FastQCFastQC Report
Mon 16 Jan 2017
ERR1146637_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146637_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4462132
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT293410.6575556258757024No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA101800.22814206303175252No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG51040.11438478287957415No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG75500.019.861224
GTACCCG7300.018.0365451
TATTCGC2705.467573E-717.4031529
TCTAACG6300.017.1616572
TAGTACT24450.016.9186614
TACCTGG44350.016.6409442
ACATGGG235050.016.4593073
GTGATCG8000.016.4467168
TGATCGC7450.016.3986749
GTACATG262550.016.3880481
GTACTAG17800.016.3790931
CTAACGC6650.016.2584133
CGTTAAC4359.458745E-1116.215121
TACATGG259400.016.1646692
CTAGTAC23450.015.6359293
ATGGGGG51000.015.4841255
GTATAAG19700.015.2767841
TACGGGC10650.015.0069174
CTGTGCG21950.014.9849469
TGCGCGA9000.014.64410894
Produced by FastQC (version 0.11.5)