FastQCFastQC Report
Mon 16 Jan 2017
ERR1146641_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146641_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3631961
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG27700.037.854451
CAACGCA58000.032.7340745
GTATCAA63100.032.71351
GCATCGT30200.031.8988938
ATCAACG60450.031.4868563
GGTATCA41050.030.1255131
TAGGCAT38650.029.4247325
GGGCACC34550.028.23322194
TAGTACT37850.027.9370944
CTAGGCA42750.027.5932064
AACGCAG71000.027.401276
CTGTGCG39650.027.2584659
CTAGTAC40450.027.1874123
ACGCAGA70050.027.0349527
CTAACGC13750.026.3183463
TATCAAC77700.026.2509632
GTACATG133350.025.987611
TCTAACG14150.025.9068582
CGCAGAG72600.025.8911938
GAGTTGC35200.025.5698994
Produced by FastQC (version 0.11.5)