##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146637_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4462132
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31927921451001	33.0	31.0	34.0	30.0	34.0
2	32.40677102335834	34.0	31.0	34.0	30.0	34.0
3	32.519155193078106	34.0	31.0	34.0	30.0	34.0
4	35.99106099954013	37.0	35.0	37.0	35.0	37.0
5	36.031001548138875	37.0	35.0	37.0	35.0	37.0
6	36.10326610687447	37.0	35.0	37.0	35.0	37.0
7	36.07487003970299	37.0	35.0	37.0	35.0	37.0
8	36.084197643637616	37.0	36.0	37.0	35.0	37.0
9	37.872529544173055	39.0	38.0	39.0	35.0	39.0
10-11	37.81876342967891	39.0	38.0	39.0	35.0	39.0
12-13	37.79440097244994	39.0	38.0	39.0	35.0	39.0
14-15	39.14255282004208	40.0	39.0	41.0	36.0	41.0
16-17	39.09741352340092	40.0	39.0	41.0	36.0	41.0
18-19	39.18715470541885	40.0	39.0	41.0	36.0	41.0
20-21	39.242717158524215	40.0	39.0	41.0	36.0	41.0
22-23	39.21084338159427	40.0	39.0	41.0	36.0	41.0
24-25	39.20567331939082	41.0	39.0	41.0	36.0	41.0
26-27	39.1255295674803	40.0	39.0	41.0	36.0	41.0
28-29	39.07739551407265	40.0	39.0	41.0	35.0	41.0
30-31	38.98042281133772	40.0	39.0	41.0	35.0	41.0
32-33	38.89857303638709	40.0	38.0	41.0	35.0	41.0
34-35	38.83996439370239	40.0	38.0	41.0	35.0	41.0
36-37	38.788663468494434	40.0	38.0	41.0	35.0	41.0
38-39	38.720653154142454	40.0	38.0	41.0	35.0	41.0
40-41	38.66397968504742	40.0	38.0	41.0	35.0	41.0
42-43	38.586734659575285	40.0	38.0	41.0	35.0	41.0
44-45	38.48075068151279	40.0	38.0	41.0	34.0	41.0
46-47	38.300448193823044	40.0	38.0	41.0	34.0	41.0
48-49	38.064015587167745	40.0	38.0	41.0	33.0	41.0
50-51	37.967107315516444	40.0	37.5	41.0	33.0	41.0
52-53	37.90362913961308	40.0	37.0	41.0	33.0	41.0
54-55	37.76352660566742	40.0	37.0	41.0	33.0	41.0
56-57	37.60973386712899	40.0	36.5	41.0	33.0	41.0
58-59	37.44658506292508	39.5	36.0	41.0	33.0	41.0
60-61	37.22134912189958	39.0	36.0	41.0	32.0	41.0
62-63	37.001464770652234	39.0	35.0	41.0	32.0	41.0
64-65	36.74107467013526	39.0	35.0	40.5	32.0	41.0
66-67	36.458551651990575	38.0	35.0	40.0	31.5	41.0
68-69	36.14464856709752	37.0	35.0	40.0	31.0	41.0
70-71	35.793822549400154	37.0	35.0	39.0	31.0	41.0
72-73	35.444930696805926	36.0	35.0	39.0	31.0	41.0
74-75	35.12301507440837	36.0	35.0	39.0	31.0	40.5
76-77	34.799939244289504	35.0	34.0	37.0	30.5	39.5
78-79	34.48959398780673	35.0	34.0	37.0	30.0	39.0
80-81	34.203996094243735	35.0	34.0	36.5	30.0	39.0
82-83	33.93990764952718	35.0	34.0	36.0	30.0	37.5
84-85	33.726585295997516	35.0	34.0	36.0	30.0	37.0
86-87	33.55675347569279	35.0	34.0	35.0	30.0	37.0
88-89	33.35695604701968	35.0	34.0	35.0	29.0	36.0
90-91	33.26186663684535	35.0	34.0	35.0	29.0	36.0
92-93	33.15010940958268	35.0	34.0	35.0	29.0	36.0
94-95	33.0700130565389	35.0	34.0	35.0	29.0	36.0
96-97	33.014675495928856	35.0	34.0	35.0	29.0	35.0
98-99	32.96736593628337	35.0	34.0	35.0	29.0	35.0
100	32.1977458309167	35.0	32.0	35.0	26.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2904199428485299
1101	2	0.28093683679278314
1101	3	0.3065271337737201
1101	4	0.21240508407642267
1101	5	0.2222207449866147
1101	6	0.20965975599646214
1101	7	0.2192399478146143
1101	8	0.22284790541577593
1101	9	0.2503453509730704
1101	10-11	0.2783230250270847
1101	12-13	0.29759796648895787
1101	14-15	0.4076663340739799
1101	16-17	0.4697868330605388
1101	18-19	0.4045987461238738
1101	20-21	0.36541427096133816
1101	22-23	0.3480282972755475
1101	24-25	0.3506706124737562
1101	26-27	0.3614635010194789
1101	28-29	0.40040500502900755
1101	30-31	0.4463137135502677
1101	32-33	0.3766461899989295
1101	34-35	0.404680502593628
1101	36-37	0.44101227290157397
1101	38-39	0.42095336524347715
1101	40-41	0.43218590438003446
1101	42-43	0.4398601923079539
1101	44-45	0.4819694526592997
1101	46-47	0.4920702614484185
1101	48-49	0.5617146252055463
1101	50-51	0.510366813355212
1101	52-53	0.4849394864433805
1101	54-55	0.4933505859229115
1101	56-57	0.4942326013462832
1101	58-59	0.4596165203939151
1101	60-61	0.4757620742038853
1101	62-63	0.40195789010117977
1101	64-65	0.3524244478059657
1101	66-67	0.32135494671291553
1101	68-69	0.32789119163778224
1101	70-71	0.3424817636148134
1101	72-73	0.3175197258210005
1101	74-75	0.3060008712110829
1101	76-77	0.32877579642778443
1101	78-79	0.2574779373645981
1101	80-81	0.2616310065712426
1101	82-83	0.2848449776398354
1101	84-85	0.2920383335804999
1101	86-87	0.2513883815928679
1101	88-89	0.3905612497169102
1101	90-91	0.36334968360884545
1101	92-93	0.37496137025954823
1101	94-95	0.3265779764928638
1101	96-97	0.3212184360382224
1101	98-99	0.3886692073894906
1101	100	0.35780727283876246
1102	1	0.3329989419394508
1102	2	0.31896005240485437
1102	3	0.338134410026953
1102	4	0.20058182697099625
1102	5	0.1842268051702547
1102	6	0.20247425459789525
1102	7	0.18552205890069473
1102	8	0.2054257071736174
1102	9	0.2947484043477573
1102	10-11	0.2919361604472712
1102	12-13	0.3038016641565946
1102	14-15	0.45520152185832785
1102	16-17	0.5922240815665845
1102	18-19	0.4818540955005943
1102	20-21	0.3892720562220333
1102	22-23	0.40679270965584635
1102	24-25	0.41550068792765416
1102	26-27	0.3640126243956985
1102	28-29	0.41697876879356244
1102	30-31	0.4259734229728167
1102	32-33	0.4585497373048284
1102	34-35	0.4651673806810024
1102	36-37	0.4330738825768705
1102	38-39	0.40572417458866994
1102	40-41	0.4532350606015001
1102	42-43	0.4554745084037606
1102	44-45	0.43112775944246096
1102	46-47	0.4828238536198697
1102	48-49	0.5184230188047536
1102	50-51	0.501777169647589
1102	52-53	0.4469943448383944
1102	54-55	0.413326701257148
1102	56-57	0.4408919136825702
1102	58-59	0.34919873944663493
1102	60-61	0.3637455843223165
1102	62-63	0.3953402138954871
1102	64-65	0.3748054335555082
1102	66-67	0.29418166975943905
1102	68-69	0.2924631109332765
1102	70-71	0.29170216013369554
1102	72-73	0.3054434757429618
1102	74-75	0.3186498309997958
1102	76-77	0.28597909187416093
1102	78-79	0.32110609267698464
1102	80-81	0.3228331064379617
1102	82-83	0.274000862829368
1102	84-85	0.252303376282768
1102	86-87	0.2806300952745815
1102	88-89	0.3653093103598266
1102	90-91	0.36256958541375184
1102	92-93	0.3517749087401256
1102	94-95	0.3458435009463514
1102	96-97	0.3727695741527839
1102	98-99	0.429370234634888
1102	100	0.42229142423802557
1103	1	0.18825268532104644
1103	2	0.17930687622199315
1103	3	0.17103193698840613
1103	4	0.12207813887827257
1103	5	0.11897943626992458
1103	6	0.1148836784228422
1103	7	0.17754745275257733
1103	8	0.19885608873185134
1103	9	0.22631379481725844
1103	10-11	0.25112972352211926
1103	12-13	0.2279746077359306
1103	14-15	0.33490595407417345
1103	16-17	0.4422969327809838
1103	18-19	0.33412224000618096
1103	20-21	0.3444848207955218
1103	22-23	0.3297590549966287
1103	24-25	0.37030691670648963
1103	26-27	0.3276585674936996
1103	28-29	0.31330238429526247
1103	30-31	0.3734034662932686
1103	32-33	0.34932169999459717
1103	34-35	0.3144661877423687
1103	36-37	0.3523488829021062
1103	38-39	0.3399466830120872
1103	40-41	0.31618046121813137
1103	42-43	0.3500451415588657
1103	44-45	0.36907413574500225
1103	46-47	0.3424063901430401
1103	48-49	0.4524146148968029
1103	50-51	0.43192456326530504
1103	52-53	0.39132495926033073
1103	54-55	0.3281265555547179
1103	56-57	0.3444421094702079
1103	58-59	0.3489450611107614
1103	60-61	0.37184377735201934
1103	62-63	0.3781030962017482
1103	64-65	0.3999716253883676
1103	66-67	0.3564206737792688
1103	68-69	0.34964155738113334
1103	70-71	0.36706413960527584
1103	72-73	0.33119507912013546
1103	74-75	0.34904569929521045
1103	76-77	0.298754072685675
1103	78-79	0.34245084804000214
1103	80-81	0.36541203942785216
1103	82-83	0.29971669105660936
1103	84-85	0.2900103843094115
1103	86-87	0.26339622986720457
1103	88-89	0.3578257994516605
1103	90-91	0.32464467420116705
1103	92-93	0.34650620484127614
1103	94-95	0.3055379161202225
1103	96-97	0.2892833833606545
1103	98-99	0.32983075838308196
1103	100	0.38439578360265614
1104	1	0.19925143407758839
1104	2	0.2242342900058958
1104	3	0.21163771742200765
1104	4	0.15245167780120283
1104	5	0.12234175389703239
1104	6	0.1702740680414223
1104	7	0.14593347417454083
1104	8	0.14709031842343734
1104	9	0.21819068616839843
1104	10-11	0.2329959357088498
1104	12-13	0.20077682391084295
1104	14-15	0.32620093126563177
1104	16-17	0.3968223514402709
1104	18-19	0.3111809291004164
1104	20-21	0.3137015955100608
1104	22-23	0.3361824357971983
1104	24-25	0.29318828057845536
1104	26-27	0.2769927030093342
1104	28-29	0.3108459542141304
1104	30-31	0.3206359246958925
1104	32-33	0.30895996169324036
1104	34-35	0.28085993461429126
1104	36-37	0.310735661996965
1104	38-39	0.2662058246393286
1104	40-41	0.2652512962617095
1104	42-43	0.26989235329028816
1104	44-45	0.2518589186911342
1104	46-47	0.3456731335110774
1104	48-49	0.3592760223457958
1104	50-51	0.2540815884811778
1104	52-53	0.26072217153616606
1104	54-55	0.27334175561510676
1104	56-57	0.29978870148823233
1104	58-59	0.3366938872312488
1104	60-61	0.28054076201931366
1104	62-63	0.22244135390241837
1104	64-65	0.27669917513699716
1104	66-67	0.3140980454100557
1104	68-69	0.3018047978780132
1104	70-71	0.2723438732510317
1104	72-73	0.3138864035517557
1104	74-75	0.2607393744822204
1104	76-77	0.26107512381232567
1104	78-79	0.23203161634956615
1104	80-81	0.2736195612205137
1104	82-83	0.26035448731958155
1104	84-85	0.29079582634373224
1104	86-87	0.2404073991134652
1104	88-89	0.2726961863231523
1104	90-91	0.23975346215722482
1104	92-93	0.21149041567545623
1104	94-95	0.23708920451159798
1104	96-97	0.23110215222634167
1104	98-99	0.2546240997973257
1104	100	0.24378581950926304
1105	1	0.13860891824182886
1105	2	0.13255007487062187
1105	3	0.12215707186958014
1105	4	0.10134447592373164
1105	5	0.07674139611814468
1105	6	0.037376565008813145
1105	7	0.09314172195343673
1105	8	0.09044272459960467
1105	9	0.11538654798465586
1105	10-11	0.11586303093981343
1105	12-13	0.1258848732361173
1105	14-15	0.25399537447252385
1105	16-17	0.29553056752158113
1105	18-19	0.19776718951723637
1105	20-21	0.1878079485081301
1105	22-23	0.182526850805786
1105	24-25	0.17968859151730499
1105	26-27	0.18187569489607114
1105	28-29	0.15567286933367797
1105	30-31	0.18386719721085854
1105	32-33	0.16644655693756505
1105	34-35	0.18514860017679524
1105	36-37	0.13278680280070887
1105	38-39	0.1761213043212848
1105	40-41	0.18188675829782852
1105	42-43	0.20367661345584764
1105	44-45	0.19045740305770664
1105	46-47	0.220536541504039
1105	48-49	0.2655028189242117
1105	50-51	0.2601873778105883
1105	52-53	0.26342663587684
1105	54-55	0.21882970379389377
1105	56-57	0.19684946703985418
1105	58-59	0.1801777944880385
1105	60-61	0.1501848866121307
1105	62-63	0.14460217618015747
1105	64-65	0.192931468754459
1105	66-67	0.20999032213636326
1105	68-69	0.13070941013714332
1105	70-71	0.10340745968505871
1105	72-73	0.09772813939194691
1105	74-75	0.12615453895756445
1105	76-77	0.07824536339231969
1105	78-79	0.12911350462016458
1105	80-81	0.12080718328558504
1105	82-83	0.06809757908977332
1105	84-85	0.030999078221434218
1105	86-87	0.0988689212851952
1105	88-89	0.0973480686531829
1105	90-91	0.053362322181641275
1105	92-93	0.10052693850423111
1105	94-95	0.11991024573252673
1105	96-97	0.09466370357282017
1105	98-99	0.1243996201272779
1105	100	0.1461037245871637
1106	1	0.07035760968970806
1106	2	0.04069738275523349
1106	3	0.06865893970062587
1106	4	0.09978603925582519
1106	5	0.07172871973104833
1106	6	0.09697429686470116
1106	7	0.06724273919819979
1106	8	0.096619676104865
1106	9	0.05633713967190346
1106	10-11	0.09966218393869042
1106	12-13	0.07024883636774604
1106	14-15	0.17959725299456863
1106	16-17	0.1993671300160571
1106	18-19	0.09337580376030985
1106	20-21	0.11050984313762058
1106	22-23	0.12090262511387095
1106	24-25	0.14948707829495333
1106	26-27	0.12610881248772898
1106	28-29	0.11317520259105152
1106	30-31	0.0770815805222611
1106	32-33	0.08632919831516261
1106	34-35	0.10977385718330623
1106	36-37	0.17480376113583418
1106	38-39	0.13979932524960503
1106	40-41	0.07928261480414278
1106	42-43	0.10408545095912558
1106	44-45	0.0830797186833081
1106	46-47	0.128970236822731
1106	48-49	0.25433220013158575
1106	50-51	0.23142952446669085
1106	52-53	0.1730899130172645
1106	54-55	0.20859701771138361
1106	56-57	0.2127969023653904
1106	58-59	0.19990035448502397
1106	60-61	0.17639036208400682
1106	62-63	0.16809871308070257
1106	64-65	0.1696893365421701
1106	66-67	0.1564218021340622
1106	68-69	0.11360897103209311
1106	70-71	0.10258007378833156
1106	72-73	0.1219016401136912
1106	74-75	0.09962494157004187
1106	76-77	0.029885878360950358
1106	78-79	0.08101747745752874
1106	80-81	0.07987432396749483
1106	82-83	0.07896071215308353
1106	84-85	0.08539145054289321
1106	86-87	0.11714475209183206
1106	88-89	0.09168035210130654
1106	90-91	0.09352932369992573
1106	92-93	0.07415444345930666
1106	94-95	0.0911451059969437
1106	96-97	0.10827179448531865
1106	98-99	0.08798076428679735
1106	100	0.0786641845506395
1107	1	0.0646370862286787
1107	2	0.04347657804949989
1107	3	0.05149749067448539
1107	4	0.035664793963718466
1107	5	0.055072236934300633
1107	6	0.0745341849978729
1107	7	0.0696289807191377
1107	8	0.10389393247503875
1107	9	0.09449904317978053
1107	10-11	0.09093049139164577
1107	12-13	0.13532704181412214
1107	14-15	0.1873046322853753
1107	16-17	0.23978566583243577
1107	18-19	0.20179979578472995
1107	20-21	0.15145852674854865
1107	22-23	0.16140513993306627
1107	24-25	0.14631134919762445
1107	26-27	0.17118581650039744
1107	28-29	0.1528981811029837
1107	30-31	0.1813853223814874
1107	32-33	0.1242592493882313
1107	34-35	0.1057111908863746
1107	36-37	0.07256430083442211
1107	38-39	0.1188821823075159
1107	40-41	0.11075732614148848
1107	42-43	0.12265767441851239
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2216	50-51	-0.5716940232780487
2216	52-53	-0.580434400469656
2216	54-55	-0.560927597429469
2216	56-57	-0.5686187242842777
2216	58-59	-0.5694347339880039
2216	60-61	-0.6174608881149055
2216	62-63	-0.6041335970859691
2216	64-65	-0.5689286244843856
2216	66-67	-0.5765922522514728
2216	68-69	-0.5510827012392241
2216	70-71	-0.5534284933888642
2216	72-73	-0.5391802942824029
2216	74-75	-0.5197409243355366
2216	76-77	-0.46484658823948166
2216	78-79	-0.472980734018833
2216	80-81	-0.4933799202237594
2216	82-83	-0.4713270874432425
2216	84-85	-0.4667314475844435
2216	86-87	-0.41604408010426397
2216	88-89	-0.34992076432641994
2216	90-91	-0.419219680966215
2216	92-93	-0.4235076871847525
2216	94-95	-0.4733544716105129
2216	96-97	-0.44942038513642046
2216	98-99	-0.49952418017558387
2216	100	-0.5758645577878561
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	25.0
8	129.0
9	223.0
10	213.0
11	148.0
12	151.0
13	242.0
14	420.0
15	730.0
16	1367.0
17	2367.0
18	4050.0
19	6217.0
20	8960.0
21	13485.0
22	19376.0
23	22960.0
24	22902.0
25	21869.0
26	23289.0
27	27539.0
28	34710.0
29	43520.0
30	55422.0
31	70820.0
32	90004.0
33	117814.0
34	168801.0
35	257657.0
36	473992.0
37	1044293.0
38	1465102.0
39	462159.0
40	1176.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.85657616775953	19.469001162257513	13.412397921472987	24.262024748509976
2	14.831807022836163	21.25992669585863	39.3428176598704	24.565448621434804
3	19.475810292181418	25.691189735832143	29.94012942264056	24.892870549345883
4	12.401586499729476	17.65233197926133	38.94722751571174	30.99885400529745
5	12.83441934995882	38.28521803441263	35.34182349942011	13.538539116208447
6	29.985360641012075	37.743167552080436	18.213182415556986	14.058289391350495
7	26.267734795833025	32.75051029418224	22.98847277489774	17.993282135086993
8	25.041247228334168	34.45661903158993	20.790306307453097	19.7118274326228
9	25.486740855484197	16.584867130660527	21.240605379316772	36.6877866345385
10-11	24.41068450330627	26.008487882233716	28.56513912874715	21.015688485712865
12-13	26.18284264114105	23.696239824370952	28.11756801457241	22.00334951991559
14-15	23.040529630408614	25.06548161242707	26.823856899747778	25.070131857416538
16-17	21.881479892799845	27.989551156227176	27.5871191734961	22.541849777476873
18-19	21.60828322921066	28.126310684518103	28.84442153059602	21.42098455567522
20-21	22.740797174617498	26.678576547052785	28.264246054259168	22.316380224070546
22-23	22.161648288307024	26.719805689298298	28.010847729291736	23.10769829310294
24-25	22.736732127153566	26.825002039383865	28.00232041544266	22.435945418019905
26-27	22.678625854974765	26.23694566708201	28.73265398510125	22.351774492841965
28-29	21.925203019543122	27.558619960144615	28.39016192259664	22.12601509771562
30-31	22.608099913001034	26.55684896109967	28.25356411142501	22.58148701447428
32-33	22.398356656317333	26.86626452616042	28.323161043109085	22.41221777441316
34-35	22.784654669097197	26.80119297393216	28.430248271787935	21.98390408518271
36-37	22.826800205283128	26.274805978310805	28.6716769793798	22.226716837026263
38-39	22.566662875475043	26.841715135090617	28.55358942739373	22.03803256204061
40-41	22.53253647519698	26.835917781003708	28.367492266826833	22.264053476972485
42-43	22.52191329167313	26.744928209205828	27.655916499108496	23.07724200001255
44-45	22.84786537670907	26.38379305039622	27.853915764748542	22.91442580814617
46-47	23.094980157467326	26.931016384096214	27.844077674080463	22.129925784355997
48-49	23.07475870823098	26.797477168409966	27.777872401268546	22.349891722090508
50-51	21.970495269974084	27.093181017504637	28.164473843445244	22.77184986907604
52-53	22.41574748516513	27.53476933172154	26.960490995091575	23.088992188021752
54-55	22.331419220330073	26.866394643441826	27.330243769832226	23.471942366395876
56-57	22.994434829574008	26.345482059847015	27.41642859824153	23.243654512337443
58-59	23.345970127980042	26.481555105643036	27.398891822732875	22.77358294364405
60-61	22.744828141071103	26.968720701184836	28.008443101231716	22.27800805651235
62-63	22.127672115004383	26.88347739210831	27.81724763232231	23.171602860564995
64-65	21.844948761445938	27.517781071928056	27.448464221647185	23.188805944978828
66-67	22.691538303626736	26.557545756646444	27.101137047173474	23.649778892553343
68-69	22.402769052926793	26.636278412229768	27.34214531454398	23.618807220299455
70-71	22.77879333260645	26.492478064968196	27.28034516488015	23.44838343754521
72-73	22.823305745598336	25.945443384557663	28.05359785405984	23.177653015784152
74-75	22.344989350105422	27.277053690529122	27.183710233221937	23.19424672614352
76-77	22.421710439986324	26.430723996965	27.220278687850403	23.927286875198273
78-79	22.53706349875125	26.55202560604132	27.447058227657468	23.463852667549965
80-81	22.67163175458493	26.460484488264974	27.38711314508595	23.48077061206415
82-83	22.486742516560298	26.104969574320624	27.88002964969989	23.528258259419186
84-85	22.378512310939996	26.669275298886163	27.28966586088169	23.66254652929215
86-87	22.16922314265916	26.82763530975776	27.214782081749263	23.78835946583382
88-89	22.209047155037098	26.481556350193138	26.89562971243343	24.413766782336335
90-91	22.525497912431955	26.00373588398366	27.007662259952088	24.4631039436323
92-93	22.727130622273513	26.482434003764343	27.1183075255884	23.67212784837374
94-95	22.20682598078764	26.276498567499633	27.465247523154872	24.051427928557853
96-97	21.79205721416226	26.22012849520586	27.90653842843927	24.081275862192612
98-99	22.160231378365726	26.58186558275944	26.93497060875768	24.32293243011715
100	22.140684757712176	25.557206937778925	26.89516294522562	25.406945359283284
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	124.0
1	76.0
2	103.5
3	192.5
4	147.5
5	113.5
6	203.5
7	497.0
8	984.5
9	1243.5
10	1107.0
11	937.5
12	996.5
13	1161.5
14	1428.0
15	1740.5
16	2025.0
17	2385.5
18	2800.5
19	3260.0
20	3952.0
21	4857.0
22	6128.0
23	7915.0
24	9954.5
25	13047.5
26	17890.0
27	23537.5
28	29643.5
29	37096.5
30	45832.0
31	54665.5
32	64073.5
33	73321.0
34	81700.0
35	90667.5
36	100857.0
37	113155.5
38	121387.0
39	125081.5
40	129558.5
41	133819.5
42	137264.0
43	143170.0
44	154724.5
45	165582.5
46	173381.0
47	199563.5
48	247150.0
49	247643.5
50	217858.5
51	195345.0
52	170280.0
53	158500.0
54	147926.5
55	132863.5
56	118173.5
57	103939.5
58	89844.5
59	78538.0
60	65193.5
61	50794.0
62	39339.5
63	30701.5
64	22877.5
65	16333.0
66	11885.5
67	8654.0
68	6273.0
69	4558.5
70	3538.5
71	2674.5
72	1937.0
73	1645.5
74	1357.0
75	1023.0
76	724.5
77	439.5
78	285.0
79	201.5
80	155.0
81	96.5
82	47.0
83	17.5
84	9.0
85	5.0
86	4.5
87	2.5
88	2.0
89	1.5
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0028237622732810233
2	0.0038994812345309374
3	1.5687568184894575E-4
4	0.05044673712028241
5	0.0023979568511195994
6	0.004213232598228828
7	0.0
8	3.137513636978915E-4
9	8.964324677082614E-5
10-11	1.344648701562392E-4
12-13	0.0
14-15	3.36162175390598E-5
16-17	2.577243344661252E-4
18-19	1.68081087695299E-4
20-21	2.3531352277341862E-4
22-23	0.0
24-25	0.0
26-27	2.689297403124784E-4
28-29	0.0
30-31	2.2410811692706535E-4
32-33	2.2410811692706535E-4
34-35	1.344648701562392E-4
36-37	4.482162338541307E-5
38-39	7.731730033983754E-4
40-41	5.490648864713101E-4
42-43	0.0
44-45	4.8183245139319053E-4
46-47	0.0
48-49	6.72324350781196E-5
50-51	0.0
52-53	2.2410811692706535E-4
54-55	2.9134055200518496E-4
56-57	1.0084865261717942E-4
58-59	6.72324350781196E-4
60-61	6.162973215494297E-4
62-63	1.4567027600259248E-4
64-65	3.0254595785153825E-4
66-67	0.0010757189612499137
68-69	0.0022634919809633603
70-71	0.0033280055363669207
72-73	0.0028013514615883167
74-75	0.0025996541563539583
76-77	0.004179616380689769
78-79	0.001344648701562392
80-81	9.524594969400278E-4
82-83	6.611189449348429E-4
84-85	5.266540747786035E-4
86-87	0.0
88-89	0.0
90-91	4.482162338541307E-5
92-93	5.602702923176634E-5
94-95	8.964324677082614E-5
96-97	4.482162338541307E-4
98-99	7.843784092447287E-5
100	0.07263344069606188
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4462132.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.83857348032737
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.84275229372798	43.58806385637528
2	15.093533431993587	18.06351018696252
3	5.505305181582372	9.882888259192312
4	2.575082221149167	6.163569868324761
5	1.3018683477105042	3.895097239309369
6	0.7931995421867866	2.8478357453823637
7	0.5172184802945547	2.1664731226942275
8	0.3228438152766143	1.5454810690479137
9	0.22914049729781724	1.2340296436386773
>10	0.7953527046166469	7.2605174506900045
>50	0.016815910530570834	0.6769704829290253
>100	0.00571628306895125	0.6078864705152531
>500	5.8564528239756E-4	0.24646570569746198
>1k	2.342581128343863E-4	0.2726086625718076
>5k	3.5138716925157943E-4	1.5486022366690035
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	9822	0.2201189924457636	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	7950	0.17816595295701695	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7920	0.17749362860623577	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7053	0.15806345486865922	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	6407	0.14358607051517078	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	5038	0.11290566930785553	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	2.2410811692706535E-5
14-15	0.0	0.0	0.0	1.1205405846353268E-5	3.36162175390598E-5
16-17	0.0	0.0	0.0	2.2410811692706535E-5	4.482162338541307E-5
18-19	0.0	0.0	0.0	5.602702923176634E-5	6.72324350781196E-5
20-21	0.0	0.0	0.0	1.0084865261717941E-4	6.72324350781196E-5
22-23	0.0	0.0	0.0	3.137513636978915E-4	6.72324350781196E-5
24-25	0.0	0.0	0.0	7.171459741666091E-4	6.72324350781196E-5
26-27	0.0	0.0	0.0	0.001423086542486865	8.964324677082614E-5
28-29	0.0	0.0	0.0	0.0037538109585283446	8.964324677082614E-5
30-31	0.0	0.0	0.0	0.011519157210051159	8.964324677082614E-5
32-33	0.0	0.0	0.0	0.03170009313933339	8.964324677082614E-5
34-35	0.0	0.0	0.0	0.062089153794643456	8.964324677082614E-5
36-37	0.0	0.0	0.0	0.11194200440506916	8.964324677082614E-5
38-39	0.0	0.0	0.0	0.18956185070275824	8.964324677082614E-5
40-41	0.0	0.0	0.0	0.27846554068772505	8.964324677082614E-5
42-43	0.0	0.0	0.0	0.36710030093237944	8.964324677082614E-5
44-45	0.0	0.0	0.0	0.4780226134054304	8.964324677082614E-5
46-47	0.0	0.0	0.0	0.6161852674909662	1.0084865261717941E-4
48-49	0.0	0.0	0.0	0.7581353487525695	1.2325946430988594E-4
50-51	0.0	0.0	0.0	0.9101030628408124	1.344648701562392E-4
52-53	0.0	0.0	0.0	1.0644575283743287	1.344648701562392E-4
54-55	0.0	0.0	0.0	1.2381189081811117	1.344648701562392E-4
56-57	0.0	0.0	0.0	1.4538005599117194	1.4567027600259248E-4
58-59	0.0	0.0	0.0	1.6869850555743309	1.5687568184894575E-4
60-61	0.0	0.0	0.0	1.9334591625707174	1.5687568184894575E-4
62-63	0.0	0.0	0.0	2.1893794266955795	1.5687568184894575E-4
64-65	0.0	0.0	0.0	2.4559560317803237	1.5687568184894575E-4
66-67	0.0	0.0	0.0	2.7597457896808075	1.5687568184894575E-4
68-69	0.0	0.0	0.0	3.1014546409653505	1.5687568184894575E-4
70-71	0.0	0.0	0.0	3.4825056721764396	1.5687568184894575E-4
72-73	0.0	0.0	0.0	3.8563852436458625	1.68081087695299E-4
74-75	0.0	0.0	0.0	4.232539512502095	1.7928649354165228E-4
76-77	0.0	0.0	0.0	4.642354820520773	1.7928649354165228E-4
78-79	0.0	0.0	0.0	5.109373725385085	1.7928649354165228E-4
80-81	0.0	0.0	0.0	5.616149410192258	1.7928649354165228E-4
82-83	0.0	0.0	0.0	6.126678905957959	1.7928649354165228E-4
84-85	0.0	0.0	0.0	6.63438463945038	1.7928649354165228E-4
86-87	0.0	0.0	0.0	7.161509341274529	2.1290271108071208E-4
88	0.0	0.0	0.0	7.582384384863558	2.2410811692706535E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGTACT	10425	0.0	21.075672	12-13
CTAGTAC	2515	0.0	20.1958	3
GTACTAG	1820	0.0	20.157763	1
GTACTTT	11665	0.0	18.754942	14-15
CGTTAAC	510	0.0	18.445013	1
AGTACTT	11230	0.0	18.288479	12-13
CTGTGCG	2245	0.0	17.375416	9
CATGGGG	7510	0.0	17.221384	4
GTATAAG	2205	0.0	16.851463	1
TAGTACT	2775	0.0	16.26985	4
TGATCGC	955	0.0	16.239927	9
TAGGCAT	2505	0.0	16.135908	5
ACTTTTT	14350	0.0	15.556966	16-17
ACTGTGC	4400	0.0	15.487767	8
TACGGGC	1005	0.0	15.442698	4
CGGGCGT	1050	0.0	15.219046	6
TACCTGG	4295	0.0	15.1111	2
GTGATCG	1060	0.0	15.074626	8
CCTAGTA	2875	0.0	15.04978	2
GGCGTCG	1070	0.0	14.933741	8
>>END_MODULE