##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146641_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3631961
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.39930907848405	31.0	31.0	34.0	28.0	34.0
2	31.445230551759778	33.0	31.0	34.0	28.0	34.0
3	31.58492946372497	33.0	31.0	34.0	28.0	34.0
4	34.99398561823764	37.0	35.0	37.0	32.0	37.0
5	35.15227311086215	37.0	35.0	37.0	32.0	37.0
6	35.212052387126406	37.0	35.0	37.0	32.0	37.0
7	35.16525673045498	37.0	35.0	37.0	32.0	37.0
8	35.10621176824311	37.0	35.0	37.0	32.0	37.0
9	36.61411369780678	39.0	37.0	39.0	32.0	39.0
10-11	36.46628309059486	39.0	37.0	39.0	32.0	39.0
12-13	36.40699184269875	39.0	37.0	39.0	32.0	39.0
14-15	37.61438558949284	40.0	38.0	41.0	31.5	41.0
16-17	37.637444344804365	40.0	38.0	41.0	31.5	41.0
18-19	37.603327926704054	40.0	37.5	41.0	31.0	41.0
20-21	37.674485629113306	40.0	37.5	41.0	31.0	41.0
22-23	37.62193178836446	40.0	37.0	41.0	31.0	41.0
24-25	37.59269207461203	40.0	37.0	41.0	31.0	41.0
26-27	37.6453985877051	40.0	37.0	41.0	31.0	41.0
28-29	37.63364914986697	40.0	37.0	41.0	31.0	41.0
30-31	37.501818439129714	40.0	37.0	41.0	31.0	41.0
32-33	37.50097963606988	40.0	37.0	41.0	31.0	41.0
34-35	37.44027317473949	40.0	37.0	41.0	30.5	41.0
36-37	37.32237020166241	40.0	36.5	41.0	30.0	41.0
38-39	37.22926003335388	40.0	36.0	41.0	30.0	41.0
40-41	37.1436186952448	40.0	36.0	41.0	30.0	41.0
42-43	36.9755751782577	40.0	36.0	41.0	30.0	41.0
44-45	36.839537373887	39.5	35.0	41.0	30.0	41.0
46-47	36.84208117873513	39.0	35.0	41.0	30.0	41.0
48-49	36.72094414009402	39.0	35.0	41.0	30.0	41.0
50-51	36.559811077266524	39.0	35.0	41.0	29.0	41.0
52-53	36.369247632339665	39.0	35.0	41.0	29.0	41.0
54-55	36.151056550442036	38.5	35.0	40.5	29.0	41.0
56-57	35.93442826065588	38.0	34.5	40.0	28.0	41.0
58-59	35.66706622125072	38.0	34.0	40.0	28.0	41.0
60-61	35.40655461333423	37.0	34.0	40.0	28.0	41.0
62-63	35.100436651164486	36.5	34.0	40.0	27.5	41.0
64-65	34.936219579450324	36.0	34.0	39.5	28.0	41.0
66-67	34.66319310697444	36.0	33.5	39.0	27.5	41.0
68-69	34.52291475596792	35.0	33.0	39.0	27.5	41.0
70-71	34.3800649291113	35.0	33.5	39.0	27.5	41.0
72-73	34.13277152480437	35.0	33.0	37.5	27.0	40.0
74-75	33.85860572291388	35.0	33.0	37.0	27.0	39.5
76-77	33.57692552315402	35.0	33.0	36.5	26.5	39.0
78-79	33.3156921288527	35.0	33.0	36.0	26.0	39.0
80-81	33.10731475365512	35.0	33.0	36.0	26.0	38.0
82-83	32.864521122335844	35.0	33.0	35.5	26.0	37.0
84-85	32.683580578095416	35.0	33.0	35.0	26.0	37.0
86-87	32.512116457197635	35.0	33.0	35.0	25.5	36.5
88-89	32.37183755552441	35.0	32.0	35.0	25.0	36.0
90-91	32.23088381180304	35.0	32.0	35.0	25.0	36.0
92-93	32.09323806065098	35.0	32.0	35.0	25.0	36.0
94-95	32.01339730795567	35.0	32.0	35.0	25.0	35.0
96-97	31.915121197611978	35.0	32.0	35.0	25.0	35.0
98-99	31.818954003085388	35.0	32.0	35.0	24.5	35.0
100	30.869580648030087	34.0	30.0	35.0	21.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3517478633082227
1101	2	0.4499140115841911
1101	3	0.46015233885178475
1101	4	0.5477207478134432
1101	5	0.46118156190483717
1101	6	0.3856857129882627
1101	7	0.4644254899023963
1101	8	0.5107966131531185
1101	9	0.6724113299546701
1101	10-11	0.6892145466998087
1101	12-13	0.5957692413695028
1101	14-15	0.7802530325255859
1101	16-17	0.7578302528785059
1101	18-19	0.7667780067001573
1101	20-21	0.7603961990755366
1101	22-23	0.7841614000063686
1101	24-25	0.7593063539123861
1101	26-27	0.7157565257082581
1101	28-29	0.6790994538830901
1101	30-31	0.7201500663669691
1101	32-33	0.6702347854865565
1101	34-35	0.6984818373163577
1101	36-37	0.7561485485583077
1101	38-39	0.6646715240213084
1101	40-41	0.7245181914306187
1101	42-43	0.6466837450209937
1101	44-45	0.6101576315559925
1101	46-47	0.5857462884942066
1101	48-49	0.6347978764314988
1101	50-51	0.5389958009971494
1101	52-53	0.520793524189294
1101	54-55	0.5553500971692245
1101	56-57	0.5026533362968024
1101	58-59	0.5113107818680831
1101	60-61	0.47275008465356194
1101	62-63	0.49821050198241323
1101	64-65	0.5412805535213678
1101	66-67	0.5076037438430916
1101	68-69	0.5370999156094598
1101	70-71	0.5776214289179009
1101	72-73	0.551805743134544
1101	74-75	0.49857376054598035
1101	76-77	0.5512852493071492
1101	78-79	0.49348785023617125
1101	80-81	0.4662799508200166
1101	82-83	0.5026128415681441
1101	84-85	0.48382963784086286
1101	86-87	0.4340426235907273
1101	88-89	0.5392480701543931
1101	90-91	0.5689071179017873
1101	92-93	0.505038611315392
1101	94-95	0.531736752474103
1101	96-97	0.533751946937457
1101	98-99	0.5712498782462418
1101	100	0.5486263335318355
1102	1	0.32124894252973135
1102	2	0.4385027682940681
1102	3	0.3856367920327699
1102	4	0.45911497691939473
1102	5	0.4111092992396337
1102	6	0.4057049975773168
1102	7	0.3882982880797812
1102	8	0.3983127005265814
1102	9	0.5270644842261518
1102	10-11	0.6510286985461917
1102	12-13	0.5670230620720034
1102	14-15	0.677282396832652
1102	16-17	0.6677761465261085
1102	18-19	0.6421618171550065
1102	20-21	0.5811614134807499
1102	22-23	0.6894708851768243
1102	24-25	0.755289077502681
1102	26-27	0.6791674066201523
1102	28-29	0.6479845351378586
1102	30-31	0.7108117796076954
1102	32-33	0.6087650972790186
1102	34-35	0.6607245990761825
1102	36-37	0.7116185706774374
1102	38-39	0.739670432280711
1102	40-41	0.6784347904832444
1102	42-43	0.7419156973887553
1102	44-45	0.706287858738115
1102	46-47	0.6538044970207864
1102	48-49	0.5627174565852897
1102	50-51	0.5878589068382922
1102	52-53	0.54916243395202
1102	54-55	0.5743589373071245
1102	56-57	0.5833176077255757
1102	58-59	0.45204905893761094
1102	60-61	0.45801814913375694
1102	62-63	0.46353392887944267
1102	64-65	0.47606977070508805
1102	66-67	0.6141641195916279
1102	68-69	0.49053136972794675
1102	70-71	0.5012172834134887
1102	72-73	0.44176055909928635
1102	74-75	0.4552682117367297
1102	76-77	0.49752304194167607
1102	78-79	0.5052286416163341
1102	80-81	0.5196116782009028
1102	82-83	0.45699143476696946
1102	84-85	0.4020396182234691
1102	86-87	0.43780292105948604
1102	88-89	0.4722818965896991
1102	90-91	0.47394862880567246
1102	92-93	0.4666816862844314
1102	94-95	0.447037369698009
1102	96-97	0.5323539475603241
1102	98-99	0.44438175943702873
1102	100	0.42207654238588077
1103	1	0.34134165782439396
1103	2	0.4424849676207181
1103	3	0.39857020658458353
1103	4	0.5385001915322718
1103	5	0.3632887431417515
1103	6	0.3583426492961834
1103	7	0.35844153760925934
1103	8	0.4077232267170956
1103	9	0.4746738508804569
1103	10-11	0.6008372420911741
1103	12-13	0.5190771314635256
1103	14-15	0.6777735486782532
1103	16-17	0.663363568813466
1103	18-19	0.648123750371461
1103	20-21	0.5774361642076116
1103	22-23	0.6466941083671216
1103	24-25	0.643118720870774
1103	26-27	0.6403905798010499
1103	28-29	0.6648011665004177
1103	30-31	0.6819114422648482
1103	32-33	0.605312877286039
1103	34-35	0.6313256719918314
1103	36-37	0.6058187802489314
1103	38-39	0.6313350046364548
1103	40-41	0.5645603533982566
1103	42-43	0.5098045724827145
1103	44-45	0.47490171975356077
1103	46-47	0.4807696041629299
1103	48-49	0.5369508635755267
1103	50-51	0.4494889880828481
1103	52-53	0.4643698522988018
1103	54-55	0.4882526393782456
1103	56-57	0.5043730341235957
1103	58-59	0.474385762356512
1103	60-61	0.4307788694483392
1103	62-63	0.4399113887587589
1103	64-65	0.4378524408020823
1103	66-67	0.4812380553524136
1103	68-69	0.4515972439461322
1103	70-71	0.45665793545587974
1103	72-73	0.3955684843461924
1103	74-75	0.37699692650107863
1103	76-77	0.37716850936814694
1103	78-79	0.428804913010147
1103	80-81	0.35955287168869177
1103	82-83	0.3847338805891738
1103	84-85	0.40514939414590856
1103	86-87	0.39919230196379374
1103	88-89	0.5079893628398651
1103	90-91	0.39300750665482553
1103	92-93	0.38116821373106546
1103	94-95	0.41993611730342195
1103	96-97	0.4130551783465677
1103	98-99	0.362147914262799
1103	100	0.5075679853958555
1104	1	0.2602188050351337
1104	2	0.3006253478629972
1104	3	0.24868100139070037
1104	4	0.3626119838614912
1104	5	0.32785157653003694
1104	6	0.2774643560962531
1104	7	0.2652263589849895
1104	8	0.3150558800651382
1104	9	0.3801644150938941
1104	10-11	0.4859843186299386
1104	12-13	0.3629421134362545
1104	14-15	0.448011531642166
1104	16-17	0.42042561496158726
1104	18-19	0.393210764184623
1104	20-21	0.26883680049843406
1104	22-23	0.3776949059100829
1104	24-25	0.4692417070681927
1104	26-27	0.39909149881147243
1104	28-29	0.37507862638815226
1104	30-31	0.3613438487153644
1104	32-33	0.41631315836776395
1104	34-35	0.4662312401573203
1104	36-37	0.3950812273611888
1104	38-39	0.43979168106702105
1104	40-41	0.4709130182326078
1104	42-43	0.46483734201472515
1104	44-45	0.4043807199902574
1104	46-47	0.36972845527837706
1104	48-49	0.3699710840239021
1104	50-51	0.32257392072890667
1104	52-53	0.3743093801026447
1104	54-55	0.35838116161751543
1104	56-57	0.36858104146553217
1104	58-59	0.3199928433968111
1104	60-61	0.31473683029555843
1104	62-63	0.3602256455195203
1104	64-65	0.341599155791549
1104	66-67	0.2458974973734982
1104	68-69	0.32342229514316045
1104	70-71	0.3478851873854083
1104	72-73	0.33623319638536486
1104	74-75	0.3118635355248571
1104	76-77	0.24164263954702392
1104	78-79	0.32288046191202113
1104	80-81	0.2997880028883415
1104	82-83	0.2911621323665301
1104	84-85	0.2642150127264671
1104	86-87	0.26486383631070964
1104	88-89	0.27910486761403064
1104	90-91	0.3524435706019773
1104	92-93	0.3879873844026207
1104	94-95	0.4188664085083076
1104	96-97	0.41147799024160747
1104	98-99	0.2800461957016793
1104	100	0.36299238709333537
1105	1	0.15164861179740186
1105	2	0.20177341969108298
1105	3	0.19949921283777527
1105	4	0.29505684066791815
1105	5	0.20186988049572108
1105	6	0.201894591282759
1105	7	0.26517050613163207
1105	8	0.23582900183224353
1105	9	0.344923564448365
1105	10-11	0.3368938659195706
1105	12-13	0.2479849164346959
1105	14-15	0.4589594465565838
1105	16-17	0.4104111326452582
1105	18-19	0.3644754567705135
1105	20-21	0.34905776095724406
1105	22-23	0.4088997965173462
1105	24-25	0.3839383997907575
1105	26-27	0.3602744885292921
1105	28-29	0.4004503435545601
1105	30-31	0.4732936324005408
1105	32-33	0.41606109772602196
1105	34-35	0.4598036937928853
1105	36-37	0.4090569583107637
1105	38-39	0.4793345813151362
1105	40-41	0.3888661294336657
1105	42-43	0.40853966996165525
1105	44-45	0.4723581107552235
1105	46-47	0.3885658485665715
1105	48-49	0.3215270130999812
1105	50-51	0.3870898144796939
1105	52-53	0.44182016955972614
1105	54-55	0.35274150188624276
1105	56-57	0.4096012825395974
1105	58-59	0.3434814651849436
1105	60-61	0.35008531267497034
1105	62-63	0.3543300811560073
1105	64-65	0.4149965403009972
1105	66-67	0.36615631292503537
1105	68-69	0.36703144738883964
1105	70-71	0.3715597663206083
1105	72-73	0.39481984978752394
1105	74-75	0.32249085506099817
1105	76-77	0.29849194066355267
1105	78-79	0.32299758863097594
1105	80-81	0.26086539785868723
1105	82-83	0.3377601679231148
1105	84-85	0.26756536256245056
1105	86-87	0.3032041917714139
1105	88-89	0.27671926082863507
1105	90-91	0.2885419268196472
1105	92-93	0.2656995003978295
1105	94-95	0.25828557825893483
1105	96-97	0.3080765514721051
1105	98-99	0.26062349736491086
1105	100	0.2991742597540856
1106	1	0.14200164119672465
1106	2	0.21606162053710065
1106	3	0.17206806847364575
1106	4	0.27730810038881515
1106	5	0.12462815640640912
1106	6	0.1596043514572898
1106	7	0.19068248399077703
1106	8	0.2542921701484957
1106	9	0.3506917299983172
1106	10-11	0.36411186953227315
1106	12-13	0.27421748965490167
1106	14-15	0.40128960221852594
1106	16-17	0.34540061874849215
1106	18-19	0.33696698365269384
1106	20-21	0.3348748790273035
1106	22-23	0.350937797702386
1106	24-25	0.374711167113162
1106	26-27	0.3847135810525586
1106	28-29	0.37098702506315817
1106	30-31	0.4151210611892253
1106	32-33	0.34826915346161513
1106	34-35	0.3096779589079759
1106	36-37	0.33899344305391566
1106	38-39	0.39640997087897745
1106	40-41	0.42435107903664715
1106	42-43	0.2735108405851676
1106	44-45	0.29938248984311855
1106	46-47	0.2597851563147202
1106	48-49	0.20287110364218108
1106	50-51	0.2334573915655156
1106	52-53	0.16806797296180065
1106	54-55	0.27585754801978624
1106	56-57	0.1734088410940018
1106	58-59	0.15911837961225928
1106	60-61	0.19535417364385665
1106	62-63	0.2871831211886402
1106	64-65	0.21517720853790223
1106	66-67	0.1918531469121305
1106	68-69	0.23417613346224186
1106	70-71	0.24305832133460825
1106	72-73	0.23381385946744615
1106	74-75	0.19947253729102954
1106	76-77	0.2783482011189662
1106	78-79	0.19787452843429065
1106	80-81	0.1529628638247189
1106	82-83	0.19656918405991775
1106	84-85	0.24574366922143298
1106	86-87	0.2784127765554274
1106	88-89	0.32809913820848635
1106	90-91	0.21347090444093197
1106	92-93	0.1813145756277308
1106	94-95	0.20316463818208774
1106	96-97	0.22226260703821765
1106	98-99	0.15700077530771495
1106	100	0.1300935574578439
1107	1	0.037167687223444545
1107	2	0.07704635139468508
1107	3	0.12715229811889373
1107	4	0.18953233769637023
1107	5	0.06674634230600418
1107	6	0.14822102381798175
1107	7	0.07991179744704624
1107	8	0.0499717734815448
1107	9	0.05358192442287191
1107	10-11	0.2086147994055807
1107	12-13	0.0975956292955189
1107	14-15	0.22916756507656544
1107	16-17	0.1313344824900753
1107	18-19	0.06472759799550687
1107	20-21	0.06046503024122529
1107	22-23	0.18637247096305742
1107	24-25	0.278275876539098
1107	26-27	0.13779104319076652
1107	28-29	0.17904771164836575
1107	30-31	0.3059147677031717
1107	32-33	0.2175205116029204
1107	34-35	0.2279643063930834
1107	36-37	0.28832609850465474
1107	38-39	0.20154765327998803
1107	40-41	0.16998079701917135
1107	42-43	0.222916123999795
1107	44-45	0.23080234823041224
1107	46-47	0.18931369050193325
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2216	100	-0.7506072527090808
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	1.0
2	6.0
3	18.0
4	40.0
5	27.0
6	39.0
7	127.0
8	321.0
9	571.0
10	910.0
11	1704.0
12	3012.0
13	4364.0
14	5943.0
15	8103.0
16	9991.0
17	11921.0
18	13734.0
19	16395.0
20	18999.0
21	21594.0
22	24486.0
23	27849.0
24	32367.0
25	36993.0
26	41253.0
27	43974.0
28	51398.0
29	62557.0
30	73003.0
31	85036.0
32	105836.0
33	137913.0
34	195074.0
35	280907.0
36	455916.0
37	820726.0
38	782910.0
39	255303.0
40	640.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.39944646981247	19.22341026058511	11.828939005612717	24.54820426398971
2	16.13363956450997	19.677785398984877	39.207870519393374	24.980704517111782
3	18.932113689056795	25.32982162427099	28.7925081092393	26.94555657743291
4	12.446610865595822	15.94772098509992	38.43860721099925	33.167060938305006
5	13.524664981005333	38.15671473912863	34.705652197018075	13.61296808284797
6	31.752667018184873	37.161733709815756	17.41925980178228	13.666339470217093
7	28.114599188089635	31.662769746536824	22.476684559826076	17.745946505547465
8	26.76012516812756	34.90833348247413	19.538838326337483	18.792703023060835
9	26.280768327459064	15.308894059677733	20.193114370525294	38.21722324233791
10-11	25.666030598193007	25.76637623929758	27.632648223204832	20.934944939304582
12-13	25.747221949182713	23.530191607655716	27.558809268732276	23.163777174429296
14-15	23.097588985772685	25.028090916274614	24.627618113320175	27.246701984632526
16-17	22.768121722057305	27.777666879178973	25.700998073622504	23.753213325141214
18-19	22.325226017751014	28.05622669095144	27.064116489831093	22.55443080146645
20-21	23.6006712702923	27.2201204872354	26.44844875437785	22.730759488094453
22-23	23.810073173740275	26.922641677753596	26.21681355013789	23.05047159836824
24-25	23.416640819360303	27.00905158213737	26.765790914496286	22.808516684006044
26-27	23.288645497227495	27.2162592703087	26.08626217656227	23.408833055901532
28-29	22.74308758361561	27.56114189170213	26.445220796971636	23.250549727710627
30-31	23.25853541950075	27.316935698043114	26.451116654484796	22.973412227971345
32-33	23.428053295914914	27.464250439805017	25.801871453301917	23.30582481097815
34-35	23.54018256391279	27.271227900911498	26.513261655851167	22.67532787932455
36-37	23.3054717627962	26.911688780153348	26.274525731452275	23.508313725598178
38-39	23.319361051007874	27.204028180633166	26.5625129071287	22.91409786123026
40-41	23.142822133864946	26.556810547068604	27.05450860749177	23.24585871157468
42-43	23.03614618412273	26.734313801893656	26.591792736286045	23.637747277697567
44-45	23.17022004630742	27.30723658437679	26.522502748771572	23.00004062054422
46-47	23.21636637773754	27.15869988777892	26.211347410309195	23.41358632417435
48-49	23.544339146021112	27.065949695526864	26.261388223458482	23.12832293499354
50-51	23.200555624096726	26.943362473122068	26.428261813070602	23.4278200897106
52-53	23.04396219823522	27.238226331656517	26.24915846058153	23.46865300952673
54-55	22.864311263609956	27.185899251530792	26.39493733702925	23.55485214783
56-57	23.59332253753047	26.897686383533177	26.074369872687825	23.434621206248533
58-59	22.927782773729383	27.0388336213177	26.718491460365072	23.314892144587844
60-61	22.893506869025533	27.4848592288654	26.31632284247884	23.30531105963023
62-63	23.146235235302353	27.152752086357502	26.03707247277543	23.663940205564714
64-65	22.62045314264291	27.282045972393544	26.321135958626524	23.776364926337024
66-67	23.040338643361252	27.00454724322175	26.254705101102015	23.700409012314978
68-69	22.701416744166195	27.022951104049913	26.52521482456424	23.750417327219647
70-71	22.670345285632447	26.818029932400627	26.574749839362166	23.93687494260476
72-73	23.075011454905123	26.92303350119175	26.813468435127792	23.18848660877534
74-75	23.200724875933425	27.127663968621384	26.473350813626702	23.198260341818493
76-77	23.052809595030656	26.992742623562716	26.322642433464644	23.631805347941988
78-79	22.998749821009614	26.989643341006975	26.257779197462195	23.75382764052122
80-81	22.699624446744945	27.30591722518229	26.661995599430604	23.332462728642156
82-83	23.232523349148625	26.625945799190852	27.327060451020824	22.814470400639696
84-85	23.31704136271533	26.985894565217244	26.52916039694967	23.167903675117756
86-87	22.57063447076999	27.427623873379282	26.777084435062605	23.224657220788128
88-89	23.244672268148598	26.581577890869678	26.6138634199586	23.559886421023123
90-91	22.990934725618487	26.73144813076906	26.64959354966625	23.628023593946203
92-93	23.24676233002095	26.842440186539136	26.65894101371728	23.251856469722636
94-95	22.54895090405266	26.831975167378026	27.06664652903227	23.552427399537045
96-97	22.792653573958948	26.663424743273538	26.78755773299236	23.756363949775153
98-99	22.392872558151062	27.164395283342934	26.64707473777036	23.79565742073564
100	22.944469750300218	26.83556254917388	26.106836722017473	24.113130978508426
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1448.0
1	1079.5
2	865.5
3	1055.5
4	1020.5
5	927.0
6	870.0
7	795.0
8	746.0
9	725.0
10	676.5
11	595.5
12	567.5
13	592.0
14	632.0
15	798.5
16	938.5
17	1060.0
18	1335.0
19	1632.0
20	2148.5
21	2881.0
22	3836.5
23	5149.5
24	6619.0
25	8416.5
26	11004.5
27	14002.0
28	17738.5
29	24089.0
30	32629.0
31	40551.5
32	50469.0
33	59296.5
34	65074.0
35	72430.5
36	79605.5
37	90824.5
38	96563.5
39	94099.5
40	92418.0
41	90744.5
42	92303.5
43	99287.0
44	109454.0
45	125932.5
46	140815.5
47	151419.0
48	160584.0
49	161861.0
50	157422.5
51	160511.5
52	163270.5
53	158521.0
54	152819.5
55	137709.0
56	124187.5
57	108562.0
58	92187.5
59	85574.0
60	72307.5
61	55062.5
62	43217.0
63	34236.5
64	24552.0
65	14876.0
66	9552.5
67	6676.5
68	4470.0
69	2679.0
70	1566.5
71	837.0
72	438.5
73	205.0
74	106.0
75	87.0
76	75.5
77	53.5
78	41.0
79	33.0
80	31.5
81	29.5
82	25.0
83	20.0
84	18.0
85	19.5
86	16.0
87	11.5
88	9.0
89	6.0
90	5.0
91	5.0
92	6.0
93	7.0
94	3.5
95	1.0
96	2.5
97	3.0
98	4.0
99	4.0
100	7.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.059692270924715324
2	0.007764400553860572
3	0.008287533924510752
4	0.01018733406002983
5	0.004185066965201443
6	0.0026432001885482804
7	9.086000648134713E-4
8	0.0012665334236793844
9	5.506667059475583E-4
10-11	3.992333618119798E-4
12-13	2.2026668237902337E-4
14-15	1.1013334118951169E-4
16-17	3.992333618119798E-4
18-19	0.0015418667766531635
20-21	0.0011288667471924946
22-23	0.0015694001119505414
24-25	0.0015005667737070965
26-27	0.0037858336033894636
28-29	0.0044879336534726
30-31	0.005437833721232139
32-33	0.006552933800775944
34-35	0.003772066935740775
36-37	0.00870053395397142
38-39	0.0068007338184523465
40-41	0.006635533806668078
42-43	0.015638934448910655
44-45	0.02184770155846938
46-47	0.02026453477887015
48-49	0.023472168341014673
50-51	0.0200718014317885
52-53	0.026610968564915755
54-55	0.02063623480538475
56-57	0.016836634534346598
58-59	0.018929168016947318
60-61	0.017167034557915132
62-63	0.012458834222063507
64-65	0.012541434227955641
66-67	0.014592667707610297
68-69	0.006016033762477075
70-71	0.009485234009946693
72-73	0.009058467312837335
74-75	0.012197267536738417
76-77	0.011412567480763147
78-79	0.013243534278038779
80-81	0.010614100757139188
82-83	0.006718133812560212
84-85	0.011495167486655281
86-87	0.009898234039407362
88-89	0.008342600595105509
90-91	0.014344867689933895
92-93	0.009278733995216358
94-95	0.007888300562698774
96-97	0.010242400730624586
98-99	0.010008367380596872
100	0.26462288554309915
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3631961.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.37161191533589
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.41468735114736	52.92966960119709
2	14.00100881115465	19.14543081718928
3	4.4022284710682955	9.029623697595719
4	1.7814455638385192	4.872012189562558
5	0.8574262525339715	2.931180749213674
6	0.461387717569569	1.8927493180901498
7	0.2767796519580117	1.324670966481444
8	0.19370867726568236	1.0595339605313825
9	0.12646685384375564	0.778206838604289
>10	0.47170935845769885	5.154260205104901
>50	0.01036401991935716	0.48212658347721593
>100	0.002678697841622475	0.28020275635216196
>500	0.0	0.0
>1k	1.0857340147658323E-4	0.12033231660007632
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.5066670594755836E-5
2	0.0	0.0	0.0	0.0	8.260000589213375E-5
3	0.0	0.0	0.0	0.0	8.260000589213375E-5
4	0.0	0.0	0.0	0.0	8.260000589213375E-5
5	0.0	0.0	0.0	2.7533335297377918E-5	8.260000589213375E-5
6	0.0	0.0	0.0	2.7533335297377918E-5	1.1013334118951167E-4
7	0.0	0.0	0.0	5.5066670594755836E-5	1.1013334118951167E-4
8	0.0	0.0	0.0	5.5066670594755836E-5	1.1013334118951167E-4
9	0.0	0.0	0.0	5.5066670594755836E-5	1.1013334118951167E-4
10-11	0.0	0.0	0.0	5.5066670594755836E-5	1.1013334118951167E-4
12-13	0.0	0.0	0.0	5.5066670594755836E-5	2.2026668237902334E-4
14-15	0.0	0.0	0.0	5.5066670594755836E-5	2.340333500277123E-4
16-17	0.0	0.0	0.0	5.5066670594755836E-5	2.4780001767640126E-4
18-19	0.0	0.0	0.0	5.5066670594755836E-5	3.028666882711571E-4
20-21	0.0	0.0	0.0	9.636667354082272E-5	3.028666882711571E-4
22-23	0.0	0.0	0.0	1.652000117842675E-4	3.44166691217224E-4
24-25	0.0	0.0	0.0	3.028666882711571E-4	3.8546669416329087E-4
26-27	0.0	0.0	0.0	7.296333853805148E-4	4.130000294606688E-4
28-29	0.0	0.0	0.0	0.0033866002415774837	4.2676669710935773E-4
30-31	0.0	0.0	0.0	0.011440100816060525	4.5430003240673564E-4
32-33	0.0	0.0	0.0	0.025812501841291798	4.956000353528025E-4
34-35	0.0	0.0	0.0	0.04822463677335742	5.506667059475583E-4
36-37	0.0	0.0	0.0	0.0850367060659517	5.506667059475583E-4
38-39	0.0	0.0	0.0	0.14159017676676594	5.782000412449362E-4
40-41	0.0	0.0	0.0	0.20429734790654414	6.057333765423142E-4
42-43	0.0	0.0	0.0	0.2703222859496564	6.608000471370701E-4
44-45	0.0	0.0	0.0	0.35040299166208005	7.571667206778928E-4
46-47	0.0	0.0	0.0	0.45519486580390045	8.535333942187155E-4
48-49	0.0	0.0	0.0	0.5715782741059169	9.636667354082271E-4
50-51	0.0	0.0	0.0	0.6932480827850299	9.774334030569162E-4
52-53	0.0	0.0	0.0	0.8162945582290118	0.001018733406002983
54-55	0.0	0.0	0.0	0.9544981347542003	0.0010462667413003608
56-57	0.0	0.0	0.0	1.1227543467564767	0.0010462667413003608
58-59	0.0	0.0	0.0	1.3236375610861462	0.0010462667413003608
60-61	0.0	0.0	0.0	1.534542909464061	0.0010462667413003608
62-63	0.0	0.0	0.0	1.754782058507787	0.0010462667413003608
64-65	0.0	0.0	0.0	1.9801561745844738	0.0010462667413003608
66-67	0.0	0.0	2.7533335297377918E-5	2.2437052600509753	0.0010738000765977388
68-69	0.0	0.0	2.7533335297377918E-5	2.5465306483191865	0.0011013334118951167
70-71	0.0	0.0	2.7533335297377918E-5	2.8810744388499767	0.0011288667471924946
72-73	0.0	0.0	2.7533335297377918E-5	3.229742830388322	0.001294066758976762
74-75	0.0	0.0	2.7533335297377918E-5	3.5723263548259467	0.001294066758976762
76-77	0.0	0.0	2.7533335297377918E-5	3.956980815597965	0.001294066758976762
78-79	0.0	0.0	2.7533335297377918E-5	4.381407179207045	0.001294066758976762
80-81	0.0	0.0	2.7533335297377918E-5	4.851414979400936	0.001294066758976762
82-83	0.0	0.0	2.7533335297377918E-5	5.342485781097318	0.001376666764868896
84-85	0.0	2.7533335297377918E-5	2.7533335297377918E-5	5.817449581644737	0.001376666764868896
86-87	0.0	2.7533335297377918E-5	2.7533335297377918E-5	6.314894350462463	0.001376666764868896
88	0.0	2.7533335297377918E-5	2.7533335297377918E-5	6.7153804790304745	0.0014317334354636518
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	2770	0.0	37.85445	1
CAACGCA	5800	0.0	32.734074	5
GTATCAA	6310	0.0	32.7135	1
GCATCGT	3020	0.0	31.898893	8
ATCAACG	6045	0.0	31.486856	3
GGTATCA	4105	0.0	30.125513	1
TAGGCAT	3865	0.0	29.424732	5
GGGCACC	3455	0.0	28.233221	94
TAGTACT	3785	0.0	27.937094	4
CTAGGCA	4275	0.0	27.593206	4
AACGCAG	7100	0.0	27.40127	6
CTGTGCG	3965	0.0	27.258465	9
CTAGTAC	4045	0.0	27.187412	3
ACGCAGA	7005	0.0	27.034952	7
CTAACGC	1375	0.0	26.318346	3
TATCAAC	7770	0.0	26.250963	2
GTACATG	13335	0.0	25.98761	1
TCTAACG	1415	0.0	25.906858	2
CGCAGAG	7260	0.0	25.891193	8
GAGTTGC	3520	0.0	25.56989	94
>>END_MODULE