FastQCFastQC Report
Mon 16 Jan 2017
ERR1146638_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146638_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2750706
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36080.13116632602684547No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31710.11527949551860504No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC28500.042.9008794
GGTATCA46200.040.8324971
GTATCAA78650.036.8455921
CAACGCA83300.032.442015
CTGTGCG40100.032.2356459
ATCAACG86250.031.5239523
TAGTACT42450.031.2495174
GTGGTAT13550.030.89971
TATCAAC97250.029.7501452
GTACTAG32650.029.5375351
GTACTGT47250.029.0435836
ACTGTGC47850.028.6809648
AACGCAG100200.028.1887556
TAGGCAT42750.027.8143795
CTAGTAC49950.026.8399263
ACGCAGA100850.026.795947
TACTGTG52800.026.7032647
TCTAACG14500.026.604152
CTAACGC14150.026.5953373
CGCAGAG101400.026.5588638
Produced by FastQC (version 0.11.5)