903419017772023
1102	42-43	0.7432497834404614
1102	44-45	0.7277430340224669
1102	46-47	0.661289421623863
1102	48-49	0.6384191517718989
1102	50-51	0.6424729984430257
1102	52-53	0.5902072476275322
1102	54-55	0.6161732999772127
1102	56-57	0.574068552634408
1102	58-59	0.5771134977263657
1102	60-61	0.5855642049573575
1102	62-63	0.5688426827986461
1102	64-65	0.6247927860489639
1102	66-67	0.6193370879269153
1102	68-69	0.5594950141167487
1102	70-71	0.5099406079384323
1102	72-73	0.5637780774032066
1102	74-75	0.528361190612813
1102	76-77	0.5138031084159493
1102	78-79	0.5158068173139867
1102	80-81	0.5335042132830168
1102	82-83	0.547550352701272
1102	84-85	0.5641029650276579
1102	86-87	0.4913102733525534
1102	88-89	0.5288111543645755
1102	90-91	0.4801485786506703
1102	92-93	0.49646681508279045
1102	94-95	0.528429754410439
1102	96-97	0.543785570640182
1102	98-99	0.47276250240393836
1102	100	0.6048788638945268
1103	1	0.23516447099961368
1103	2	0.30657517952563396
1103	3	0.33265068236706696
1103	4	0.45860792103638204
1103	5	0.30374589952604936
1103	6	0.3415152782742865
1103	7	0.3076489906973663
1103	8	0.37441754202415467
1103	9	0.38801880644972186
1103	10-11	0.5215699909775964
1103	12-13	0.4723576537920593
1103	14-15	0.5902032459947435
1103	16-17	0.5361951822879334
1103	18-19	0.5810976529765028
1103	20-21	0.5441559483801583
1103	22-23	0.5941804442223386
1103	24-25	0.5501210077702154
1103	26-27	0.5242616865019585
1103	28-29	0.5436868747128543
1103	30-31	0.5488521095127936
1103	32-33	0.5032439106575666
1103	34-35	0.4894660760431293
1103	36-37	0.45409189828197327
1103	38-39	0.5027435044572997
1103	40-41	0.4308760803676108
1103	42-43	0.46409528822044877
1103	44-45	0.427008923533748
1103	46-47	0.4175362050196014
1103	48-49	0.4661049176314549
1103	50-51	0.3833762955556921
1103	52-53	0.42269890960517387
1103	54-55	0.40793939879086594
1103	56-57	0.43948769604579496
1103	58-59	0.42677163177896915
1103	60-61	0.4117892620098331
1103	62-63	0.387254028047181
1103	64-65	0.38017188402173474
1103	66-67	0.362608611620054
1103	68-69	0.3273070244600689
1103	70-71	0.32783556583891027
1103	72-73	0.3415048816707653
1103	74-75	0.33740003518583705
1103	76-77	0.29682499224978187
1103	78-79	0.2961623944595857
1103	80-81	0.29440435084929817
1103	82-83	0.3453859498533731
1103	84-85	0.3890200174705072
1103	86-87	0.39540627748365154
1103	88-89	0.4714510824640712
1103	90-91	0.4255663061612651
1103	92-93	0.4026122517827275
1103	94-95	0.3722009212619639
1103	96-97	0.36775394791835936
1103	98-99	0.4203562714885791
1103	100	0.5047175117774145
1104	1	0.2500712726909846
1104	2	0.3108962035030878
1104	3	0.33988030655223866
1104	4	0.4332557717515826
1104	5	0.31284903840999334
1104	6	0.31660445501082535
1104	7	0.3417656581642561
1104	8	0.3669267322549885
1104	9	0.39407047823601715
1104	10-11	0.5696477679651792
1104	12-13	0.3781933917054161
1104	14-15	0.5481852633582633
1104	16-17	0.5056070472252046
1104	18-19	0.4993124504123756
1104	20-21	0.440740445977859
1104	22-23	0.4775630213569215
1104	24-25	0.5591699510967345
1104	26-27	0.47314904854922446
1104	28-29	0.45550822088537757
1104	30-31	0.46528937389072667
1104	32-33	0.5009069133856627
1104	34-35	0.5017535762100991
1104	36-37	0.476795910937291
1104	38-39	0.46596552375579137
1104	40-41	0.5112340129185498
1104	42-43	0.5312768412620628
1104	44-45	0.5216414514816918
1104	46-47	0.5545179808721912
1104	48-49	0.472008487986038
1104	50-51	0.407085836609113
1104	52-53	0.5124795137146094
1104	54-55	0.5299220464654084
1104	56-57	0.5293855358406532
1104	58-59	0.49408134084313815
1104	60-61	0.5127417320095873
1104	62-63	0.49675906241284906
1104	64-65	0.515439934075502
1104	66-67	0.448947730144873
1104	68-69	0.45844845058751105
1104	70-71	0.44892432525611525
1104	72-73	0.4317571811688552
1104	74-75	0.3694807817831176
1104	76-77	0.33449451468990077
1104	78-79	0.3063572060539883
1104	80-81	0.34399148088000686
1104	82-83	0.3593560393207227
1104	84-85	0.4132309463995085
1104	86-87	0.39544725131057845
1104	88-89	0.28287919722400545
1104	90-91	0.3012624099654815
1104	92-93	0.35812221167169866
1104	94-95	0.41062854975101004
1104	96-97	0.36397176586532964
1104	98-99	0.3568334826402477
1104	100	0.40395547079709004
1105	1	0.18063742737408006
1105	2	0.2844956442442026
1105	3	0.26959573767551603
1105	4	0.34614346072390845
1105	5	0.22096569010270883
1105	6	0.22358970355688967
1105	7	0.2191173752369835
1105	8	0.30784740630605256
1105	9	0.3896790639653176
1105	10-11	0.45618926850721664
1105	12-13	0.286132643772838
1105	14-15	0.402129207702032
1105	16-17	0.3676702358090651
1105	18-19	0.4460956570062464
1105	20-21	0.3675918330516552
1105	22-23	0.3868157174655593
1105	24-25	0.3757226874953332
1105	26-27	0.42260007078174766
1105	28-29	0.47231701118683844
1105	30-31	0.4864708786014731
1105	32-33	0.4429905828594016
1105	34-35	0.4840274261457651
1105	36-37	0.4721626877098615
1105	38-39	0.525512082531236
1105	40-41	0.46373587270797145
1105	42-43	0.5353851849890248
1105	44-45	0.46172241912726264
1105	46-47	0.4966566189137609
1105	48-49	0.4118911468538755
1105	50-51	0.425604870173089
1105	52-53	0.44916222253000626
1105	54-55	0.4206596001752061
1105	56-57	0.4530369435610666
1105	58-59	0.4277183397665496
1105	60-61	0.448132111042824
1105	62-63	0.43643385453555794
1105	64-65	0.45530522920490313
1105	66-67	0.401485471868277
1105	68-69	0.40552585622300796
1105	70-71	0.45401646570261533
1105	72-73	0.4055273995222919
1105	74-75	0.37432912666899654
1105	76-77	0.3999678629746839
1105	78-79	0.4826541489765077
1105	80-81	0.43504370795790237
1105	82-83	0.48396686420459645
1105	84-85	0.4218532116370781
1105	86-87	0.4182735468388543
1105	88-89	0.47507854681809647
1105	90-91	0.44775129914190614
1105	92-93	0.4817314600242355
1105	94-95	0.4669539504519449
1105	96-97	0.35859878760690833
1105	98-99	0.40064340775548146
1105	100	0.5028921536641313
1106	1	0.11031760985933303
1106	2	0.20493448381343882
1106	3	0.17955475388170683
1106	4	0.25871212875821925
1106	5	0.14433410562445914
1106	6	0.18545191135127226
1106	7	0.15619560082603812
1106	8	0.19635066018474134
1106	9	0.30921124560988034
1106	10-11	0.3111985321239388
1106	12-13	0.2474592332845802
1106	14-15	0.3748547480225639
1106	16-17	0.29604188008008947
1106	18-19	0.3083053551334132
1106	20-21	0.30991872754313476
1106	22-23	0.3600642161732921
1106	24-25	0.37402097861885863
1106	26-27	0.3409320372271267
1106	28-29	0.3487494831142257
1106	30-31	0.4259165522297508
1106	32-33	0.33909096383658976
1106	34-35	0.3214476688045451
1106	36-37	0.37133885215371976
1106	38-39	0.39673115240776724
1106	40-41	0.37199931961103516
1106	42-43	0.32273644549579217
1106	44-45	0.3181649161688398
1106	46-47	0.4110918600267013
1106	48-49	0.31470369968275946
1106	50-51	0.38059537239590213
1106	52-53	0.33635479648283706
1106	54-55	0.45761578627605104
1106	56-57	0.34012109931166634
1106	58-59	0.37591785514955944
1106	60-61	0.31911663969874127
1106	62-63	0.33262314034230656
1106	64-65	0.24346686580003762
1106	66-67	0.3082995511188429
1106	68-69	0.3518017814043617
1106	70-71	0.33111077411822976
1106	72-73	0.22294705392427971
1106	74-75	0.255557890408312
1106	76-77	0.25270477054868934
1106	78-79	0.27885843628341433
1106	80-81	0.20091935960936524
1106	82-83	0.21278267091216918
1106	84-85	0.25480527270612896
1106	86-87	0.18287435653910933
1106	88-89	0.23820976158931728
1106	90-91	0.1997387123502321
1106	92-93	0.25521128262577975
1106	94-95	0.2958384251938355
1106	96-97	0.32010846966649353
1106	98-99	0.28640793598495407
1106	100	0.26796818537897593
1107	1	0.10701439803588642
1107	2	0.17347099049669978
1107	3	0.10810309112423866
1107	4	0.2527916036169984
1107	5	0.16477751894917247
1107	6	0.20633366443053092
1107	7	0.178137771840035
1107	8	0.16234484486113843
1107	9	0.17622156254919474
1107	10-11	0.27508422972672264
1107	12-13	0.19508712795404648
1107	14-15	0.30557905966158216
1107	16-17	0.24287311349482366
1107	18-19	0.25375966745435363
1107	20-21	0.27589457723257027
1107	22-23	0.32721690489348987
1107	24-25	0.35792748631043025
1107	26-27	0.27973879967544235
1107	28-29	0.24064409292919464
1107	30-31	0.34708326014811774
1107	32-33	0.272544760058004
1107	34-35	0.25301690764079154
1107	36-37	0.3026910338860276
1107	38-39	0.3014111493303986
1107	40-41	0.22371321833740865
1107	42-43	0.2831020805915614
1107	44-45	0.3301887213445269
1107	46-47	0.29689748138314087
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2216	84-85	-1.1474157444963033
2216	86-87	-1.111550213386984
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2216	90-91	-1.2205841795040016
2216	92-93	-1.1712485795957264
2216	94-95	-1.1515081868391697
2216	96-97	-1.194333800555473
2216	98-99	-1.1907498715054388
2216	100	-0.8379032925929906
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	3.0
2	11.0
3	27.0
4	48.0
5	48.0
6	67.0
7	242.0
8	663.0
9	1009.0
10	1319.0
11	2001.0
12	3457.0
13	5139.0
14	7478.0
15	10713.0
16	13547.0
17	16248.0
18	19314.0
19	22027.0
20	26063.0
21	30657.0
22	35473.0
23	42030.0
24	50041.0
25	57950.0
26	64793.0
27	71169.0
28	81847.0
29	100179.0
30	117497.0
31	136764.0
32	170041.0
33	221976.0
34	329420.0
35	483077.0
36	783604.0
37	1429391.0
38	1188902.0
39	504423.0
40	917.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.803927129611175	21.26082228362776	11.927724379372497	24.00752620738857
2	15.669116229912847	20.012648649832098	40.40885359046836	23.909381529786693
3	18.256504500260817	27.305789674901458	29.092459150170964	25.34524667466676
4	11.823372503019582	16.655382285102192	41.136944844088106	30.384300367790118
5	12.613611334463807	39.204966125578174	35.496459970331685	12.684962569626332
6	29.087173169443716	39.39210445309536	18.323425255878504	13.197297121582427
7	26.327897460375322	32.18341182778775	24.329311504925855	17.159379206911073
8	26.296452073756104	34.89884889179755	19.998460899040616	18.806238135405724
9	26.312719502503008	14.637790187782029	21.716982246086936	37.332508063628026
10-11	25.705635222001245	25.826257832363765	27.794663598001808	20.673443347633185
12-13	25.682379582463867	23.965762355540917	27.715844036432497	22.63601402556272
14-15	23.101559365705697	25.124880443322052	25.082083008614052	26.691477182358202
16-17	21.85728125897404	27.579720159624	26.499240531353568	24.063758050048392
18-19	21.072646842118576	28.654680277022344	28.16959678512773	22.103076095731346
20-21	23.12710506116909	27.702339623943285	26.91585858632766	22.25469672855997
22-23	23.75527780256333	27.288789215951308	26.558322102437053	22.397610879048305
24-25	23.12768829676599	27.25456201406039	27.621367407185165	21.996382281988456
26-27	22.97932411884278	27.566663968750166	26.275545067589444	23.17846684481761
28-29	21.89716434831493	28.232390073208098	26.99144571787911	22.87899986059787
30-31	22.891536390607918	27.78878891695946	26.982908979693654	22.336765712738966
32-33	23.43859078797549	27.972205198546735	25.870445176255163	22.718758837222612
34-35	23.239890734852896	27.661297946551628	27.18332097715018	21.91549034144529
36-37	22.7766776907577	27.232523564792803	26.73628604485088	23.25451269959862
38-39	23.066009496639307	27.375530243678853	27.40417424542668	22.15428601425516
40-41	22.586982332726315	26.290932368539533	28.189683919015106	22.932401379719046
42-43	22.525073465452824	26.666566036634197	27.327879517320863	23.480480980592116
44-45	22.758370605974097	27.803377288591015	27.151222884889954	22.287029220544937
46-47	22.663972208318178	27.490666090507094	26.62101901451002	23.224342686664713
48-49	23.53405900623797	27.073207388420244	26.820969755039776	22.571763850302013
50-51	22.979909802498575	26.7554818309056	26.880099528277434	23.38450883831839
52-53	22.32210354172988	27.571593301507637	26.65428896243773	23.452014194324757
54-55	22.358717447501963	27.41990599188908	26.667212415334312	23.554164145274644
56-57	23.718103534359123	26.705374639678624	26.392476851352694	23.184044974609556
58-59	22.402992979853884	27.249151406807687	27.4684677317286	22.879387881609826
60-61	22.417563642576457	28.022157751646738	26.54806513629223	23.01221346948457
62-63	22.952524444210752	27.149463793574313	26.057876572959675	23.840135189255253
64-65	21.905131554394476	27.521768157409138	26.641023408181457	23.932076880014925
66-67	22.867939506913736	26.92865917519501	26.56150839265768	23.641892925233574
68-69	22.139197552338672	27.10585559717017	26.938679346597027	23.81626750389413
70-71	22.171659492079538	26.747823386978464	27.045482987367215	24.035034133574786
72-73	22.989061294897205	26.781615677428448	27.590665196009322	22.63865783166502
74-75	23.166442992873122	27.139559675289014	26.954035657434254	22.73996167440361
76-77	22.788685435259172	27.108290299779252	26.59483179365722	23.50819247130435
78-79	22.63419550372743	27.049083932898267	26.65777160888639	23.65894895448791
80-81	22.096110205710172	27.548757091520265	27.413177781480286	22.941954921289277
82-83	23.12313423026562	26.296326673251862	28.633427047009125	21.94711204947339
84-85	23.446876054036675	27.02221821191259	26.90680754574773	22.624098188303
86-87	21.834687222460747	27.8479420141634	27.48609033072624	22.831280432649617
88-89	23.2782862393599	26.35287639241808	27.111698633954102	23.257138734267915
90-91	22.776904141406845	26.449035419469723	27.391078149480315	23.38298228964312
92-93	23.169533338657576	26.691327352105343	27.290063848584584	22.8490754606525
94-95	21.773778894725737	26.915857483868315	27.991610063677534	23.318753557728414
96-97	22.30039348157132	26.734039929348143	27.41416941684681	23.55139717223373
98-99	21.50272955306585	27.67066693713677	27.206477880527295	23.620125629270085
100	22.491133585646644	26.901716376845602	26.15990925634202	24.447240781165736
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	552.0
1	386.0
2	278.0
3	336.5
4	320.0
5	305.5
6	281.5
7	281.5
8	308.0
9	327.0
10	312.0
11	276.0
12	305.5
13	349.5
14	383.5
15	702.5
16	924.5
17	1021.5
18	1453.0
19	1935.0
20	2389.0
21	2954.5
22	4024.5
23	5698.0
24	7530.0
25	9375.5
26	12851.0
27	16882.5
28	22956.5
29	36581.5
30	55612.0
31	72935.0
32	95789.0
33	116967.5
34	130383.5
35	147929.5
36	163461.5
37	191178.5
38	200204.0
39	182008.0
40	166705.0
41	149973.5
42	146238.5
43	157718.5
44	175548.0
45	215542.5
46	252529.5
47	277396.5
48	293134.5
49	283551.5
50	267546.5
51	278802.0
52	287971.5
53	273602.5
54	256377.5
55	216236.0
56	186707.5
57	151176.5
58	113999.5
59	112387.0
60	92783.5
61	59606.0
62	42625.0
63	36321.5
64	26106.5
65	10073.0
66	3975.5
67	2414.0
68	1539.5
69	933.0
70	509.5
71	283.5
72	161.0
73	91.0
74	68.5
75	53.0
76	44.0
77	40.5
78	31.5
79	27.0
80	29.0
81	22.0
82	17.5
83	22.5
84	21.5
85	14.5
86	10.0
87	11.0
88	12.5
89	11.0
90	10.0
91	7.0
92	8.0
93	9.0
94	5.5
95	5.0
96	5.5
97	3.5
98	5.0
99	7.5
100	17.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06133102250158593
2	0.007844665668807503
3	0.007960760086739115
4	0.009834855690492281
5	0.004096474461301169
6	0.0025043224439533465
7	0.0010282648445371356
8	0.0013433782646372257
9	5.141324222685678E-4
10-11	4.2291537960801543E-4
12-13	2.1560391901585103E-4
14-15	1.3267933477898526E-4
16-17	5.058399638448813E-4
18-19	0.0016170293926188828
20-21	9.785100939950161E-4
22-23	0.0012770385972477331
24-25	0.0013848405567556585
26-27	0.003831115791743199
28-29	0.004237446254503841
30-31	0.005423267809091022
32-33	0.006468117570475531
34-35	0.003399907953711497
36-37	0.008400260383194504
38-39	0.006459825112051844
40-41	0.006244221193035994
42-43	0.014777160911009482
44-45	0.0206979762255217
46-47	0.019114116666597562
48-49	0.02207452432385367
50-51	0.018865342913886966
52-53	0.025408092610175674
54-55	0.019221918626105488
56-57	0.01540738775120966
58-59	0.017712691192994533
60-61	0.01633614309466256
62-63	0.011501639833653285
64-65	0.011609441793161208
66-67	0.014146934070809303
68-69	0.005133031764261992
70-71	0.008964147556005192
72-73	0.008707081344870908
74-75	0.011708951294245448
76-77	0.010871412993453105
78-79	0.012587951887156225
80-81	0.010473374989116149
82-83	0.0065427496962887095
84-85	0.011103601829316329
86-87	0.009710468814136983
88-89	0.008209533839449713
90-91	0.013508414772185435
92-93	0.009047072140242057
94-95	0.007413457830775801
96-97	0.009552912104086939
98-99	0.009387062935613207
100	0.26787957691877123
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6029575.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.79074327498382
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.74680996264419	43.007511619631714
2	16.09341216121228	19.566609742215164
3	6.237788749376795	11.375994434008415
4	2.864349806587744	6.965038149681003
5	1.4993626641789861	4.557368539710026
6	0.8701092872839391	3.173675418267425
7	0.5156333055363389	2.194201233062361
8	0.3334207179749024	1.6215114615178459
9	0.2307495553513841	1.2624693282164396
>10	0.6037371952712615	5.080821766894518
>50	0.0026603971174241265	0.10720800625123081
>100	0.0012736157889742896	0.14288796726509626
>500	3.4629086447484833E-4	0.1478326017199328
>1k	2.597181084532291E-4	0.324268589703751
>5k	8.657270281774294E-5	0.4726011418550162
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	9258	0.1535431601729807	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	8702	0.1443219464058412	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	1.6584916847373158E-5	0.0
18-19	0.0	0.0	0.0	1.6584916847373158E-5	0.0
20-21	0.0	0.0	0.0	3.3169833694746316E-5	0.0
22-23	0.0	0.0	0.0	7.46321258131792E-5	0.0
24-25	0.0	0.0	0.0	2.0731146059216446E-4	0.0
26-27	0.0	0.0	0.0	6.219343817764933E-4	0.0
28-29	0.0	0.0	0.0	0.0031843040346956463	0.0
30-31	0.0	0.0	0.0	0.011567979501042776	0.0
32-33	0.0	0.0	0.0	0.02598027224141005	0.0
34-35	0.0	0.0	0.0	0.04856892898753229	0.0
36-37	0.0	0.0	0.0	0.08831468221226206	0.0
38-39	0.0	0.0	0.0	0.14600531546584958	0.0
40-41	0.0	0.0	0.0	0.20998992466301522	0.0
42-43	0.0	0.0	0.0	0.2774076116475871	0.0
44-45	0.0	0.0	0.0	0.3582259114448365	0.0
46-47	0.0	0.0	0.0	0.45840710166139403	0.0
48-49	0.0	0.0	0.0	0.5777687482119387	0.0
50-51	0.0	0.0	0.0	0.7091378745599815	0.0
52-53	0.0	0.0	0.0	0.8366675926578573	0.0
54-55	0.0	8.292458423686579E-6	0.0	0.9778218199458503	0.0
56-57	0.0	1.6584916847373158E-5	0.0	1.149384492273502	0.0
58-59	0.0	1.6584916847373158E-5	0.0	1.3608670594527807	0.0
60-61	0.0	1.6584916847373158E-5	0.0	1.57979625429653	0.0
62-63	0.0	1.6584916847373158E-5	0.0	1.811147551858962	0.0
64-65	0.0	1.6584916847373158E-5	0.0	2.050567411467641	0.0
66-67	0.0	1.6584916847373158E-5	0.0	2.326830663852759	0.0
68-69	0.0	1.6584916847373158E-5	0.0	2.63985438443008	0.0
70-71	0.0	1.6584916847373158E-5	0.0	2.9907000078778356	0.0
72-73	0.0	1.6584916847373158E-5	0.0	3.350642458216375	0.0
74-75	0.0	1.6584916847373158E-5	0.0	3.698859372343822	0.0
76-77	0.0	1.6584916847373158E-5	0.0	4.1042196174688925	0.0
78-79	0.0	1.6584916847373158E-5	0.0	4.541721431444174	0.0
80-81	0.0	1.6584916847373158E-5	0.0	5.02375374715465	0.0
82-83	0.0	1.6584916847373158E-5	0.0	5.529336644788398	0.0
84-85	0.0	1.6584916847373158E-5	0.0	6.0171405115617596	0.0
86-87	0.0	1.6584916847373158E-5	0.0	6.534996247662564	0.0
88	0.0	1.6584916847373158E-5	0.0	6.95692482471816	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	7205	0.0	43.82764	94
GTACTAG	7730	0.0	36.25526	1
TCTAACG	3700	0.0	33.78787	2
CTAACGC	3750	0.0	33.71307	3
GCATCGT	7765	0.0	33.405724	8
TAACGCC	3905	0.0	32.374638	4
CGCCTAC	3915	0.0	32.168167	7
CAACGCA	11410	0.0	32.04395	5
TATTCTC	3100	0.0	31.835352	5
TAGGCAT	9560	0.0	31.657751	5
GTATCAA	13235	0.0	31.407444	1
CTGTGCG	10565	0.0	31.17943	9
TAGTACT	10815	0.0	30.983944	4
ACTGTGC	11380	0.0	29.93774	8
ATCAACG	12320	0.0	29.79323	3
GTGGTAT	1935	0.0	29.404152	1
GGTATCA	8785	0.0	28.957415	1
AACGCCT	4370	0.0	28.928299	5
CTAGGCA	10725	0.0	28.877645	4
GTACTGT	11825	0.0	28.732592	6
>>END_MODULE