FastQCFastQC Report
Mon 16 Jan 2017
ERR1146639_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146639_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4866852
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123020.2527711958366517No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102110.20980707857974723No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65150.1338647651500395No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC54550.045.7802694
GTACTAG59000.035.8824271
GTATCAA196200.034.8647651
GGTATCA123550.034.841731
CTGTGCG80700.032.9670649
TAGTACT82500.032.50144
TAGGCAT76450.031.7854335
ACTGTGC86850.031.6566858
GTACTGT88500.030.3223826
TACTGTG98750.029.8880427
CAGTACG6850.029.5063369
CAACGCA223500.029.3787965
GAACCGT5150.029.2020446
TATTCGC3900.028.925719
ATCAACG227250.028.918363
CTAGTAC96350.028.6594283
CTAGGCA86800.028.5067144
TCTAACG26150.028.2455042
TATTCTC28800.028.0705575
CCGTGTT5000.027.2624849
Produced by FastQC (version 0.11.5)