##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146642_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4854524
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.364916313113294	31.0	31.0	34.0	28.0	34.0
2	31.380995541478423	31.0	31.0	34.0	28.0	34.0
3	31.547249122674025	33.0	31.0	34.0	28.0	34.0
4	34.98472496994556	37.0	35.0	37.0	32.0	37.0
5	35.158948230557726	37.0	35.0	37.0	32.0	37.0
6	35.208620041841385	37.0	35.0	37.0	32.0	37.0
7	35.14783879943739	37.0	35.0	37.0	32.0	37.0
8	35.14079794435047	37.0	35.0	37.0	32.0	37.0
9	36.624295193514335	39.0	37.0	39.0	32.0	39.0
10-11	36.458262540261416	39.0	37.0	39.0	32.0	39.0
12-13	36.38021153052287	39.0	37.0	39.0	31.5	39.0
14-15	37.55689394057997	40.0	37.5	41.0	31.0	41.0
16-17	37.580065831377084	40.0	37.5	41.0	31.0	41.0
18-19	37.551112220271236	40.0	37.0	41.0	31.0	41.0
20-21	37.63096896832728	40.0	37.0	41.0	31.0	41.0
22-23	37.57100273888851	40.0	37.0	41.0	31.0	41.0
24-25	37.54391645813266	40.0	37.0	41.0	31.0	41.0
26-27	37.598973555388746	40.0	37.0	41.0	31.0	41.0
28-29	37.59825422636699	40.0	37.0	41.0	31.0	41.0
30-31	37.47317996573918	40.0	37.0	41.0	31.0	41.0
32-33	37.47901411137323	40.0	37.0	41.0	31.0	41.0
34-35	37.417016786815765	40.0	36.5	41.0	30.5	41.0
36-37	37.296132535342295	40.0	36.0	41.0	30.0	41.0
38-39	37.190876695634834	40.0	36.0	41.0	30.0	41.0
40-41	37.10955554035782	40.0	36.0	41.0	30.0	41.0
42-43	36.9387283902603	40.0	35.5	41.0	30.0	41.0
44-45	36.77539888565799	39.0	35.0	41.0	30.0	41.0
46-47	36.77987749159341	39.0	35.0	41.0	30.0	41.0
48-49	36.652850619339816	39.0	35.0	41.0	30.0	41.0
50-51	36.48213326373502	39.0	35.0	41.0	29.0	41.0
52-53	36.27843388970783	39.0	35.0	40.5	29.0	41.0
54-55	36.066918816345336	38.0	35.0	40.0	29.0	41.0
56-57	35.84084783595673	38.0	34.0	40.0	28.0	41.0
58-59	35.56139170390341	37.5	34.0	40.0	28.0	41.0
60-61	35.3223201698045	37.0	34.0	40.0	28.0	41.0
62-63	35.021990827525	36.5	34.0	40.0	27.5	41.0
64-65	34.866043303112725	36.0	33.5	39.5	28.0	41.0
66-67	34.610039006913965	35.5	33.0	39.0	27.5	41.0
68-69	34.47969831851691	35.0	33.0	39.0	27.0	41.0
70-71	34.38089275076197	35.0	33.0	39.0	27.5	41.0
72-73	34.14997598116726	35.0	33.0	38.0	27.0	40.5
74-75	33.88820314823863	35.0	33.0	37.0	27.0	40.0
76-77	33.61254316592111	35.0	33.0	37.0	26.5	39.0
78-79	33.35278309469682	35.0	33.0	36.0	26.0	39.0
80-81	33.158640476388626	35.0	33.0	36.0	26.0	39.0
82-83	32.89946264556525	35.0	33.0	36.0	26.0	37.0
84-85	32.715178254345844	35.0	33.0	35.0	26.0	37.0
86-87	32.539180669412694	35.0	33.0	35.0	26.0	36.5
88-89	32.40553512558595	35.0	32.0	35.0	25.0	36.0
90-91	32.25843738747609	35.0	32.0	35.0	25.0	36.0
92-93	32.1109104620762	35.0	32.0	35.0	25.0	36.0
94-95	32.038571649867215	35.0	32.0	35.0	25.0	35.5
96-97	31.935378731261807	35.0	32.0	35.0	25.0	35.0
98-99	31.846841008510825	35.0	32.0	35.0	25.0	35.0
100	30.888378963622387	34.0	30.0	35.0	21.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.408853478221225
1101	2	0.4059279031560621
1101	3	0.5109954308973172
1101	4	0.5806224151023684
1101	5	0.4993309699870494
1101	6	0.4557564514986794
1101	7	0.42134410836472824
1101	8	0.47621547857912105
1101	9	0.6366894588529703
1101	10-11	0.6134646260918188
1101	12-13	0.569788360668376
1101	14-15	0.7307638434644872
1101	16-17	0.7032990926587459
1101	18-19	0.7132993813605353
1101	20-21	0.645768059370802
1101	22-23	0.6734060508028819
1101	24-25	0.7462765482183613
1101	26-27	0.6765097951795127
1101	28-29	0.6751082134051671
1101	30-31	0.7392711559588108
1101	32-33	0.7077207175596811
1101	34-35	0.7136027714420479
1101	36-37	0.7135682447874103
1101	38-39	0.6311778710850646
1101	40-41	0.6260507234824715
1101	42-43	0.6389255735468637
1101	44-45	0.6356505628438285
1101	46-47	0.6170080684634414
1101	48-49	0.5775106352486503
1101	50-51	0.5849816339720846
1101	52-53	0.5786587661377354
1101	54-55	0.6061210724231145
1101	56-57	0.5319607409213205
1101	58-59	0.5050418764398472
1101	60-61	0.5538073102075742
1101	62-63	0.5295228165443362
1101	64-65	0.5519354935729126
1101	66-67	0.5171219085474945
1101	68-69	0.5207720620748404
1101	70-71	0.49676977061457706
1101	72-73	0.5068162405669057
1101	74-75	0.48343160751667824
1101	76-77	0.4561792581463493
1101	78-79	0.45666011813573704
1101	80-81	0.4632315235783011
1101	82-83	0.45242320578568496
1101	84-85	0.45751939557913346
1101	86-87	0.45426555636262833
1101	88-89	0.4702611707786488
1101	90-91	0.4336714724861821
1101	92-93	0.47273542971903737
1101	94-95	0.4831411327605153
1101	96-97	0.5044197723404906
1101	98-99	0.47977160751568704
1101	100	0.5379985219133339
1102	1	0.34531927508446714
1102	2	0.40104710590887294
1102	3	0.41912531034647316
1102	4	0.5081707611033224
1102	5	0.43109942441078175
1102	6	0.40910302503046125
1102	7	0.3878214006876135
1102	8	0.4077919609227436
1102	9	0.5998200918997227
1102	10-11	0.6309824737789782
1102	12-13	0.5848726978616625
1102	14-15	0.7420947311148041
1102	16-17	0.6858215829262875
1102	18-19	0.7242667510610374
1102	20-21	0.6350988227870786
1102	22-23	0.7161654165157003
1102	24-25	0.699081560414065
1102	26-27	0.6889209949926283
1102	28-29	0.6762797575599819
1102	30-31	0.6458587136147429
1102	32-33	0.6146211468340255
1102	34-35	0.6924368921308357
1102	36-37	0.6683697094534011
1102	38-39	0.6427161647991113
1102	40-41	0.6204520840357617
1102	42-43	0.6377782569354551
1102	44-45	0.6745541478914561
1102	46-47	0.6040356337487154
1102	48-49	0.5884901425387312
1102	50-51	0.5617089777892161
1102	52-53	0.516463594459438
1102	54-55	0.4876331798116311
1102	56-57	0.48665530143291846
1102	58-59	0.4726274335847407
1102	60-61	0.4371022332462928
1102	62-63	0.5248367637780262
1102	64-65	0.5225430151819097
1102	66-67	0.5286413480309449
1102	68-69	0.4905859148944671
1102	70-71	0.5076162693582305
1102	72-73	0.5302451705974107
1102	74-75	0.5000063911693857
1102	76-77	0.47731405989301834
1102	78-79	0.446638375273146
1102	80-81	0.39455034413995094
1102	82-83	0.4637344198704696
1102	84-85	0.4732605535737733
1102	86-87	0.4336359698733432
1102	88-89	0.43032380417066207
1102	90-91	0.4125907563722109
1102	92-93	0.4278366852259694
1102	94-95	0.44087429214078355
1102	96-97	0.4681608575476943
1102	98-99	0.3075705652073282
1102	100	0.40460982313343763
1103	1	0.2135624594666261
1103	2	0.25988095849467285
1103	3	0.26543543690816307
1103	4	0.363026557844087
1103	5	0.22090736425629842
1103	6	0.27294006397927717
1103	7	0.24881536521361625
1103	8	0.2786327450680659
1103	9	0.379974924200873
1103	10-11	0.50662834034501
1103	12-13	0.3267676241523958
1103	14-15	0.5084400143034884
1103	16-17	0.43030987977459034
1103	18-19	0.4709442040093137
1103	20-21	0.4271000033499348
1103	22-23	0.5103219014602729
1103	24-25	0.48640218516532485
1103	26-27	0.4345834024516435
1103	28-29	0.43379650178015794
1103	30-31	0.4128640239787842
1103	32-33	0.37127773570137634
1103	34-35	0.41105165017871315
1103	36-37	0.38813126901091266
1103	38-39	0.44997693363976765
1103	40-41	0.40766914747059246
1103	42-43	0.400206843907263
1103	44-45	0.3462157593922939
1103	46-47	0.35513538986452886
1103	48-49	0.35650164236323434
1103	50-51	0.3405469366806173
1103	52-53	0.2982922342782359
1103	54-55	0.24923860794278596
1103	56-57	0.2808099067305605
1103	58-59	0.33659565020104054
1103	60-61	0.3067718166189408
1103	62-63	0.2623712525881672
1103	64-65	0.23693209197524823
1103	66-67	0.28644692530485116
1103	68-69	0.2649196975357171
1103	70-71	0.267962569002151
1103	72-73	0.3034815828285673
1103	74-75	0.28741559428222274
1103	76-77	0.23306590601355026
1103	78-79	0.2589360556803868
1103	80-81	0.22355353122591026
1103	82-83	0.2969730840632323
1103	84-85	0.26100881607514026
1103	86-87	0.2587361966238362
1103	88-89	0.3536338298820638
1103	90-91	0.33400654981636535
1103	92-93	0.27760238000673354
1103	94-95	0.25198199337958016
1103	96-97	0.2977631459455452
1103	98-99	0.27606184491071417
1103	100	0.43501828125544506
1104	1	0.15192047896942285
1104	2	0.1874373760579111
1104	3	0.1462238993851237
1104	4	0.251561283659818
1104	5	0.21025659535784058
1104	6	0.2045225054071551
1104	7	0.19492603143303455
1104	8	0.23618630246619432
1104	9	0.25520953907624744
1104	10-11	0.31873030155892224
1104	12-13	0.26063641306568996
1104	14-15	0.3743380124556097
1104	16-17	0.3594426656337504
1104	18-19	0.2870026068373832
1104	20-21	0.27503284252745885
1104	22-23	0.3137200411792378
1104	24-25	0.3004867686282964
1104	26-27	0.29042181486027374
1104	28-29	0.2529619108184278
1104	30-31	0.1885783291079548
1104	32-33	0.28363608562978015
1104	34-35	0.2643815838485537
1104	36-37	0.28031745855234647
1104	38-39	0.25748716986662146
1104	40-41	0.25336777008178757
1104	42-43	0.26456867874813383
1104	44-45	0.235389149025643
1104	46-47	0.26176927411082573
1104	48-49	0.2678208573120955
1104	50-51	0.11312779262843975
1104	52-53	0.1646525983339444
1104	54-55	0.2805365669297899
1104	56-57	0.23254233831911364
1104	58-59	0.19829429518838282
1104	60-61	0.19512928358204817
1104	62-63	0.23518046529871128
1104	64-65	0.2069868256651901
1104	66-67	0.16340563562640398
1104	68-69	0.24240540374562158
1104	70-71	0.22037970366307036
1104	72-73	0.1744292790091606
1104	74-75	0.11340298100214596
1104	76-77	0.15372307613586145
1104	78-79	0.1879551933903727
1104	80-81	0.15410639558139394
1104	82-83	0.15022722370093078
1104	84-85	0.20231309048790536
1104	86-87	0.1690153499737832
1104	88-89	0.1235777673513141
1104	90-91	0.12228523010996639
1104	92-93	0.1750438569583821
1104	94-95	0.2258591349084753
1104	96-97	0.20117299538774347
1104	98-99	0.10629482996558437
1104	100	0.14793035832492052
1105	1	0.15764760492803376
1105	2	0.25174812804198154
1105	3	0.22621205055885696
1105	4	0.3348533668904423
1105	5	0.20778216898668234
1105	6	0.1777086326523758
1105	7	0.2445555711331906
1105	8	0.24425223633519977
1105	9	0.3925419156953325
1105	10-11	0.38736685023337003
1105	12-13	0.26006836691149005
1105	14-15	0.45788574851984265
1105	16-17	0.40215586588330154
1105	18-19	0.3337600887369163
1105	20-21	0.33505723241569285
1105	22-23	0.37645595694996103
1105	24-25	0.3380296709898616
1105	26-27	0.3091321112337795
1105	28-29	0.32996117624039556
1105	30-31	0.35702366963258214
1105	32-33	0.336275111243026
1105	34-35	0.3710534270561112
1105	36-37	0.33037924146688624
1105	38-39	0.3273129232483427
1105	40-41	0.3518004448014693
1105	42-43	0.31526107257121083
1105	44-45	0.3506337164248947
1105	46-47	0.3919841412604299
1105	48-49	0.24621045353663362
1105	50-51	0.3401795994426706
1105	52-53	0.35691242812478663
1105	54-55	0.3353053681128486
1105	56-57	0.33916821110270945
1105	58-59	0.3473859642371693
1105	60-61	0.39840190690760835
1105	62-63	0.3184900916091564
1105	64-65	0.29902035236185043
1105	66-67	0.2541583593718286
1105	68-69	0.27184804156421194
1105	70-71	0.2944892100796892
1105	72-73	0.27402669299931404
1105	74-75	0.2594196477185946
1105	76-77	0.2667312302858562
1105	78-79	0.27038513571167755
1105	80-81	0.22151834592384745
1105	82-83	0.2783165407295982
1105	84-85	0.2230816548349921
1105	86-87	0.21139483639462497
1105	88-89	0.19859766034292647
1105	90-91	0.19467400373360277
1105	92-93	0.19192698490795834
1105	94-95	0.27486061126801076
1105	96-97	0.19124129304838533
1105	98-99	0.15218222957163619
1105	100	0.2639824608327288
1106	1	0.0441480483019987
1106	2	0.1833493775873194
1106	3	0.14242116559547213
1106	4	0.2592472231802958
1106	5	0.14291904855775073
1106	6	0.17314362909061742
1106	7	0.1452766160850345
1106	8	0.16910758833078887
1106	9	0.25983098369312785
1106	10-11	0.25608977176519687
1106	12-13	0.18872831733959572
1106	14-15	0.32004484461884886
1106	16-17	0.15874471554823089
1106	18-19	0.24098138194537455
1106	20-21	0.2501301169486041
1106	22-23	0.26564206117560474
1106	24-25	0.3007853686108035
1106	26-27	0.2979010614670301
1106	28-29	0.3121260084442241
1106	30-31	0.4205359132032669
1106	32-33	0.32826651951805275
1106	34-35	0.25961118433927055
1106	36-37	0.31506138105470427
1106	38-39	0.3431512980485678
1106	40-41	0.3881507816335912
1106	42-43	0.2885382144093498
1106	44-45	0.3270560204793753
1106	46-47	0.3381683926482424
1106	48-49	0.271190266229155
1106	50-51	0.27939223876488484
1106	52-53	0.21282576597573666
1106	54-55	0.26601846246426675
1106	56-57	0.2523477407369299
1106	58-59	0.2671610474577406
1106	60-61	0.31116244361155765
1106	62-63	0.3215044906654043
1106	64-65	0.2997603737712353
1106	66-67	0.3290748927442877
1106	68-69	0.34068017701225983
1106	70-71	0.294184334899974
1106	72-73	0.25656853336862895
1106	74-75	0.2695600950881456
1106	76-77	0.20236925927943616
1106	78-79	0.22980366182800793
1106	80-81	0.21288351383312687
1106	82-83	0.25866514982346445
1106	84-85	0.2552909971056252
1106	86-87	0.23915069416720058
1106	88-89	0.31614017801118877
1106	90-91	0.23647924329065972
1106	92-93	0.2653748975969137
1106	94-95	0.2146477762241048
1106	96-97	0.28107943457114715
1106	98-99	0.2726560401176954
1106	100	0.20261359864402806
1107	1	0.13832966471790442
1107	2	0.1856838562695593
1107	3	0.12864475561940125
1107	4	0.27572402262028106
1107	5	0.13053455271884928
1107	6	0.1960885753760806
1107	7	0.11811368416690016
1107	8	0.14392998642336607
1107	9	0.2129948598655389
1107	10-11	0.28356402777463785
1107	12-13	0.18146796647044283
1107	14-15	0.28544164795264493
1107	16-17	0.12436265369615285
1107	18-19	0.10965717676382525
1107	20-21	0.15075522875585534
1107	22-23	0.25021138131303644
1107	24-25	0.3092900864942649
1107	26-27	0.24318856232477515
1107	28-29	0.2524553210508671
1107	30-31	0.3244535208081203
1107	32-33	0.3194869334431587
1107	34-35	0.2707596942189454
1107	36-37	0.33040331635825737
1107	38-39	0.31028586921980406
1107	40-41	0.2559171728355878
1107	42-43	0.27304346847757444
1107	44-45	0.2585872191343199
1107	46-47	0.2690473982117254
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2216	92-93	-0.8696525262039962
2216	94-95	-0.891660929408232
2216	96-97	-0.8885309813655269
2216	98-99	-0.9293588683595466
2216	100	-0.5270166008157098
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	1.0
2	10.0
3	29.0
4	45.0
5	22.0
6	52.0
7	157.0
8	518.0
9	915.0
10	1267.0
11	2117.0
12	3554.0
13	5517.0
14	7650.0
15	10520.0
16	13015.0
17	15771.0
18	18170.0
19	20942.0
20	24412.0
21	28301.0
22	32276.0
23	38062.0
24	44256.0
25	51176.0
26	56838.0
27	61551.0
28	70646.0
29	85982.0
30	99248.0
31	115005.0
32	143474.0
33	186141.0
34	267932.0
35	382719.0
36	619051.0
37	1084611.0
38	999377.0
39	362352.0
40	842.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.07265059262566	20.572249710313578	12.219037148702398	24.13606254835836
2	15.589952914846245	19.785900163652634	40.65060162531952	23.9735452961816
3	18.46918996512056	26.385716631277695	29.062787318642258	26.082306084959484
4	11.870718427584839	16.559633150374108	40.29190247311183	31.27774594892922
5	12.916456976009055	38.649955411337366	35.477558069806335	12.956029542847244
6	30.056952443772154	38.664152384609345	18.102892612291402	13.176002559327094
7	26.93984491869994	31.933535290862924	23.707515069566583	17.41910472087055
8	26.09801406792866	35.310702807282226	19.99480476247959	18.596478362309522
9	26.039114692306693	15.116923641665567	21.227551466239625	37.61641019978811
10-11	25.426676279740445	26.018776393037392	27.993614172417345	20.560933154804818
12-13	25.55584108599988	23.913719451747216	27.861489696442227	22.668949765810677
14-15	22.8905297198888	25.267984868118226	25.13999071174618	26.70149470024679
16-17	22.060590991802346	27.815601213760306	26.378491463745856	23.74531633069149
18-19	21.464501642822565	28.535508657005426	27.88481702355571	22.1151726766163
20-21	23.180852484978562	27.70530346359552	26.932151414410427	22.18169263701549
22-23	23.659194742815448	27.284941903442846	26.617442531988356	22.43842082175335
24-25	22.982695131416968	27.2931433119812	27.47768449380283	22.246477062799002
26-27	22.93005216436539	27.58208973671647	26.44271370060054	23.045144398317596
28-29	22.073767682086245	28.08885971916311	27.004368042835004	22.833004555915647
30-31	22.85510989962905	27.692879272017574	27.04239319711439	22.409617631238987
32-33	23.1862403574267	27.895110144627782	26.143563975020673	22.77508552292484
34-35	23.185937819944733	27.712152842582142	27.034225357277197	22.067683980195923
36-37	22.80845563801906	27.348317949195472	26.707575439820985	23.135650972964488
38-39	22.898515285426082	27.522584055577447	27.27878482114145	22.30011583785502
40-41	22.608802238052665	26.609578881880097	27.889001734573217	22.892617145494025
42-43	22.56783381141128	26.84542105162315	27.249381980468545	23.33736315649703
44-45	22.805115152536587	27.77977231260355	27.089482409799608	22.325630125060254
46-47	22.697027248539133	27.53749883076913	26.60650261310223	23.158971307589514
48-49	23.287031666680747	27.220492757642432	26.889285810682317	22.603189764994504
50-51	22.951161820669373	26.997870966366172	26.95964169353927	23.09132551942518
52-53	22.44806058724179	27.601544846093347	26.74698179526957	23.203412771395293
54-55	22.410166569350466	27.504680037102403	26.788602425336773	23.29655096821036
56-57	23.475276189809062	26.994858123243315	26.484584928527244	23.045280758420382
58-59	22.426575118034442	27.369754173544624	27.323150244310074	22.880520464110855
60-61	22.46431363823192	27.95097393868034	26.687403231937402	22.897309191150338
62-63	22.885152930550838	27.277631414133758	26.298525247797265	23.538690407518136
64-65	22.04585606954833	27.54171166010517	26.786573858375302	23.6258584119712
66-67	22.750901604810206	27.203928019951196	26.69701030832258	23.34816006691602
68-69	22.314238056361894	27.255756156940837	26.947139169348343	23.482866617348925
70-71	22.26105657008873	26.95408524784288	27.06714446270967	23.717713719358724
72-73	22.78775947620427	27.03986068701425	27.47395709941233	22.698422737369146
74-75	23.041214759480138	27.28732077796193	26.952110285096825	22.719354177461106
76-77	22.736626743710776	27.239475518828122	26.67934139483677	23.344556342624333
78-79	22.67151632768999	27.205084109376955	26.68168537051544	23.441714192417614
80-81	22.217382024735755	27.606187111482306	27.237812488025405	22.938618375756537
82-83	23.043083219608757	26.669510939859585	28.177803194492355	22.1096026460393
84-85	23.192807664523315	27.24623740768053	26.921319847412462	22.639635080383698
86-87	21.97170372219908	27.84352334723096	27.385834885640204	22.798938044929756
88-89	23.118311302096224	26.673809037478495	27.089547757317195	23.118331903108086
90-91	22.710713344641125	26.767662824861176	27.23447484600467	23.28714898449303
92-93	23.068747610916564	26.93595458941448	27.172104961351828	22.823192838317134
94-95	21.98947227792214	27.03622982172009	27.771880745421974	23.202417154935794
96-97	22.357431180413144	26.929746610588413	27.30511353353246	23.407708675465987
98-99	21.75152838441442	27.618530330309852	27.147038764532027	23.482902520743703
100	22.553198344311635	27.006092969520285	26.346403347064644	24.094305339103432
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	990.0
1	708.5
2	504.0
3	570.0
4	541.0
5	486.0
6	429.5
7	442.0
8	509.5
9	521.0
10	469.5
11	400.5
12	396.0
13	436.5
14	479.0
15	793.5
16	1010.0
17	1120.5
18	1535.0
19	2009.0
20	2669.5
21	3514.0
22	4782.0
23	6567.0
24	8535.5
25	10893.5
26	14623.5
27	18745.5
28	23757.5
29	33764.5
30	47709.0
31	60923.5
32	76266.5
33	90695.0
34	101177.5
35	113423.0
36	124878.0
37	144563.5
38	152518.5
39	144040.5
40	137736.5
41	129773.0
42	128202.5
43	135373.0
44	147560.0
45	174028.5
46	199739.0
47	215981.0
48	226531.5
49	222470.0
50	211397.0
51	215357.0
52	216478.0
53	204016.0
54	193081.0
55	168461.0
56	148578.0
57	124923.5
58	99462.5
59	94519.5
60	78554.5
61	54806.5
62	41061.5
63	33570.5
64	24329.0
65	12040.5
66	6583.5
67	4344.5
68	2816.5
69	1763.5
70	1055.0
71	650.5
72	383.0
73	217.5
74	138.0
75	92.0
76	59.5
77	53.5
78	59.0
79	51.5
80	35.5
81	33.0
82	31.0
83	24.0
84	17.5
85	15.5
86	13.5
87	13.5
88	15.0
89	12.5
90	14.0
91	15.0
92	10.5
93	9.0
94	7.5
95	8.5
96	9.5
97	5.5
98	3.0
99	6.0
100	13.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05615380622281402
2	0.007003776271370788
3	0.0077247532404824855
4	0.009001912442908924
5	0.0031105006381676147
6	0.0025337190628782554
7	7.827749950355585E-4
8	0.001606748674020357
9	6.385796012132189E-4
10-11	4.943842073908792E-4
12-13	2.3689243270812958E-4
14-15	1.132963808604098E-4
16-17	4.8408453640356915E-4
18-19	0.0015243513061218772
20-21	9.372700598452084E-4
22-23	0.0012874588734137476
24-25	0.0010711657826802381
26-27	0.0032237970190280244
28-29	0.0036254841875331136
30-31	0.004758447996137212
32-33	0.00556182233314739
34-35	0.003501888135685394
36-37	0.008033743370101785
38-39	0.0066947861417514885
40-41	0.006262199960284468
42-43	0.014687330827904034
44-45	0.020259452832038734
46-47	0.018663003829005684
48-49	0.02189710051902102
50-51	0.018817498893815336
52-53	0.025234193918909454
54-55	0.019157388036396564
56-57	0.015480405493926902
58-59	0.01763303673027469
60-61	0.016046887398228953
62-63	0.0114532341378887
64-65	0.01185492130639379
66-67	0.013986953200766953
68-69	0.005912011146715929
70-71	0.008868016720073894
72-73	0.008610524945391143
74-75	0.011515032163812559
76-77	0.010464465723106942
78-79	0.011875520648368408
80-81	0.01025847230336074
82-83	0.006705085812738798
84-85	0.01078375552371355
86-87	0.009434498624375943
88-89	0.008105841067012955
90-91	0.013245376889680638
92-93	0.008517827906505354
94-95	0.007982245015165236
96-97	0.009753788424982552
98-99	0.009485996979312492
100	0.2585217417814805
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4854524.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.34621946442844
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.68752281903738	50.29922303025399
2	15.19451661905855	20.46586501766045
3	5.244329156442438	10.5955722694042
4	2.2492651140050857	6.059180080058766
5	1.057391733859012	3.560566788417077
6	0.5827927610910633	2.3549333514431376
7	0.32435215420462815	1.5290723952577518
8	0.2047212434174257	1.1029761430576501
9	0.1256886315235567	0.7618188746492216
>10	0.32667721723946563	3.062029087066985
>50	0.002216102846169846	0.10026077170490102
>100	4.8314117037189436E-4	0.06799197216298633
>500	0.0	0.0
>1k	4.330610479888391E-5	0.040510218862919556
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.059934197461996E-5	0.0
4	0.0	0.0	0.0	2.059934197461996E-5	0.0
5	0.0	0.0	0.0	2.059934197461996E-5	0.0
6	0.0	0.0	0.0	2.059934197461996E-5	2.059934197461996E-5
7	0.0	0.0	0.0	2.059934197461996E-5	2.059934197461996E-5
8	0.0	0.0	0.0	2.059934197461996E-5	2.059934197461996E-5
9	0.0	0.0	0.0	2.059934197461996E-5	2.059934197461996E-5
10-11	0.0	0.0	0.0	2.059934197461996E-5	2.059934197461996E-5
12-13	0.0	0.0	0.0	2.059934197461996E-5	2.059934197461996E-5
14-15	0.0	0.0	0.0	2.059934197461996E-5	4.119868394923993E-5
16-17	0.0	0.0	0.0	2.059934197461996E-5	6.17980259238599E-5
18-19	0.0	0.0	0.0	4.119868394923992E-5	6.17980259238599E-5
20-21	0.0	0.0	0.0	6.179802592385988E-5	8.239736789847985E-5
22-23	0.0	0.0	0.0	1.235960518477198E-4	1.1329638086040979E-4
24-25	0.0	0.0	0.0	2.265927617208196E-4	1.7509440678426967E-4
26-27	0.0	0.0	0.0	4.1198683949239924E-4	1.8539407777157967E-4
28-29	0.0	0.0	0.0	0.0024101230110305356	1.8539407777157967E-4
30-31	0.0	0.0	0.0	0.009074010139820093	1.8539407777157967E-4
32-33	0.0	0.0	0.0	0.020362449541911833	1.8539407777157967E-4
34-35	0.0	0.0	0.0	0.03853106916352664	1.8539407777157967E-4
36-37	0.0	0.0	0.0	0.07061454428899723	1.8539407777157967E-4
38-39	0.0	0.0	0.0	0.1194967827947704	2.0599341974619962E-4
40-41	0.0	0.0	0.0	0.1754445955978382	2.0599341974619962E-4
42-43	0.0	0.0	0.0	0.23429691561932747	2.0599341974619962E-4
44-45	0.0	0.0	0.0	0.30605472338791606	2.0599341974619962E-4
46-47	0.0	0.0	0.0	0.39298394652081237	2.265927617208196E-4
48-49	0.0	0.0	0.0	0.4945902008106253	2.6779144567005953E-4
50-51	0.0	0.0	0.0	0.609575727712954	2.780911166573695E-4
52-53	0.0	0.0	0.0	0.720585581614181	2.986904586319895E-4
54-55	0.0	0.0	0.0	0.8435121548477256	3.0899012961929943E-4
56-57	0.0	0.0	0.0	0.9955146992784463	3.0899012961929943E-4
58-59	0.0	0.0	0.0	1.1808572786950893	3.0899012961929943E-4
60-61	0.0	0.0	0.0	1.3732345333960652	3.0899012961929943E-4
62-63	0.0	0.0	0.0	1.5726773623943358	3.0899012961929943E-4
64-65	0.0	0.0	0.0	1.7885996649723022	3.0899012961929943E-4
66-67	0.0	0.0	0.0	2.031146617052465	3.0899012961929943E-4
68-69	0.0	0.0	0.0	2.308454958714799	3.0899012961929943E-4
70-71	0.0	0.0	0.0	2.615807440647116	3.0899012961929943E-4
72-73	0.0	0.0	0.0	2.94066936325786	3.604884845558494E-4
74-75	0.0	0.0	0.0	3.2577035359182487	3.7078815554315934E-4
76-77	0.0	0.0	0.0	3.625834376346682	3.7078815554315934E-4
78-79	0.0	0.0	0.0	4.022237813635281	3.7078815554315934E-4
80-81	0.0	0.0	0.0	4.454700399050452	4.2228651047970925E-4
82-83	0.0	0.0	0.0	4.907391538284701	4.943842073908792E-4
84-85	0.0	0.0	0.0	5.352759611446972	4.943842073908792E-4
86-87	0.0	0.0	0.0	5.823495774250987	5.046838783781891E-4
88	0.0	0.0	0.0	6.202647262635843	5.355828913401191E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	5125	0.0	39.98948	94
GTACTAG	5440	0.0	36.04316	1
GCATCGT	5345	0.0	33.674034	8
CTGTGCG	6740	0.0	31.933075	9
CAACGCA	8195	0.0	31.884443	5
TAGGCAT	6530	0.0	31.881855	5
TAGTACT	7055	0.0	30.843513	4
GTATCAA	9790	0.0	29.970058	1
GGTATCA	6365	0.0	29.697052	1
ACTGTGC	7790	0.0	29.378902	8
ATCAACG	8990	0.0	29.223482	3
CTAGGCA	7335	0.0	29.153528	4
TCTAACG	2260	0.0	29.11384	2
CTAACGC	2335	0.0	28.581257	3
CTAGTAC	8115	0.0	27.79922	3
GTACTGT	8180	0.0	27.51888	6
TAACGCC	2460	0.0	27.128956	4
TACTGTG	8670	0.0	26.993196	7
CATCGTC	6970	0.0	26.09236	9
GGCGTCG	3405	0.0	26.084877	8
>>END_MODULE