##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146638_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2750706 Sequences flagged as poor quality 0 Sequence length 100 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.486732497038943 31.0 31.0 34.0 28.0 34.0 2 31.6322634988981 33.0 31.0 34.0 28.0 34.0 3 31.751845889746125 33.0 31.0 34.0 28.0 34.0 4 35.198439600597084 37.0 35.0 37.0 32.0 37.0 5 35.34366558985221 37.0 35.0 37.0 33.0 37.0 6 35.44291901788123 37.0 35.0 37.0 33.0 37.0 7 35.39771971268467 37.0 35.0 37.0 32.0 37.0 8 35.37469216993746 37.0 35.0 37.0 32.0 37.0 9 36.93943482146038 39.0 37.0 39.0 33.0 39.0 10-11 36.793057127879166 39.0 37.0 39.0 32.0 39.0 12-13 36.740953049871564 39.0 37.0 39.0 32.0 39.0 14-15 37.962067920017624 40.0 38.0 41.0 32.0 41.0 16-17 37.966410805080585 40.0 38.0 41.0 32.0 41.0 18-19 37.91697204281374 40.0 38.0 41.0 32.0 41.0 20-21 37.969921721914304 40.0 38.0 41.0 32.0 41.0 22-23 37.89259920907578 40.0 38.0 41.0 32.0 41.0 24-25 37.833353328200104 40.0 37.5 41.0 32.0 41.0 26-27 37.854356845115404 40.0 37.0 41.0 32.0 41.0 28-29 37.8243812679363 40.0 37.0 41.0 32.0 41.0 30-31 37.68099371579515 40.0 37.0 41.0 31.0 41.0 32-33 37.670304823561665 40.0 37.0 41.0 31.0 41.0 34-35 37.60483563128884 40.0 37.0 41.0 31.0 41.0 36-37 37.486484924234 40.0 37.0 41.0 31.0 41.0 38-39 37.386124144128814 40.0 37.0 41.0 31.0 41.0 40-41 37.296627120455625 40.0 36.0 41.0 30.5 41.0 42-43 37.124773603576685 40.0 36.0 41.0 30.0 41.0 44-45 36.97365930782861 39.5 36.0 41.0 30.0 41.0 46-47 36.9682521505388 39.0 35.5 41.0 30.0 41.0 48-49 36.81346916028103 39.0 35.0 41.0 30.0 41.0 50-51 36.626657301798154 39.0 35.0 41.0 30.0 41.0 52-53 36.406850641253556 39.0 35.0 41.0 29.0 41.0 54-55 36.162383039117955 38.0 35.0 40.0 29.0 41.0 56-57 35.91228179238348 38.0 34.5 40.0 29.0 41.0 58-59 35.59745189053283 37.0 34.0 40.0 28.0 41.0 60-61 35.324101158029976 37.0 34.0 40.0 28.0 41.0 62-63 34.990961774904335 36.0 34.0 39.5 28.0 41.0 64-65 34.815173450016104 36.0 34.0 39.0 28.0 41.0 66-67 34.533529210319095 35.0 33.5 39.0 27.5 41.0 68-69 34.37046525510178 35.0 34.0 38.5 28.0 40.5 70-71 34.24368416690115 35.0 34.0 37.5 28.0 40.0 72-73 33.98767570943605 35.0 33.5 37.0 27.5 39.5 74-75 33.71146607452778 35.0 33.0 36.5 27.0 39.0 76-77 33.43996268594317 35.0 33.0 36.0 27.0 39.0 78-79 33.18156466012725 35.0 33.0 36.0 26.0 38.0 80-81 32.99617661793009 35.0 33.0 35.0 26.0 37.0 82-83 32.762888327578445 35.0 33.0 35.0 26.0 37.0 84-85 32.60733299014871 35.0 33.0 35.0 26.0 36.0 86-87 32.45698049882466 35.0 33.0 35.0 26.0 36.0 88-89 32.348793364321736 35.0 33.0 35.0 25.0 36.0 90-91 32.22335429522457 35.0 33.0 35.0 25.0 35.5 92-93 32.08198767879955 35.0 32.0 35.0 25.0 35.0 94-95 32.02371700210782 35.0 32.0 35.0 25.0 35.0 96-97 31.922895067666264 35.0 32.0 35.0 25.0 35.0 98-99 31.831807361455567 35.0 32.0 35.0 24.0 35.0 100 30.857799415859056 34.0 30.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22431473714290817 1101 2 0.4056230756633674 1101 3 0.41539958596940707 1101 4 0.41500605041687066 1101 5 0.3271803319005784 1101 6 0.3481154468644121 1101 7 0.4071880908620642 1101 8 0.3822728336345449 1101 9 0.5261882998881546 1101 10-11 0.5248227366072342 1101 12-13 0.47013880838152033 1101 14-15 0.6502065908188541 1101 16-17 0.6127426781598047 1101 18-19 0.5898272518372281 1101 20-21 0.6307724481757688 1101 22-23 0.651498393410165 1101 24-25 0.646594948214279 1101 26-27 0.5904489452332555 1101 28-29 0.5773607155467673 1101 30-31 0.6618654725942505 1101 32-33 0.6078541043025822 1101 34-35 0.6519433747383161 1101 36-37 0.6056348580197621 1101 38-39 0.5720727776330179 1101 40-41 0.5712173041283464 1101 42-43 0.5806766076557892 1101 44-45 0.5992329519919508 1101 46-47 0.6081214864722568 1101 48-49 0.5617527492197567 1101 50-51 0.5555570313169795 1101 52-53 0.4767391247638173 1101 54-55 0.5807744145580074 1101 56-57 0.5222517189763707 1101 58-59 0.4943260454744305 1101 60-61 0.5880345883251366 1101 62-63 0.5958696504355387 1101 64-65 0.5468424223085293 1101 66-67 0.5849618558703611 1101 68-69 0.564680945780978 1101 70-71 0.5212232942164263 1101 72-73 0.5669401556350664 1101 74-75 0.45411862715977946 1101 76-77 0.4535171227455521 1101 78-79 0.3629521409269998 1101 80-81 0.35692861781682694 1101 82-83 0.39849793987688287 1101 84-85 0.34172138830486887 1101 86-87 0.34050322768288055 1101 88-89 0.3964316353702131 1101 90-91 0.38784676315486166 1101 92-93 0.3798376231019205 1101 94-95 0.3541001120371803 1101 96-97 0.32202380466489444 1101 98-99 0.2472259112386226 1101 100 0.3614645149898621 1102 1 0.27316883452126817 1102 2 0.3536634045191036 1102 3 0.3476271882820505 1102 4 0.4214462085578816 1102 5 0.2831848076649308 1102 6 0.3868388545222885 1102 7 0.2853450764477614 1102 8 0.34439987617679435 1102 9 0.4263494194089503 1102 10-11 0.5384424960373977 1102 12-13 0.440202948843357 1102 14-15 0.6101206762331941 1102 16-17 0.5498453453775269 1102 18-19 0.5792173639762126 1102 20-21 0.5535281566993646 1102 22-23 0.5497833250867927 1102 24-25 0.6156949452657514 1102 26-27 0.5568763397496923 1102 28-29 0.5833489744114715 1102 30-31 0.6226379417459071 1102 32-33 0.5661527466307348 1102 34-35 0.566818707009844 1102 36-37 0.659824540273469 1102 38-39 0.6409400659225142 1102 40-41 0.5879213979334921 1102 42-43 0.5862609432801449 1102 44-45 0.6096138292437558 1102 46-47 0.5080708367717861 1102 48-49 0.49167319145886523 1102 50-51 0.47044029712570534 1102 52-53 0.42331499761532854 1102 54-55 0.4380983062740995 1102 56-57 0.43163354525414377 1102 58-59 0.45836007297769754 1102 60-61 0.39907734746694956 1102 62-63 0.4054152823923829 1102 64-65 0.43235685784797795 1102 66-67 0.43422858463934233 1102 68-69 0.30484660803267616 1102 70-71 0.2946547776904396 1102 72-73 0.32617785390478815 1102 74-75 0.2758106295046048 1102 76-77 0.2983113980050547 1102 78-79 0.3016448264305964 1102 80-81 0.3701099712307965 1102 82-83 0.3511137332880736 1102 84-85 0.2773908963855902 1102 86-87 0.30557529918316817 1102 88-89 0.42083250963352015 1102 90-91 0.3815260453533966 1102 92-93 0.45721291164529987 1102 94-95 0.46174003339645964 1102 96-97 0.4863702136336201 1102 98-99 0.32116422904888253 1102 100 0.4205984775755489 1103 1 0.2346391656956186 1103 2 0.3543596031903533 1103 3 0.3264961554986847 1103 4 0.3922302742339383 1103 5 0.22086416084846405 1103 6 0.3344179007988899 1103 7 0.35382875854190843 1103 8 0.32016075491258533 1103 9 0.41113044462586856 1103 10-11 0.5003261514207011 1103 12-13 0.43167748265444317 1103 14-15 0.5721124107229727 1103 16-17 0.56263605565114 1103 18-19 0.6119909909941583 1103 20-21 0.47699947498201567 1103 22-23 0.590168326543477 1103 24-25 0.5506272447082452 1103 26-27 0.4985548937991666 1103 28-29 0.530459150331744 1103 30-31 0.5008905871730249 1103 32-33 0.5082886356996283 1103 34-35 0.4983165501149003 1103 36-37 0.539345951015342 1103 38-39 0.6024816787829792 1103 40-41 0.5374008224839315 1103 42-43 0.5497182134094913 1103 44-45 0.5257848923854596 1103 46-47 0.5512448026790508 1103 48-49 0.5018516196848992 1103 50-51 0.4546668652649686 1103 52-53 0.5299927280600158 1103 54-55 0.48898417753778745 1103 56-57 0.4507259279020275 1103 58-59 0.5436347010437288 1103 60-61 0.4933383986545081 1103 62-63 0.4818856787002943 1103 64-65 0.4904984255897489 1103 66-67 0.4575107294839711 1103 68-69 0.5145615592050277 1103 70-71 0.5205214832505618 1103 72-73 0.4727407009160203 1103 74-75 0.47281394376820884 1103 76-77 0.4053225575152055 1103 78-79 0.4439003664663659 1103 80-81 0.4087700898031059 1103 82-83 0.4837950958725017 1103 84-85 0.48807886387870525 1103 86-87 0.44307867608456064 1103 88-89 0.5287735268688323 1103 90-91 0.5690901364765963 1103 92-93 0.42113427364047595 1103 94-95 0.44304460025764314 1103 96-97 0.4318445631227945 1103 98-99 0.39152028807132666 1103 100 0.43841567286095184 1104 1 0.24468438441611084 1104 2 0.34763918138505545 1104 3 0.30948096989850526 1104 4 0.40459387376103706 1104 5 0.24644211918266734 1104 6 0.2715481900941086 1104 7 0.2457213849791131 1104 8 0.31250968646023125 1104 9 0.33594284314523293 1104 10-11 0.39157041363409206 1104 12-13 0.27563048252802247 1104 14-15 0.4504661873982485 1104 16-17 0.43002776194234826 1104 18-19 0.3349782514298525 1104 20-21 0.34868059755600456 1104 22-23 0.5134498936865413 1104 24-25 0.45982845751377255 1104 26-27 0.4754983290136039 1104 28-29 0.43290576271618164 1104 30-31 0.4081635907197807 1104 32-33 0.547928623691817 1104 34-35 0.511973995555735 1104 36-37 0.47573007583071103 1104 38-39 0.3713807032099794 1104 40-41 0.4497269078917583 1104 42-43 0.5004501505257792 1104 44-45 0.4582747139524912 1104 46-47 0.44168977943141385 1104 48-49 0.4474260860352288 1104 50-51 0.34606390177308555 1104 52-53 0.4104038103320562 1104 54-55 0.389683356715679 1104 56-57 0.45963645775704975 1104 58-59 0.4222460527091556 1104 60-61 0.4591968440051559 1104 62-63 0.35983157683401146 1104 64-65 0.3837559318108674 1104 66-67 0.34136767509344423 1104 68-69 0.4386335583122616 1104 70-71 0.32598050823760616 1104 72-73 0.31226695235780966 1104 74-75 0.3040269675503495 1104 76-77 0.2226397091270229 1104 78-79 0.20396751303374572 1104 80-81 0.22090115120560938 1104 82-83 0.30290909555707657 1104 84-85 0.22878567765342694 1104 86-87 0.2736217166389352 1104 88-89 0.25636337030194767 1104 90-91 0.2566704098972892 1104 92-93 0.12866641698147419 1104 94-95 0.18309028430146412 1104 96-97 0.13130293501001944 1104 98-99 0.11502393385898202 1104 100 0.22478985293448872 1105 1 0.12152549980217486 1105 2 0.23801908496967528 1105 3 0.20405445269736688 1105 4 0.3340446530102881 1105 5 0.2214123457339241 1105 6 0.24071952053160572 1105 7 0.27702506451963416 1105 8 0.29318003274772764 1105 9 0.4418158616951118 1105 10-11 0.34154961438061804 1105 12-13 0.2652087716408502 1105 14-15 0.40607323248283933 1105 16-17 0.3877127247245369 1105 18-19 0.36216745316414034 1105 20-21 0.36351893756678777 1105 22-23 0.4006653394209678 1105 24-25 0.5279042535269056 1105 26-27 0.4553788658110136 1105 28-29 0.4511298036015674 1105 30-31 0.5570027273660543 1105 32-33 0.408226904260097 1105 34-35 0.45343714674703506 1105 36-37 0.4204753331592386 1105 38-39 0.492077606690998 1105 40-41 0.5192704967828661 1105 42-43 0.44958593752856757 1105 44-45 0.47130315753391017 1105 46-47 0.444639619502027 1105 48-49 0.30089193229581923 1105 50-51 0.41635072267545326 1105 52-53 0.47048333736184844 1105 54-55 0.37163056837097486 1105 56-57 0.49655192043604046 1105 58-59 0.49449655602700204 1105 60-61 0.5211828354268775 1105 62-63 0.47230052852754767 1105 64-65 0.3941288568042651 1105 66-67 0.34756428666216266 1105 68-69 0.3991894605220452 1105 70-71 0.4048807497177407 1105 72-73 0.36175953890960244 1105 74-75 0.34665258821532774 1105 76-77 0.3713949260054079 1105 78-79 0.4614885712740673 1105 80-81 0.2795753232708478 1105 82-83 0.33085959815216626 1105 84-85 0.31485041874287134 1105 86-87 0.36351344006185826 1105 88-89 0.2996318404807212 1105 90-91 0.33954539674717665 1105 92-93 0.33211001848063404 1105 94-95 0.2599961263219015 1105 96-97 0.15508194254031338 1105 98-99 0.2293832887448346 1105 100 0.35847083093305443 1106 1 0.10342098553020307 1106 2 0.11522991762315016 1106 3 0.14109811885748513 1106 4 0.26629449811054684 1106 5 0.19043182260304548 1106 6 0.2033702138838862 1106 7 0.16992928334181556 1106 8 0.16658021107917875 1106 9 0.2545346328096443 1106 10-11 0.38458718542397463 1106 12-13 0.21526964583262043 1106 14-15 0.3664763716311228 1106 16-17 0.2819861676647264 1106 18-19 0.33002849939141754 1106 20-21 0.2850119390165773 1106 22-23 0.2777962664400633 1106 24-25 0.35545692259913153 1106 26-27 0.2954177237314468 1106 28-29 0.2769082631335422 1106 30-31 0.41049864769801303 1106 32-33 0.3253235448112406 1106 34-35 0.3733469711419133 1106 36-37 0.31677847112415236 1106 38-39 0.3491408741455615 1106 40-41 0.2980817916137539 1106 42-43 0.2733489196091483 1106 44-45 0.22313963717314778 1106 46-47 0.20700057881541056 1106 48-49 0.11170494541378417 1106 50-51 0.29001121184959544 1106 52-53 0.20089335953897347 1106 54-55 0.24938666650359664 1106 56-57 0.23979449452567536 1106 58-59 0.24898910814403052 1106 60-61 0.2367993432990474 1106 62-63 0.22321749020167658 1106 64-65 0.19440908948304525 1106 66-67 0.23035504293022058 1106 68-69 0.17306251821483443 1106 70-71 0.17178239984089316 1106 72-73 0.09655750633084637 1106 74-75 0.17142567861715463 1106 76-77 0.20862427654551396 1106 78-79 0.11632274151982358 1106 80-81 0.16616449813113832 1106 82-83 0.14108009749172368 1106 84-85 0.1743506931563985 1106 86-87 0.23851081196497148 1106 88-89 0.3113656181146567 1106 90-91 0.24624744018299083 1106 92-93 0.2624934265093657 1106 94-95 0.20434865918115008 1106 96-97 0.21854878779119247 1106 98-99 0.2810399548565883 1106 100 0.36630462767310945 1107 1 0.081309022432702 1107 2 0.1308523369863046 1107 3 0.12229071512031453 1107 4 0.2454748332033745 1107 5 0.1525218180339536 1107 6 0.162994194384396 1107 7 0.1061143456791882 1107 8 0.13204828753843856 1107 9 0.22937140360239994 1107 10-11 0.29790546025731857 1107 12-13 0.2632875355597051 1107 14-15 0.36340316006322837 1107 16-17 0.2443605774020412 1107 18-19 0.15057607568358833 1107 20-21 0.24519098473337664 1107 22-23 0.31304049688853297 1107 24-25 0.3643348977866765 1107 26-27 0.24716921675313586 1107 28-29 0.25634566106962353 1107 30-31 0.33705339428144754 1107 32-33 0.2596181418102219 1107 34-35 0.30767821208831947 1107 36-37 0.26184971993234996 1107 38-39 0.33833964692982477 1107 40-41 0.3332867773880679 1107 42-43 0.33405613493681585 1107 44-45 0.42285362152038886 1107 46-47 0.34690889413943893 1107 48-49 0.306102255338935 1107 50-51 0.31480960877332365 1107 52-53 0.2857835930912458 1107 54-55 0.24039268137086367 1107 56-57 0.29185663120561145 1107 58-59 0.2831273576340294 1107 60-61 0.2502446621721006 1107 62-63 0.22842420355986803 1107 64-65 0.2333237343330552 1107 66-67 0.21751309265868457 1107 68-69 0.2014039523736102 1107 70-71 0.2599681924857151 1107 72-73 0.31369454803166263 1107 74-75 0.25400602850782406 1107 76-77 0.25633875789456084 1107 78-79 0.2365525265384889 1107 80-81 0.10534045465213637 1107 82-83 0.17171554795687172 1107 84-85 0.16365428399440418 1107 86-87 0.19546767414901467 1107 88-89 0.19248233744457366 1107 90-91 0.20827536883214037 1107 92-93 0.20565431003642942 1107 94-95 0.19079221037824112 1107 96-97 0.2956332468885421 1107 98-99 0.27102502376298787 1107 100 0.3977744146507973 1108 1 -0.011332983216416181 1108 2 -0.012221819770132925 1108 3 -0.06909518726754627 1108 4 0.1596185724790118 1108 5 0.0547247356089855 1108 6 0.06753998804016703 1108 7 -0.04385119878042332 1108 8 0.08504614325288884 1108 9 0.03965363927427035 1108 10-11 0.17149431265685422 1108 12-13 0.01920669092751126 1108 14-15 0.15485763238790184 1108 16-17 0.10591258446383733 1108 18-19 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-0.9990096491269895 2215 26-27 -0.913533744129083 2215 28-29 -0.8115964500662898 2215 30-31 -3.7339103993528653 2215 32-33 -1.4679576041827929 2215 34-35 -1.2071976861394162 2215 36-37 -0.961792060780347 2215 38-39 -0.8339406354482435 2215 40-41 -0.9619427910268996 2215 42-43 -0.9283745189236043 2215 44-45 -0.8123911670231223 2215 46-47 -1.0649179135461395 2215 48-49 -1.0179423940860488 2215 50-51 -0.9256293913072469 2215 52-53 -0.929946072107164 2215 54-55 -0.885458464033519 2215 56-57 -0.911055079233499 2215 58-59 -0.8732770703112323 2215 60-61 -0.907872977453188 2215 62-63 -0.9314543986978805 2215 64-65 -0.9676713353393183 2215 66-67 -0.8393668445084188 2215 68-69 -0.8219058115650739 2215 70-71 -0.8887857193870872 2215 72-73 -0.7157059139657704 2215 74-75 -0.9112556996442791 2215 76-77 -0.7995174033099772 2215 78-79 -0.6897986220418559 2215 80-81 -0.7609068988474448 2215 82-83 -0.7123976265921854 2215 84-85 -0.7638406586949777 2215 86-87 -0.7502856280494328 2215 88-89 -0.7059798868716811 2215 90-91 -0.7715152802944232 2215 92-93 -0.7280732463194468 2215 94-95 -0.7793066669673365 2215 96-97 -0.942659411956928 2215 98-99 -0.9283327788596445 2215 100 -0.3684525792244884 2216 1 -0.4490438191943724 2216 2 -0.7165969029395889 2216 3 -0.7649437113951656 2216 4 -0.482346596935777 2216 5 -0.46781663330915535 2216 6 -0.4694109983216066 2216 7 -0.5779715485528953 2216 8 -0.6200540357136006 2216 9 -0.8059937025066191 2216 10-11 -1.0677239011521706 2216 12-13 -0.6091421352611235 2216 14-15 -0.8353223601356277 2216 16-17 -0.846859185697383 2216 18-19 -1.0813169113282086 2216 20-21 -1.1186932161835088 2216 22-23 -1.0606482070343048 2216 24-25 -1.02283697919637 2216 26-27 -1.3592284459334962 2216 28-29 -1.2302991187088068 2216 30-31 -1.33846575646038 2216 32-33 -1.63066240283095 2216 34-35 -1.2517336220237851 2216 36-37 -1.3540354952676168 2216 38-39 -1.3448120514858601 2216 40-41 -1.200745071557435 2216 42-43 -1.2326584024877292 2216 44-45 -1.0897592024257392 2216 46-47 -1.2459110058581047 2216 48-49 -1.2820373259532971 2216 50-51 -1.3359444931632751 2216 52-53 -1.2643351083316858 2216 54-55 -1.2576100142519522 2216 56-57 -1.2801282296801801 2216 58-59 -1.2007280139413297 2216 60-61 -1.2557675905834245 2216 62-63 -1.2815094716244317 2216 64-65 -1.2313321218234563 2216 66-67 -1.17818663866111 2216 68-69 -1.2205409245472794 2216 70-71 -1.107429081776374 2216 72-73 -1.0339114159611853 2216 74-75 -1.0869617463674004 2216 76-77 -1.1184936088396569 2216 78-79 -0.965018647150174 2216 80-81 -1.0157049542848284 2216 82-83 -1.1185736410244829 2216 84-85 -1.0968323747169748 2216 86-87 -1.082124971541063 2216 88-89 -1.0637373327774782 2216 90-91 -1.1640416557548363 2216 92-93 -1.1973998557312981 2216 94-95 -1.1270059568348465 2216 96-97 -1.094677106716862 2216 98-99 -1.2018356654825268 2216 100 -0.7267227114952384 >>END_MODULE >>Per sequence quality scores pass #Quality Count 1 3.0 2 5.0 3 8.0 4 22.0 5 22.0 6 22.0 7 92.0 8 256.0 9 498.0 10 636.0 11 1070.0 12 1771.0 13 2643.0 14 3739.0 15 5477.0 16 6889.0 17 8167.0 18 9409.0 19 10718.0 20 12279.0 21 13900.0 22 16335.0 23 18798.0 24 21710.0 25 25444.0 26 28760.0 27 32648.0 28 38023.0 29 45976.0 30 54477.0 31 65800.0 32 82297.0 33 106572.0 34 152379.0 35 223373.0 36 371876.0 37 672764.0 38 579522.0 39 136241.0 40 85.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.648742083001714 19.08285767081292 11.77695780656407 24.491442439621295 2 16.443264373599796 19.47090231791089 38.99470422404121 25.091129084448106 3 19.555280049591072 24.9882657485289 28.40474169194999 27.05171250993003 4 12.747186823974257 15.890163427802722 38.75351306149903 32.609136686723986 5 13.73697507190273 38.253560780210506 34.39831657996409 13.611147567922677 6 31.63867687808609 36.97658659093173 17.37548197768815 14.009254553294026 7 28.02529987065045 31.382426056627327 22.33657349475731 18.255700577964916 8 27.049985494485362 34.169902838500185 19.373478102489642 19.40663356452482 9 26.41786139633227 14.818053057194335 20.28718591539874 38.47689963107465 10-11 26.491138682763953 25.060752905224014 26.898908874368498 21.549199537643535 12-13 26.54943826086356 22.77292476801801 26.819805951106602 23.857831020011826 14-15 23.861099407023882 24.158150958610186 24.30774918829782 27.673000446068112 16-17 23.32681133576981 26.86655825754329 25.321582831368556 24.485047575318347 18-19 22.63843813995795 27.479951240952655 26.74649330762807 23.13511731146132 20-21 24.536909374839244 26.77692196810494 25.957341766652675 22.72882689040314 22-23 24.810005415043353 26.253707959568402 25.687409669751432 23.248876955636817 24-25 24.31303025775203 26.144077054765262 26.335794191008944 23.207098496473762 26-27 24.176865302388958 26.619048190348842 25.17598804339251 24.02809846386969 28-29 23.29215195543075 26.843782220289715 26.167056158010844 23.69700966626869 30-31 23.89324355767553 26.708542987609796 26.2627246815194 23.135488773195277 32-33 24.035217933413207 26.65325834416306 25.38495755636958 23.926566166054148 34-35 24.12307354481206 26.807018283725952 26.011505424876724 23.058402746585262 36-37 23.786071638729435 26.155802175000996 25.472358203463646 24.58576798280592 38-39 23.91026852908883 26.51232121692468 25.98544285849324 23.59196739549325 40-41 23.758160875243927 25.594031733682108 26.722225302417062 23.925582088656896 42-43 23.572913591271426 25.813595052801052 26.2645419230607 24.348949432866817 44-45 24.013028238151684 26.307532597866324 26.131762284650463 23.547676879331533 46-47 23.71877557172026 26.398532777525375 25.578001552960743 24.30469009779362 48-49 24.342386828282976 26.217549320306095 25.849640108576487 23.590423742834442 50-51 23.796403678024337 25.92371937365886 26.25741916165814 24.022457786658663 52-53 23.6864741367508 26.218736039932633 25.92912117552108 24.16566864779549 54-55 23.382708395639238 26.160207911584855 25.989802587622933 24.467281105152974 56-57 24.478987520715787 25.880970855006584 25.47332708435897 24.166714539918658 58-59 23.281702147464397 26.349030549476797 26.379427857933308 23.989839445125497 60-61 23.457092223595005 26.510783938008082 25.944389945220404 24.087733893176512 62-63 23.76802704900405 26.192497097256762 25.487240018826245 24.552235834912945 64-65 23.076134384181575 26.319574551167406 26.03481731652664 24.569473748124373 66-67 23.883044188841076 26.171585122647805 25.755802432776857 24.189568255734258 68-69 23.403158835020076 25.974643083199815 26.26343766439768 24.358760417382427 70-71 23.19098558162068 25.83464901158251 26.033505030481013 24.940860376315793 72-73 23.67981082619384 25.787474324996683 26.53334237415817 23.999372474651302 74-75 23.960642844209683 25.939517205121287 26.07186149515598 24.02797845551305 76-77 23.694716548609264 26.01653742918146 25.60385612782013 24.684889894389144 78-79 23.790341126520133 26.042781477781645 25.480439467744826 24.686437927953396 80-81 23.325003753946188 26.43950656604273 26.104178278449368 24.131311401561714 82-83 24.06350980318018 25.746290904754566 26.961544949784333 23.228654342280922 84-85 23.958394687643843 25.93347629342158 26.208761722763597 23.899367296170983 86-87 22.95381380126884 26.517302804671722 26.506468271086575 24.022415122972866 88-89 24.055134055821203 25.288620670599048 26.28805859015974 24.36818668342001 90-91 23.61526258935652 25.774526161857413 25.910853819446842 24.699357429339226 92-93 23.853513709843664 25.71110599683181 26.370375379617077 24.065004913707444 94-95 23.083756753499387 25.786119962472533 26.91365282582175 24.216470458206334 96-97 23.362266643834147 25.691347238628552 26.49786599565237 24.448520121884933 98-99 22.954613666164388 25.995699999036525 26.350258292735646 24.69942804206344 100 23.64377321519757 25.54687724953465 25.697335238842946 25.11201429642484 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 79.0 1 60.5 2 50.5 3 61.0 4 61.0 5 57.5 6 59.0 7 64.5 8 72.5 9 81.0 10 72.5 11 70.5 12 86.0 13 87.5 14 95.5 15 113.0 16 122.0 17 138.0 18 188.5 19 269.0 20 363.5 21 483.5 22 657.5 23 927.5 24 1260.5 25 1708.5 26 2365.5 27 3135.5 28 4143.0 29 5824.5 30 8292.0 31 11194.5 32 15489.5 33 20617.5 34 25932.0 35 32220.5 36 39683.0 37 50862.0 38 60256.5 39 67472.0 40 71689.0 41 73325.5 42 78627.0 43 87166.5 44 98582.5 45 115978.5 46 132169.5 47 143924.5 48 152933.0 49 151895.5 50 144924.0 51 147524.0 52 149019.5 53 142150.0 54 133556.0 55 114747.5 56 99745.0 57 82470.0 58 64429.5 59 59657.0 60 48478.5 61 33064.5 62 23875.5 63 19309.5 64 13696.0 65 6140.5 66 2841.5 67 1674.5 68 954.0 69 513.0 70 278.0 71 157.5 72 102.0 73 70.0 74 47.0 75 34.0 76 28.0 77 27.0 78 19.5 79 11.5 80 9.5 81 10.5 82 9.5 83 8.5 84 7.5 85 8.5 86 11.0 87 7.0 88 7.0 89 8.5 90 5.0 91 2.5 92 2.0 93 3.0 94 3.5 95 4.5 96 4.0 97 2.5 98 2.0 99 2.5 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.056640004420683274 2 0.007707112283173847 3 0.007670757979951329 4 0.009306701624964645 5 0.004180744870589587 6 0.0021812581933510888 7 7.270860644503629E-4 8 0.0010179204902305082 9 5.089602451152541E-4 10-11 5.271373967265131E-4 12-13 2.726572741688861E-4 14-15 1.817715161125907E-4 16-17 5.453145483377722E-4 18-19 0.0013451092192331715 20-21 0.0010724519450642854 22-23 0.0012905777643993943 24-25 0.0014178178256782077 26-27 0.003380950199694187 28-29 0.003998973354476997 30-31 0.005307728270487649 32-33 0.006380180215551934 34-35 0.003290064441637892 36-37 0.008161541073455323 38-39 0.0060348143349380125 40-41 0.005762157060769126 42-43 0.013887343831001932 44-45 0.019122363495044547 46-47 0.01728647118230738 48-49 0.02075830714005786 50-51 0.01743188839519745 52-53 0.02452097752358849 54-55 0.018122620156425295 56-57 0.01410546965033704 58-59 0.016813865240414644 60-61 0.014977972927677477 62-63 0.010560925086141522 64-65 0.010651810844197817 66-67 0.012760360431103868 68-69 0.00490783093503995 70-71 0.008125186770232806 72-73 0.007979769557342732 74-75 0.011051708179645516 76-77 0.010124673447471304 78-79 0.012124160124709802 80-81 0.009706598960412345 82-83 0.0061802315478280846 84-85 0.010470039328085226 86-87 0.008779564228238133 88-89 0.007634403676728811 90-91 0.012614943218213796 92-93 0.0086159698637368 94-95 0.006816431854222153 96-97 0.00883409568307191 98-99 0.008779564228238133 100 0.257497529725096 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2750706.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.15412994767729 #Duplication Level Percentage of deduplicated Percentage of total 1 79.18631196653098 58.719920676434526 2 13.89151284188242 20.60226096893554 3 4.00541682387121 8.910545989558758 4 1.4848189147163775 4.404218190025897 5 0.6187844511714877 2.2942711300886343 6 0.3248114444123841 1.4451666034469215 7 0.17960974632834892 0.932316312837122 8 0.0971557598570454 0.576360067328374 9 0.054239004219139526 0.36198415503888204 >10 0.15563062110133283 1.6172104668630733 >50 0.0012191810277017317 0.05940128721403958 >100 4.2827771276630216E-4 0.04849499329709371 >500 6.096716881639685E-5 0.027849158931150916 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.6354303222518144E-5 0.0 10-11 3.6354303222518144E-5 0.0 0.0 3.6354303222518144E-5 0.0 12-13 3.6354303222518144E-5 0.0 0.0 7.270860644503629E-5 5.453145483377722E-5 14-15 3.6354303222518144E-5 0.0 0.0 7.270860644503629E-5 7.270860644503629E-5 16-17 3.6354303222518144E-5 0.0 0.0 7.270860644503629E-5 7.270860644503629E-5 18-19 3.6354303222518144E-5 0.0 0.0 7.270860644503629E-5 7.270860644503629E-5 20-21 3.6354303222518144E-5 0.0 0.0 1.272400612788135E-4 7.270860644503629E-5 22-23 3.6354303222518144E-5 0.0 0.0 2.1812581933510888E-4 7.270860644503629E-5 24-25 3.6354303222518144E-5 0.0 0.0 3.8172018383644053E-4 7.270860644503629E-5 26-27 3.6354303222518144E-5 0.0 0.0 6.907317612278448E-4 7.270860644503629E-5 28-29 3.6354303222518144E-5 0.0 0.0 0.0032900644416378926 7.270860644503629E-5 30-31 3.6354303222518144E-5 0.0 0.0 0.013523800798776752 7.270860644503629E-5 32-33 3.6354303222518144E-5 0.0 0.0 0.03141011798425568 1.4541721289007258E-4 34-35 3.6354303222518144E-5 0.0 0.0 0.059348400010760874 1.8177151611259074E-4 36-37 3.6354303222518144E-5 0.0 0.0 0.10848124081599414 1.8177151611259074E-4 38-39 3.6354303222518144E-5 0.0 0.0 0.18677023280568697 1.9994866772384982E-4 40-41 3.6354303222518144E-5 0.0 0.0 0.26994887857880845 2.1812581933510888E-4 42-43 3.6354303222518144E-5 0.0 0.0 0.3544726335711632 2.1812581933510888E-4 44-45 3.6354303222518144E-5 0.0 0.0 0.4598274043100208 2.1812581933510888E-4 46-47 3.6354303222518144E-5 0.0 0.0 0.5912482104594239 2.1812581933510888E-4 48-49 3.6354303222518144E-5 0.0 0.0 0.745117798848732 2.1812581933510888E-4 50-51 3.6354303222518144E-5 0.0 0.0 0.90649455085349 2.1812581933510888E-4 52-53 3.6354303222518144E-5 0.0 0.0 1.0644176440521087 2.1812581933510888E-4 54-55 3.6354303222518144E-5 0.0 0.0 1.244480507913241 2.1812581933510888E-4 56-57 3.6354303222518144E-5 0.0 0.0 1.461970126941956 2.1812581933510888E-4 58-59 5.453145483377722E-5 0.0 0.0 1.7197221367896098 2.1812581933510888E-4 60-61 7.270860644503629E-5 0.0 0.0 1.9855629791042735 2.1812581933510888E-4 62-63 7.270860644503629E-5 0.0 0.0 2.2678359664755154 2.1812581933510888E-4 64-65 7.270860644503629E-5 0.0 0.0 2.565832189990497 2.1812581933510888E-4 66-67 7.270860644503629E-5 0.0 0.0 2.908198840588562 2.1812581933510888E-4 68-69 7.270860644503629E-5 0.0 0.0 3.288555738054158 2.1812581933510888E-4 70-71 7.270860644503629E-5 0.0 0.0 3.713882908606009 2.1812581933510888E-4 72-73 7.270860644503629E-5 0.0 0.0 4.153497320324309 2.1812581933510888E-4 74-75 7.270860644503629E-5 0.0 0.0 4.584423053572428 2.1812581933510888E-4 76-77 7.270860644503629E-5 0.0 0.0 5.074115517979747 2.1812581933510888E-4 78-79 7.270860644503629E-5 0.0 0.0 5.607124134676697 2.1812581933510888E-4 80-81 7.270860644503629E-5 0.0 0.0 6.178413832666958 2.1812581933510888E-4 82-83 7.270860644503629E-5 0.0 0.0 6.782749592286489 2.1812581933510888E-4 84-85 7.270860644503629E-5 0.0 0.0 7.36874460593026 2.1812581933510888E-4 86-87 7.270860644503629E-5 0.0 0.0 7.986567812045344 2.1812581933510888E-4 88 7.270860644503629E-5 0.0 0.0 8.485603332380851 2.1812581933510888E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCACC 2880 0.0 40.732475 94 GTACTAG 3455 0.0 32.934814 1 CTGTGCG 4070 0.0 31.98194 9 TAGTACT 4155 0.0 31.44477 4 GCATCGT 3335 0.0 29.730762 8 TAGGCAT 4070 0.0 29.32853 5 TACGCGG 65 0.006158885 28.919954 5 GTACTGT 4710 0.0 28.633894 6 ACTGTGC 4835 0.0 28.37957 8 CAACGCA 4740 0.0 27.661572 5 TACTGTG 5085 0.0 27.44637 7 GGTATCA 4145 0.0 27.338861 1 CTAGTAC 5030 0.0 27.282839 3 CTAGGCA 4720 0.0 26.685017 4 ATCAACG 4980 0.0 26.424294 3 GTATCAA 5705 0.0 26.20954 1 CTAACGC 1875 0.0 25.81729 3 TCTAACG 1940 0.0 25.43725 2 CAGTACG 535 0.0 24.593689 9 CGCCTAC 2000 0.0 24.435587 7 >>END_MODULE