##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146637_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4462132 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.23181855668994 31.0 31.0 34.0 28.0 34.0 2 31.305401543477423 31.0 31.0 34.0 28.0 34.0 3 31.451319234841105 33.0 31.0 34.0 28.0 34.0 4 35.002352911119615 37.0 35.0 37.0 32.0 37.0 5 34.9845056130119 37.0 35.0 37.0 32.0 37.0 6 35.15454159581115 37.0 35.0 37.0 32.0 37.0 7 35.047835653449965 37.0 35.0 37.0 32.0 37.0 8 35.04940799599832 37.0 35.0 37.0 32.0 37.0 9 36.70723815431727 39.0 37.0 39.0 32.0 39.0 10-11 36.719147931975115 39.0 37.0 39.0 32.5 39.0 12-13 36.690134334887446 39.0 37.0 39.0 32.0 39.0 14-15 37.997342413895424 40.0 38.0 41.0 33.0 41.0 16-17 37.99687481230945 40.0 38.0 41.0 33.0 41.0 18-19 37.988893761995385 40.0 38.0 41.0 32.0 41.0 20-21 38.0891828166446 40.0 38.0 41.0 33.0 41.0 22-23 38.062991861289625 40.0 38.0 41.0 32.0 41.0 24-25 38.06001906711859 40.0 38.0 41.0 32.0 41.0 26-27 38.0979765950447 40.0 38.0 41.0 32.5 41.0 28-29 38.07133921632081 40.0 38.0 41.0 32.5 41.0 30-31 38.037422805959125 40.0 38.0 41.0 32.0 41.0 32-33 37.99683525722681 40.0 38.0 41.0 32.0 41.0 34-35 37.93633290095407 40.0 38.0 41.0 32.0 41.0 36-37 37.86050199769976 40.0 38.0 41.0 32.0 41.0 38-39 37.80347376545562 40.0 38.0 41.0 32.0 41.0 40-41 37.7737909815308 40.0 38.0 41.0 32.0 41.0 42-43 37.6110168860984 40.0 37.5 41.0 31.0 41.0 44-45 37.4647852192629 40.0 37.0 41.0 31.0 41.0 46-47 37.340082498680005 40.0 37.0 41.0 31.0 41.0 48-49 37.23325296069233 40.0 37.0 41.0 30.0 41.0 50-51 37.10245864532918 40.0 36.0 41.0 30.0 41.0 52-53 37.01569866153668 40.0 36.0 41.0 30.0 41.0 54-55 36.906241119715865 39.0 36.0 41.0 30.0 41.0 56-57 36.704165071763896 39.0 35.0 41.0 30.0 41.0 58-59 36.52685969397588 39.0 35.0 41.0 29.5 41.0 60-61 36.286071546068115 39.0 35.0 40.5 29.0 41.0 62-63 36.0716931950915 38.0 35.0 40.0 29.0 41.0 64-65 35.80397688817811 38.0 35.0 40.0 29.0 41.0 66-67 35.545072624476376 37.0 34.0 40.0 28.5 41.0 68-69 35.26497770572453 37.0 34.0 39.5 28.0 41.0 70-71 34.930969209337604 36.0 34.0 39.0 28.0 41.0 72-73 34.572709861563936 36.0 34.0 39.0 28.0 40.5 74-75 34.24761145568979 35.0 34.0 38.5 28.0 40.0 76-77 33.94866254516899 35.0 34.0 37.0 27.0 39.0 78-79 33.61161391012189 35.0 33.0 37.0 27.0 39.0 80-81 33.339740845855744 35.0 33.0 36.0 26.0 39.0 82-83 33.07973307378626 35.0 33.0 36.0 26.0 37.0 84-85 32.88592952875442 35.0 33.0 35.5 26.0 37.0 86-87 32.71711314232748 35.0 33.0 35.0 26.0 37.0 88-89 32.54012162795722 35.0 33.0 35.0 26.0 36.0 90-91 32.39679417820898 35.0 33.0 35.0 26.0 36.0 92-93 32.24338119087467 35.0 33.0 35.0 25.0 36.0 94-95 32.150049236553286 35.0 32.5 35.0 25.0 35.5 96-97 32.08025165996882 35.0 32.0 35.0 25.0 35.0 98-99 32.02320874864303 35.0 32.0 35.0 25.0 35.0 100 30.818776764111863 34.0 30.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2249801074442601 1101 2 0.2538250171721721 1101 3 0.25813993478969977 1101 4 0.29470011640687943 1101 5 0.1838728416776121 1101 6 0.20778936469363174 1101 7 0.3208411178834112 1101 8 0.32684959906565325 1101 9 0.42454664276345966 1101 10-11 0.44968728813634584 1101 12-13 0.4270343641548777 1101 14-15 0.5717747722886273 1101 16-17 0.49072322407549507 1101 18-19 0.45669227208246355 1101 20-21 0.53669278393307 1101 22-23 0.5114866952201069 1101 24-25 0.5120980531441646 1101 26-27 0.47483501343224077 1101 28-29 0.504952790039404 1101 30-31 0.5474173636451027 1101 32-33 0.5557723637715171 1101 34-35 0.4872771801004845 1101 36-37 0.5072792553202916 1101 38-39 0.5280125486997918 1101 40-41 0.5413484259422177 1101 42-43 0.5191979639769855 1101 44-45 0.4676380807046314 1101 46-47 0.48378008442994513 1101 48-49 0.5023873342497183 1101 50-51 0.46804780347515873 1101 52-53 0.44382474780414327 1101 54-55 0.41883373931965906 1101 56-57 0.3740034975729145 1101 58-59 0.4454503219811201 1101 60-61 0.5018056991491449 1101 62-63 0.4840736814699582 1101 64-65 0.48352413277687845 1101 66-67 0.47056017410429263 1101 68-69 0.43849787963556963 1101 70-71 0.40324328092172834 1101 72-73 0.4461018357616737 1101 74-75 0.43210715796814725 1101 76-77 0.360195247235346 1101 78-79 0.41410054613876923 1101 80-81 0.3773581797747667 1101 82-83 0.4434902061309103 1101 84-85 0.3560595951744574 1101 86-87 0.34421463662300766 1101 88-89 0.3632068829867663 1101 90-91 0.381595079097103 1101 92-93 0.3699663960113426 1101 94-95 0.30752223848757154 1101 96-97 0.3692767473917087 1101 98-99 0.39584895245947394 1101 100 0.4066034759250172 1102 1 0.18128584143605053 1102 2 0.2389992532445504 1102 3 0.20881970624186152 1102 4 0.2901037851681849 1102 5 0.27691019327203037 1102 6 0.33801846799072166 1102 7 0.30762308046136155 1102 8 0.34608730368546503 1102 9 0.40696256659203556 1102 10-11 0.4710037151516602 1102 12-13 0.442568649305386 1102 14-15 0.5473481598826879 1102 16-17 0.5434926736460781 1102 18-19 0.46662432844002666 1102 20-21 0.499245941420007 1102 22-23 0.4884344796623239 1102 24-25 0.5456139077566249 1102 26-27 0.46426936310221834 1102 28-29 0.48333216089222475 1102 30-31 0.5019132272413103 1102 32-33 0.44686826670687907 1102 34-35 0.4477726248805993 1102 36-37 0.48463071697779725 1102 38-39 0.4591006288920525 1102 40-41 0.4162729585768403 1102 42-43 0.4583759171673947 1102 44-45 0.40998311139348687 1102 46-47 0.40440530834282384 1102 48-49 0.390554389517348 1102 50-51 0.41370270553024113 1102 52-53 0.37136812404420994 1102 54-55 0.31808877521053347 1102 56-57 0.35534188425301494 1102 58-59 0.32436325125587473 1102 60-61 0.2866062445619164 1102 62-63 0.279413575963126 1102 64-65 0.3834777799555127 1102 66-67 0.370736740029038 1102 68-69 0.3233494588659269 1102 70-71 0.31303970110874957 1102 72-73 0.3356198985518972 1102 74-75 0.3417576375307334 1102 76-77 0.26038832971863 1102 78-79 0.32845470674283916 1102 80-81 0.2260060262691468 1102 82-83 0.29510819951715206 1102 84-85 0.297628144090055 1102 86-87 0.26703718904281004 1102 88-89 0.2118369291711346 1102 90-91 0.3022270818439594 1102 92-93 0.2542141736966457 1102 94-95 0.3009403820789984 1102 96-97 0.27537943886747485 1102 98-99 0.2813612096871907 1102 100 0.2757473696233852 1103 1 0.19269486197748975 1103 2 0.1994124237033823 1103 3 0.20762286486236903 1103 4 0.20825024212742704 1103 5 0.20245885398050945 1103 6 0.19727902816452314 1103 7 0.272817111865443 1103 8 0.22340455771504253 1103 9 0.4036582436530125 1103 10-11 0.34868197296690795 1103 12-13 0.33830360097640977 1103 14-15 0.41938695315435837 1103 16-17 0.430170007811995 1103 18-19 0.40087935972702127 1103 20-21 0.3267056455920425 1103 22-23 0.4155279763905284 1103 24-25 0.46004950598217675 1103 26-27 0.3985536649664354 1103 28-29 0.3780036149244026 1103 30-31 0.37610178179516396 1103 32-33 0.4034014595414419 1103 34-35 0.347696521379838 1103 36-37 0.3735846515938306 1103 38-39 0.42399870223761127 1103 40-41 0.37574296599205326 1103 42-43 0.39266021903160464 1103 44-45 0.3397337260756075 1103 46-47 0.38713892007186956 1103 48-49 0.2546348380726755 1103 50-51 0.32852857811867153 1103 52-53 0.2871924485443387 1103 54-55 0.24944601935316513 1103 56-57 0.30219302060186237 1103 58-59 0.283403956871922 1103 60-61 0.33835012050671054 1103 62-63 0.3748681805501448 1103 64-65 0.33044599861322865 1103 66-67 0.37806414119204845 1103 68-69 0.3277107748710719 1103 70-71 0.33106387210094823 1103 72-73 0.3062582573181061 1103 74-75 0.33694425372191006 1103 76-77 0.3195214002672273 1103 78-79 0.28972973308636085 1103 80-81 0.24394238552970648 1103 82-83 0.29497160349014706 1103 84-85 0.24646967968958222 1103 86-87 0.24698359136363734 1103 88-89 0.1991660215230553 1103 90-91 0.24198823393387414 1103 92-93 0.23787793932388723 1103 94-95 0.33384701544225237 1103 96-97 0.26752191502821177 1103 98-99 0.20824330780217082 1103 100 0.21626955672091341 1104 1 0.11901819338161346 1104 2 0.09150300806893696 1104 3 0.05698438407725703 1104 4 0.09697839632627137 1104 5 0.029184635450597796 1104 6 0.14114655239423257 1104 7 0.16207937902697722 1104 8 0.19155621541075618 1104 9 0.2091420622444602 1104 10-11 0.20879763610806634 1104 12-13 0.22212530977044764 1104 14-15 0.3068110828215893 1104 16-17 0.28277629703714524 1104 18-19 0.27022013339122 1104 20-21 0.26001204538454203 1104 22-23 0.3045900355957514 1104 24-25 0.37770696429264916 1104 26-27 0.3237470143372221 1104 28-29 0.39019580398373677 1104 30-31 0.3534623161759711 1104 32-33 0.32406251548986376 1104 34-35 0.29298543488113893 1104 36-37 0.3228101301213755 1104 38-39 0.29946575214665216 1104 40-41 0.2821408087428878 1104 42-43 0.3152836983999876 1104 44-45 0.21182856893979363 1104 46-47 0.28238967280004346 1104 48-49 0.2532239993890073 1104 50-51 0.27310759179513155 1104 52-53 0.2887603221785682 1104 54-55 0.24291994616354629 1104 56-57 0.35374219437407817 1104 58-59 0.3338679253059098 1104 60-61 0.3492579794652073 1104 62-63 0.20630150774163525 1104 64-65 0.3082671997973847 1104 66-67 0.2355877358938372 1104 68-69 0.1836614457238781 1104 70-71 0.20100148548988273 1104 72-73 0.16267472959371787 1104 74-75 0.18008690473240563 1104 76-77 0.19030507233491534 1104 78-79 0.14647296410346655 1104 80-81 0.090073567938596 1104 82-83 0.1434155732425353 1104 84-85 0.09274842965479735 1104 86-87 0.1311733468665608 1104 88-89 0.17180483019231474 1104 90-91 0.1693246468572127 1104 92-93 0.14245712799942822 1104 94-95 0.11958974152228308 1104 96-97 0.14924325498213875 1104 98-99 0.17948274275994436 1104 100 0.2083393972238774 1105 1 0.12285823346183733 1105 2 -0.005107095359726088 1105 3 0.025871213275951987 1105 4 0.09762012721802193 1105 5 0.08075272857337268 1105 6 0.10872531265215457 1105 7 0.06540444883931684 1105 8 0.10708840704221956 1105 9 0.08400615902173314 1105 10-11 0.18677057034194178 1105 12-13 0.23196587673015756 1105 14-15 0.23296056052454617 1105 16-17 0.1905323703658297 1105 18-19 0.22062782482687737 1105 20-21 0.22190757873710965 1105 22-23 0.15539828617300344 1105 24-25 0.25113437854042076 1105 26-27 0.17316085483405885 1105 28-29 0.23000493887901285 1105 30-31 0.23688604367193733 1105 32-33 0.223589979737973 1105 34-35 0.21451724851858245 1105 36-37 0.23893751243385708 1105 38-39 0.2494265306069039 1105 40-41 0.24129715492198756 1105 42-43 0.2649905315374568 1105 44-45 0.2564109575244089 1105 46-47 0.16739853610361166 1105 48-49 0.20195232662173623 1105 50-51 0.2421420639374361 1105 52-53 0.25475679630516623 1105 54-55 0.15876381945002294 1105 56-57 0.1476822375632807 1105 58-59 0.11454599375696972 1105 60-61 0.19888193969463686 1105 62-63 0.1680387017353624 1105 64-65 0.2534922079405675 1105 66-67 0.20278933947015076 1105 68-69 0.09194561403802481 1105 70-71 0.10695176893003122 1105 72-73 0.15633691448940823 1105 74-75 0.11011525104311204 1105 76-77 0.1370491605194033 1105 78-79 0.17226492493168166 1105 80-81 0.09207115049473202 1105 82-83 0.14435277909955602 1105 84-85 0.13083247587314162 1105 86-87 0.0999492142627787 1105 88-89 0.07082694284290625 1105 90-91 0.06003933189276012 1105 92-93 0.14501346319580222 1105 94-95 0.1748089452395405 1105 96-97 0.13112559392835976 1105 98-99 0.13469833778761142 1105 100 0.21363466298779343 1106 1 0.05623652318708494 1106 2 0.053216356207883564 1106 3 0.00681824043459045 1106 4 0.1270607090151188 1106 5 0.03739003172634625 1106 6 0.14311958330433328 1106 7 0.17891005310622887 1106 8 0.21627835244071036 1106 9 0.21342880210937665 1106 10-11 0.2498933424309655 1106 12-13 0.26805052577304167 1106 14-15 0.2987179113008622 1106 16-17 0.29216489609613916 1106 18-19 0.284645262645256 1106 20-21 0.33219432597708476 1106 22-23 0.32312078323619886 1106 24-25 0.30797852494350053 1106 26-27 0.2930290652872358 1106 28-29 0.24036559463363005 1106 30-31 0.21959793806800576 1106 32-33 0.2486442955967334 1106 34-35 0.18539779670283707 1106 36-37 0.20042396728963752 1106 38-39 0.1836398021309975 1106 40-41 0.1638101708033588 1106 42-43 0.1863025308317674 1106 44-45 0.15308562890491828 1106 46-47 0.16101123356320812 1106 48-49 0.17167691778100647 1106 50-51 0.16953167478092723 1106 52-53 0.11269236680578132 1106 54-55 0.07430672462594856 1106 56-57 0.055460621895548456 1106 58-59 0.08130553934921636 1106 60-61 0.1183806720559275 1106 62-63 0.08364558529920174 1106 64-65 0.11462358983634857 1106 66-67 0.04157971591352094 1106 68-69 0.016152593472732235 1106 70-71 0.023076463465784514 1106 72-73 0.018461360643037494 1106 74-75 0.0879660259992221 1106 76-77 0.0943592362063228 1106 78-79 0.08945663092874412 1106 80-81 0.07515619841920085 1106 82-83 0.033894201978391436 1106 84-85 0.08968823381843549 1106 86-87 0.04355567637657032 1106 88-89 -0.0258811040804261 1106 90-91 0.06829308557738756 1106 92-93 0.08606899181965133 1106 94-95 0.16689562725019158 1106 96-97 0.11000510422093157 1106 98-99 0.04464072886669612 1106 100 0.14619896603926819 1107 1 0.03701576823769415 1107 2 0.05715912184760796 1107 3 0.014310696583777371 1107 4 0.04409564343079353 1107 5 0.03599054348855901 1107 6 0.08286768270295397 1107 7 0.09940645153562855 1107 8 0.11669539556672248 1107 9 0.1245424886844475 1107 10-11 0.16304582560633918 1107 12-13 0.16314145549463177 1107 14-15 0.11699171804357178 1107 16-17 0.11587707141323023 1107 18-19 0.13959386494703097 1107 20-21 0.20428350391123473 1107 22-23 0.2137835195560811 1107 24-25 0.2211315542251171 1107 26-27 0.2166405750221827 1107 28-29 0.13279000898020143 1107 30-31 0.2091068125976676 1107 32-33 0.2106700705973239 1107 34-35 0.1995310866712927 1107 36-37 0.20185834564781402 1107 38-39 0.1432754521885542 1107 40-41 0.17483387120704919 1107 42-43 0.20618091904169233 1107 44-45 0.20528064222688158 1107 46-47 0.19922422834304854 1107 48-49 0.2186115324818232 1107 50-51 0.2054112789874054 1107 52-53 0.18206199638192544 1107 54-55 0.16514075626734837 1107 56-57 0.1791279558231338 1107 58-59 0.10951860087536147 1107 60-61 0.1810083774813691 1107 62-63 0.2249801233024371 1107 64-65 0.20352064335050812 1107 66-67 0.1678272804314176 1107 68-69 0.21230333335565632 1107 70-71 0.1743392127708674 1107 72-73 0.1619949470864981 1107 74-75 0.1598133395509791 1107 76-77 0.2094473019041132 1107 78-79 0.22175669081839544 1107 80-81 0.22410600579539874 1107 82-83 0.1853068271049807 1107 84-85 0.14420802137097155 1107 86-87 0.1486415637172911 1107 88-89 0.15467520827048986 1107 90-91 0.13981570863484905 1107 92-93 0.10913020850792776 1107 94-95 0.18991552190022531 1107 96-97 0.2315917734037356 1107 98-99 0.16448501577635 1107 100 0.3258869694809512 1108 1 0.010293215036433878 1108 2 -0.01293462444073512 1108 3 0.00992443760581807 1108 4 -0.006105462352323343 1108 5 -0.028407723972641463 1108 6 0.022903605075583755 1108 7 -0.014989789523312425 1108 8 -0.06726344012110275 1108 9 -0.03247658222065297 1108 10-11 -0.05689577652585598 1108 12-13 -0.02285041218996753 1108 14-15 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-0.1974000168479435 2214 82-83 -0.16663844958160468 2214 84-85 -0.19907564564719138 2214 86-87 -0.12738917351416035 2214 88-89 -0.22915163888254142 2214 90-91 -0.2560762897368818 2214 92-93 -0.18346691190684794 2214 94-95 -0.17776158518211815 2214 96-97 -0.1783692453481862 2214 98-99 -0.24655874609243256 2214 100 -0.14267845409518998 2215 1 -0.32957092011551836 2215 2 -0.3347711021659734 2215 3 -0.46031750566301 2215 4 -0.3875920108775688 2215 5 -0.32009901089262627 2215 6 -0.26513225190872447 2215 7 -0.3822003808799508 2215 8 -0.3445240215138483 2215 9 -0.48986983243411686 2215 10-11 -0.5113009096236567 2215 12-13 -0.489828586249871 2215 14-15 -0.5644387669550994 2215 16-17 -0.667611974425121 2215 18-19 -0.7117631394844253 2215 20-21 -0.5946869883832946 2215 22-23 -0.5020013969133927 2215 24-25 -0.5051613797009438 2215 26-27 -0.5804625298654997 2215 28-29 -0.6000028304037741 2215 30-31 -0.6111446334852388 2215 32-33 -0.5969645583252472 2215 34-35 -0.6113831513829453 2215 36-37 -0.5697180226269225 2215 38-39 -0.6889572155563215 2215 40-41 -0.6854206834438159 2215 42-43 -0.693222220174853 2215 44-45 -0.6040648966939912 2215 46-47 -0.6421088944888353 2215 48-49 -0.5535940935191661 2215 50-51 -0.5896520161243544 2215 52-53 -0.5797112458238658 2215 54-55 -0.6452857443269622 2215 56-57 -0.6036778606956545 2215 58-59 -0.540165764959724 2215 60-61 -0.5104443544087474 2215 62-63 -0.5400237392418674 2215 64-65 -0.5403083291148008 2215 66-67 -0.5174721128645814 2215 68-69 -0.5471620593925834 2215 70-71 -0.42390149161875 2215 72-73 -0.3895549441617021 2215 74-75 -0.4086055768718211 2215 76-77 -0.505468937662279 2215 78-79 -0.46557203963143934 2215 80-81 -0.5305144092963374 2215 82-83 -0.43745157063978723 2215 84-85 -0.43414816334614414 2215 86-87 -0.41218628394645407 2215 88-89 -0.4166085734755143 2215 90-91 -0.4534464965374596 2215 92-93 -0.47357684366662056 2215 94-95 -0.47715940219293174 2215 96-97 -0.48377682574144387 2215 98-99 -0.5042853191840848 2215 100 -0.4580011899085079 2216 1 -0.2941912065135277 2216 2 -0.25805023850353237 2216 3 -0.3173262723442285 2216 4 -0.32488926881879365 2216 5 -0.2618018627194303 2216 6 -0.32870991956330187 2216 7 -0.33485461967414665 2216 8 -0.29225878775843483 2216 9 -0.3762737905651008 2216 10-11 -0.5075134129907823 2216 12-13 -0.42783761608579596 2216 14-15 -0.5718765224307631 2216 16-17 -0.630223971949178 2216 18-19 -0.5884222711138278 2216 20-21 -0.6265422490746104 2216 22-23 -0.6596387271017434 2216 24-25 -0.6178219515224797 2216 26-27 -0.7201881364171285 2216 28-29 -0.7572437636917826 2216 30-31 -0.7300770777529877 2216 32-33 -0.7129232211707901 2216 34-35 -0.7266922516191343 2216 36-37 -0.7295978837886778 2216 38-39 -0.6855512988040005 2216 40-41 -0.720063541656927 2216 42-43 -0.6630219706495737 2216 44-45 -0.5791211930450402 2216 46-47 -0.6144691036762993 2216 48-49 -0.48294743105009275 2216 50-51 -0.5605695359350449 2216 52-53 -0.6191411113197347 2216 54-55 -0.6402249828919722 2216 56-57 -0.5624423165852761 2216 58-59 -0.5357743221604778 2216 60-61 -0.5788713496203499 2216 62-63 -0.5895532072975342 2216 64-65 -0.581606027016079 2216 66-67 -0.5803242459993285 2216 68-69 -0.6286134924758713 2216 70-71 -0.6476368256826106 2216 72-73 -0.588484589289088 2216 74-75 -0.5303376054638917 2216 76-77 -0.6291902205298427 2216 78-79 -0.5039330819016783 2216 80-81 -0.5433266363676594 2216 82-83 -0.5389886671853787 2216 84-85 -0.5314357164168797 2216 86-87 -0.6057997266571391 2216 88-89 -0.6066426792583819 2216 90-91 -0.5960729647959084 2216 92-93 -0.5541879242002885 2216 94-95 -0.5115675743509414 2216 96-97 -0.5273464666778835 2216 98-99 -0.5284083153208954 2216 100 -0.7079340138693269 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 1347.0 1 303.0 2 231.0 3 221.0 4 184.0 5 263.0 6 354.0 7 855.0 8 2324.0 9 3697.0 10 4401.0 11 5587.0 12 7427.0 13 8769.0 14 10750.0 15 11591.0 16 12248.0 17 13346.0 18 14706.0 19 16142.0 20 17742.0 21 19450.0 22 21961.0 23 24954.0 24 29869.0 25 35448.0 26 39474.0 27 40924.0 28 46028.0 29 56120.0 30 66769.0 31 78824.0 32 99610.0 33 134045.0 34 195976.0 35 299260.0 36 538973.0 37 1035120.0 38 1234770.0 39 331589.0 40 480.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.44362045461836 19.36169215242598 13.190307702212433 24.004379690743228 2 14.616384081462039 21.208444187702835 39.85931436834089 24.315857362494235 3 19.758371282669625 25.66584039388361 29.581574924370475 24.994213399076298 4 12.20859633249344 17.29682543883534 39.55372046151783 30.94085776715339 5 13.069467932873682 38.354678521283134 35.234475581254515 13.341377964588668 6 29.9837314708002 37.481587716935216 18.776524958723066 13.758155853541517 7 26.606440655764768 32.388927218293084 23.006113365518072 17.99851876042408 8 24.9937533448203 34.62948250779542 21.0352580507834 19.341506096600877 9 25.526210855729843 17.119059761549458 21.20369485758319 36.15103452513751 10-11 24.40359838738454 26.192609784042258 28.646374816805587 20.757417011767615 12-13 25.85937803977317 23.880945789312513 27.962207269318114 22.297468901596204 14-15 22.98362046266945 25.410834106721776 26.272345468467055 25.333199962141723 16-17 22.233402715188884 28.19093456024946 27.095537817036224 22.480124907525436 18-19 22.043934953992338 28.8515528041556 27.517867197528762 21.586645044323298 20-21 23.240275081940375 27.396847075656506 26.72436973470717 22.638508107695948 22-23 22.68585026481435 27.442662992499123 26.455251023323466 23.416235719363062 24-25 23.24420187630221 27.559394182700608 26.378967742224873 22.817436198772306 26-27 23.116672452722543 26.948958913620913 27.221430564189784 22.71293806946676 28-29 22.39003675123233 28.281717282409424 26.833434784617506 22.494811181740744 30-31 23.005339281568883 27.345835394020142 26.726384529204555 22.922440795206423 32-33 22.766518118339 27.66030271829197 26.793730308804598 22.779448854564432 34-35 23.21636471321703 27.546761073872624 26.893963820764327 22.34291039214602 36-37 23.185737230404893 27.02985810645084 27.25353399264485 22.53087067049942 38-39 22.976606138202644 27.567932460301048 27.09620387521539 22.359257526280913 40-41 22.874723494128048 27.572699006844292 27.03876398118212 22.513813517845534 42-43 22.82726520162074 27.440983354850417 26.36399638364944 23.367755059879403 44-45 23.103157919292137 27.072763047041754 26.709975120293784 23.11410391337233 46-47 23.30210139862963 27.519915377030678 26.8757104624028 22.30227276193689 48-49 23.130361148497084 27.24744279946164 27.10391431135038 22.51828174069089 50-51 22.10030583275976 27.476340831987578 27.582371229536516 22.840982105716144 52-53 22.540827501367236 27.88797286257078 26.415766878679705 23.155432757382286 54-55 22.393808234117316 27.21892601873361 26.796102545767187 23.59116320138189 56-57 23.125397464526817 26.737857914352915 26.81768168965745 23.319062931462813 58-59 23.552231081761185 26.805912313109108 26.82363413280799 22.818222472321715 60-61 22.92784130025683 27.28725228682046 27.45370109696944 22.331205315953266 62-63 22.319978563395292 27.23615293702908 27.27775421730315 23.16611428227248 64-65 22.02463974548404 27.88407472749781 26.93367781463895 23.157607712379196 66-67 22.91654491803742 26.903409189693093 26.51067662102154 23.669369271247945 68-69 22.613643169969045 26.967815804710316 26.810808240126683 23.607732785193956 70-71 23.04780804322996 26.858370089438395 26.75284362307983 23.34097824425182 72-73 23.05215646524568 26.33286669487958 27.60111306786948 23.013863772005262 74-75 22.617006825994075 27.629645374717406 26.786387495043567 22.96696030424495 76-77 22.611306758205355 26.79205902486577 26.916685669304304 23.679948547624573 78-79 22.832667006401607 26.87069682941601 27.107404512925875 23.189231651256513 80-81 23.039144096821456 26.74169122580793 27.068032242411675 23.15113243495894 82-83 22.81802360439558 26.40576161276699 27.661417194764027 23.1147975880734 84-85 22.78245218731857 26.960963782336876 27.042678084459727 23.21390594588483 86-87 22.498985193300065 27.11535461142858 27.058296621016158 23.32736357425519 88-89 22.652999442882816 26.747928532419635 26.680473272563777 23.918598752133775 90-91 23.057536894250706 26.303988683142226 26.77793594090504 23.860538481702022 92-93 23.245229157890346 26.69717798357925 27.016467856343336 23.041125002187073 94-95 22.69631834890188 26.535853192454866 27.338798082649568 23.429030375993683 96-97 22.35595061958797 26.516736636307108 27.781618456552575 23.345694287552345 98-99 22.75553903417447 26.87903944869836 26.817514254271458 23.54790726285571 100 22.805394936692366 25.87331194810394 26.690410726252367 24.630882388951328 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1485.0 1 918.0 2 340.5 3 345.0 4 333.0 5 311.0 6 296.5 7 300.0 8 306.0 9 302.5 10 307.0 11 307.0 12 337.0 13 362.5 14 389.5 15 435.5 16 499.0 17 625.5 18 838.5 19 1066.0 20 1488.5 21 2242.5 22 3399.5 23 5118.5 24 7321.5 25 10580.0 26 15537.5 27 21238.0 28 27711.5 29 35585.0 30 44707.0 31 54324.0 32 64320.0 33 73891.5 34 83433.0 35 92762.0 36 102606.0 37 115250.5 38 123853.0 39 127546.0 40 131411.5 41 135739.0 42 140100.0 43 146581.5 44 157359.0 45 167718.0 46 176362.5 47 201036.0 48 245747.0 49 247756.5 50 219183.5 51 196956.5 52 171127.0 53 157570.0 54 146864.5 55 131510.5 56 117464.0 57 103156.0 58 88864.5 59 78128.5 60 64946.5 61 50929.5 62 39994.5 63 31625.0 64 23816.5 65 17030.0 66 12557.0 67 9245.0 68 6829.5 69 5055.5 70 3922.0 71 2974.0 72 2207.5 73 1920.5 74 1611.0 75 1221.0 76 899.5 77 627.5 78 440.5 79 325.5 80 240.0 81 171.0 82 115.5 83 82.0 84 70.0 85 51.5 86 42.0 87 37.0 88 31.5 89 32.5 90 31.5 91 26.0 92 20.5 93 15.0 94 11.5 95 10.0 96 7.5 97 7.5 98 7.0 99 7.0 100 14.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09598550647986209 2 0.06337777546697408 3 0.07969284637926444 4 0.0812616031977539 5 0.0822925005356184 6 0.08585581959475874 7 0.08300964650978501 8 0.08395090060087868 9 0.07122155955942137 10-11 0.07647689490136104 12-13 0.06769185671782009 14-15 0.07615193813181681 16-17 0.07784395441461615 18-19 0.07782154360292345 20-21 0.06940628381231215 22-23 0.0729135758422207 24-25 0.07534514891087937 26-27 0.0702803054683277 28-29 0.07857230579462911 30-31 0.08218044647715488 32-33 0.08432067899380834 34-35 0.08642729529292276 36-37 0.0873013169489383 38-39 0.08861234943296165 40-41 0.08556447904275355 42-43 0.090685349514537 44-45 0.08695394936770136 46-47 0.08444393845811822 48-49 0.0926014739142634 50-51 0.08911659269604753 52-53 0.09479773346014865 54-55 0.08714444126708937 56-57 0.09383406855736227 58-59 0.09749823626911978 60-61 0.09647854433710164 62-63 0.09718448490542189 64-65 0.0989549390291457 66-67 0.10126325263349449 68-69 0.09827140927251816 70-71 0.10045646341255705 72-73 0.09776716600943226 74-75 0.10173387967904132 76-77 0.09899976065253112 78-79 0.1061263987708118 80-81 0.10140892290949707 82-83 0.10531960954987436 84-85 0.1014761553445752 86-87 0.09830502549005724 88-89 0.09776716600943226 90-91 0.09765511195096872 92-93 0.09219807930379469 94-95 0.09199638199856033 96-97 0.08982253326436779 98-99 0.10328022568583807 100 0.2414316743655275 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4462132.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.336195244458494 #Duplication Level Percentage of deduplicated Percentage of total 1 74.1240873052053 45.4648949126936 2 14.400183504316894 17.665049339536232 3 5.268690586977461 9.694843035764704 4 2.4052724075447096 5.901210320210843 5 1.2901639113364611 3.9566872781543716 6 0.7690558298611042 2.8302575120549847 7 0.4722014719100771 2.027412917305802 8 0.29819003124677984 1.4631873581202934 9 0.22433080902306096 1.2383638471427219 >10 0.7303194234904662 6.863728683433395 >50 0.013501305851077386 0.545904686890225 >100 0.0033214894819075624 0.3080187209056746 >500 2.2730791826961773E-4 0.10149923678740658 >1k 2.841348978182355E-4 0.38351514502496825 >5k 5.6826979563647096E-5 0.17790250842565103 >10k+ 1.1365395912729419E-4 1.3775244975490113 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 29341 0.6575556258757024 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 10180 0.22814206303175252 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5104 0.11438478287957415 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 5.602702923176634E-5 14-15 0.0 0.0 0.0 1.1205405846353268E-5 1.0084865261717941E-4 16-17 0.0 0.0 0.0 2.2410811692706535E-5 1.2325946430988594E-4 18-19 0.0 0.0 0.0 5.602702923176634E-5 1.344648701562392E-4 20-21 2.2410811692706535E-5 0.0 0.0 1.0084865261717941E-4 1.7928649354165228E-4 22-23 2.2410811692706535E-5 0.0 0.0 3.137513636978915E-4 1.7928649354165228E-4 24-25 2.2410811692706535E-5 0.0 0.0 7.059405683202558E-4 1.7928649354165228E-4 26-27 2.2410811692706535E-5 0.0 0.0 0.001389470324947805 1.9049189938800555E-4 28-29 2.2410811692706535E-5 0.0 0.0 0.0035857298708330456 2.0169730523435882E-4 30-31 2.2410811692706535E-5 0.0 0.0 0.011272638281431387 2.0169730523435882E-4 32-33 2.2410811692706535E-5 0.0 0.0 0.03140875258732821 2.0169730523435882E-4 34-35 2.2410811692706535E-5 0.0 0.0 0.06160732134325027 2.0169730523435882E-4 36-37 4.482162338541307E-5 0.0 0.0 0.11087749084966558 2.0169730523435882E-4 38-39 6.72324350781196E-5 0.0 0.0 0.1878922452316516 2.2410811692706535E-4 40-41 6.72324350781196E-5 0.0 0.0 0.27643736222953513 2.2410811692706535E-4 42-43 6.72324350781196E-5 0.0 0.0 0.36502730085080404 2.2410811692706535E-4 44-45 6.72324350781196E-5 0.0 0.0 0.47610648900570396 2.2410811692706535E-4 46-47 6.72324350781196E-5 0.0 0.0 0.6138993646983102 2.2410811692706535E-4 48-49 6.72324350781196E-5 0.0 0.0 0.7553115864792883 2.2410811692706535E-4 50-51 6.72324350781196E-5 0.0 0.0 0.9064052789115158 2.2410811692706535E-4 52-53 6.72324350781196E-5 0.0 0.0 1.0595383552077795 2.2410811692706535E-4 54-55 6.72324350781196E-5 0.0 0.0 1.231373253861607 2.3531352277341862E-4 56-57 6.72324350781196E-5 0.0 0.0 1.4447914136112514 2.465189286197719E-4 58-59 7.843784092447287E-5 0.0 0.0 1.6758132659455165 2.465189286197719E-4 60-61 8.964324677082614E-5 0.0 0.0 1.9200574971784787 2.465189286197719E-4 62-63 8.964324677082614E-5 0.0 0.0 2.1738711450042265 2.689297403124784E-4 64-65 8.964324677082614E-5 0.0 0.0 2.4375455499747654 2.689297403124784E-4 66-67 8.964324677082614E-5 0.0 0.0 2.7376487293517986 2.689297403124784E-4 68-69 8.964324677082614E-5 0.0 0.0 3.075895110229818 2.689297403124784E-4 70-71 8.964324677082614E-5 0.0 0.0 3.453125098047301 2.689297403124784E-4 72-73 8.964324677082614E-5 0.0 0.0 3.8232396531523496 2.801351461588317E-4 74-75 8.964324677082614E-5 0.0 0.0 4.195953862413752 2.9134055200518496E-4 76-77 8.964324677082614E-5 0.0 0.0 4.601892100009591 2.9134055200518496E-4 78-79 8.964324677082614E-5 0.0 0.0 5.0653925074381485 2.9134055200518496E-4 80-81 8.964324677082614E-5 0.0 0.0 5.569478894842197 2.9134055200518496E-4 82-83 8.964324677082614E-5 0.0 0.0 6.0782939635134055 2.9134055200518496E-4 84-85 8.964324677082614E-5 0.0 0.0 6.584285269911334 2.9134055200518496E-4 86-87 8.964324677082614E-5 0.0 0.0 7.109942063569612 3.025459578515382E-4 88 8.964324677082614E-5 0.0 0.0 7.529539690892157 3.137513636978915E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATGGGG 7550 0.0 19.86122 4 GTACCCG 730 0.0 18.036545 1 TATTCGC 270 5.467573E-7 17.403152 9 TCTAACG 630 0.0 17.161657 2 TAGTACT 2445 0.0 16.918661 4 TACCTGG 4435 0.0 16.640944 2 ACATGGG 23505 0.0 16.459307 3 GTGATCG 800 0.0 16.446716 8 TGATCGC 745 0.0 16.398674 9 GTACATG 26255 0.0 16.388048 1 GTACTAG 1780 0.0 16.379093 1 CTAACGC 665 0.0 16.258413 3 CGTTAAC 435 9.458745E-11 16.21512 1 TACATGG 25940 0.0 16.164669 2 CTAGTAC 2345 0.0 15.635929 3 ATGGGGG 5100 0.0 15.484125 5 GTATAAG 1970 0.0 15.276784 1 TACGGGC 1065 0.0 15.006917 4 CTGTGCG 2195 0.0 14.984946 9 TGCGCGA 900 0.0 14.644108 94 >>END_MODULE