FastQCFastQC Report
Mon 16 Jan 2017
ERR1146638_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146638_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2750706
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC28800.040.73247594
GTACTAG34550.032.9348141
CTGTGCG40700.031.981949
TAGTACT41550.031.444774
GCATCGT33350.029.7307628
TAGGCAT40700.029.328535
TACGCGG650.00615888528.9199545
GTACTGT47100.028.6338946
ACTGTGC48350.028.379578
CAACGCA47400.027.6615725
TACTGTG50850.027.446377
GGTATCA41450.027.3388611
CTAGTAC50300.027.2828393
CTAGGCA47200.026.6850174
ATCAACG49800.026.4242943
GTATCAA57050.026.209541
CTAACGC18750.025.817293
TCTAACG19400.025.437252
CAGTACG5350.024.5936899
CGCCTAC20000.024.4355877
Produced by FastQC (version 0.11.5)