FastQCFastQC Report
Mon 16 Jan 2017
ERR1146643_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146643_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6029575
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172090.28540983402644465No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143000.23716431091743614No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90390.14991106338340596No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC65750.044.9905594
GGTATCA128950.044.1199041
GTGGTAT23150.041.6708561
GTATCAA226200.040.0259861
CAACGCA261850.033.0269625
GTACTAG73750.032.669131
ATCAACG265700.032.5851553
CTGTGCG99700.031.3528299
TATCAAC296700.030.4835412
ACTGTGC107150.030.439058
TAGTACT103550.030.399724
AACGCAG306150.029.2124716
TAGGCAT94600.029.0648565
GAACCGT6250.028.573766
CTAACGC35450.028.4047553
TACTGTG122200.028.382397
GTACTGT111150.028.3711436
TATTCGC6850.028.1347799
TCTAACG35800.028.129392
CGCCTAC36750.027.3665627
Produced by FastQC (version 0.11.5)