FastQCFastQC Report
Mon 16 Jan 2017
ERR1146641_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146641_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3631961
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128420.3535830918889272No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104660.2881638872223573No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76630.21098794838380697No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66400.048.6055871
GTATCAA120550.041.7977031
GTACTAG26250.032.2606661
CAACGCA152950.032.1114775
ATCAACG155050.031.7062443
TATCAAC163750.030.8282282
AACGCAG168050.029.97966
GGGCACC34400.029.52759294
CTGTGCG39550.029.2368059
TAGTACT38050.029.1781394
GTGGTAT21750.028.9851591
TCAACGC170850.028.8842284
CTAGTAC41050.027.1603553
ACGCAGA186750.026.2478227
ACTGTGC50150.026.1462148
CGCAGAG188950.025.9674388
GTACTGT45450.025.7474966
TACTGTG51800.024.8593837
CCTAGTA45700.024.5014022
GCAGAGT203950.023.8999149
Produced by FastQC (version 0.11.5)