FastQCFastQC Report
Mon 16 Jan 2017
ERR1146640_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146640_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5198191
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC96340.18533370551409134No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC64450.047.65985594
GTACTAG79000.036.5471341
TATTCTC27600.034.7325335
GCATCGT78700.034.510218
TAGGCAT91100.034.2503555
TACCCCG3350.033.6652455
CTGTGCG96750.033.1714329
TAGTACT98350.033.0174944
ACTGTGC99250.032.9040078
TACCTGG13300.032.8612632
CAACGCA101250.032.5340655
TACTGTG112150.031.0046587
CTAGGCA104300.030.728444
GTATCAA123200.030.5345381
GTACTGT105200.030.462336
ATCAACG110050.029.7207413
AGGGCTA69250.029.0428565
CAGTACG8750.028.998819
CATGGGT9950.028.810264
TCTCTGC32700.028.451948
Produced by FastQC (version 0.11.5)