FastQCFastQC Report
Mon 16 Jan 2017
ERR1146642_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146642_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4854524
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC51250.039.9894894
GTACTAG54400.036.043161
GCATCGT53450.033.6740348
CTGTGCG67400.031.9330759
CAACGCA81950.031.8844435
TAGGCAT65300.031.8818555
TAGTACT70550.030.8435134
GTATCAA97900.029.9700581
GGTATCA63650.029.6970521
ACTGTGC77900.029.3789028
ATCAACG89900.029.2234823
CTAGGCA73350.029.1535284
TCTAACG22600.029.113842
CTAACGC23350.028.5812573
CTAGTAC81150.027.799223
GTACTGT81800.027.518886
TAACGCC24600.027.1289564
TACTGTG86700.026.9931967
CATCGTC69700.026.092369
GGCGTCG34050.026.0848778
Produced by FastQC (version 0.11.5)