##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146639_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4866852
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14518275879357	33.0	31.0	34.0	30.0	34.0
2	32.22347936612825	33.0	31.0	34.0	30.0	34.0
3	32.36692671155811	34.0	31.0	34.0	30.0	34.0
4	35.967915194462456	37.0	35.0	37.0	35.0	37.0
5	35.88049544140648	37.0	35.0	37.0	35.0	37.0
6	35.91841502474289	37.0	35.0	37.0	35.0	37.0
7	35.84223806271487	37.0	35.0	37.0	35.0	37.0
8	35.852905327714915	37.0	35.0	37.0	35.0	37.0
9	37.56248618203307	39.0	37.0	39.0	35.0	39.0
10-11	37.49776118114954	39.0	37.0	39.0	34.5	39.0
12-13	37.45707523055971	39.0	37.0	39.0	34.0	39.0
14-15	38.676696353207376	40.0	38.0	41.0	34.0	41.0
16-17	38.68445044147634	40.0	38.0	41.0	34.0	41.0
18-19	38.74631106514026	40.0	38.0	41.0	34.0	41.0
20-21	38.74246967033311	40.0	38.0	41.0	34.0	41.0
22-23	38.69892961610503	40.0	38.0	41.0	34.0	41.0
24-25	38.617778905132106	40.0	38.0	41.0	34.0	41.0
26-27	38.47313047530518	40.0	38.0	41.0	34.0	41.0
28-29	38.41709445859459	40.0	38.0	41.0	34.0	41.0
30-31	38.27730224794179	40.0	37.5	41.0	34.0	41.0
32-33	38.126261082112215	40.0	37.0	41.0	33.5	41.0
34-35	38.01994513085666	40.0	37.0	41.0	33.0	41.0
36-37	38.003212446156155	40.0	37.0	41.0	33.0	41.0
38-39	37.8959484488125	40.0	37.0	41.0	33.0	41.0
40-41	37.77233959446475	40.0	37.0	41.0	33.0	41.0
42-43	37.622880765636594	40.0	36.5	41.0	32.5	41.0
44-45	37.38984059922102	40.0	36.0	41.0	32.0	41.0
46-47	37.12084135700037	40.0	35.5	41.0	31.0	41.0
48-49	36.83181469253637	39.0	35.0	41.0	31.0	41.0
50-51	36.59469920186601	39.0	35.0	41.0	30.0	41.0
52-53	36.39102031456884	39.0	35.0	41.0	30.0	41.0
54-55	36.19405315797563	38.5	35.0	41.0	30.0	41.0
56-57	35.946501249678434	38.0	35.0	40.0	29.5	41.0
58-59	35.64548172001121	37.0	35.0	40.0	29.0	41.0
60-61	35.45512078444136	37.0	35.0	40.0	29.0	41.0
62-63	35.22740788090536	36.0	34.0	40.0	29.0	41.0
64-65	34.97570133630528	36.0	34.0	39.0	29.0	41.0
66-67	34.728376576892	35.0	34.0	39.0	29.0	41.0
68-69	34.478830258244955	35.0	34.0	39.0	29.0	41.0
70-71	34.274964391767	35.0	34.0	37.5	28.5	40.5
72-73	34.05455826476745	35.0	34.0	37.0	28.5	40.0
74-75	33.81457480112401	35.0	34.0	37.0	28.0	39.5
76-77	33.58750800311988	35.0	33.5	36.0	28.0	39.0
78-79	33.379265796453225	35.0	33.0	36.0	27.0	39.0
80-81	33.27178728673073	35.0	33.0	36.0	28.0	38.0
82-83	33.120060153873595	35.0	33.0	35.0	27.0	37.0
84-85	33.01013653178687	35.0	33.0	35.0	28.0	37.0
86-87	32.88229814672811	35.0	33.0	35.0	27.0	36.5
88-89	32.78749456527546	35.0	33.0	35.0	27.0	36.0
90-91	32.731047810782	35.0	33.0	35.0	27.0	36.0
92-93	32.661972050927375	35.0	33.0	35.0	27.0	36.0
94-95	32.62550864501324	35.0	33.0	35.0	27.0	35.5
96-97	32.53867623260375	35.0	33.0	35.0	27.0	35.0
98-99	32.473431080295846	35.0	33.0	35.0	27.0	35.0
100	31.245703177331055	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.33863881972774124
1101	2	0.4300479514727229
1101	3	0.33772922493876933
1101	4	0.21873364742825885
1101	5	0.21195816603582074
1101	6	0.21590843192658582
1101	7	0.27717834211694026
1101	8	0.27578128149293946
1101	9	0.31715156573137904
1101	10-11	0.3856169054325349
1101	12-13	0.38816185204613873
1101	14-15	0.489244706939175
1101	16-17	0.5001610890549912
1101	18-19	0.4885389566296183
1101	20-21	0.503796275112812
1101	22-23	0.4580421316223777
1101	24-25	0.44207307200589696
1101	26-27	0.4888905554671581
1101	28-29	0.5193907441138634
1101	30-31	0.5685550594469291
1101	32-33	0.5857992120042468
1101	34-35	0.6501393701502138
1101	36-37	0.5499046870269453
1101	38-39	0.5602000834574312
1101	40-41	0.5524259493733794
1101	42-43	0.5120857782484336
1101	44-45	0.5191255155105807
1101	46-47	0.550257413653199
1101	48-49	0.5566218966583207
1101	50-51	0.5469164620343818
1101	52-53	0.5719919352114147
1101	54-55	0.540182139502619
1101	56-57	0.5363754481825751
1101	58-59	0.48468677530116366
1101	60-61	0.48671629558904783
1101	62-63	0.4348537388066305
1101	64-65	0.48135333047624584
1101	66-67	0.43368786855908326
1101	68-69	0.4580177530877947
1101	70-71	0.44952397637955244
1101	72-73	0.49930388870910747
1101	74-75	0.49625439996932386
1101	76-77	0.47241550727365933
1101	78-79	0.48151241448201176
1101	80-81	0.4979306421587779
1101	82-83	0.46132548724733624
1101	84-85	0.4044101853931892
1101	86-87	0.37206034320544035
1101	88-89	0.43733023041157537
1101	90-91	0.4187109989667377
1101	92-93	0.40326150649823234
1101	94-95	0.4382200940055512
1101	96-97	0.45000338965666487
1101	98-99	0.3737606782313776
1101	100	0.789156281129543
1102	1	0.3689884908960863
1102	2	0.41802525132671775
1102	3	0.34502788072810375
1102	4	0.2643055095798701
1102	5	0.24396672782919637
1102	6	0.21663304407723416
1102	7	0.2837289238429008
1102	8	0.3132483867535214
1102	9	0.3551116850308844
1102	10-11	0.34194106400713054
1102	12-13	0.34039739960598325
1102	14-15	0.4818302717109617
1102	16-17	0.45681018335061907
1102	18-19	0.44272323575136596
1102	20-21	0.46221419361114613
1102	22-23	0.5140541525962306
1102	24-25	0.5031594574345917
1102	26-27	0.5303453815943726
1102	28-29	0.5339581201751358
1102	30-31	0.5642845009066733
1102	32-33	0.5673463980302955
1102	34-35	0.5843077736392388
1102	36-37	0.6495987876270704
1102	38-39	0.573859644355565
1102	40-41	0.5345294730623422
1102	42-43	0.5306905453795281
1102	44-45	0.5924359301908595
1102	46-47	0.5984984803337596
1102	48-49	0.5893721177613429
1102	50-51	0.5080993747939644
1102	52-53	0.5077300505167557
1102	54-55	0.5212608719535581
1102	56-57	0.5072295928471533
1102	58-59	0.4884118639875936
1102	60-61	0.4293291632790073
1102	62-63	0.5178513536167912
1102	64-65	0.3815652252893429
1102	66-67	0.4464858907590781
1102	68-69	0.4146839141594043
1102	70-71	0.4075070844301365
1102	72-73	0.44099177637504283
1102	74-75	0.44703150324544794
1102	76-77	0.4317901853099073
1102	78-79	0.4117188542042385
1102	80-81	0.35275756582572626
1102	82-83	0.3685389974798312
1102	84-85	0.3292126560973827
1102	86-87	0.3940775435338395
1102	88-89	0.386263768776395
1102	90-91	0.3870952672756047
1102	92-93	0.3849313575245006
1102	94-95	0.38734605536527766
1102	96-97	0.34197622970707897
1102	98-99	0.3757645531349709
1102	100	0.6231395905288473
1103	1	0.28876366479752136
1103	2	0.3826563836595156
1103	3	0.27362952716530486
1103	4	0.20401672283696115
1103	5	0.2197809875059491
1103	6	0.19452120547858698
1103	7	0.22246763844214001
1103	8	0.18966566538038876
1103	9	0.26915361028869
1103	10-11	0.33141620311381814
1103	12-13	0.311493636472413
1103	14-15	0.4250216244940219
1103	16-17	0.3754963974028769
1103	18-19	0.40441726753699214
1103	20-21	0.4284632237437833
1103	22-23	0.3742137265003649
1103	24-25	0.4134136036476974
1103	26-27	0.44161322112987733
1103	28-29	0.46217395939834915
1103	30-31	0.42655258814991015
1103	32-33	0.39545581930983786
1103	34-35	0.412760718325238
1103	36-37	0.4203080413906406
1103	38-39	0.43898777287058266
1103	40-41	0.4864836654435507
1103	42-43	0.39331575585882206
1103	44-45	0.38023189033786764
1103	46-47	0.4838825863642384
1103	48-49	0.4810805204512363
1103	50-51	0.4633859591548415
1103	52-53	0.41579391092139417
1103	54-55	0.44471497807078464
1103	56-57	0.3828532525025068
1103	58-59	0.3678291821320414
1103	60-61	0.41194966056434623
1103	62-63	0.41338473006604914
1103	64-65	0.3771834103145011
1103	66-67	0.44964147531560883
1103	68-69	0.43138960363027934
1103	70-71	0.48835189312168836
1103	72-73	0.4525058778850948
1103	74-75	0.42082161479104485
1103	76-77	0.4261932915842337
1103	78-79	0.355530073919077
1103	80-81	0.38558180995567426
1103	82-83	0.3850226367209757
1103	84-85	0.4316329823969056
1103	86-87	0.40668717830468637
1103	88-89	0.41759843157097976
1103	90-91	0.38698936980733123
1103	92-93	0.38489195560857326
1103	94-95	0.33902781742512644
1103	96-97	0.38420856800067327
1103	98-99	0.3155221079800725
1103	100	0.4993583945341946
1104	1	0.22224102308892668
1104	2	0.25918664883901954
1104	3	0.19135280689977208
1104	4	0.1990180716048897
1104	5	0.18926505445175223
1104	6	0.21347432699671032
1104	7	0.2093360338774417
1104	8	0.2284707742787404
1104	9	0.20687524804480972
1104	10-11	0.25093275777410184
1104	12-13	0.2804590728281582
1104	14-15	0.2865503063372543
1104	16-17	0.27187794625759665
1104	18-19	0.3312732308505062
1104	20-21	0.31959114558561197
1104	22-23	0.3409889983064218
1104	24-25	0.3424555092808603
1104	26-27	0.34963780389144716
1104	28-29	0.3858336945706995
1104	30-31	0.34802098593089426
1104	32-33	0.38811711436162
1104	34-35	0.486960547090348
1104	36-37	0.3635359362571151
1104	38-39	0.4344265915844474
1104	40-41	0.4424066332021326
1104	42-43	0.4999730397920672
1104	44-45	0.4156918202019284
1104	46-47	0.45536165700448805
1104	48-49	0.4780205765130674
1104	50-51	0.4962848719306052
1104	52-53	0.4874184139297313
1104	54-55	0.4713049408917058
1104	56-57	0.4981998858604726
1104	58-59	0.44871350475732896
1104	60-61	0.4708305350350983
1104	62-63	0.4856716540541868
1104	64-65	0.4739707623898468
1104	66-67	0.3827559563918115
1104	68-69	0.4125070871811758
1104	70-71	0.4169406127304285
1104	72-73	0.3696361688671175
1104	74-75	0.413368909631707
1104	76-77	0.3756452226680622
1104	78-79	0.3780498069803073
1104	80-81	0.32514762396644414
1104	82-83	0.3144215553609442
1104	84-85	0.310184288356119
1104	86-87	0.3204413272321176
1104	88-89	0.3667024978130726
1104	90-91	0.3633371408217201
1104	92-93	0.3077841831867758
1104	94-95	0.28294675060832475
1104	96-97	0.29777412398626524
1104	98-99	0.26773420466913933
1104	100	0.4754108440140392
1105	1	0.16361170114361556
1105	2	0.18578758968578057
1105	3	0.1652105566523332
1105	4	0.2087746302854825
1105	5	0.17877589198330668
1105	6	0.19429078403026523
1105	7	0.18075903680229288
1105	8	0.17966040049789456
1105	9	0.23027775474528767
1105	10-11	0.23602664783071248
1105	12-13	0.2820334341978423
1105	14-15	0.33660057380019737
1105	16-17	0.30966712318349465
1105	18-19	0.29905907144036803
1105	20-21	0.3504832818415764
1105	22-23	0.32475202391836433
1105	24-25	0.2608853739368229
1105	26-27	0.30071388347418093
1105	28-29	0.3314813051980465
1105	30-31	0.3268435373382701
1105	32-33	0.34617967622285306
1105	34-35	0.3117503579201042
1105	36-37	0.31769087014205866
1105	38-39	0.30528797794328
1105	40-41	0.3423779407122183
1105	42-43	0.3184080201632895
1105	44-45	0.33123820083338984
1105	46-47	0.4000855751975365
1105	48-49	0.42443882515907205
1105	50-51	0.39488703054001917
1105	52-53	0.4069961449988
1105	54-55	0.39246923875978723
1105	56-57	0.3884887569555673
1105	58-59	0.358847403043562
1105	60-61	0.35838544556568763
1105	62-63	0.3951329747984076
1105	64-65	0.3425416984545251
1105	66-67	0.3351549210155227
1105	68-69	0.3572315614302184
1105	70-71	0.4158803529410591
1105	72-73	0.3127346241435305
1105	74-75	0.36475871601300724
1105	76-77	0.2661611592331852
1105	78-79	0.2213010386443628
1105	80-81	0.24039826621113036
1105	82-83	0.26998806521982033
1105	84-85	0.27300588334716736
1105	86-87	0.301386233203047
1105	88-89	0.3113494578548952
1105	90-91	0.2963471553903858
1105	92-93	0.3099142669288071
1105	94-95	0.3374503316090127
1105	96-97	0.2948106791081031
1105	98-99	0.3420169480270445
1105	100	0.5370117848608018
1106	1	0.11106984727209834
1106	2	0.20912449281523493
1106	3	0.11811333409286817
1106	4	0.13122833546831458
1106	5	0.094576112759718
1106	6	0.1240975201815786
1106	7	0.14666618816605137
1106	8	0.13312347902247268
1106	9	0.13290620528195518
1106	10-11	0.17057808711599165
1106	12-13	0.11211807911885074
1106	14-15	0.17478085673990762
1106	16-17	0.1584375350263585
1106	18-19	0.16177522408620604
1106	20-21	0.16418539896922368
1106	22-23	0.22325437978500418
1106	24-25	0.17675407260414744
1106	26-27	0.23241210392939848
1106	28-29	0.2068418968498591
1106	30-31	0.23949015421806763
1106	32-33	0.2327253450477258
1106	34-35	0.24752017295098483
1106	36-37	0.19092971215074783
1106	38-39	0.21242377531631007
1106	40-41	0.21423312285914875
1106	42-43	0.23667383550316146
1106	44-45	0.14293793426305257
1106	46-47	0.10436492154146038
1106	48-49	0.12168343493022604
1106	50-51	0.14742079237668548
1106	52-53	0.13200934713857748
1106	54-55	0.0565743477429379
1106	56-57	-0.017604607412557982
1106	58-59	-0.056324819739550946
1106	60-61	-0.043665646537753844
1106	62-63	0.06023120844511709
1106	64-65	0.047535359013487266
1106	66-67	0.06075129457693862
1106	68-69	0.06901688689864471
1106	70-71	0.08765857112148723
1106	72-73	0.05601931793979986
1106	74-75	0.07620772238426099
1106	76-77	0.10496522464026015
1106	78-79	0.12556022323703786
1106	80-81	0.11509653217627402
1106	82-83	0.1157358650880127
1106	84-85	0.06705663849251664
1106	86-87	-0.00484819065046338
1106	88-89	0.056060650131293244
1106	90-91	0.05264957967101935
1106	92-93	0.05738862552633606
1106	94-95	0.04527821894431128
1106	96-97	-0.010617079450419453
1106	98-99	-0.021222125081216348
1106	100	0.11637409275529009
1107	1	0.1336163535667083
1107	2	0.17600727608179056
1107	3	0.1427964858843751
1107	4	0.11096765983092638
1107	5	0.0370425065019333
1107	6	0.022866247866623723
1107	7	0.05108871977289198
1107	8	0.09191621219234491
1107	9	0.06914926095448237
1107	10-11	0.1646116337967598
1107	12-13	0.08520590105571557
1107	14-15	0.14968372435976107
1107	16-17	0.14663612066301113
1107	18-19	0.10818318888592415
1107	20-21	0.1715464883324742
1107	22-23	0.13787192095154666
1107	24-25	0.10155777688779466
1107	26-27	0.19228814074806877
1107	28-29	0.14623594100533666
1107	30-31	0.13898022655129694
1107	32-33	0.19130479317249893
1107	34-35	0.1623149115686715
1107	36-37	0.07648925406410001
1107	38-39	0.12818467370037467
1107	40-41	0.16295673282048284
1107	42-43	0.19235866869061624
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2216	66-67	-0.8705415207808684
2216	68-69	-0.8365562275160627
2216	70-71	-0.8523811293024508
2216	72-73	-0.7975698068225086
2216	74-75	-0.6772184183257295
2216	76-77	-0.7911156612444543
2216	78-79	-0.7360751433950767
2216	80-81	-0.807795514433785
2216	82-83	-0.8183797261554275
2216	84-85	-0.8003266998629357
2216	86-87	-0.7665061705489933
2216	88-89	-0.6931113940469729
2216	90-91	-0.730757743985933
2216	92-93	-0.7915145987657937
2216	94-95	-0.8119520404473519
2216	96-97	-0.8316499775235826
2216	98-99	-0.8127703860826045
2216	100	-0.2702538730687145
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	2.0
12	11.0
13	73.0
14	258.0
15	880.0
16	2063.0
17	4186.0
18	7495.0
19	11962.0
20	17090.0
21	24700.0
22	34158.0
23	40410.0
24	40449.0
25	38094.0
26	39376.0
27	46802.0
28	58811.0
29	73580.0
30	92358.0
31	115377.0
32	145687.0
33	187776.0
34	268280.0
35	396030.0
36	643513.0
37	1209469.0
38	985783.0
39	381454.0
40	722.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.23588553750967	21.864913911497617	12.844360173681057	25.05484037731166
2	14.49873305716973	20.72535996033239	39.51166345658499	25.26424352591289
3	17.40611795879554	27.211860972965685	29.376710037617748	26.005311030621026
4	11.614712351502178	16.959101851023508	39.27982732966645	32.146358467807865
5	12.338435426568282	39.04420512757288	35.19168295103593	13.425676494822905
6	29.568571046981106	39.22407717517491	17.393118752375766	13.81423302546822
7	25.911410496970117	32.61660720317774	23.979586804776474	17.492395495075666
8	26.891592347579092	34.20210846765014	19.417623548034747	19.488675636736026
9	26.41317221070211	14.005007754499212	21.67631150484954	37.90550852994913
10-11	25.893092701401233	25.837985210974157	27.081684423524692	21.187237664099914
12-13	26.076140196160217	23.632500198280688	27.93048587193574	22.36087373362335
14-15	23.271290135814855	24.555153304800758	25.745846362815794	26.427710196568587
16-17	21.441375246257746	26.992006331813666	27.231925277366148	24.33469314456244
18-19	20.81809803241296	27.215070036581164	30.01862909465353	21.948202836352344
20-21	22.519515695155718	26.375159959661808	29.199408570468137	21.905915774714334
22-23	23.056413057146592	25.911461864876927	28.887574555379945	22.144550522596536
24-25	22.471568890938126	25.941070326362915	29.85740063597578	21.72996014672318
26-27	22.41953320133836	26.13183018509706	28.669784904081734	22.778851709482844
28-29	21.440460897516505	26.84864877748491	29.285912125538232	22.424978199460348
30-31	22.43033073535008	26.440243097591626	29.18151199173511	21.947914175323184
32-33	23.11574298951355	26.599596618101394	28.21290846732138	22.071751925063676
34-35	22.792916242367756	26.433894024309758	29.201031796323374	21.572157936999112
36-37	22.45597359443024	25.81336971003022	28.8322410461629	22.89841564937664
38-39	22.655291346439135	25.941943580778705	29.583681607741514	21.819083465040645
40-41	22.13293110207584	25.118875609942528	30.036469159119694	22.711724128861942
42-43	22.391763710916216	25.374143286050206	29.02723362041829	23.206859382615292
44-45	22.58419816341241	26.441496474517816	28.704941099503333	22.26936426256644
46-47	22.66026375981846	26.286776339202422	27.90121828237226	23.151741618606852
48-49	23.721956204955482	26.17518469844573	27.697965748701627	22.40489334789716
50-51	22.88087864599129	26.014567527428405	27.633447657746736	23.47110616883357
52-53	22.15014962443896	26.82184500371082	27.234267653916746	23.79373771793348
54-55	22.21689708254946	26.835529414085325	27.072582030437747	23.87499147292747
56-57	23.637877215086878	26.01201967925057	26.805962046924787	23.544141058737765
58-59	22.196557671833627	26.588616891228344	27.924716831816216	23.290108605121812
60-61	22.415803891303867	27.32761341417409	26.9379570202669	23.318625674255145
62-63	22.969642388961077	26.387200597018364	26.423733452342503	24.219423561678063
64-65	21.933311902636007	26.77846792294113	26.95364281273896	24.334577361683905
66-67	22.75232815003225	26.169859048373617	26.977003797325043	24.100809004269088
68-69	22.17992246322674	26.003379597324923	27.506312088388963	24.310385851059372
70-71	22.20400593487053	25.735668954056123	27.43594443116863	24.624380679904718
72-73	22.855577157552528	25.634848034677166	28.01037594460793	23.499198863162377
74-75	23.01059107202114	26.245208641755884	27.31556545475653	23.428634831466447
76-77	22.682021300028275	26.253306041199558	26.76484862481836	24.299824033953804
78-79	22.405306592979056	26.362063851753025	26.92870329000176	24.303926265266156
80-81	21.932616812674805	26.905430861674034	27.623533651732167	23.538418673918994
82-83	22.89553904659521	25.610117176359587	28.878605718850707	22.615738058194495
84-85	23.249669396151763	26.354448419635528	26.99902318788408	23.396858996328632
86-87	21.64013822487308	27.046250841406316	27.587997333800164	23.725613599920443
88-89	22.73478831902018	25.789206246666225	27.269824519011465	24.20618091530213
90-91	22.24623843092003	25.772563044859385	27.549502224435834	24.431696299784747
92-93	22.827003985327682	26.115978049055116	27.151349578742067	23.90566838687513
94-95	21.417252877219195	26.195711314007493	27.907772827281374	24.479262981491935
96-97	21.874427247839055	26.082270428605597	27.367361900464616	24.675940423090736
98-99	21.037305017699325	27.00181760201461	27.203395541923197	24.757481838362867
100	22.050015287052187	26.09702333233887	26.10399348794194	25.748967892667007
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	267.0
1	184.0
2	151.0
3	197.5
4	138.5
5	108.0
6	235.5
7	508.5
8	844.0
9	972.0
10	749.5
11	555.0
12	575.0
13	694.5
14	884.5
15	1278.0
16	1765.0
17	2405.5
18	3379.0
19	4434.0
20	5679.5
21	7019.0
22	8470.0
23	10223.0
24	11784.0
25	13479.0
26	15834.5
27	17602.5
28	21186.5
29	30295.5
30	43279.5
31	55138.5
32	72309.0
33	88256.0
34	95901.5
35	108197.5
36	120135.5
37	140691.0
38	150561.0
39	140303.0
40	127745.5
41	114577.0
42	112173.5
43	120265.5
44	136361.0
45	171015.0
46	202720.5
47	224215.5
48	238731.0
49	230471.0
50	214954.0
51	222470.5
52	230572.5
53	220923.0
54	213390.5
55	182699.0
56	155653.5
57	128244.5
58	98066.5
59	98466.5
60	82562.5
61	52752.5
62	37146.0
63	31259.5
64	22627.5
65	9427.0
66	4198.0
67	2591.0
68	1704.0
69	1063.0
70	598.0
71	345.5
72	186.0
73	80.5
74	29.5
75	14.5
76	8.0
77	2.0
78	0.5
79	0.5
80	0.5
81	0.0
82	0.0
83	0.0
84	0.5
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.008485978205213554
3	0.0
4	0.09055134612681873
5	0.004561470124836342
6	4.1094325448976054E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	2.8766027814283235E-4
14-15	0.009955100340014449
16-17	0.0
18-19	5.1367906811220065E-5
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	1.0273581362244013E-5
60-61	0.0
62-63	0.0
64-65	3.082074408673204E-5
66-67	1.0273581362244013E-4
68-69	0.0
70-71	4.725847426632246E-4
72-73	3.698489290407845E-4
74-75	3.4930176631629644E-4
76-77	8.218865089795211E-5
78-79	1.0273581362244013E-5
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.06696320331910648
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4866852.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.13036931359463
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.61895963747817	45.84461741083018
2	16.057546282387396	20.274376541545095
3	5.728730745504089	10.849706629854522
4	2.486203955596238	6.278198956228412
5	1.2168964508573612	3.841156117951387
6	0.649143495447149	2.4588401163057747
7	0.3800765088013423	1.6796059257635307
8	0.24513845607276213	1.2380545011870294
9	0.16062785328407114	0.9126446129885973
>10	0.44322719807293437	4.008833482349371
>50	0.006496813002758542	0.2842628889219858
>100	0.005724431828470122	0.7413997585547215
>500	6.140858350212597E-4	0.27345973756299374
>1k	4.7237371707821836E-4	0.44919563921218114
>5k	4.72373717078218E-5	0.1942846437938896
>10k+	9.44747434156436E-5	0.671363036950397
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12302	0.2527711958366517	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10211	0.20980707857974723	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6515	0.1338647651500395	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	2.0547162724488027E-5
7	0.0	0.0	0.0	0.0	2.0547162724488027E-5
8	0.0	0.0	0.0	0.0	2.0547162724488027E-5
9	0.0	0.0	0.0	0.0	2.0547162724488027E-5
10-11	0.0	0.0	0.0	0.0	2.0547162724488027E-5
12-13	0.0	0.0	0.0	0.0	1.4383013907141618E-4
14-15	0.0	0.0	0.0	0.0	1.6437730179590421E-4
16-17	0.0	0.0	0.0	0.0	1.6437730179590421E-4
18-19	0.0	0.0	0.0	1.0273581362244013E-5	2.0547162724488026E-4
20-21	0.0	0.0	0.0	5.1367906811220065E-5	2.773866967805883E-4
22-23	0.0	0.0	0.0	1.6437730179590421E-4	2.8766027814283235E-4
24-25	0.0	0.0	0.0	3.390281849540524E-4	2.8766027814283235E-4
26-27	0.0	0.0	0.0	8.01339346255033E-4	2.8766027814283235E-4
28-29	0.0	0.0	0.0	0.003297819617280328	2.8766027814283235E-4
30-31	0.0	0.0	0.0	0.012348844797417304	2.8766027814283235E-4
32-33	0.0	0.0	0.0	0.028426999629329184	2.8766027814283235E-4
34-35	0.0	0.0	0.0	0.053247972200510724	2.8766027814283235E-4
36-37	0.0	0.0	0.0	0.09609908006243051	2.8766027814283235E-4
38-39	0.0	0.0	0.0	0.1613671424567667	3.2875460359180843E-4
40-41	0.0	0.0	0.0	0.2332616648297503	3.2875460359180843E-4
42-43	0.0	0.0	0.0	0.309830666722555	3.390281849540524E-4
44-45	0.0	0.0	0.0	0.4021901631691286	3.4930176631629644E-4
46-47	0.0	0.0	0.0	0.5203671695790215	3.5957534767854047E-4
48-49	0.0	0.0	0.0	0.6560709057929026	3.801225104030285E-4
50-51	0.0	0.0	0.0	0.800116790072926	3.903960917652725E-4
52-53	0.0	0.0	0.0	0.9434537972389545	3.903960917652725E-4
54-55	0.0	0.0	0.0	1.099170469946487	4.109432544897605E-4
56-57	0.0	0.0	0.0	1.2933925255997099	4.2121683585200455E-4
58-59	0.0	0.0	0.0	1.5276301806588735	4.3149041721424853E-4
60-61	0.0	0.0	0.0	1.773744095772791	4.3149041721424853E-4
62-63	0.0	0.0	0.0	2.03634710897311	4.520375799387366E-4
64-65	0.0	0.0	0.0	2.309233977117036	4.520375799387366E-4
66-67	0.0	0.0	0.0	2.617482512309805	4.520375799387366E-4
68-69	0.0	0.0	0.0	2.965736373327153	4.520375799387366E-4
70-71	0.0	0.0	0.0	3.3504922689245533	4.623111613009806E-4
72-73	0.0	0.0	0.0	3.754819337017029	4.8285832402546864E-4
74-75	0.0	0.0	0.0	4.142482656139944	5.034054867499567E-4
76-77	0.0	0.0	0.0	4.590564907254217	5.342262308366887E-4
78-79	0.0	0.0	0.0	5.069087779944819	5.444998121989327E-4
80-81	0.0	0.0	0.0	5.590924071658641	5.650469749234207E-4
82-83	0.0	0.0	0.0	6.139060731659808	6.369620444591288E-4
84-85	0.0	0.0	0.0	6.672804104172471	6.369620444591288E-4
86-87	0.0	0.0	0.0	7.2409126063418405	6.883299512703488E-4
88	0.0	0.0	0.0	7.7034600600141525	7.396978580815689E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	5455	0.0	45.78026	94
GTACTAG	5900	0.0	35.882427	1
GTATCAA	19620	0.0	34.864765	1
GGTATCA	12355	0.0	34.84173	1
CTGTGCG	8070	0.0	32.967064	9
TAGTACT	8250	0.0	32.5014	4
TAGGCAT	7645	0.0	31.785433	5
ACTGTGC	8685	0.0	31.656685	8
GTACTGT	8850	0.0	30.322382	6
TACTGTG	9875	0.0	29.888042	7
CAGTACG	685	0.0	29.506336	9
CAACGCA	22350	0.0	29.378796	5
GAACCGT	515	0.0	29.202044	6
TATTCGC	390	0.0	28.92571	9
ATCAACG	22725	0.0	28.91836	3
CTAGTAC	9635	0.0	28.659428	3
CTAGGCA	8680	0.0	28.506714	4
TCTAACG	2615	0.0	28.245504	2
TATTCTC	2880	0.0	28.070557	5
CCGTGTT	500	0.0	27.262484	9
>>END_MODULE