FastQCFastQC Report
Mon 16 Jan 2017
ERR1146642_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146642_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4854524
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146520.3018215586121317No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123120.25361909839152097No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88320.18193338831984351No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93400.047.6441
GTATCAA171250.041.4223481
GGGCACC49400.040.36453294
CAACGCA207700.032.7890555
GTACTAG51200.032.6153761
ATCAACG210300.032.3659823
CTGTGCG67550.032.2202349
TATCAAC227250.031.0078662
GTGGTAT23450.030.8917031
GAACCGT4350.030.2509446
TAGTACT73550.030.1869224
TAGGCAT62800.030.085935
AACGCAG234750.030.0700746
ACTGTGC80000.028.3750278
TCAACGC246600.027.9068474
CTAGTAC83900.027.8646723
GTACTGT82150.027.5746276
TACTGTG89850.027.5129937
TCTAACG20650.027.3353842
CTAACGC20400.027.2089213
Produced by FastQC (version 0.11.5)