FastQCFastQC Report
Mon 16 Jan 2017
ERR1146640_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146640_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5198191
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169610.32628658700690294No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140430.2701516739188691No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92810.17854288155244777No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC64850.048.0756694
GGTATCA119050.043.586061
GTGGTAT21150.041.1493381
GTATCAA211800.040.7578851
GTACTAG73800.036.398311
CAGTACG7950.036.0665639
CAACGCA237800.034.4882625
TACCCCG3450.034.0571755
CTGTGCG96850.034.022059
ATCAACG243350.033.7118953
TAGTACT97900.033.490234
ACTGTGC99800.033.152268
TAGGCAT86850.033.010175
CTAACGC37150.032.541863
TCTAACG38000.032.1877982
TAACGCC38100.031.7304484
GTACTGT104050.031.7077966
TATCAAC272400.031.621522
TACTGTG112750.031.5537037
TATTCTC32750.031.2847655
Produced by FastQC (version 0.11.5)