##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146639_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4866852
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.303698776950686	31.0	31.0	34.0	28.0	34.0
2	31.432401272937824	31.0	31.0	34.0	28.0	34.0
3	31.567659957607095	33.0	31.0	34.0	28.0	34.0
4	34.99110513325657	37.0	35.0	37.0	32.0	37.0
5	35.14553247150314	37.0	35.0	37.0	32.0	37.0
6	35.2227497363799	37.0	35.0	37.0	32.0	37.0
7	35.14008808979603	37.0	35.0	37.0	32.0	37.0
8	35.1170103385104	37.0	35.0	37.0	32.0	37.0
9	36.59669186570703	39.0	37.0	39.0	32.0	39.0
10-11	36.425063161978215	39.0	37.0	39.0	31.5	39.0
12-13	36.34010013043339	39.0	37.0	39.0	31.0	39.0
14-15	37.50083442027824	40.0	37.0	41.0	31.0	41.0
16-17	37.53658925728582	40.0	37.0	41.0	31.0	41.0
18-19	37.507886309261096	40.0	37.0	41.0	31.0	41.0
20-21	37.572246187063016	40.0	37.0	41.0	31.0	41.0
22-23	37.510609013793726	40.0	37.0	41.0	31.0	41.0
24-25	37.471159180513396	40.0	37.0	41.0	31.0	41.0
26-27	37.509069723098214	40.0	37.0	41.0	31.0	41.0
28-29	37.50403618190978	40.0	37.0	41.0	31.0	41.0
30-31	37.3674078233733	40.0	36.0	41.0	30.0	41.0
32-33	37.35943901725386	40.0	36.0	41.0	30.0	41.0
34-35	37.29528553570152	40.0	36.0	41.0	30.0	41.0
36-37	37.18092773316304	40.0	36.0	41.0	30.0	41.0
38-39	37.06692210899365	40.0	36.0	41.0	30.0	41.0
40-41	36.97235656642117	40.0	35.5	41.0	30.0	41.0
42-43	36.80957783388523	39.0	35.0	41.0	30.0	41.0
44-45	36.64379325691432	39.0	35.0	41.0	29.5	41.0
46-47	36.625882295167386	39.0	35.0	41.0	29.5	41.0
48-49	36.476473190473016	39.0	35.0	41.0	29.0	41.0
50-51	36.284973017465916	39.0	35.0	41.0	29.0	41.0
52-53	36.053714906473424	38.0	35.0	40.0	28.0	41.0
54-55	35.824761365252115	38.0	34.0	40.0	28.0	41.0
56-57	35.567941351000606	37.5	34.0	40.0	28.0	41.0
58-59	35.24393139548933	37.0	34.0	40.0	28.0	41.0
60-61	34.99056669485738	36.5	33.5	40.0	27.0	41.0
62-63	34.66980863605468	36.0	33.0	39.0	27.0	41.0
64-65	34.50107739047746	35.0	33.0	39.0	27.0	41.0
66-67	34.24415813342999	35.0	33.0	39.0	26.5	41.0
68-69	34.10719526708435	35.0	33.0	38.5	26.0	41.0
70-71	34.04044051473108	35.0	33.0	37.5	26.5	40.5
72-73	33.830079381908476	35.0	33.0	37.0	26.0	40.0
74-75	33.58319124970309	35.0	33.0	36.5	26.0	39.5
76-77	33.33734948175946	35.0	33.0	36.0	26.0	39.0
78-79	33.094616705007674	35.0	33.0	36.0	26.0	39.0
80-81	32.93031522224222	35.0	33.0	36.0	26.0	38.0
82-83	32.673482982428894	35.0	33.0	35.0	25.0	37.0
84-85	32.51605524474547	35.0	32.0	35.0	25.0	37.0
86-87	32.35319617280328	35.0	32.0	35.0	25.0	36.5
88-89	32.23403464909144	35.0	32.0	35.0	25.0	36.0
90-91	32.08521781636261	35.0	32.0	35.0	25.0	36.0
92-93	31.93182122653411	35.0	32.0	35.0	24.5	36.0
94-95	31.86880164015672	35.0	32.0	35.0	24.0	35.0
96-97	31.759802229449345	35.0	32.0	35.0	24.0	35.0
98-99	31.669048082826436	35.0	32.0	35.0	24.0	35.0
100	30.70306514354659	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.32292692039325743
1101	2	0.38729279615888856
1101	3	0.4134748809679465
1101	4	0.45939905558569194
1101	5	0.3784135878403063
1101	6	0.3948826708653854
1101	7	0.38947202795578306
1101	8	0.45432173952238486
1101	9	0.6064079918952672
1101	10-11	0.6390638367609043
1101	12-13	0.4736943554533042
1101	14-15	0.666898686646185
1101	16-17	0.6573794218143973
1101	18-19	0.6889779585463103
1101	20-21	0.6486091499869957
1101	22-23	0.5920911155497919
1101	24-25	0.6524343257952623
1101	26-27	0.5836655972991167
1101	28-29	0.5938762118072276
1101	30-31	0.6508252571994362
1101	32-33	0.5643132962313899
1101	34-35	0.5957829354687334
1101	36-37	0.6332058711382871
1101	38-39	0.5978199769316603
1101	40-41	0.5931629972542325
1101	42-43	0.5431992661505376
1101	44-45	0.5911886789636824
1101	46-47	0.5430237465035646
1101	48-49	0.5367348886532355
1101	50-51	0.4796973041569359
1101	52-53	0.430157759479755
1101	54-55	0.4650693801996013
1101	56-57	0.510535371077836
1101	58-59	0.5058932748440057
1101	60-61	0.44668813016413367
1101	62-63	0.43222575314769074
1101	64-65	0.41148112796334146
1101	66-67	0.42729409127174023
1101	68-69	0.4782409835435857
1101	70-71	0.45068103125049674
1101	72-73	0.47590046282346776
1101	74-75	0.42340018443866967
1101	76-77	0.43022293125183353
1101	78-79	0.44053879986233824
1101	80-81	0.42705905912772124
1101	82-83	0.4436209663633335
1101	84-85	0.4155258059076985
1101	86-87	0.4028158349089992
1101	88-89	0.47475966826250726
1101	90-91	0.38284224554733726
1101	92-93	0.39716316947457386
1101	94-95	0.3956092108671285
1101	96-97	0.4310508694338253
1101	98-99	0.40704232816053576
1101	100	0.5049870838254158
1102	1	0.2678649499488337
1102	2	0.34039513976856384
1102	3	0.33387276989800085
1102	4	0.4005679170920615
1102	5	0.30105648363983306
1102	6	0.36593188681769817
1102	7	0.3104318650061302
1102	8	0.3844021793571386
1102	9	0.4454903439151394
1102	10-11	0.5819893207060076
1102	12-13	0.5542951475557203
1102	14-15	0.732225204673135
1102	16-17	0.6097579968037365
1102	18-19	0.6979196740546882
1102	20-21	0.552244953932977
1102	22-23	0.6474877125052814
1102	24-25	0.6585029182272351
1102	26-27	0.6579095057744411
1102	28-29	0.6413199265609038
1102	30-31	0.6393176401992307
1102	32-33	0.6183400392023728
1102	34-35	0.6995705709465696
1102	36-37	0.6995882733227035
1102	38-39	0.6727653691336215
1102	40-41	0.5911303050013075
1102	42-43	0.5758678609836281
1102	44-45	0.6309258419821546
1102	46-47	0.5855586052954109
1102	48-49	0.5846308141193717
1102	50-51	0.5551709425008937
1102	52-53	0.5390480078692264
1102	54-55	0.47687960879317615
1102	56-57	0.5203670895279799
1102	58-59	0.5267694140709196
1102	60-61	0.4824689006392262
1102	62-63	0.4589456741712752
1102	64-65	0.4841526567125385
1102	66-67	0.5367102709990945
1102	68-69	0.49784062601229095
1102	70-71	0.5040624343026963
1102	72-73	0.49316611578380076
1102	74-75	0.48526047949851403
1102	76-77	0.48677422311261154
1102	78-79	0.46108313742386287
1102	80-81	0.4491510786326529
1102	82-83	0.4750527495965997
1102	84-85	0.4522040337784219
1102	86-87	0.41755072039563856
1102	88-89	0.43113738120309364
1102	90-91	0.462638133184079
1102	92-93	0.44415620364053865
1102	94-95	0.5124667795585438
1102	96-97	0.5298570661094644
1102	98-99	0.4714226798208685
1102	100	0.6022888540071136
1103	1	0.1904937170100638
1103	2	0.25322360553236933
1103	3	0.29848753617080703
1103	4	0.37509700732996976
1103	5	0.19641413217050285
1103	6	0.26450929259478073
1103	7	0.2725799826682689
1103	8	0.25959728534616744
1103	9	0.3894956527642819
1103	10-11	0.4376676013636924
1103	12-13	0.39650007414459765
1103	14-15	0.5106134272785425
1103	16-17	0.5039133471463373
1103	18-19	0.5522852386307449
1103	20-21	0.44365952897926064
1103	22-23	0.5374595104490183
1103	24-25	0.5533097206480235
1103	26-27	0.4078900168620052
1103	28-29	0.40079448736158696
1103	30-31	0.5082215286324399
1103	32-33	0.4462782144348765
1103	34-35	0.5049808260469888
1103	36-37	0.43608952198175643
1103	38-39	0.5387862768234584
1103	40-41	0.45998038206058567
1103	42-43	0.4405411614627539
1103	44-45	0.4283551368440044
1103	46-47	0.45178925493546274
1103	48-49	0.444720923833664
1103	50-51	0.371617926779642
1103	52-53	0.34507606727667195
1103	54-55	0.3369502970640639
1103	56-57	0.40307652063872723
1103	58-59	0.4037339340335606
1103	60-61	0.3583037287329489
1103	62-63	0.36614622973058175
1103	64-65	0.33149255510417674
1103	66-67	0.3434834005887879
1103	68-69	0.34234691171666043
1103	70-71	0.2983678839693482
1103	72-73	0.4158581302453115
1103	74-75	0.3585755222979756
1103	76-77	0.26821984355302675
1103	78-79	0.3514516340113758
1103	80-81	0.25128070014100956
1103	82-83	0.32019718634101224
1103	84-85	0.30888238865205153
1103	86-87	0.2970993911110895
1103	88-89	0.33748474263374817
1103	90-91	0.4156155320214978
1103	92-93	0.3107230225623212
1103	94-95	0.23687573636198067
1103	96-97	0.32236936234093605
1103	98-99	0.2804110587616577
1103	100	0.3734423335297805
1104	1	0.20785411952932975
1104	2	0.3154986838195484
1104	3	0.2614238625961036
1104	4	0.4301093808096752
1104	5	0.329214526245309
1104	6	0.30634643179936205
1104	7	0.31799757294776043
1104	8	0.39635766206291834
1104	9	0.4169218517016873
1104	10-11	0.5481354479090967
1104	12-13	0.46126368790437056
1104	14-15	0.586001110095232
1104	16-17	0.6480656758234886
1104	18-19	0.4962435792943438
1104	20-21	0.42263657646762454
1104	22-23	0.5184822283923225
1104	24-25	0.5607134010252111
1104	26-27	0.5370916635317258
1104	28-29	0.5119805821506489
1104	30-31	0.4751572451800641
1104	32-33	0.5343576861869792
1104	34-35	0.5358915376100342
1104	36-37	0.46574200899253526
1104	38-39	0.4441278669622051
1104	40-41	0.5633063264913503
1104	42-43	0.6285214108088937
1104	44-45	0.5633041768730962
1104	46-47	0.49444292651633504
1104	48-49	0.4900512546922968
1104	50-51	0.4420698226782136
1104	52-53	0.5160768530805981
1104	54-55	0.5476362921615916
1104	56-57	0.5481910486285884
1104	58-59	0.5083941031573289
1104	60-61	0.5734078184318747
1104	62-63	0.531247856461988
1104	64-65	0.454120893304804
1104	66-67	0.41688921553783587
1104	68-69	0.47468966296867166
1104	70-71	0.44490413064141876
1104	72-73	0.35813508507917646
1104	74-75	0.32976138251870424
1104	76-77	0.38269927777420776
1104	78-79	0.3347684302770517
1104	80-81	0.3619943743021423
1104	82-83	0.31250686345769196
1104	84-85	0.35465230057163666
1104	86-87	0.3820401039642576
1104	88-89	0.294030723891737
1104	90-91	0.42954349608562126
1104	92-93	0.40746064615051836
1104	94-95	0.46812305348663585
1104	96-97	0.4309923289266777
1104	98-99	0.3535128731477357
1104	100	0.4253555901592705
1105	1	0.1965562292058145
1105	2	0.2903334795801804
1105	3	0.3002757737605961
1105	4	0.3755054038971224
1105	5	0.2606018859801793
1105	6	0.2631386559121722
1105	7	0.335151233464444
1105	8	0.3094522249472931
1105	9	0.407435703056791
1105	10-11	0.41732516309720324
1105	12-13	0.3487610466383444
1105	14-15	0.44048928834455126
1105	16-17	0.407049956120197
1105	18-19	0.4190165749904651
1105	20-21	0.34883137400763076
1105	22-23	0.42482988572837144
1105	24-25	0.44485523979120956
1105	26-27	0.43965452566319385
1105	28-29	0.4985008670737514
1105	30-31	0.47306344409241774
1105	32-33	0.41229274440604513
1105	34-35	0.45012601530662977
1105	36-37	0.44078966299225186
1105	38-39	0.41655254917619544
1105	40-41	0.3859772581075802
1105	42-43	0.3900864026348714
1105	44-45	0.3342811394727647
1105	46-47	0.4359119708784789
1105	48-49	0.2995434365448588
1105	50-51	0.37108552013926044
1105	52-53	0.3844806109070831
1105	54-55	0.3915069424134856
1105	56-57	0.35546879304300916
1105	58-59	0.35912862533840695
1105	60-61	0.42114681130323817
1105	62-63	0.36510010126008297
1105	64-65	0.37591400710722667
1105	66-67	0.3125840353200786
1105	68-69	0.31168426366485136
1105	70-71	0.3770014904875012
1105	72-73	0.33565613179900566
1105	74-75	0.3088672722639174
1105	76-77	0.290666587241617
1105	78-79	0.3529893846915755
1105	80-81	0.2561223394707923
1105	82-83	0.3429174830152988
1105	84-85	0.29318706519313054
1105	86-87	0.27372189535429214
1105	88-89	0.30728556991982714
1105	90-91	0.2937165017907617
1105	92-93	0.31879415186121207
1105	94-95	0.2872384517895554
1105	96-97	0.22184045334977043
1105	98-99	0.2281801292337704
1105	100	0.3465413795919794
1106	1	-0.002558183753709642
1106	2	0.14335411780122342
1106	3	0.08691348659762
1106	4	0.2243098949391893
1106	5	0.08655650469854947
1106	6	0.1540727227434715
1106	7	0.1364095794958331
1106	8	0.1633186662936481
1106	9	0.20628395747051087
1106	10-11	0.18596357413182574
1106	12-13	0.12318892788199776
1106	14-15	0.17981404131650436
1106	16-17	0.06414073167615442
1106	18-19	0.20104457914642637
1106	20-21	0.15904775704306218
1106	22-23	0.15254242668908802
1106	24-25	0.17508831027451777
1106	26-27	0.22928472943266343
1106	28-29	0.2273311613944955
1106	30-31	0.2603884474044875
1106	32-33	0.14539981848102457
1106	34-35	0.18688390346977712
1106	36-37	0.20372321607962363
1106	38-39	0.2643596911539774
1106	40-41	0.31657277684907825
1106	42-43	0.20482359556530838
1106	44-45	0.1503686674230309
1106	46-47	0.1966040888850955
1106	48-49	0.10472182233814209
1106	50-51	0.09464488056640619
1106	52-53	0.06451701778659924
1106	54-55	0.14114288071820624
1106	56-57	0.11925493847116542
1106	58-59	0.13050623188240706
1106	60-61	0.15875134569688498
1106	62-63	0.11503105538252356
1106	64-65	0.184150299920816
1106	66-67	0.1927201345070202
1106	68-69	0.17692414963300962
1106	70-71	0.1707914066370506
1106	72-73	0.15271836922054405
1106	74-75	0.13112368550772402
1106	76-77	0.12092774288997532
1106	78-79	0.07537877248574176
1106	80-81	0.13490787289332218
1106	82-83	0.17487564078750495
1106	84-85	0.11277207745951756
1106	86-87	0.05438073218294193
1106	88-89	0.15816609655421132
1106	90-91	0.07252048057986116
1106	92-93	0.13648737808077982
1106	94-95	0.16245111841731585
1106	96-97	0.16283763592297618
1106	98-99	0.14403388275226803
1106	100	0.18779022817653512
1107	1	0.05540823102588632
1107	2	0.14501172106649918
1107	3	0.14650743886793194
1107	4	0.15417212680186765
1107	5	0.01852762103746386
1107	6	0.1308232318514797
1107	7	0.08800148167888722
1107	8	0.08858227295416299
1107	9	0.07803584007321263
1107	10-11	0.18433526550163748
1107	12-13	0.10673023839486007
1107	14-15	0.23993885812322446
1107	16-17	0.10444146774869978
1107	18-19	0.11036984216737267
1107	20-21	0.11511102042347687
1107	22-23	0.2291859085712602
1107	24-25	0.23882830686278567
1107	26-27	0.12768655069424995
1107	28-29	0.17003180824359987
1107	30-31	0.2662680305635732
1107	32-33	0.24348232042203932
1107	34-35	0.19129535839471146
1107	36-37	0.20252680619996966
1107	38-39	0.2782305145846635
1107	40-41	0.17008916933321672
1107	42-43	0.19923320703370706
1107	44-45	0.2949601759610587
1107	46-47	0.26727824950238954
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2216	90-91	-1.1494070411246256
2216	92-93	-1.110046389799205
2216	94-95	-1.135377853031951
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2216	100	-0.7902087650746417
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	1.0
2	6.0
3	29.0
4	44.0
5	50.0
6	41.0
7	175.0
8	480.0
9	839.0
10	1220.0
11	2047.0
12	3594.0
13	5572.0
14	8227.0
15	11075.0
16	14249.0
17	16655.0
18	19471.0
19	22489.0
20	25788.0
21	30026.0
22	34565.0
23	40238.0
24	46584.0
25	53435.0
26	59587.0
27	65424.0
28	75233.0
29	90318.0
30	105376.0
31	120944.0
32	149862.0
33	193348.0
34	278552.0
35	402773.0
36	648878.0
37	1117983.0
38	886941.0
39	334237.0
40	496.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.730512934014804	20.719260216748086	11.541955955041765	24.008270894195345
2	15.85822137652421	19.792354195863112	39.89616682352264	24.453257604090037
3	18.63009915402728	26.860522485605067	28.674568768443816	25.834809591923836
4	12.06119792683423	16.253436336787924	40.570494581797455	31.114871154580392
5	12.911999792876387	38.96975317152897	35.153007432819926	12.965239602774718
6	29.635040515894445	38.85005379379838	18.026593615592557	13.488312074714614
7	26.774611388317442	31.90320480840435	23.9568119746561	17.365371828622102
8	26.51244407230661	34.77995181614108	19.81412742524567	18.89347668630664
9	26.338148182621957	14.683554083272874	21.62428888356029	37.354008850544886
10-11	25.962420907167775	25.742596025317106	27.354056137379818	20.940926930135305
12-13	25.916084572732558	23.70420809969014	27.445259403919238	22.93444792365807
14-15	23.270355155172926	24.89564850489881	24.82381551331669	27.010180826611574
16-17	22.13202425277011	27.44552891818291	26.196840219564066	24.225606609482917
18-19	21.35395535910313	28.554448992406773	27.816362000221705	22.275233648268394
20-21	23.299139064683157	27.54587203090326	26.718860442179665	22.436128462233913
22-23	23.979989261909346	27.137904329899786	26.280336493049727	22.60176991514114
24-25	23.321872311816495	27.104964868494818	27.350917766973705	22.22224505271498
26-27	23.2125543685136	27.24592624632128	26.1892288827736	23.352290502391522
28-29	22.115716149712842	27.986407487696873	26.773119086024266	23.124757276566015
30-31	23.0413999421773	27.64249627203874	26.78971421737677	22.52638956840719
32-33	23.604200137037378	27.72874983137457	25.691960155391218	22.97508987619683
34-35	23.477176656631084	27.43936117487801	26.93916432626404	22.144297842226862
36-37	22.978890637257095	26.925695721258393	26.594858564546897	23.50055507693762
38-39	23.330979846453477	27.149043118537243	27.143536123626284	22.376440911383
40-41	22.790779581497926	26.074637725681065	27.899823016196084	23.234759676624925
42-43	22.753770717484123	26.438151972108543	27.061005832229363	23.747071478177972
44-45	22.930084157958014	27.567824737712836	26.953882672092234	22.548208432236915
46-47	22.93823302232466	27.240391196647145	26.442621638587312	23.378754142440883
48-49	23.776307348398493	26.82999386839649	26.614837987251565	22.778860795953452
50-51	23.204970054619427	26.473504362207322	26.828522942883108	23.49300264029014
52-53	22.57545235652679	27.33823255429671	26.44747974223547	23.638835346941033
54-55	22.502382910615882	27.21831612582097	26.49411270026622	23.78518826329693
56-57	23.916671067874017	26.438490952384964	26.19234872194076	23.45248925780026
58-59	22.554679850435168	27.038316097865927	27.286076905110757	23.120927146588148
60-61	22.611129549501598	27.796449074250084	26.36882443786599	23.223596938382325
62-63	23.09120816128668	26.997293524237094	25.86545627198864	24.04604204248759
64-65	22.124269515980743	27.38821370019978	26.417526355785327	24.069990428034146
66-67	23.051953122752334	26.735939004010557	26.374061990614354	23.83804588262275
68-69	22.300842368000435	26.843797957846565	26.784947576618766	24.070412097534234
70-71	22.366620989726847	26.518674340611746	26.83284757276057	24.281857096900836
72-73	23.05058491930601	26.56263854453784	27.443200014466424	22.943576521689725
74-75	23.298050148434328	26.912053290661568	26.811237341109823	22.978659219794284
76-77	22.96377223104536	26.916059678501682	26.41149857227506	23.708669518177896
78-79	22.85164259153105	26.890525852664837	26.343195701584698	23.91463585421942
80-81	22.350330935713046	27.330138195893312	27.1838684945379	23.13566237385574
82-83	23.31142726571837	26.098687033240893	28.419670582936202	22.17021511810454
84-85	23.51864750407469	26.80682934738282	26.793924322509312	22.880598826033175
86-87	22.052585657029944	27.592993072474346	27.295842251659547	23.058579018836163
88-89	23.417126968795284	26.11535141718533	26.96680204571722	23.500719568302173
90-91	22.92358664028741	26.25958975154625	27.11632645407425	23.700497154092083
92-93	23.405561711123752	26.53768194087805	27.056051106193962	23.000705241804233
94-95	22.0699514320063	26.63519488895097	27.800134244971698	23.494719434071037
96-97	22.476964160970017	26.57945544582431	27.238856691245832	23.704723701959843
98-99	21.74904626525385	27.433007390481045	26.960624856798514	23.857321487466596
100	22.79723673853072	26.565314941805095	25.91897726446344	24.718471055200748
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	437.0
1	306.0
2	227.0
3	292.0
4	264.5
5	219.0
6	225.0
7	237.5
8	245.0
9	245.5
10	246.0
11	242.5
12	242.5
13	264.0
14	291.0
15	465.0
16	595.5
17	679.0
18	904.0
19	1110.0
20	1439.0
21	1860.5
22	2527.0
23	3701.0
24	5010.0
25	6741.0
26	9298.5
27	11657.5
28	15955.5
29	25895.5
30	40051.5
31	53684.5
32	71752.0
33	88165.0
34	97993.5
35	111422.0
36	123486.5
37	144871.5
38	153432.5
39	142429.0
40	130993.0
41	117062.5
42	114581.0
43	124937.0
44	140965.5
45	173899.0
46	205631.5
47	226888.0
48	240165.0
49	232484.5
50	218421.0
51	227959.0
52	236332.5
53	225689.5
54	213242.0
55	181832.0
56	157796.5
57	128453.5
58	97860.5
59	96787.0
60	81426.5
61	53882.5
62	38914.5
63	32988.0
64	24065.0
65	10593.5
66	5054.0
67	3148.5
68	2059.0
69	1325.0
70	842.0
71	541.5
72	320.5
73	184.0
74	115.0
75	77.5
76	59.0
77	49.5
78	43.0
79	35.0
80	30.5
81	31.0
82	24.5
83	16.0
84	14.0
85	14.0
86	12.0
87	11.0
88	9.0
89	9.5
90	10.0
91	8.0
92	8.0
93	12.0
94	9.5
95	5.0
96	4.0
97	4.0
98	5.0
99	5.0
100	7.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06264829914696399
2	0.008054487787999305
3	0.007869563323478913
4	0.009821543782305276
5	0.003965602405826189
6	0.0024656595269385633
7	8.21886508979521E-4
8	0.001582131529785578
9	6.780563699081049E-4
10-11	4.828583240254686E-4
12-13	2.362923713316123E-4
14-15	1.541037204336602E-4
16-17	5.444998121989326E-4
18-19	0.0016745937620457742
20-21	0.0010684524616733774
22-23	0.0013355655770917216
24-25	0.0013663863211784537
26-27	0.003688215709045601
28-29	0.004150526870346581
30-31	0.005157337843846495
32-33	0.0060922337478107
34-35	0.0034519233377139887
36-37	0.008414063135677847
38-39	0.00645180909548924
40-41	0.006266884630968849
42-43	0.014434381813952839
44-45	0.020588257049937
46-47	0.018708191660646346
48-49	0.022139567835635848
50-51	0.01868764449792186
52-53	0.0256531326615233
54-55	0.019601993239161578
56-57	0.015677485158784362
58-59	0.01784521082621785
60-61	0.016376088691416955
62-63	0.01151668470707554
64-65	0.011465316800264318
66-67	0.014300825256243667
68-69	0.005619649005147475
70-71	0.00880445922744312
72-73	0.008455157461126824
74-75	0.011475590381626563
76-77	0.011167382940759243
78-79	0.012194741076983644
80-81	0.010253034199519525
82-83	0.006492903420938217
84-85	0.010900269825340897
86-87	0.009616072155060397
88-89	0.00806476136936155
90-91	0.013448118003177415
92-93	0.008958562947876779
94-95	0.0077154596030452535
96-97	0.009605798573698152
98-99	0.009390053365091027
100	0.261627022970906
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4866852.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.43878998082504
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.76835473676996	49.67519017568085
2	14.994428176042145	19.92423328941259
3	5.227102421130303	10.418470798972148
4	2.211548201731405	5.877303460292164
5	1.1125758084734012	3.695909523845546
6	0.5963795891507928	2.377364296346415
7	0.3471782368705352	1.6146271375748227
8	0.21666779871677092	1.1516117099640972
9	0.13343483384629706	0.7978724011836373
>10	0.38579991244532874	3.623184365736725
>50	0.003987516695334493	0.1765227881003494
>100	0.002274078282990827	0.27096206197800654
>500	8.95632815880905E-5	0.042783922838306884
>1k	1.3434492235081706E-4	0.12802449230261584
>5k	4.4781640783605685E-5	0.22593957577180215
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	7594	0.15603515372976207	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0547162724488027E-5
2	0.0	0.0	0.0	0.0	2.0547162724488027E-5
3	0.0	0.0	0.0	0.0	4.1094325448976054E-5
4	0.0	0.0	0.0	0.0	4.1094325448976054E-5
5	0.0	0.0	0.0	0.0	8.218865089795211E-5
6	0.0	0.0	0.0	0.0	8.218865089795211E-5
7	0.0	0.0	0.0	0.0	8.218865089795211E-5
8	0.0	0.0	0.0	0.0	8.218865089795211E-5
9	0.0	0.0	0.0	0.0	8.218865089795211E-5
10-11	0.0	0.0	0.0	0.0	8.218865089795211E-5
12-13	0.0	0.0	0.0	0.0	1.1300939498468415E-4
14-15	0.0	0.0	0.0	0.0	1.541037204336602E-4
16-17	0.0	0.0	0.0	0.0	1.8492446452039222E-4
18-19	0.0	0.0	0.0	1.0273581362244013E-5	2.260187899693683E-4
20-21	0.0	0.0	0.0	5.1367906811220065E-5	2.6711311541834434E-4
22-23	0.0	0.0	0.0	1.6437730179590421E-4	3.082074408673204E-4
24-25	0.0	0.0	0.0	3.390281849540524E-4	3.082074408673204E-4
26-27	0.0	0.0	0.0	7.91065764892789E-4	3.390281849540524E-4
28-29	0.0	0.0	0.0	0.0032669988731935965	3.6984892904078445E-4
30-31	0.0	0.0	0.0	0.012389939122866279	3.801225104030285E-4
32-33	0.0	0.0	0.0	0.028550282605676113	3.903960917652725E-4
34-35	0.0	0.0	0.0	0.053340434432770915	3.903960917652725E-4
36-37	0.0	0.0	0.0	0.09625318378286415	3.903960917652725E-4
38-39	0.0	0.0	0.0	0.16158288766537385	4.2121683585200455E-4
40-41	0.0	0.0	0.0	0.23336440064337277	4.3149041721424853E-4
42-43	0.0	0.0	0.0	0.30952245928168765	4.4176399857649256E-4
44-45	0.0	0.0	0.0	0.4016662105196542	4.520375799387366E-4
46-47	0.0	0.0	0.0	0.5197610282786491	4.623111613009806E-4
48-49	0.0	0.0	0.0	0.6552284721211987	4.725847426632246E-4
50-51	0.0	0.0	0.0	0.7986682151008495	4.931319053877127E-4
52-53	0.0	0.0	0.0	0.9410395056188271	5.547733935611768E-4
54-55	0.0	0.0	0.0	1.095800735259671	5.650469749234207E-4
56-57	0.0	0.0	0.0	1.288718046079889	5.855941376479087E-4
58-59	0.0	0.0	0.0	1.5211372772379352	5.958677190101528E-4
60-61	0.0	0.0	0.0	1.765309485474389	5.958677190101528E-4
62-63	0.0	0.0	0.0	2.026104348354953	6.369620444591288E-4
64-65	0.0	0.0	0.0	2.2963611796701437	6.369620444591288E-4
66-67	0.0	0.0	0.0	2.602822111705883	6.369620444591288E-4
68-69	0.0	0.0	0.0	2.94919590733394	6.369620444591288E-4
70-71	0.0	0.0	0.0	3.330766992709045	6.369620444591288E-4
72-73	0.0	0.0	0.0	3.7324640239727858	6.677827885458608E-4
74-75	0.0	0.0	0.0	4.1166035046884515	6.986035326325929E-4
76-77	0.0	0.0	0.0	4.55981607823702	6.986035326325929E-4
78-79	0.0	0.0	0.0	5.03393158452322	6.986035326325929E-4
80-81	0.0	0.0	0.0	5.550661906300007	7.088771139948369E-4
82-83	0.0	0.0	0.0	6.0934152096673575	7.396978580815689E-4
84-85	0.0	0.0	0.0	6.6213540087103535	7.60245020806057E-4
86-87	0.0	0.0	0.0	7.182229909600703	8.01339346255033E-4
88	0.0	0.0	0.0	7.639948780032761	8.21886508979521E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	5625	0.0	45.573837	94
GTACTAG	5915	0.0	36.727207	1
CTGTGCG	8260	0.0	33.62295	9
TAGGCAT	7280	0.0	33.243652	5
TAGTACT	8440	0.0	33.18597	4
GCATCGT	6215	0.0	32.36168	8
ACTGTGC	8850	0.0	31.912409	8
TACTGTG	9755	0.0	31.312265	7
GTACTGT	9035	0.0	31.206963	6
CAGTACG	575	0.0	31.055902	9
CTAGGCA	8190	0.0	30.5266	4
TCTAACG	3105	0.0	29.817327	2
TATTCTC	2730	0.0	29.779425	5
CTAGTAC	9770	0.0	29.726864	3
TACCCCG	260	0.0	28.918749	5
ACGTTAA	755	0.0	28.008766	8
CTAACGC	3285	0.0	27.896803	3
TAACGCC	3320	0.0	27.885527	4
CGCCTAC	3290	0.0	27.852688	7
CAACGCA	10520	0.0	27.338116	5
>>END_MODULE