##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146640_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5198191
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.483598236386467	31.0	31.0	34.0	28.0	34.0
2	31.581771812540172	33.0	31.0	34.0	28.0	34.0
3	31.66526605120897	33.0	31.0	34.0	28.0	34.0
4	35.064074790633896	37.0	35.0	37.0	32.0	37.0
5	35.22020179712519	37.0	35.0	37.0	32.0	37.0
6	35.33757089726022	37.0	35.0	37.0	32.0	37.0
7	35.28190499348716	37.0	35.0	37.0	32.0	37.0
8	35.27568417551414	37.0	35.0	37.0	32.0	37.0
9	36.775321068425534	39.0	37.0	39.0	32.0	39.0
10-11	36.60039348304054	39.0	37.0	39.0	32.0	39.0
12-13	36.518035120294726	39.0	37.0	39.0	32.0	39.0
14-15	37.70745572834858	40.0	38.0	41.0	32.0	41.0
16-17	37.75363554359584	40.0	38.0	41.0	32.0	41.0
18-19	37.73601172023113	40.0	38.0	41.0	32.0	41.0
20-21	37.81079379345623	40.0	38.0	41.0	32.0	41.0
22-23	37.759002506833625	40.0	37.5	41.0	31.5	41.0
24-25	37.725899740890625	40.0	37.0	41.0	31.5	41.0
26-27	37.7642840557417	40.0	37.0	41.0	32.0	41.0
28-29	37.77428234168387	40.0	37.0	41.0	32.0	41.0
30-31	37.6551030925951	40.0	37.0	41.0	31.0	41.0
32-33	37.65818695388454	40.0	37.0	41.0	31.0	41.0
34-35	37.60092789972512	40.0	37.0	41.0	31.0	41.0
36-37	37.48703991446255	40.0	36.5	41.0	31.0	41.0
38-39	37.37135938252365	40.0	36.0	41.0	31.0	41.0
40-41	37.27259040693195	40.0	36.0	41.0	30.0	41.0
42-43	37.10774479044729	40.0	35.5	41.0	30.0	41.0
44-45	36.92373423754533	39.0	35.0	41.0	30.0	41.0
46-47	36.89689595861329	39.0	35.0	41.0	30.0	41.0
48-49	36.73668560851265	39.0	35.0	41.0	30.0	41.0
50-51	36.52823559195882	39.0	35.0	41.0	30.0	41.0
52-53	36.28505791726391	38.5	35.0	40.5	29.0	41.0
54-55	36.05323986748467	38.0	35.0	40.0	29.0	41.0
56-57	35.78636106291593	37.5	34.0	40.0	29.0	41.0
58-59	35.446742914987155	37.0	34.0	40.0	28.0	41.0
60-61	35.19240385741886	36.5	34.0	40.0	28.0	41.0
62-63	34.86816759907437	36.0	34.0	39.0	28.0	41.0
64-65	34.70225055216324	35.0	34.0	39.0	28.0	41.0
66-67	34.455401985036715	35.0	33.0	39.0	28.0	41.0
68-69	34.32566271612566	35.0	33.0	38.5	28.0	41.0
70-71	34.276273803713636	35.0	34.0	37.5	28.0	41.0
72-73	34.075157684663765	35.0	33.0	37.0	28.0	40.0
74-75	33.83826104119683	35.0	33.0	36.5	27.0	39.5
76-77	33.596908520675754	35.0	33.0	36.0	27.0	39.0
78-79	33.363846288064444	35.0	33.0	36.0	27.0	39.0
80-81	33.214769426517805	35.0	33.0	36.0	27.0	38.5
82-83	32.95391646824828	35.0	33.0	35.0	26.0	37.0
84-85	32.79613907992223	35.0	33.0	35.0	26.0	37.0
86-87	32.6382148135765	35.0	33.0	35.0	26.0	36.5
88-89	32.5252967811302	35.0	33.0	35.0	26.0	36.0
90-91	32.37390911569044	35.0	33.0	35.0	25.0	36.0
92-93	32.21107833475145	35.0	32.5	35.0	25.0	36.0
94-95	32.1564014288817	35.0	32.0	35.0	25.0	36.0
96-97	32.04321522622004	35.0	32.0	35.0	25.0	35.0
98-99	31.954093356708132	35.0	32.0	35.0	25.0	35.0
100	31.013887715938104	34.0	30.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4334113228331873
1101	2	0.5089446905121378
1101	3	0.5563651410592243
1101	4	0.6318873323687342
1101	5	0.527458279812258
1101	6	0.49085036777253066
1101	7	0.5120853376956731
1101	8	0.506445223482423
1101	9	0.716917301823564
1101	10-11	0.7505158945581556
1101	12-13	0.6336339276750138
1101	14-15	0.8114071878460152
1101	16-17	0.8092761076224662
1101	18-19	0.8405725575662899
1101	20-21	0.8111760208805947
1101	22-23	0.8203364343589712
1101	24-25	0.8810116962600745
1101	26-27	0.7932534313222632
1101	28-29	0.8046350343502056
1101	30-31	0.8356418431310502
1101	32-33	0.8175708885535329
1101	34-35	0.8387658107789875
1101	36-37	0.8354036339093796
1101	38-39	0.8257093724646509
1101	40-41	0.8519349550079482
1101	42-43	0.8087771168582378
1101	44-45	0.7956406365477449
1101	46-47	0.8190796817233164
1101	48-49	0.7590086149610116
1101	50-51	0.7562848779946876
1101	52-53	0.6905789503901048
1101	54-55	0.6814138594690462
1101	56-57	0.7018491075862627
1101	58-59	0.6047492274650423
1101	60-61	0.5918401906810047
1101	62-63	0.6060564220229452
1101	64-65	0.6660619318811811
1101	66-67	0.6402293414014864
1101	68-69	0.6090578542001452
1101	70-71	0.6079392743514447
1101	72-73	0.6232468424672248
1101	74-75	0.6009510876437361
1101	76-77	0.6302618235226518
1101	78-79	0.6099426538017099
1101	80-81	0.6048473063042792
1101	82-83	0.6312883906448619
1101	84-85	0.6019696762924482
1101	86-87	0.5788972606997191
1101	88-89	0.6805166996908127
1101	90-91	0.6381916794116975
1101	92-93	0.6067499463547676
1101	94-95	0.6675954377429107
1101	96-97	0.5831993719853159
1101	98-99	0.5709760535408499
1101	100	0.6878547095799092
1102	1	0.3380745590660119
1102	2	0.427739965513112
1102	3	0.43671442375589464
1102	4	0.5112193327206
1102	5	0.4018658212149475
1102	6	0.4635664254474321
1102	7	0.446358530480353
1102	8	0.4665106548035567
1102	9	0.5112414780035976
1102	10-11	0.6382445380746589
1102	12-13	0.554768581143172
1102	14-15	0.7230556216279496
1102	16-17	0.6491261142973102
1102	18-19	0.6950259152216915
1102	20-21	0.6860119725565141
1102	22-23	0.6983539025667156
1102	24-25	0.6853958089674208
1102	26-27	0.7034309622557515
1102	28-29	0.6845971995491666
1102	30-31	0.707398582899323
1102	32-33	0.6289210524900284
1102	34-35	0.6889911682378695
1102	36-37	0.7359674939413097
1102	38-39	0.7327591892981857
1102	40-41	0.6578722197359497
1102	42-43	0.6917005249962855
1102	44-45	0.6590792548823856
1102	46-47	0.6693582779522345
1102	48-49	0.6057439055779597
1102	50-51	0.645103452594256
1102	52-53	0.5632745871419758
1102	54-55	0.5608791476637123
1102	56-57	0.5547036475205616
1102	58-59	0.504365171923908
1102	60-61	0.47575593458171994
1102	62-63	0.5117442591131791
1102	64-65	0.5031530956745129
1102	66-67	0.5675163547744901
1102	68-69	0.5151755107182225
1102	70-71	0.563177414751749
1102	72-73	0.5139235556001154
1102	74-75	0.5078876539038006
1102	76-77	0.4209246978482071
1102	78-79	0.42840164814825243
1102	80-81	0.49381276936139074
1102	82-83	0.4635009430158661
1102	84-85	0.49797771221930276
1102	86-87	0.4603140098276839
1102	88-89	0.5144153555464186
1102	90-91	0.37998432662116954
1102	92-93	0.46424395962961285
1102	94-95	0.5153659660617009
1102	96-97	0.45680482258804744
1102	98-99	0.4596098652436815
1102	100	0.5415595697668607
1103	1	0.23451792529801452
1103	2	0.29813083265578655
1103	3	0.3056113823595439
1103	4	0.43371089912850636
1103	5	0.23806015066998043
1103	6	0.2925276163201431
1103	7	0.29805819169887826
1103	8	0.3225406042857273
1103	9	0.41296887714815256
1103	10-11	0.5007431149627237
1103	12-13	0.3890330326315379
1103	14-15	0.5363726691840611
1103	16-17	0.5073560598165301
1103	18-19	0.529905653209056
1103	20-21	0.45584891567359875
1103	22-23	0.5578807742707212
1103	24-25	0.5306344200665194
1103	26-27	0.446524572703062
1103	28-29	0.4551767167403753
1103	30-31	0.4511833430236507
1103	32-33	0.46894623762011634
1103	34-35	0.4623480634701167
1103	36-37	0.4695150600726379
1103	38-39	0.4817633992891288
1103	40-41	0.45589159291571946
1103	42-43	0.42192658444227504
1103	44-45	0.3546246792516996
1103	46-47	0.3966271545939506
1103	48-49	0.39226568128136563
1103	50-51	0.358710120476303
1103	52-53	0.37271581202740833
1103	54-55	0.37203596413191065
1103	56-57	0.365389687812538
1103	58-59	0.3669422039819068
1103	60-61	0.375182611257749
1103	62-63	0.3219189996480196
1103	64-65	0.34741603405372246
1103	66-67	0.3277945193992906
1103	68-69	0.33863149138915105
1103	70-71	0.3017309300915656
1103	72-73	0.31846839938130245
1103	74-75	0.3095017315230635
1103	76-77	0.29907643132091977
1103	78-79	0.3495895239046689
1103	80-81	0.2930674027998208
1103	82-83	0.3006492609657343
1103	84-85	0.36782456812498054
1103	86-87	0.33349116893482744
1103	88-89	0.40718086587702373
1103	90-91	0.3886081546637641
1103	92-93	0.3614712995557383
1103	94-95	0.3118520089438661
1103	96-97	0.3841963916638136
1103	98-99	0.36733914359141195
1103	100	0.4585683417358233
1104	1	0.22555247416380908
1104	2	0.32607372849773597
1104	3	0.2793968380532128
1104	4	0.4271738748366758
1104	5	0.2975051860557869
1104	6	0.3026665279808327
1104	7	0.33454281999060953
1104	8	0.35705715813181627
1104	9	0.42127493834272656
1104	10-11	0.5391659451867739
1104	12-13	0.4063996069114637
1104	14-15	0.49572548046526776
1104	16-17	0.46197003874144116
1104	18-19	0.4996816672639852
1104	20-21	0.46175147614387413
1104	22-23	0.5029454163975018
1104	24-25	0.5195558536851621
1104	26-27	0.44157009101910916
1104	28-29	0.4229152717608926
1104	30-31	0.44201827192116383
1104	32-33	0.47095103862849186
1104	34-35	0.4826909580560539
1104	36-37	0.46905328959672943
1104	38-39	0.43793771268894943
1104	40-41	0.49038277327350954
1104	42-43	0.5293104704303246
1104	44-45	0.4981882558607964
1104	46-47	0.40126017967288163
1104	48-49	0.408641955155403
1104	50-51	0.4008376488101959
1104	52-53	0.44481110978486527
1104	54-55	0.4556333950181113
1104	56-57	0.4522582728612292
1104	58-59	0.40289166775889385
1104	60-61	0.3516611259400051
1104	62-63	0.43328628695167026
1104	64-65	0.48181599984425105
1104	66-67	0.45685180432866446
1104	68-69	0.42127707882642085
1104	70-71	0.4358591648990142
1104	72-73	0.41905268852251254
1104	74-75	0.4577288134726416
1104	76-77	0.39316080257567165
1104	78-79	0.37518266253528765
1104	80-81	0.39428290599778393
1104	82-83	0.39642572144548893
1104	84-85	0.43204332230325093
1104	86-87	0.39860286563010305
1104	88-89	0.3614340449404523
1104	90-91	0.4309135337622507
1104	92-93	0.4043883097126937
1104	94-95	0.4649177121315162
1104	96-97	0.43783760034266805
1104	98-99	0.3822396528439036
1104	100	0.4946037596815742
1105	1	0.1280093565942373
1105	2	0.2379855653706251
1105	3	0.2356714005377043
1105	4	0.26975237197930824
1105	5	0.19073834580395044
1105	6	0.2091584089102696
1105	7	0.24405036059624763
1105	8	0.24221730518801365
1105	9	0.3295747106578375
1105	10-11	0.34302580211950584
1105	12-13	0.27646291592277095
1105	14-15	0.4079625753513483
1105	16-17	0.34133235140859597
1105	18-19	0.4344471439423785
1105	20-21	0.34473430073877864
1105	22-23	0.3610217360720256
1105	24-25	0.38115757955308993
1105	26-27	0.426660517287452
1105	28-29	0.45305904325628177
1105	30-31	0.49226827466269896
1105	32-33	0.3826430639685441
1105	34-35	0.4415813694515407
1105	36-37	0.4523516740072324
1105	38-39	0.40890451332553823
1105	40-41	0.4496747260312972
1105	42-43	0.4157047988010163
1105	44-45	0.3888070574103111
1105	46-47	0.3624796834678605
1105	48-49	0.27920620348682945
1105	50-51	0.3584629423263195
1105	52-53	0.4060364307849227
1105	54-55	0.3513131821238247
1105	56-57	0.42845597455974627
1105	58-59	0.39934364386088816
1105	60-61	0.4204290830266828
1105	62-63	0.40651651153861934
1105	64-65	0.4337541572141248
1105	66-67	0.41169033961982393
1105	68-69	0.42173895976957
1105	70-71	0.3573878869849949
1105	72-73	0.3152694319294582
1105	74-75	0.2936694341161683
1105	76-77	0.31070962254833745
1105	78-79	0.3032581391862763
1105	80-81	0.22406003936136898
1105	82-83	0.2471526407633391
1105	84-85	0.14932094400364093
1105	86-87	0.24107807525510339
1105	88-89	0.22867525556337398
1105	90-91	0.26490097853734795
1105	92-93	0.26488545199998725
1105	94-95	0.2555863240350149
1105	96-97	0.1429187704867516
1105	98-99	0.18770080899242103
1105	100	0.28452722959385923
1106	1	0.07425189528212073
1106	2	0.19032952930360736
1106	3	0.1594264854916716
1106	4	0.2716632957525107
1106	5	0.10874028107784994
1106	6	0.23859037551925155
1106	7	0.21146152451903788
1106	8	0.23868653637750015
1106	9	0.29205481410483003
1106	10-11	0.4136930794666469
1106	12-13	0.28997066858176623
1106	14-15	0.42543919805370933
1106	16-17	0.3366414405260443
1106	18-19	0.3645085102913441
1106	20-21	0.3098121172510844
1106	22-23	0.3466125258575019
1106	24-25	0.4241023515148825
1106	26-27	0.42979883507390326
1106	28-29	0.4012499358720447
1106	30-31	0.44838619029160043
1106	32-33	0.37237413832041
1106	34-35	0.35410082190375647
1106	36-37	0.3590675671508876
1106	38-39	0.4049986972140971
1106	40-41	0.37748439247977217
1106	42-43	0.30142565984037617
1106	44-45	0.33022148321146005
1106	46-47	0.29616027009650736
1106	48-49	0.23625823902695942
1106	50-51	0.27056252681556714
1106	52-53	0.20515660847886608
1106	54-55	0.356204876835875
1106	56-57	0.32773819876394583
1106	58-59	0.3088232934871584
1106	60-61	0.30015266464419454
1106	62-63	0.26273648655501347
1106	64-65	0.2851424581582691
1106	66-67	0.2559777204206597
1106	68-69	0.24731164867747424
1106	70-71	0.25347516636326617
1106	72-73	0.2183860474323609
1106	74-75	0.19639492251809543
1106	76-77	0.19944317950736234
1106	78-79	0.18737547798690457
1106	80-81	0.25065747825729545
1106	82-83	0.23311717068518334
1106	84-85	0.18687871481210294
1106	86-87	0.22509654716631644
1106	88-89	0.25326715208151995
1106	90-91	0.20739238225446144
1106	92-93	0.22671944746245742
1106	94-95	0.25031703472698297
1106	96-97	0.2204533793445691
1106	98-99	0.21914906856983762
1106	100	0.2210339395377261
1107	1	0.03355008578193264
1107	2	0.09113032980704361
1107	3	0.07455563153056843
1107	4	0.2201220988889716
1107	5	0.05665575637181064
1107	6	0.1306707610561233
1107	7	0.07226388958881103
1107	8	0.0993749010046372
1107	9	0.14938363148577594
1107	10-11	0.21035054564156752
1107	12-13	0.14626543634515343
1107	14-15	0.2678141528124911
1107	16-17	0.16921285233426175
1107	18-19	0.07487209743595713
1107	20-21	0.14673039935724574
1107	22-23	0.24393102130912325
1107	24-25	0.3010063198195425
1107	26-27	0.17106483536404227
1107	28-29	0.19320670952831875
1107	30-31	0.29709308983109395
1107	32-33	0.19660547702970632
1107	34-35	0.21626747891566822
1107	36-37	0.20572849294835294
1107	38-39	0.24587824714381412
1107	40-41	0.17279607167039046
1107	42-43	0.23450331530801805
1107	44-45	0.2124237180162254
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2216	90-91	-1.225324619789383
2216	92-93	-1.1616480525656883
2216	94-95	-1.1971830794820093
2216	96-97	-1.1607415479225125
2216	98-99	-1.1584811313323087
2216	100	-0.8082350103817966
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	2.0
2	14.0
3	17.0
4	49.0
5	25.0
6	61.0
7	202.0
8	525.0
9	907.0
10	1143.0
11	1911.0
12	3144.0
13	4881.0
14	6751.0
15	9905.0
16	12561.0
17	15340.0
18	17665.0
19	20263.0
20	23649.0
21	27702.0
22	32473.0
23	38238.0
24	45419.0
25	52431.0
26	57898.0
27	63625.0
28	73457.0
29	89921.0
30	104866.0
31	121679.0
32	150315.0
33	197552.0
34	295346.0
35	429827.0
36	701758.0
37	1249029.0
38	939354.0
39	407681.0
40	605.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.93388802811357	21.588937981635553	11.566587339307235	23.910586650943642
2	15.859597238687465	20.077251646748692	40.06123338677884	24.001917727785006
3	18.23892631394659	27.88763312135242	28.892790115808726	24.980650448892273
4	11.754134470553627	16.668459125117383	41.437130978818914	30.140275425510076
5	12.535898532969705	39.499986918005135	35.408189020974284	12.555925528050876
6	28.64561830928527	39.73788692785888	18.357303164140788	13.25919159871506
7	26.120092956326683	32.148653172096175	24.715186585971136	17.016067285606002
8	26.660490073428484	34.62033093124647	19.91528089295378	18.80389810237126
9	26.52829863455688	14.245658949714187	21.905668268385426	37.32037414734351
10-11	25.878927525902157	25.88107251257294	27.45855149915812	20.781448462366786
12-13	25.74109592203425	24.059363084771977	27.544727192979078	22.65481380021469
14-15	23.270361305233504	25.011951296039904	24.88601301108984	26.83167438763675
16-17	21.75190752501485	27.51256814306979	26.422808059578745	24.31271627233662
18-19	20.90962353310768	28.791964920303194	28.19883547980404	22.099576066785087
20-21	23.11508022390321	27.783751069373096	26.814971665821176	22.286197040902522
22-23	23.90941466213016	27.339189161632483	26.375409137831596	22.375987038405764
24-25	23.155578008121783	27.342756100812622	27.59217324574524	21.909492645320356
26-27	23.08745597824393	27.494142937338566	26.17003700270758	23.24836408170992
28-29	21.831017414767505	28.277702839385388	26.87901352325094	23.01226622259617
30-31	22.850210132331906	27.91960815057576	26.903797003221484	22.32638471387085
32-33	23.628584384455216	28.05914896883505	25.552280275398005	22.759986371311726
34-35	23.31912661994624	27.701148054294606	27.090549233052947	21.88917609270621
36-37	22.83986203652444	27.205364241564023	26.58739522514779	23.367378496763745
38-39	23.200340063222207	27.312640574759943	27.354061491110667	22.132957870907184
40-41	22.511275285102194	26.152091442018687	28.236569073542196	23.10006419933692
42-43	22.589254577629582	26.51920254703173	27.247669397667785	23.643873477670905
44-45	22.736374375722818	27.842346242205164	27.163363180367245	22.257916201704774
46-47	22.736541739943586	27.525523456951657	26.48304465113773	23.25489015196703
48-49	23.71336644979846	26.952400725647735	26.71820846286289	22.616024361690908
50-51	23.083391810457538	26.57751142011576	26.823750300402775	23.515346469023928
52-53	22.29714394241953	27.581402244810132	26.590115534821557	23.531338277948784
54-55	22.319801137582616	27.48890211405804	26.494311028655837	23.696985719703505
56-57	23.929323457565445	26.544464428918634	26.203012755687112	23.32319935782881
58-59	22.368059145198796	27.212781159859066	27.46356794712428	22.95559174781786
60-61	22.381899526150242	28.144510947704322	26.35629211002396	23.117297416121474
62-63	22.976953639173246	27.122712773433292	25.839079026744805	24.061254560648653
64-65	21.825317499514675	27.560799120049822	26.491202518409096	24.122680862026407
66-67	22.91658429464489	26.840061095268425	26.43549782475332	23.807856785333364
68-69	22.048774087475845	27.015603502450553	26.923883831240808	24.01173857883279
70-71	22.160399121498667	26.663973816958624	26.977169104388267	24.198457957154446
72-73	23.01311937338836	26.73729104592329	27.5892490052875	22.660340575400852
74-75	23.290885714656767	27.079089172706734	26.886066743529323	22.743958369107176
76-77	22.875872888922153	27.078582152590663	26.46020196007339	23.58534299841379
78-79	22.65028736220991	27.04054201083225	26.517871117546214	23.791299509411626
80-81	22.094660743576224	27.56028258678556	27.4104545259089	22.934602143729315
82-83	23.226729228485148	26.14900731466528	28.798989889176198	21.825273567673374
84-85	23.519746129641998	27.010290395774906	26.867907742659202	22.602055731923894
86-87	21.743312894766714	27.94620302480525	27.457245430181487	22.853238650246553
88-89	23.39009209450574	26.266206865901907	27.058606128006907	23.285094911585446
90-91	22.78289269574963	26.356032602291013	27.36306725124399	23.49800745071537
92-93	23.365724122440167	26.64721904641889	27.182578523574506	22.804478307566438
94-95	21.755022603785214	26.830398695448576	28.063381406217474	23.351197294548733
96-97	22.28947963827022	26.684074393019053	27.404153612795835	23.622292355914894
98-99	21.388579217168488	27.80811005465197	27.109013520092557	23.694297208086986
100	22.59737088390488	26.781149771507007	25.919469238894195	24.702010105693923
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	749.0
1	511.0
2	354.5
3	421.5
4	403.0
5	378.0
6	365.0
7	364.0
8	376.0
9	405.5
10	341.0
11	303.0
12	342.5
13	364.5
14	385.5
15	682.5
16	879.0
17	955.0
18	1353.0
19	1670.0
20	1937.0
21	2376.5
22	3165.0
23	4289.5
24	5566.5
25	7092.0
26	9630.5
27	11977.0
28	16273.5
29	27795.0
30	45376.0
31	61493.5
32	82705.5
33	101797.5
34	112794.0
35	128041.5
36	141805.0
37	168284.0
38	176856.0
39	158435.5
40	140785.0
41	122029.0
42	116534.0
43	126291.0
44	142545.5
45	182102.5
46	221085.0
47	244383.5
48	258215.0
49	249503.5
50	234458.0
51	245262.5
52	253265.0
53	240360.0
54	226258.0
55	189729.5
56	163697.5
57	131358.5
58	96618.0
59	98447.5
60	81676.5
61	50307.0
62	36020.0
63	31952.5
64	23007.0
65	7605.0
66	2277.5
67	1237.0
68	787.5
69	458.0
70	235.5
71	147.5
72	105.5
73	70.5
74	58.0
75	53.5
76	41.0
77	30.5
78	27.0
79	23.0
80	16.0
81	16.5
82	17.5
83	14.5
84	13.5
85	9.0
86	7.0
87	11.0
88	8.5
89	6.0
90	7.0
91	11.0
92	13.0
93	8.0
94	7.5
95	6.5
96	5.5
97	7.0
98	5.5
99	4.5
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06275259989484804
2	0.00717557319459789
3	0.007618034812495347
4	0.009387881284085176
5	0.0039821545610771135
6	0.0024816325525553024
7	9.811105440334915E-4
8	0.0013850972386355177
9	4.809365411928881E-4
10-11	4.520803487213148E-4
12-13	2.1161207812487074E-4
14-15	1.9237461647715522E-4
16-17	5.482676569598925E-4
18-19	0.0016255655092319615
20-21	0.0010003480056812073
22-23	0.0012504350071015089
24-25	0.0013850972386355177
26-27	0.0037128300980090955
28-29	0.004184147908378126
30-31	0.005309539414769484
32-33	0.006386837267041553
34-35	0.0034435056349410784
36-37	0.008435626932523256
38-39	0.006425312190336984
40-41	0.006117512803973536
42-43	0.01478398927626938
44-45	0.020786077310356622
46-47	0.019449073725840392
48-49	0.022680967282656603
50-51	0.01927593657101095
52-53	0.026153329110069252
54-55	0.01981458549714699
56-57	0.015832430936069876
58-59	0.018227494911210458
60-61	0.01670773544104093
62-63	0.011590570642748602
64-65	0.012004176068174485
66-67	0.014129915580247052
68-69	0.005530770223718212
70-71	0.009147413013488731
72-73	0.00881075743465371
74-75	0.011705995412634895
76-77	0.011148109024851145
78-79	0.012629393571725241
80-81	0.010898022023430844
82-83	0.006646542999285712
84-85	0.011109634101555714
86-87	0.009637968285505475
88-89	0.008320202162636963
90-91	0.01353355426916787
92-93	0.009012750781954723
94-95	0.007483372580961338
96-97	0.009599493362210046
98-99	0.009282075245022739
100	0.2652268837370539
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5198191.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.45280515980096
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.07636498101164	44.2929481380481
2	15.252306546652886	18.745940448980328
3	5.887808793416034	10.854670997998749
4	2.8417848033577626	6.985425913073115
5	1.4516128353345048	4.460284036863879
6	0.8058523708015457	2.971313323825864
7	0.5250669652529097	2.258678653807453
8	0.3120792715428941	1.5342517334830472
9	0.22297633266427835	1.2332269013818375
>10	0.6149741586986055	5.355786602083157
>50	0.006237526111987809	0.26263444905522365
>100	0.0024336263310233463	0.28987291668937243
>500	2.007155310873238E-4	0.07104690671793806
>1k	2.5089441069948884E-4	0.3868417682902451
>5k	5.017888213989776E-5	0.29707720970173146
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	9634	0.18533370551409134	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	1.9237461647715524E-5	0.0
16-17	0.0	0.0	0.0	1.9237461647715524E-5	0.0
18-19	0.0	0.0	0.0	1.9237461647715524E-5	0.0
20-21	0.0	0.0	0.0	4.8093654119288806E-5	0.0
22-23	0.0	0.0	0.0	1.3466223153400865E-4	0.0
24-25	0.0	0.0	0.0	2.5970573224415956E-4	0.0
26-27	0.0	0.0	0.0	6.348362343746123E-4	0.0
28-29	0.0	0.0	0.0	0.0035204554815319406	0.0
30-31	0.0	0.0	0.0	0.013302704729395286	0.0
32-33	0.0	0.0	0.0	0.0290581858188743	0.0
34-35	0.0	0.0	0.0	0.05409574215337605	0.0
36-37	0.0	0.0	0.0	0.09802448582593445	0.0
38-39	0.0	0.0	0.0	0.16419173516325197	0.0
40-41	0.0	0.0	0.0	0.23695743384573595	0.0
42-43	0.0	0.0	0.0	0.3123586647739569	0.0
44-45	0.0	0.0	0.0	0.40264007228668586	0.0
46-47	0.0	0.0	0.0	0.5181129358270983	0.0
48-49	0.0	0.0	0.0	0.6531214416707658	0.0
50-51	0.0	0.0	0.0	0.7994031000399947	0.0
52-53	0.0	0.0	0.0	0.9418565035413281	0.0
54-55	0.0	0.0	0.0	1.0950155544496152	0.0
56-57	0.0	0.0	0.0	1.285322143799641	0.0
58-59	0.0	0.0	0.0	1.5192592961666858	0.0
60-61	0.0	0.0	0.0	1.7646715944065927	0.0
62-63	0.0	0.0	0.0	2.0200296603183685	0.0
64-65	0.0	0.0	0.0	2.283736784585253	0.0
66-67	0.0	0.0	0.0	2.582994737977115	0.0
68-69	0.0	0.0	0.0	2.921708725208443	0.0
70-71	0.0	0.0	0.0	3.2969738895704293	0.0
72-73	0.0	0.0	0.0	3.692611525817347	0.0
74-75	0.0	0.0	0.0	4.072224356511717	0.0
76-77	0.0	0.0	0.0	4.511531030698949	0.0
78-79	0.0	0.0	0.0	4.985917216200789	0.0
80-81	0.0	0.0	0.0	5.4947384580520415	0.0
82-83	0.0	0.0	0.0	6.030578714787509	0.0
84-85	0.0	0.0	0.0	6.549836279582648	0.0
86-87	0.0	0.0	0.0	7.099508271242823	0.0
88	0.0	0.0	0.0	7.5467215421672655	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	6445	0.0	47.659855	94
GTACTAG	7900	0.0	36.547134	1
TATTCTC	2760	0.0	34.732533	5
GCATCGT	7870	0.0	34.51021	8
TAGGCAT	9110	0.0	34.250355	5
TACCCCG	335	0.0	33.665245	5
CTGTGCG	9675	0.0	33.171432	9
TAGTACT	9835	0.0	33.017494	4
ACTGTGC	9925	0.0	32.904007	8
TACCTGG	1330	0.0	32.861263	2
CAACGCA	10125	0.0	32.534065	5
TACTGTG	11215	0.0	31.004658	7
CTAGGCA	10430	0.0	30.72844	4
GTATCAA	12320	0.0	30.534538	1
GTACTGT	10520	0.0	30.46233	6
ATCAACG	11005	0.0	29.720741	3
AGGGCTA	6925	0.0	29.042856	5
CAGTACG	875	0.0	28.99881	9
CATGGGT	995	0.0	28.81026	4
TCTCTGC	3270	0.0	28.45194	8
>>END_MODULE