FastQCFastQC Report
Mon 16 Jan 2017
ERR1146636_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146636_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6035283
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1130701.8734829833166065No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT293170.4857601540805957No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC262670.4352239986095101No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC217750.3607950116009473No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA193720.32097914878225264No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG188250.3119157792600612No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC145820.2416125308456952No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG21000.021.7229271
CATGGGG112350.020.1266784
GTACGCA16000.018.5109143
GAGTACG18100.017.9281881
ATGGGGG78500.017.7857635
CTAGTAC31900.017.5373363
TAGTACT30950.017.4677644
GTATAAG27100.017.353881
TACCTGG54000.017.324882
TACGCAG16900.017.2466644
GGCGTCG16950.017.1890878
GTGATCG16700.016.883628
CTGTGCG34300.016.8507889
ATACGGG17600.016.8281043
TGGGGGG75600.016.2246276
TGATCGC16300.016.1439429
ACGGGCG18350.016.1395035
CGACGAG10500.016.11139924-25
TCTAACG10250.016.052962
TACGGGC18200.016.014764
Produced by FastQC (version 0.11.5)