FastQCFastQC Report
Mon 16 Jan 2017
ERR1146639_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146639_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4866852
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC75940.15603515372976207No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC56250.045.57383794
GTACTAG59150.036.7272071
CTGTGCG82600.033.622959
TAGGCAT72800.033.2436525
TAGTACT84400.033.185974
GCATCGT62150.032.361688
ACTGTGC88500.031.9124098
TACTGTG97550.031.3122657
GTACTGT90350.031.2069636
CAGTACG5750.031.0559029
CTAGGCA81900.030.52664
TCTAACG31050.029.8173272
TATTCTC27300.029.7794255
CTAGTAC97700.029.7268643
TACCCCG2600.028.9187495
ACGTTAA7550.028.0087668
CTAACGC32850.027.8968033
TAACGCC33200.027.8855274
CGCCTAC32900.027.8526887
CAACGCA105200.027.3381165
Produced by FastQC (version 0.11.5)