FastQCFastQC Report
Mon 16 Jan 2017
ERR1146637_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146637_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4462132
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA98220.2201189924457636No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT79500.17816595295701695No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79200.17749362860623577No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70530.15806345486865922No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC64070.14358607051517078No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA50380.11290566930785553No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT104250.021.07567212-13
CTAGTAC25150.020.19583
GTACTAG18200.020.1577631
GTACTTT116650.018.75494214-15
CGTTAAC5100.018.4450131
AGTACTT112300.018.28847912-13
CTGTGCG22450.017.3754169
CATGGGG75100.017.2213844
GTATAAG22050.016.8514631
TAGTACT27750.016.269854
TGATCGC9550.016.2399279
TAGGCAT25050.016.1359085
ACTTTTT143500.015.55696616-17
ACTGTGC44000.015.4877678
TACGGGC10050.015.4426984
CGGGCGT10500.015.2190466
TACCTGG42950.015.11112
GTGATCG10600.015.0746268
CCTAGTA28750.015.049782
GGCGTCG10700.014.9337418
Produced by FastQC (version 0.11.5)