##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146640_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5198191
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16803980461665	33.0	31.0	34.0	30.0	34.0
2	32.23823576317223	33.0	31.0	34.0	30.0	34.0
3	32.38443123771327	34.0	31.0	34.0	30.0	34.0
4	35.984252983393645	37.0	35.0	37.0	35.0	37.0
5	35.90228927717354	37.0	35.0	37.0	35.0	37.0
6	35.9350343225172	37.0	35.0	37.0	35.0	37.0
7	35.863573693232894	37.0	35.0	37.0	35.0	37.0
8	35.87499285809236	37.0	35.0	37.0	35.0	37.0
9	37.58934329269548	39.0	37.0	39.0	35.0	39.0
10-11	37.52132751566843	39.0	37.0	39.0	35.0	39.0
12-13	37.47519069614795	39.0	37.0	39.0	34.5	39.0
14-15	38.6864838171587	40.0	38.0	41.0	34.0	41.0
16-17	38.697528140077964	40.0	38.0	41.0	34.0	41.0
18-19	38.76125684877681	40.0	38.0	41.0	34.0	41.0
20-21	38.762092620298105	40.0	38.0	41.0	34.0	41.0
22-23	38.71737860344108	40.0	38.0	41.0	34.0	41.0
24-25	38.62911462853135	40.0	38.0	41.0	34.0	41.0
26-27	38.47856638973059	40.0	38.0	41.0	34.0	41.0
28-29	38.424471320888365	40.0	38.0	41.0	34.0	41.0
30-31	38.276846022010346	40.0	37.5	41.0	34.0	41.0
32-33	38.11105757753034	40.0	37.0	41.0	33.5	41.0
34-35	37.99731166092204	40.0	37.0	41.0	33.0	41.0
36-37	37.9731236308939	40.0	37.0	41.0	33.0	41.0
38-39	37.85820721093165	40.0	37.0	41.0	33.0	41.0
40-41	37.72266630833688	40.0	36.5	41.0	33.0	41.0
42-43	37.569635090361245	40.0	36.0	41.0	32.5	41.0
44-45	37.312617408633116	40.0	36.0	41.0	31.5	41.0
46-47	37.01722185660358	39.5	35.0	41.0	31.0	41.0
48-49	36.68870391642015	39.0	35.0	41.0	30.5	41.0
50-51	36.436628146214716	39.0	35.0	41.0	30.0	41.0
52-53	36.225372634441484	39.0	35.0	41.0	30.0	41.0
54-55	36.0171497161224	38.0	35.0	40.5	30.0	41.0
56-57	35.756237506470995	37.5	35.0	40.0	29.0	41.0
58-59	35.43284663837862	37.0	35.0	40.0	29.0	41.0
60-61	35.238957071796705	36.5	34.0	40.0	29.0	41.0
62-63	35.01051096429508	36.0	34.0	39.5	29.0	41.0
64-65	34.757662521442555	35.5	34.0	39.0	28.0	41.0
66-67	34.515360055065315	35.0	34.0	39.0	28.0	41.0
68-69	34.285940435817	35.0	34.0	38.5	28.0	41.0
70-71	34.11235264344846	35.0	34.0	37.5	28.0	41.0
72-73	33.90641494319851	35.0	34.0	37.0	28.0	40.0
74-75	33.67983044486053	35.0	34.0	36.5	27.5	39.5
76-77	33.46629394725973	35.0	33.5	36.0	27.0	39.0
78-79	33.270259980827944	35.0	33.0	36.0	27.0	39.0
80-81	33.17553702816999	35.0	33.0	36.0	27.0	38.5
82-83	33.027595753984414	35.0	33.0	35.0	27.0	37.0
84-85	32.91838747364227	35.0	33.0	35.0	27.0	37.0
86-87	32.7930052012325	35.0	33.0	35.0	27.0	36.5
88-89	32.70368460874177	35.0	33.0	35.0	27.0	36.0
90-91	32.644572409901826	35.0	33.0	35.0	27.0	36.0
92-93	32.57139839609587	35.0	33.0	35.0	27.0	36.0
94-95	32.53376895923986	35.0	33.0	35.0	27.0	35.5
96-97	32.449025728373584	35.0	33.0	35.0	27.0	35.0
98-99	32.385899729348154	35.0	33.0	35.0	27.0	35.0
100	31.16782165180156	34.0	31.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.38707502478944633
1101	2	0.4541825988732455
1101	3	0.356215940716865
1101	4	0.2838780394739828
1101	5	0.2942893370947033
1101	6	0.2801930058720572
1101	7	0.3509762076775331
1101	8	0.2997700120221012
1101	9	0.3565380329601098
1101	10-11	0.41022674673018145
1101	12-13	0.4148950601738619
1101	14-15	0.5573807509978579
1101	16-17	0.5664584899632246
1101	18-19	0.5542366574826261
1101	20-21	0.6028646910369702
1101	22-23	0.5608543214551176
1101	24-25	0.521319887977377
1101	26-27	0.6305713703310545
1101	28-29	0.6589378038980271
1101	30-31	0.6830581325246428
1101	32-33	0.6643138057734319
1101	34-35	0.6687784004732293
1101	36-37	0.573195036365945
1101	38-39	0.5795224376844743
1101	40-41	0.6338386046928619
1101	42-43	0.612712841504738
1101	44-45	0.5930906391634423
1101	46-47	0.5697798350364636
1101	48-49	0.49920388688384776
1101	50-51	0.5852504501016824
1101	52-53	0.6017987914616896
1101	54-55	0.6239913905009189
1101	56-57	0.603430986714919
1101	58-59	0.5835845500194594
1101	60-61	0.6402321365415844
1101	62-63	0.5769497352358286
1101	64-65	0.5726692157013602
1101	66-67	0.48650311967431037
1101	68-69	0.5048359030649081
1101	70-71	0.48664620070847064
1101	72-73	0.5323048394330172
1101	74-75	0.5935944984704165
1101	76-77	0.5298806344049751
1101	78-79	0.4759576111336088
1101	80-81	0.5022659862661953
1101	82-83	0.49105771327934633
1101	84-85	0.5154650447172315
1101	86-87	0.4834219523191692
1101	88-89	0.48592543675891164
1101	90-91	0.46875062377110055
1101	92-93	0.4446537451658443
1101	94-95	0.48919899928281296
1101	96-97	0.5035783694102989
1101	98-99	0.559635819690925
1101	100	0.8651310909665675
1102	1	0.32852135998197696
1102	2	0.3895460773024695
1102	3	0.3104484902099145
1102	4	0.19124376149552802
1102	5	0.20035252751454635
1102	6	0.2102191339621413
1102	7	0.23056614696096744
1102	8	0.24522511022890114
1102	9	0.34106975197562406
1102	10-11	0.29192349942340456
1102	12-13	0.3391903928683604
1102	14-15	0.4664557684613726
1102	16-17	0.4390073382620727
1102	18-19	0.4585945435499852
1102	20-21	0.44307910509211723
1102	22-23	0.49061795586230517
1102	24-25	0.4975539046640236
1102	26-27	0.4940966289454707
1102	28-29	0.5073493521000074
1102	30-31	0.544522243429725
1102	32-33	0.5300121898858379
1102	34-35	0.5466061691974673
1102	36-37	0.5610536693943402
1102	38-39	0.5517998846250762
1102	40-41	0.5240571971187009
1102	42-43	0.5168300530572552
1102	44-45	0.5143708719390503
1102	46-47	0.5595325900428065
1102	48-49	0.6025010104592923
1102	50-51	0.55200976580457
1102	52-53	0.5189217726931687
1102	54-55	0.5193158955321806
1102	56-57	0.5000118848987896
1102	58-59	0.4863330906171299
1102	60-61	0.49765179191000186
1102	62-63	0.5164837930843333
1102	64-65	0.42501146884394103
1102	66-67	0.425383275239156
1102	68-69	0.4322037109989125
1102	70-71	0.36827728679719485
1102	72-73	0.40889280666367966
1102	74-75	0.43563132217121137
1102	76-77	0.4145211747028483
1102	78-79	0.39209768430127667
1102	80-81	0.3686724569295734
1102	82-83	0.36190493011900315
1102	84-85	0.3397529843502838
1102	86-87	0.3734535453835335
1102	88-89	0.346269678864104
1102	90-91	0.3508414033652514
1102	92-93	0.3249022414887932
1102	94-95	0.40310283500891586
1102	96-97	0.3719457468884855
1102	98-99	0.3888265900228376
1102	100	0.6497339731986607
1103	1	0.24902690064015331
1103	2	0.33269878155292787
1103	3	0.23620831457726865
1103	4	0.16665605393500726
1103	5	0.16454330122630267
1103	6	0.20805374598438675
1103	7	0.18492133189399595
1103	8	0.16532313918251873
1103	9	0.24787698564524874
1103	10-11	0.23243052952864218
1103	12-13	0.2465005000635756
1103	14-15	0.4022388075030747
1103	16-17	0.32617923972584606
1103	18-19	0.3135160597507465
1103	20-21	0.34398827470515414
1103	22-23	0.30291134723843527
1103	24-25	0.32612753404173134
1103	26-27	0.3403035973865798
1103	28-29	0.38581469853182426
1103	30-31	0.3398795505990364
1103	32-33	0.32802823050457874
1103	34-35	0.3211337597050843
1103	36-37	0.28690105070671734
1103	38-39	0.24840458474271543
1103	40-41	0.29271146038455953
1103	42-43	0.30475396994827264
1103	44-45	0.3248547187989672
1103	46-47	0.2944594119697257
1103	48-49	0.24883092963050046
1103	50-51	0.2942472693193423
1103	52-53	0.2605894895530696
1103	54-55	0.2394223218171021
1103	56-57	0.197081138605796
1103	58-59	0.2294618780975881
1103	60-61	0.3051864577246661
1103	62-63	0.30774511180275965
1103	64-65	0.2887576066630899
1103	66-67	0.290345255955927
1103	68-69	0.29720055265224943
1103	70-71	0.32862445259493
1103	72-73	0.3040908427032747
1103	74-75	0.2641404317222822
1103	76-77	0.1955805667747228
1103	78-79	0.2705246927342131
1103	80-81	0.23245677018367417
1103	82-83	0.22221767209657628
1103	84-85	0.19156059706485706
1103	86-87	0.19248764203470614
1103	88-89	0.23285110289435096
1103	90-91	0.21066093534109598
1103	92-93	0.17496130729480086
1103	94-95	0.21949281090375194
1103	96-97	0.25631157777353053
1103	98-99	0.21473138892336863
1103	100	0.4839843990304189
1104	1	0.24875999787906267
1104	2	0.27710381427544206
1104	3	0.23716313768066755
1104	4	0.20243826033656376
1104	5	0.19942590520324188
1104	6	0.21275753152652044
1104	7	0.21777420874448694
1104	8	0.24438235371250983
1104	9	0.3047905639830475
1104	10-11	0.30261033150266314
1104	12-13	0.28025692970213356
1104	14-15	0.3092849661156336
1104	16-17	0.3229662690147208
1104	18-19	0.34129791919559693
1104	20-21	0.3526446183405838
1104	22-23	0.3999274452733559
1104	24-25	0.40067430242833524
1104	26-27	0.4183165058237819
1104	28-29	0.43560323929052913
1104	30-31	0.40398787186068574
1104	32-33	0.41237736997361196
1104	34-35	0.42740220824001796
1104	36-37	0.3776096844590029
1104	38-39	0.3776707409188873
1104	40-41	0.48337835196308276
1104	42-43	0.49664305164341016
1104	44-45	0.42132561603252583
1104	46-47	0.4190926950040179
1104	48-49	0.3902602548598111
1104	50-51	0.48598520740789297
1104	52-53	0.4266288953400803
1104	54-55	0.4523349592255954
1104	56-57	0.4251822650738788
1104	58-59	0.4773332652707438
1104	60-61	0.43454922054538514
1104	62-63	0.39102331449407757
1104	64-65	0.3706908794196764
1104	66-67	0.34746871845422334
1104	68-69	0.30428504836033454
1104	70-71	0.31398742751977693
1104	72-73	0.30351457921498337
1104	74-75	0.35503941728245536
1104	76-77	0.27451245805207236
1104	78-79	0.35838493718134856
1104	80-81	0.3105048520492488
1104	82-83	0.31669042822936433
1104	84-85	0.2537139104676598
1104	86-87	0.3157749906615024
1104	88-89	0.2860801232231225
1104	90-91	0.2755564509290309
1104	92-93	0.27053266963132216
1104	94-95	0.2557132544194971
1104	96-97	0.2541815215446661
1104	98-99	0.273561197407048
1104	100	0.4231675408510327
1105	1	0.21173574073358026
1105	2	0.21405218216816735
1105	3	0.15629564247493732
1105	4	0.18450901742652803
1105	5	0.1510654317667104
1105	6	0.14063757064829474
1105	7	0.20693542774280616
1105	8	0.1701955458656741
1105	9	0.18586610780177182
1105	10-11	0.20596214681334146
1105	12-13	0.17140854097370095
1105	14-15	0.2912182521117117
1105	16-17	0.2817494005250225
1105	18-19	0.2781261905038761
1105	20-21	0.31644448523881863
1105	22-23	0.2779526660701279
1105	24-25	0.3363133279753825
1105	26-27	0.3460019984168241
1105	28-29	0.300649890019713
1105	30-31	0.33705351666402805
1105	32-33	0.27537305753126873
1105	34-35	0.2279600002448703
1105	36-37	0.3615932482416113
1105	38-39	0.31692082008085265
1105	40-41	0.2821874955471273
1105	42-43	0.2618573047039945
1105	44-45	0.25171412993967124
1105	46-47	0.276129397027411
1105	48-49	0.2664727009028738
1105	50-51	0.29198588148402393
1105	52-53	0.25557300322241616
1105	54-55	0.21557550057007546
1105	56-57	0.182263302938658
1105	58-59	0.2468288955987603
1105	60-61	0.20476366551376657
1105	62-63	0.21687351743657501
1105	64-65	0.1676358786451928
1105	66-67	0.16509557807503938
1105	68-69	0.14720650358344756
1105	70-71	0.17927183830116178
1105	72-73	0.14320195113366196
1105	74-75	0.2069185116010246
1105	76-77	0.1314712232350672
1105	78-79	0.09226143526159092
1105	80-81	0.03178561898133836
1105	82-83	0.1500099224974889
1105	84-85	0.1318769793200616
1105	86-87	0.16801703916757305
1105	88-89	0.15302784342704
1105	90-91	0.16288973959811415
1105	92-93	0.16801715251520477
1105	94-95	0.13306552996619558
1105	96-97	0.06678277004196076
1105	98-99	0.13460081334471852
1105	100	0.25863878415310637
1106	1	0.1785805839861112
1106	2	0.22353308030418617
1106	3	0.18949884589957122
1106	4	0.10414353421303701
1106	5	0.05494772729516484
1106	6	0.12631005821933883
1106	7	0.15834785257665374
1106	8	0.12502323978939955
1106	9	0.16647779043427846
1106	10-11	0.15553237456497726
1106	12-13	0.12851468977527958
1106	14-15	0.2331005338981882
1106	16-17	0.19181082217710355
1106	18-19	0.20007521812426177
1106	20-21	0.229783387428391
1106	22-23	0.2255546559097752
1106	24-25	0.2094551181272024
1106	26-27	0.251797237120158
1106	28-29	0.27125371123043607
1106	30-31	0.28691512480491355
1106	32-33	0.27885706169140434
1106	34-35	0.25877810594889894
1106	36-37	0.19619556185585907
1106	38-39	0.28345886510562934
1106	40-41	0.24471993585243723
1106	42-43	0.2182267824783466
1106	44-45	0.2149305692434993
1106	46-47	0.2602548050554745
1106	48-49	0.33445284172136525
1106	50-51	0.3199043693828685
1106	52-53	0.341218769333139
1106	54-55	0.28505965153484425
1106	56-57	0.2785888298181547
1106	58-59	0.16120210013280456
1106	60-61	0.17861643588148723
1106	62-63	0.23722223207484205
1106	64-65	0.24764908100734573
1106	66-67	0.25641510875603046
1106	68-69	0.2506194348597717
1106	70-71	0.20229540547282454
1106	72-73	0.14895392771731508
1106	74-75	0.19287265124038555
1106	76-77	0.22410681725924064
1106	78-79	0.23447290465868775
1106	80-81	0.1628604819720607
1106	82-83	0.16521214588387068
1106	84-85	0.20010361283166844
1106	86-87	0.1650353632686361
1106	88-89	0.2186251405458819
1106	90-91	0.15768760168018048
1106	92-93	0.15368258474927643
1106	94-95	0.20226953712946028
1106	96-97	0.11087286954399644
1106	98-99	0.11864180870696828
1106	100	0.3029908444623288
1107	1	0.09938994751953345
1107	2	0.14649873559754667
1107	3	0.15535156673489325
1107	4	0.10897889826435403
1107	5	0.0240680275928824
1107	6	0.02434328445909273
1107	7	0.10875530823035717
1107	8	0.09242864417780794
1107	9	0.033929809987398585
1107	10-11	0.10887367060023934
1107	12-13	0.030637233195704994
1107	14-15	0.10729306170760111
1107	16-17	0.13165089478089698
1107	18-19	0.10361329798546848
1107	20-21	0.13525681356625086
1107	22-23	0.060887576811538224
1107	24-25	0.050286456143346925
1107	26-27	0.13537020037413328
1107	28-29	0.13456461209882065
1107	30-31	0.12104181952484794
1107	32-33	0.12977972879178878
1107	34-35	0.1469468590340952
1107	36-37	0.09040005661729111
1107	38-39	0.13765861170530513
1107	40-41	0.17121220907325352
1107	42-43	0.1403260777303288
1107	44-45	0.1449133169289496
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2216	56-57	-0.7880320870449893
2216	58-59	-0.9250182378066185
2216	60-61	-0.8472069612698476
2216	62-63	-0.8328404305902097
2216	64-65	-0.8070236721708071
2216	66-67	-0.8673671585936376
2216	68-69	-0.8481670367048366
2216	70-71	-0.8660863160571921
2216	72-73	-0.885828502504296
2216	74-75	-0.8494727313067543
2216	76-77	-0.8381784615662724
2216	78-79	-0.7741029489790208
2216	80-81	-0.8623683663577424
2216	82-83	-0.810982976697133
2216	84-85	-0.8126220823369223
2216	86-87	-0.8679067297948073
2216	88-89	-0.7882222676383677
2216	90-91	-0.8083895976373441
2216	92-93	-0.8236417998960022
2216	94-95	-0.881807313271338
2216	96-97	-0.8591069667763129
2216	98-99	-0.8707029892225719
2216	100	-0.29050775093263326
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	16.0
13	96.0
14	320.0
15	968.0
16	2421.0
17	5150.0
18	9201.0
19	14491.0
20	20750.0
21	29392.0
22	40397.0
23	47486.0
24	47800.0
25	44400.0
26	42896.0
27	49756.0
28	62149.0
29	77836.0
30	96814.0
31	120975.0
32	153743.0
33	200050.0
34	289069.0
35	428353.0
36	695576.0
37	1306027.0
38	981241.0
39	430036.0
40	777.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.34309243350235	22.37934312148207	12.75360986158454	24.523954583431042
2	14.557924219098407	20.891193037634956	39.72448590492776	24.826396838338873
3	17.085636137648656	28.24940445628104	29.707296249791515	24.95766315627879
4	11.306427196348023	17.386321494217153	40.17639871053259	31.130852598902237
5	11.89523801095585	39.57601394702608	35.42474596735562	13.104002074662455
6	28.41257376608926	40.05495761215254	17.827558372263567	13.704910249494631
7	25.07505497105723	33.06725995009801	24.736725667972888	17.120959410871862
8	27.03761750962979	33.898504306594354	19.65541473947379	19.408463444302065
9	26.6464237270235	13.52605165912526	22.034049922367224	37.79347469148402
10-11	25.824156903815194	25.935474475639698	27.173674457133263	21.066694163411846
12-13	25.974787855609556	24.002401229894485	28.061859083279217	21.96095183121674
14-15	23.299067113442533	24.617077084998137	26.06562595324899	26.018229848310337
16-17	21.029989086588007	26.887045897313122	27.658737433849584	24.424227582249287
18-19	20.309719433991283	27.142426635740506	30.849150622375465	21.69870330789274
20-21	22.2502212789026	26.406859617124496	29.740432777479704	21.6024863264932
22-23	22.852046029089735	25.915563702834316	29.46715501604308	21.765235252032873
24-25	22.2268766191931	25.920401924438714	30.547453912332195	21.305267544035992
26-27	22.172905920540433	26.146855704224798	29.138040522173963	22.542197853060806
28-29	21.048755230425353	26.875003246321654	29.870708867758033	22.20553265549496
30-31	22.18905576959369	26.43820706087945	29.74900306664376	21.6237341028831
32-33	23.020325724853127	26.63685308985376	28.619619786960506	21.723201398332613
34-35	22.546612850508957	26.456598074214664	29.770087324609655	21.22670175066672
36-37	22.215795841284017	25.87526122068235	29.274424506525442	22.63451843150819
38-39	22.47455893790744	25.832621386940186	30.26687553420026	21.42594414095211
40-41	21.720989090243126	25.009479259226914	30.81152654837038	22.45800510215958
42-43	22.09483068244318	25.25984520384111	29.57840525675182	23.066918856963895
44-45	22.304345877248448	26.525170006257948	29.2846876923145	21.885796424179105
46-47	22.428379411222096	26.363354097608187	28.260013916379755	22.948252574789958
48-49	23.646630144986975	26.164881205788703	28.05070071492179	22.13778793430253
50-51	22.6723392811076	25.998053938379716	27.88385421005115	23.44575257046153
52-53	21.776335267403603	26.89615483540332	27.663094718912788	23.66441517828029
54-55	21.981579745723078	26.910534837984983	27.29897766357566	23.808907752716284
56-57	23.577182908438722	25.949921809337134	27.015455953811625	23.45743932841252
58-59	21.9497363505374	26.633597463732567	28.25867273031574	23.15799345541429
60-61	22.12823653459444	27.48940929642639	27.101168464183022	23.281185704796147
62-63	22.82037154848677	26.40957209921682	26.51064572271392	24.259410629582483
64-65	21.661259654325697	26.840804911249844	27.140486261751214	24.357449172673245
66-67	22.573179155145418	26.15196709543495	27.166032679294087	24.10882107012554
68-69	21.93223565659669	25.963917062685848	27.797199064059015	24.30664821665845
70-71	22.004672224331408	25.70180274140892	27.706846250500057	24.58667878375962
72-73	22.771007931920703	25.577936173157745	28.330662683729198	23.320393211192357
74-75	22.935931069548168	26.1526707395126	27.63432763895427	23.27707055198496
76-77	22.588104343383065	26.18192741579125	26.952831402819022	24.277136838006665
78-79	22.11845104915856	26.267568737153578	27.23247199818321	24.38150821550465
80-81	21.654764128520863	26.944200395868485	28.014851705141268	23.38618377046938
82-83	22.775480931731828	25.37787664978066	29.422649148521092	22.423993269966417
84-85	23.223675313200303	26.21894809174961	27.332816358613986	23.224560236436098
86-87	21.247584015285316	27.08678846160135	28.04427540273145	23.62135212038188
88-89	22.587146182200694	25.588709610708804	27.680668140127977	24.143476066962528
90-91	22.06443549303979	25.495763814757865	28.03003968111214	24.409761011090204
92-93	22.733052710067792	25.936590248415264	27.470537346549982	23.859819694966962
94-95	21.020726248804632	26.016493045369053	28.44116347398547	24.521617231840846
96-97	21.545678102247493	25.789981553198025	27.82969113678201	24.834649207772475
98-99	20.590759362247365	26.820724748282625	27.70041539450936	24.88810049496065
100	21.71578688405303	25.821291497699818	26.444105901163585	26.018815717083566
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	619.0
1	392.0
2	223.5
3	301.0
4	218.5
5	163.5
6	356.0
7	763.5
8	1207.0
9	1358.5
10	1105.5
11	845.0
12	822.5
13	918.0
14	1150.0
15	1847.0
16	2588.5
17	3398.5
18	4679.0
19	6139.0
20	7845.0
21	9512.0
22	11332.5
23	13182.0
24	14562.5
25	15830.0
26	17879.5
27	19310.5
28	22822.5
29	33295.0
30	49114.0
31	62950.0
32	82677.5
33	101040.5
34	109419.5
35	122979.0
36	137000.0
37	162021.0
38	171011.5
39	154439.5
40	136989.0
41	118620.5
42	112753.0
43	120868.0
44	136911.0
45	177391.5
46	216133.5
47	238641.0
48	253940.0
49	246135.5
50	231576.0
51	240073.5
52	246871.5
53	236421.0
54	227511.0
55	191723.0
56	161694.0
57	130786.0
58	97655.0
59	100618.0
60	83037.5
61	49453.0
62	34124.5
63	30117.5
64	21369.5
65	6404.5
66	1530.5
67	798.0
68	505.0
69	281.5
70	132.5
71	67.0
72	32.5
73	7.0
74	4.0
75	3.0
76	1.0
77	0.5
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.008022021507097373
3	0.0
4	0.09097395613204672
5	0.0045592784105085785
6	5.771238494314657E-5
7	1.923746164771552E-5
8	0.0
9	0.0
10-11	0.0
12-13	4.1360542542588375E-4
14-15	0.009743774324567913
16-17	0.0
18-19	4.8093654119288806E-5
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	1.923746164771552E-5
60-61	0.0
62-63	0.0
64-65	5.771238494314657E-5
66-67	1.2504350071015091E-4
68-69	0.0
70-71	4.713178103690303E-4
72-73	3.366555788350217E-4
74-75	4.6169907954517257E-4
76-77	1.5389969318172416E-4
78-79	2.8856192471573286E-5
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.06511880767751704
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5198191.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.287353434685805
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.85872104675113	40.71879964147107
2	16.529493964456307	19.269209136055416
3	6.425911375161081	11.236481023919453
4	2.947196056026967	6.871370326358236
5	1.5416337377204152	4.492887526867278
6	0.8861132850073064	3.09895189358348
7	0.5384852580638904	2.1970816389296584
8	0.3453887361541441	1.6105436269260833
9	0.2344628275106685	1.22995959249691
>10	0.6725465029754507	5.667913714932826
>50	0.010445476348828767	0.41507927171081466
>100	0.007757047329973172	0.8964681076282252
>500	0.001001401241591452	0.401763236165488
>1k	6.851692674641088E-4	0.6559142701562838
>5k	5.27053282664699E-5	0.2851173407016285
>10k+	1.054106565329398E-4	0.9524596520971114
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16961	0.32628658700690294	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14043	0.2701516739188691	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9281	0.17854288155244777	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	2.8856192471573282E-5	0.0
22-23	0.0	0.0	0.0	9.618730823857761E-5	0.0
24-25	0.0	0.0	0.0	2.212308089487285E-4	0.0
26-27	0.0	0.0	0.0	6.05980041903039E-4	0.0
28-29	0.0	0.0	0.0	0.0035300742123557985	0.0
30-31	0.0	0.0	0.0	0.013264229806099852	0.0
32-33	0.0	0.0	0.0	0.028961998510635717	0.0
34-35	0.0	0.0	0.0	0.053980317383489754	0.0
36-37	0.0	0.0	0.0	0.09812067313417303	0.0
38-39	0.0	0.0	0.0	0.16445144089549613	0.0
40-41	0.0	0.0	0.0	0.23747684531022425	0.0
42-43	0.0	0.0	0.0	0.3130031197391554	0.0
44-45	0.0	0.0	0.0	0.4036019453690717	0.0
46-47	0.0	0.0	0.0	0.5193056584492567	0.0
48-49	0.0	0.0	0.0	0.6545546325635206	0.0
50-51	0.0	0.0	0.0	0.8009901906259312	0.0
52-53	0.0	0.0	0.0	0.9437513935136281	0.0
54-55	0.0	0.0	0.0	1.0973721435014605	0.0
56-57	0.0	0.0	0.0	1.2880827195460882	0.0
58-59	0.0	0.0	0.0	1.5233761129592969	0.0
60-61	0.0	0.0	0.0	1.7698657090514758	0.0
62-63	0.0	0.0	0.0	2.0267146782409498	0.0
64-65	0.0	0.0	0.0	2.2921820302485996	0.0
66-67	0.0	0.0	0.0	2.592671181185916	0.0
68-69	0.0	0.0	0.0	2.9332992958511914	0.0
70-71	0.0	0.0	0.0	3.310449731454654	0.0
72-73	0.0	0.0	0.0	3.7083670069068257	0.0
74-75	0.0	0.0	0.0	4.090201764421508	0.0
76-77	0.0	0.0	0.0	4.533019275359447	0.0
78-79	0.0	0.0	0.0	5.010262223915975	0.0
80-81	0.0	0.0	0.0	5.523950543564098	0.0
82-83	0.0	0.0	0.0	6.064888727636211	0.0
84-85	0.0	0.0	0.0	6.588946039112453	0.0
86-87	0.0	0.0	0.0	7.144100707342227	0.0
88	0.0	0.0	0.0	7.595488507444224	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	6485	0.0	48.07566	94
GGTATCA	11905	0.0	43.58606	1
GTGGTAT	2115	0.0	41.149338	1
GTATCAA	21180	0.0	40.757885	1
GTACTAG	7380	0.0	36.39831	1
CAGTACG	795	0.0	36.066563	9
CAACGCA	23780	0.0	34.488262	5
TACCCCG	345	0.0	34.057175	5
CTGTGCG	9685	0.0	34.02205	9
ATCAACG	24335	0.0	33.711895	3
TAGTACT	9790	0.0	33.49023	4
ACTGTGC	9980	0.0	33.15226	8
TAGGCAT	8685	0.0	33.01017	5
CTAACGC	3715	0.0	32.54186	3
TCTAACG	3800	0.0	32.187798	2
TAACGCC	3810	0.0	31.730448	4
GTACTGT	10405	0.0	31.707796	6
TATCAAC	27240	0.0	31.62152	2
TACTGTG	11275	0.0	31.553703	7
TATTCTC	3275	0.0	31.284765	5
>>END_MODULE