##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146636_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6035283
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.339195858752607	31.0	31.0	34.0	30.0	34.0
2	31.53076135783525	31.0	31.0	34.0	30.0	34.0
3	31.716090198255824	33.0	31.0	34.0	30.0	34.0
4	35.249876434957564	37.0	35.0	37.0	33.0	37.0
5	35.373735912632434	37.0	35.0	37.0	33.0	37.0
6	35.44697340621807	37.0	35.0	37.0	33.0	37.0
7	35.3775037558305	37.0	35.0	37.0	33.0	37.0
8	35.38908647697217	37.0	35.0	37.0	33.0	37.0
9	37.08425586670915	39.0	37.0	39.0	34.0	39.0
10-11	37.08054576728216	39.0	37.0	39.0	34.0	39.0
12-13	37.056050312802235	39.0	37.0	39.0	34.0	39.0
14-15	38.37920168780818	40.0	38.0	41.0	34.0	41.0
16-17	38.42162331078758	40.0	38.0	41.0	34.0	41.0
18-19	38.427325528894	40.0	38.5	41.0	34.0	41.0
20-21	38.483803327863825	40.0	39.0	41.0	34.0	41.0
22-23	38.463485970749005	40.0	39.0	41.0	34.0	41.0
24-25	38.44199733467345	40.0	38.0	41.0	34.0	41.0
26-27	38.428549498010284	40.0	38.0	41.0	34.0	41.0
28-29	38.41694913063729	40.0	38.0	41.0	34.0	41.0
30-31	38.40137098790562	40.0	38.0	41.0	34.0	41.0
32-33	38.32999430184135	40.0	38.0	41.0	33.0	41.0
34-35	38.252162740338775	40.0	38.0	41.0	33.0	41.0
36-37	38.17455005838168	40.0	38.0	41.0	33.0	41.0
38-39	38.102954161387295	40.0	38.0	41.0	33.0	41.0
40-41	38.098059444768374	40.0	38.0	41.0	33.0	41.0
42-43	37.95387846767086	40.0	38.0	41.0	33.0	41.0
44-45	37.82038041960915	40.0	38.0	41.0	32.0	41.0
46-47	37.6750681782445	40.0	37.0	41.0	32.0	41.0
48-49	37.54294678476552	40.0	37.0	41.0	31.5	41.0
50-51	37.37314074584407	40.0	37.0	41.0	31.0	41.0
52-53	37.31494446904975	40.0	36.5	41.0	31.0	41.0
54-55	37.18812970327986	39.5	36.0	41.0	31.0	41.0
56-57	36.919333360175486	39.0	35.5	41.0	30.5	41.0
58-59	36.70751785790327	39.0	35.0	41.0	30.0	41.0
60-61	36.395040133163604	39.0	35.0	40.5	30.0	41.0
62-63	36.20390369432552	38.0	35.0	40.0	30.0	41.0
64-65	35.929550445273236	37.5	35.0	40.0	29.0	41.0
66-67	35.620488219028005	37.0	34.5	40.0	29.0	41.0
68-69	35.28138937312467	36.5	34.0	39.0	29.0	41.0
70-71	34.89760612716918	36.0	34.0	39.0	28.5	41.0
72-73	34.51345496143263	35.5	34.0	38.5	28.0	40.5
74-75	34.21643202149758	35.0	34.0	37.5	28.0	40.0
76-77	33.94407403927869	35.0	34.0	37.0	28.0	39.0
78-79	33.59619540624689	35.0	33.0	36.5	27.0	39.0
80-81	33.30361865383943	35.0	33.0	36.0	26.5	38.5
82-83	33.03518923636224	35.0	33.0	36.0	26.0	37.0
84-85	32.86717068611364	35.0	33.0	35.0	26.0	37.0
86-87	32.77145305365134	35.0	33.0	35.0	26.0	36.5
88-89	32.59713355612322	35.0	33.0	35.0	26.0	36.0
90-91	32.42477163042727	35.0	33.0	35.0	26.0	36.0
92-93	32.25342092491769	35.0	33.0	35.0	25.0	36.0
94-95	32.16901676358839	35.0	32.5	35.0	25.0	35.0
96-97	32.086977362950506	35.0	32.0	35.0	25.0	35.0
98-99	32.08678648540591	35.0	32.0	35.0	25.0	35.0
100	30.85141326429929	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.12204971070499937
1101	2	0.21701458091837722
1101	3	0.15567177161729617
1101	4	0.12214942356278158
1101	5	0.1621095410350435
1101	6	0.20555938171677468
1101	7	0.24287968007827487
1101	8	0.27999428726107567
1101	9	0.32585548914606477
1101	10-11	0.3995308897219516
1101	12-13	0.36949920517589874
1101	14-15	0.4629266135781265
1101	16-17	0.4894705680727469
1101	18-19	0.4246489303118892
1101	20-21	0.47274307717224673
1101	22-23	0.48337219260440634
1101	24-25	0.4942181289316707
1101	26-27	0.46931693055887536
1101	28-29	0.4629798691256397
1101	30-31	0.45375338183963265
1101	32-33	0.5433561807115623
1101	34-35	0.47372253663908026
1101	36-37	0.4816044533795534
1101	38-39	0.4626197852857672
1101	40-41	0.45201932445902315
1101	42-43	0.47640681283417763
1101	44-45	0.3854414016982233
1101	46-47	0.37828834589642923
1101	48-49	0.4492358349178005
1101	50-51	0.39802168460722953
1101	52-53	0.36423020902353187
1101	54-55	0.36175800412948433
1101	56-57	0.4048817353774723
1101	58-59	0.4259949037002926
1101	60-61	0.3992051970102821
1101	62-63	0.4330925132078036
1101	64-65	0.38352064537153296
1101	66-67	0.3119678471554934
1101	68-69	0.3293328716819346
1101	70-71	0.31644542126973363
1101	72-73	0.3810975300626751
1101	74-75	0.3322281930901596
1101	76-77	0.3138452720871783
1101	78-79	0.3305651945920829
1101	80-81	0.3734081313949602
1101	82-83	0.4064040799586195
1101	84-85	0.3353183442585106
1101	86-87	0.30906597788840173
1101	88-89	0.3076915989889386
1101	90-91	0.2966780831932212
1101	92-93	0.35354798671020404
1101	94-95	0.38281512528625683
1101	96-97	0.32764569233552265
1101	98-99	0.33758543089724213
1101	100	0.2918541730615445
1102	1	0.21834801879423438
1102	2	0.262371136697368
1102	3	0.2776211931537915
1102	4	0.3154805714436293
1102	5	0.32923560352141834
1102	6	0.39189914018140826
1102	7	0.3533450399657809
1102	8	0.3708791054982399
1102	9	0.47188435885098556
1102	10-11	0.47711189947136035
1102	12-13	0.4651134909996273
1102	14-15	0.6208484476008849
1102	16-17	0.5292093096961423
1102	18-19	0.49953724670620403
1102	20-21	0.5426903609156355
1102	22-23	0.5409873271508374
1102	24-25	0.5704325305763049
1102	26-27	0.5186562858860881
1102	28-29	0.5432815092547116
1102	30-31	0.5834305811000533
1102	32-33	0.5620870224987655
1102	34-35	0.5243658249834127
1102	36-37	0.5611429603123952
1102	38-39	0.5764204096367322
1102	40-41	0.5357260080975408
1102	42-43	0.5948603051440031
1102	44-45	0.5443257194727593
1102	46-47	0.5617006774612818
1102	48-49	0.5697447359137513
1102	50-51	0.5694801258842546
1102	52-53	0.5211852695590267
1102	54-55	0.5167652413725392
1102	56-57	0.5991088809956793
1102	58-59	0.6364793944400944
1102	60-61	0.565815533619471
1102	62-63	0.48443112956700674
1102	64-65	0.5915238221600774
1102	66-67	0.5495508608660771
1102	68-69	0.4901469743622968
1102	70-71	0.48996402023391283
1102	72-73	0.47961579092924467
1102	74-75	0.4671818354136974
1102	76-77	0.464222086752784
1102	78-79	0.4343285512063346
1102	80-81	0.3849478228029213
1102	82-83	0.412138088293716
1102	84-85	0.3973651273516623
1102	86-87	0.3400039802467205
1102	88-89	0.3892225952300805
1102	90-91	0.37537612443158963
1102	92-93	0.36366965181706234
1102	94-95	0.3849290658986604
1102	96-97	0.4109552578632929
1102	98-99	0.45918264462902414
1102	100	0.4851872752638009
1103	1	0.11503903709519037
1103	2	0.13805399497487514
1103	3	0.1850701102723491
1103	4	0.19291918215945003
1103	5	0.22776244980946103
1103	6	0.28963852765960496
1103	7	0.2654625684147618
1103	8	0.2602274028143228
1103	9	0.36674398139080466
1103	10-11	0.3169557787446138
1103	12-13	0.35840587844472793
1103	14-15	0.4512271606243061
1103	16-17	0.4761317788360273
1103	18-19	0.41951754398446184
1103	20-21	0.3914978262024462
1103	22-23	0.44679499100136866
1103	24-25	0.4712749194397148
1103	26-27	0.45014426687124853
1103	28-29	0.45053806776718375
1103	30-31	0.4842528772058401
1103	32-33	0.5089034136976665
1103	34-35	0.46790753991209044
1103	36-37	0.48981692104405283
1103	38-39	0.5268650209955297
1103	40-41	0.4263906765197589
1103	42-43	0.5220044092197611
1103	44-45	0.44734152419952267
1103	46-47	0.5044550813209838
1103	48-49	0.4178297483712541
1103	50-51	0.49454920951266956
1103	52-53	0.46455294574887773
1103	54-55	0.42385854622931163
1103	56-57	0.5150963336595069
1103	58-59	0.4744459305432329
1103	60-61	0.48485014162158535
1103	62-63	0.4289302053001549
1103	64-65	0.4140443463210204
1103	66-67	0.4694132609341466
1103	68-69	0.42573476434807134
1103	70-71	0.4785627099497347
1103	72-73	0.48353240125739205
1103	74-75	0.45464602302804735
1103	76-77	0.40204962825023216
1103	78-79	0.38486357997443577
1103	80-81	0.3729781534115162
1103	82-83	0.36697075108051536
1103	84-85	0.36360013460868856
1103	86-87	0.33496542579062094
1103	88-89	0.31092968773713636
1103	90-91	0.3684371792304475
1103	92-93	0.34576404995623733
1103	94-95	0.3863172390651357
1103	96-97	0.4254328284361435
1103	98-99	0.39292603765754563
1103	100	0.37017278483977023
1104	1	0.04704152004565998
1104	2	0.09123819382278597
1104	3	0.13192887289759625
1104	4	0.13310239660262368
1104	5	0.15652967886888547
1104	6	0.19789921321429915
1104	7	0.21852475430716822
1104	8	0.2507764881256236
1104	9	0.2728280006187873
1104	10-11	0.3149426473308594
1104	12-13	0.30468910655176984
1104	14-15	0.37177739030117607
1104	16-17	0.3952965570030358
1104	18-19	0.3701628011327003
1104	20-21	0.42632320273627045
1104	22-23	0.39030662433056307
1104	24-25	0.4133511134998784
1104	26-27	0.43715308237316464
1104	28-29	0.406887060000777
1104	30-31	0.41420392675443196
1104	32-33	0.39847841958090413
1104	34-35	0.36817757922239736
1104	36-37	0.41740953658545266
1104	38-39	0.40644408914594976
1104	40-41	0.4406757863406483
1104	42-43	0.4190694261157901
1104	44-45	0.3682709526175785
1104	46-47	0.4399651567450107
1104	48-49	0.3849962893355965
1104	50-51	0.39387546016523345
1104	52-53	0.42710275068625947
1104	54-55	0.35647531862296944
1104	56-57	0.44762610099950706
1104	58-59	0.3833362312775037
1104	60-61	0.44037211232055995
1104	62-63	0.3758283487267775
1104	64-65	0.37185427405140814
1104	66-67	0.3878165945889904
1104	68-69	0.3379815578186083
1104	70-71	0.356537088678472
1104	72-73	0.3592537732100709
1104	74-75	0.32241529778093536
1104	76-77	0.31266528902153823
1104	78-79	0.34429045112744205
1104	80-81	0.19526739546598293
1104	82-83	0.2705139345092107
1104	84-85	0.2959952314666836
1104	86-87	0.2823064920573586
1104	88-89	0.24838142113803485
1104	90-91	0.28816004149363295
1104	92-93	0.2941367252207314
1104	94-95	0.32633676212623897
1104	96-97	0.28745221795499987
1104	98-99	0.28914444144083973
1104	100	0.3578204719036826
1105	1	0.04204111753521289
1105	2	0.03806176819828622
1105	3	0.043770993890827725
1105	4	0.11801327769867243
1105	5	0.08204133646690792
1105	6	0.16239951910860384
1105	7	0.13422583909441244
1105	8	0.19602252155676325
1105	9	0.2510392078278443
1105	10-11	0.24863667638953046
1105	12-13	0.2655615455591587
1105	14-15	0.34770256090446594
1105	16-17	0.2946710816781959
1105	18-19	0.29591346493401716
1105	20-21	0.2962383946260232
1105	22-23	0.327691294632686
1105	24-25	0.38614868020035686
1105	26-27	0.3249525338952992
1105	28-29	0.2551939410566533
1105	30-31	0.2875670035870215
1105	32-33	0.38418043484684716
1105	34-35	0.31294003192060416
1105	36-37	0.34936422504577536
1105	38-39	0.38420471647859955
1105	40-41	0.36363853687385017
1105	42-43	0.3128967840894816
1105	44-45	0.28192966621418947
1105	46-47	0.310020215901325
1105	48-49	0.3191456711218237
1105	50-51	0.3622773900516876
1105	52-53	0.34040675053098823
1105	54-55	0.32597852709260877
1105	56-57	0.3147166513951589
1105	58-59	0.383189545652165
1105	60-61	0.38712463939751274
1105	62-63	0.31018632683612424
1105	64-65	0.33604798107899114
1105	66-67	0.36035167720408623
1105	68-69	0.3410227049320227
1105	70-71	0.25808914714325937
1105	72-73	0.26391541238261595
1105	74-75	0.3077598492236149
1105	76-77	0.2580845314936937
1105	78-79	0.2694817595018506
1105	80-81	0.22684273771691466
1105	82-83	0.251132249648208
1105	84-85	0.21591585758838505
1105	86-87	0.17103928598578477
1105	88-89	0.21021781698267716
1105	90-91	0.1647717253696115
1105	92-93	0.22445291660915956
1105	94-95	0.20765854552343654
1105	96-97	0.22156503061027877
1105	98-99	0.2531582119800646
1105	100	0.26434181843499616
1106	1	0.008534159906332661
1106	2	0.035084240261376465
1106	3	0.023456566629484144
1106	4	0.04889520754433363
1106	5	0.05912588012693476
1106	6	0.11019407388796765
1106	7	0.09822472780901847
1106	8	0.1516291539014034
1106	9	0.19522810744221175
1106	10-11	0.18726221898436535
1106	12-13	0.21096063139538757
1106	14-15	0.280787877848077
1106	16-17	0.2650134041738994
1106	18-19	0.3228840424818529
1106	20-21	0.27215570105763476
1106	22-23	0.2830550223966952
1106	24-25	0.27975081890750886
1106	26-27	0.26160650013686393
1106	28-29	0.2429878688181688
1106	30-31	0.24236351216378438
1106	32-33	0.2603719218319114
1106	34-35	0.23591053673897733
1106	36-37	0.24028858550017418
1106	38-39	0.21258172012082355
1106	40-41	0.22112534821192043
1106	42-43	0.2445696972341338
1106	44-45	0.18914956778362324
1106	46-47	0.28506028085760704
1106	48-49	0.2905579940942786
1106	50-51	0.24543797434990466
1106	52-53	0.22906121850209615
1106	54-55	0.19901520808314643
1106	56-57	0.25621288252373375
1106	58-59	0.23301563824768579
1106	60-61	0.27615567106101224
1106	62-63	0.2322393065878714
1106	64-65	0.31521359149000716
1106	66-67	0.32838558855364397
1106	68-69	0.23637643787022
1106	70-71	0.20310122379221696
1106	72-73	0.24115038557651758
1106	74-75	0.28424608934438567
1106	76-77	0.28484809374055686
1106	78-79	0.27575221889457424
1106	80-81	0.2391088679490636
1106	82-83	0.17989099938903053
1106	84-85	0.23574758772555526
1106	86-87	0.2398014817313907
1106	88-89	0.2191492409832847
1106	90-91	0.18892819294701724
1106	92-93	0.16104996570930297
1106	94-95	0.12576066451381962
1106	96-97	0.13698824035294166
1106	98-99	0.21029640600702493
1106	100	0.2592345765561035
1107	1	0.040742927614889624
1107	2	0.03396263440534142
1107	3	0.06793455240675073
1107	4	0.12004601337731913
1107	5	0.05614108492828507
1107	6	0.12114974876027418
1107	7	0.16896432654142046
1107	8	0.12664755275712736
1107	9	0.18856261871467694
1107	10-11	0.16618005211460485
1107	12-13	0.16337692989503694
1107	14-15	0.17691793410159562
1107	16-17	0.17394077747165682
1107	18-19	0.1842970423178869
1107	20-21	0.22847243082082258
1107	22-23	0.2558874516305494
1107	24-25	0.2736978069806426
1107	26-27	0.23210924412191503
1107	28-29	0.2436047879848573
1107	30-31	0.2952809014101021
1107	32-33	0.29903571863518863
1107	34-35	0.2980814625427044
1107	36-37	0.3190468719031401
1107	38-39	0.2527508889450587
1107	40-41	0.2564257381526289
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2216	56-57	-0.8421475099900917
2216	58-59	-0.8469210840360901
2216	60-61	-0.8779158505151017
2216	62-63	-0.8625814046543496
2216	64-65	-0.7827049862030862
2216	66-67	-0.7665921221291256
2216	68-69	-0.8339627685865096
2216	70-71	-0.8620152822455296
2216	72-73	-0.7491712055081337
2216	74-75	-0.6990992786870223
2216	76-77	-0.8149504103475778
2216	78-79	-0.7086169008477796
2216	80-81	-0.8175340561399693
2216	82-83	-0.8619051842508583
2216	84-85	-0.7911161848108037
2216	86-87	-0.7311972067396084
2216	88-89	-0.7291602085545463
2216	90-91	-0.7937763485027389
2216	92-93	-0.7282608895516773
2216	94-95	-0.8849250313910417
2216	96-97	-0.8227201333466958
2216	98-99	-0.7862672814611464
2216	100	-0.8468631896484098
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1773.0
1	423.0
2	330.0
3	310.0
4	240.0
5	301.0
6	393.0
7	946.0
8	2418.0
9	3118.0
10	3320.0
11	4528.0
12	6260.0
13	8371.0
14	10638.0
15	11648.0
16	12892.0
17	14397.0
18	16069.0
19	17469.0
20	19891.0
21	22407.0
22	25759.0
23	30075.0
24	35947.0
25	43823.0
26	49487.0
27	52439.0
28	59877.0
29	73505.0
30	88421.0
31	107283.0
32	136921.0
33	185324.0
34	275417.0
35	427184.0
36	759746.0
37	1435551.0
38	1620949.0
39	468606.0
40	827.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.715414118378774	19.039502435990464	13.087044677101689	23.158038768529078
2	14.5308748822835	22.06497950738132	38.37443462920961	25.029710981125568
3	21.756188109781778	25.047641663057195	29.5299378021755	23.666232424985523
4	12.177129542523524	16.839683990288414	40.612381994258676	30.370804472929386
5	12.800373578600421	39.10703637151171	35.020489777995344	13.072100271892523
6	29.440633860616273	37.77501337928699	18.038496193018624	14.745856567078114
7	26.482002572435142	32.42009564045874	22.954157852527427	18.14374393457869
8	24.92931602589648	32.46759664869489	20.757564375111215	21.845522950297422
9	24.65505454344067	18.10558026265151	22.206672623784154	35.03269257012367
10-11	25.13645969819633	25.19234281856716	28.290000561318585	21.381196921917933
12-13	27.49591701720905	23.368382044492492	27.02903933425411	22.10666160404435
14-15	23.388818852004025	25.187733628054808	27.150679809188293	24.27276771075287
16-17	21.947574405678626	27.909547024564667	27.42052216323751	22.722356406519193
18-19	21.655344196139414	29.87277476475362	26.90664566670083	21.56523537240614
20-21	23.654493896702405	26.86208693016517	25.64330935390172	23.8401098192307
22-23	22.533624563124224	27.140723434366965	26.052458671168043	24.273193331340767
24-25	24.355633402571524	27.075796290985448	25.65007495483682	22.918495351606214
26-27	23.706728991162436	25.669939001151633	27.993784794617092	22.629547213068843
28-29	22.113803706465596	29.262253857025527	26.23166214215796	22.392280294350922
30-31	23.264570952543583	26.691546192995506	25.904216891495302	24.139665962965605
32-33	23.279264778653523	27.297210168047943	26.44610844378878	22.97741660950976
34-35	24.352256378224414	26.758563413091665	26.517644153754826	22.371536054929095
36-37	23.679560136682003	25.95906670834677	27.93310342940487	22.42826972556635
38-39	23.101289858874733	27.389053198013517	27.24411861965299	22.265538323458756
40-41	22.885557247411942	27.705833344665198	26.509710964522863	22.898898443399997
42-43	22.530088432761318	27.644209879712605	25.16281386134279	24.662887826183287
44-45	23.594300236730056	26.1011181452967	25.953317330215626	24.351264287757616
46-47	24.516945554187743	27.289063717160932	26.39880098804396	21.79518974060737
48-49	23.953914689885394	26.731028857757405	26.790956909268164	22.524099543089033
50-51	21.32182170076295	27.28736856149176	28.532435173475907	22.85837456426939
52-53	22.768682680274154	28.34188444980465	25.402977168386343	23.486455701534855
54-55	22.14768962729216	26.590859170874243	26.44605224939526	24.81539895243834
56-57	23.97270219012857	25.573913988796647	26.369581843385788	24.083801977688992
58-59	25.29882762695525	25.92691275098216	26.04937856972562	22.724881052336972
60-61	23.39689494575596	26.756777219021	28.030560788343223	21.815767046879817
62-63	22.20869788036486	26.90213837646256	27.419332502136385	23.469831241036196
64-65	21.614107947475418	28.903239021340134	26.232469657887002	23.250183373297446
66-67	23.37542111555318	26.320493317216275	25.41434851317516	24.88973705405538
68-69	22.40356320547517	26.614104797282984	26.35289317639683	24.629438820845017
70-71	24.08877421178194	26.374978106896723	25.85852477635863	23.677722904962703
72-73	23.640589716115592	25.1596768424814	28.288373545585095	22.911359895817913
74-75	22.38284688990454	28.446798283819884	26.314576517895233	22.85577830838034
76-77	22.882857123191577	26.173005626519487	26.22612871427284	24.7180085360161
78-79	23.284047974355822	26.409473523757203	26.870878960937116	23.435599540949855
80-81	24.075185170288663	25.94053403845173	26.70680710984248	23.277473681417128
82-83	23.297016271977476	25.334880194168687	28.218160122985886	23.149943410867948
84-85	22.748290708649975	26.91185790345835	27.138138008141304	23.20171337975036
86-87	22.58030115051248	27.23113667123674	26.62337743110123	23.56518474714955
88-89	22.52336478048442	26.763058047616372	25.654082001419358	25.059495170479845
90-91	23.466624783313353	25.32776654033108	26.198941394927957	25.006667281427607
92-93	24.34229249090518	26.45147241835893	26.601493778906395	22.6047413118295
94-95	23.19969498252725	26.131466477805258	27.143470404623287	23.525368135044197
96-97	21.674541696168834	26.313513543992133	28.55736419253674	23.454580567302294
98-99	23.092323432682075	27.185119325389778	25.944242087289748	23.778315154638396
100	22.92828544966268	24.529750728520117	26.032668131015356	26.509295690801842
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1830.0
1	1132.0
2	410.0
3	425.5
4	416.0
5	349.5
6	301.0
7	313.5
8	352.5
9	361.0
10	347.5
11	325.0
12	347.0
13	381.5
14	431.0
15	483.5
16	562.5
17	713.5
18	961.0
19	1262.5
20	1669.0
21	2322.0
22	3472.0
23	5225.0
24	7805.0
25	11422.0
26	16383.5
27	22880.0
28	29850.5
29	38847.0
30	50174.0
31	61627.5
32	75049.0
33	89065.5
34	99622.0
35	110585.5
36	123347.5
37	137216.0
38	146566.5
39	149636.0
40	151616.0
41	154224.5
42	159833.0
43	169564.0
44	183194.5
45	201178.5
46	224997.0
47	306312.0
48	465612.0
49	472986.5
50	354516.5
51	286777.5
52	235687.5
53	211606.0
54	197144.5
55	177208.0
56	158550.5
57	139489.0
58	120440.0
59	105473.0
60	86995.5
61	68411.5
62	53400.5
63	41438.0
64	31137.0
65	21834.0
66	15607.5
67	11675.5
68	8552.5
69	6116.5
70	4722.0
71	3612.5
72	2696.5
73	2257.0
74	1889.0
75	1475.0
76	1105.5
77	775.0
78	478.5
79	281.0
80	166.0
81	96.5
82	72.0
83	69.0
84	68.0
85	58.0
86	46.0
87	41.0
88	39.0
89	31.5
90	22.0
91	15.0
92	16.0
93	12.5
94	12.5
95	16.0
96	13.5
97	8.0
98	9.0
99	12.0
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09789101853218812
2	0.06367555589356787
3	0.08031106412077114
4	0.08221652572050059
5	0.0821668180265946
6	0.09013661828285434
7	0.08614343353907349
8	0.08738612588672312
9	0.07293775619138324
10-11	0.08018679488600618
12-13	0.06881201759718641
14-15	0.07838074867408869
16-17	0.0797394256408523
18-19	0.07948260255567137
20-21	0.07093287920384181
22-23	0.0751497485701996
24-25	0.07707177940123107
26-27	0.07070919458126487
28-29	0.07943289486176539
30-31	0.08323553344557331
32-33	0.08448651040887395
34-35	0.08706302587633422
36-37	0.08662394124683134
38-39	0.0881648797579169
40-41	0.084320818095854
42-43	0.08932472594905658
44-45	0.08629255662079144
46-47	0.08258933342479549
48-49	0.09200065680432881
50-51	0.08822287206747388
52-53	0.09379841840059529
54-55	0.08590317968519455
56-57	0.09226576450516075
58-59	0.09685544157581343
60-61	0.094883703050876
62-63	0.09652405694977352
64-65	0.0980152877669531
66-67	0.10039297245878942
68-69	0.09743536467138326
70-71	0.09919998780504576
72-73	0.09675602618800146
74-75	0.10072435708482932
76-77	0.09758448775310122
78-79	0.10458498797819424
80-81	0.10000359552319253
82-83	0.10436130335561729
84-85	0.09991246475103156
86-87	0.09675602618800146
88-89	0.09613468001417665
90-91	0.09674774157235046
92-93	0.09071654137842416
94-95	0.09054256444975323
96-97	0.08878622593174172
98-99	0.10275408791932375
100	0.2462022079163479
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6035283.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.917682087366714
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.971343953596	37.5563901664955
2	14.713800131797333	15.572403953430115
3	5.964614771740296	9.469007647936929
4	2.992428024105299	6.334094193957247
5	1.6908413041071835	4.473770129546624
6	1.0358690136519446	3.2889472289152644
7	0.6542075581624516	2.4233403307395154
8	0.4705396513633565	1.9919894144277175
9	0.3095197636945146	1.4741161609448878
>10	1.1767403053985486	9.500359375357764
>50	0.01618299924687158	0.5652493368704427
>100	0.0033227352730793155	0.26697705469842503
>500	5.361707846190305E-5	0.021620161325252917
>1k	1.6085123538325048E-4	0.16121490373780753
>5k	0.0	0.0
>10k+	3.7531954922758446E-4	6.900519941616453
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	113070	1.8734829833166065	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	29317	0.4857601540805957	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	26267	0.4352239986095101	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	21775	0.3607950116009473	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	19372	0.32097914878225264	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	18825	0.3119157792600612	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	14582	0.2416125308456952	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6569231301995285E-5	0.0	0.0	0.0	0.0
2	1.6569231301995285E-5	0.0	0.0	0.0	0.0
3	3.313846260399057E-5	0.0	0.0	0.0	0.0
4	3.313846260399057E-5	0.0	0.0	0.0	0.0
5	6.627692520798114E-5	0.0	0.0	0.0	0.0
6	8.284615650997642E-5	0.0	0.0	0.0	0.0
7	8.284615650997642E-5	0.0	0.0	0.0	0.0
8	9.94153878119717E-5	0.0	0.0	0.0	0.0
9	9.94153878119717E-5	0.0	0.0	0.0	1.6569231301995285E-5
10-11	2.4853846952992923E-4	0.0	0.0	1.6569231301995285E-5	1.6569231301995285E-5
12-13	3.976615512478868E-4	0.0	0.0	4.142307825498821E-5	1.6569231301995285E-5
14-15	4.4736924515387266E-4	0.0	0.0	7.456154085897879E-5	4.142307825498821E-5
16-17	5.385000173148467E-4	0.0	0.0	1.2426923476496462E-4	6.627692520798114E-5
18-19	5.716384799188373E-4	0.0	0.0	2.3196923822793396E-4	6.627692520798114E-5
20-21	5.799230955698349E-4	0.0	0.0	4.39084629502875E-4	6.627692520798114E-5
22-23	7.787538711937783E-4	0.0	0.0	8.947384903077453E-4	9.941538781197171E-5
24-25	0.0010272923407237075	0.0	0.0	0.0016155000519445402	1.3255385041596228E-4
26-27	0.001176415422441665	0.0	0.0	0.0026925000865742335	1.408384660669599E-4
28-29	0.0012095538850456556	0.0	0.0	0.006420577129523172	1.4912308171795754E-4
30-31	0.001342107735461618	0.0	0.0	0.016502954376787303	1.4912308171795754E-4
32-33	0.0014001000450186013	0.0	0.0	0.04244208598006092	1.4912308171795754E-4
34-35	0.0016320692832465355	0.0	0.0	0.08066730259376403	1.4912308171795754E-4
36-37	0.0018557539058234717	0.0	0.0	0.13617422745544824	1.4912308171795754E-4
38-39	0.0018557539058234717	0.0	0.0	0.22124894557554303	1.6569231301995284E-4
40-41	0.0019220308310314528	0.0	0.0	0.319637041046791	1.6569231301995284E-4
42-43	0.0019220308310314528	0.0	0.0	0.4203365442846674	1.6569231301995284E-4
44-45	0.001963453909286441	0.0	0.0	0.5452188406078058	1.6569231301995284E-4
46-47	0.002013161603192427	0.0	0.0	0.699188422481597	1.6569231301995284E-4
48-49	0.0020214462188434244	0.0	0.0	0.8509957196704778	2.154000069259387E-4
50-51	0.0020214462188434244	0.0	0.0	1.0158430018940288	2.154000069259387E-4
52-53	0.0021622846849103845	0.0	0.0	1.1855036458108095	2.154000069259387E-4
54-55	0.002170569300561382	0.0	0.0	1.3750887903682396	2.154000069259387E-4
56-57	0.002170569300561382	0.0	0.0	1.6199654597804278	2.154000069259387E-4
58-59	0.002170569300561382	0.0	0.0	1.8702685524440197	2.154000069259387E-4
60-61	0.002203707763165373	0.0	0.0	2.12969300693936	2.154000069259387E-4
62-63	0.002220276994467368	0.0	0.0	2.400152569481829	2.154000069259387E-4
64-65	0.002220276994467368	0.0	0.0	2.6783251091953764	2.154000069259387E-4
66-67	0.002220276994467368	0.0	0.0	3.004183896596067	2.3196923822793396E-4
68-69	0.0023694000761853256	0.0	0.0	3.3820617856693715	2.4853846952992923E-4
70-71	0.002377684691836323	0.0	0.0	3.782490398544691	2.6510770083192455E-4
72-73	0.0023859693074873207	0.0	0.0	4.166904849366633	3.065307790869128E-4
74-75	0.0023859693074873207	0.0	0.0	4.5716746008430755	3.313846260399057E-4
76-77	0.0024356770013933067	0.0	0.0	4.996617391429698	3.313846260399057E-4
78-79	0.0024853846952992923	0.0	0.0	5.529989231656577	3.313846260399057E-4
80-81	0.0024853846952992923	0.0	0.0	6.088579441925093	3.313846260399057E-4
82-83	0.0025268077735542804	0.0	0.0	6.623972728370815	3.4795385734190094E-4
84-85	0.002568230851809269	0.0	0.0	7.157402560907252	3.562384729928986E-4
86-87	0.002568230851809269	0.0	0.0	7.701494693786522	3.810923199458915E-4
88	0.0025848000831112643	0.0	0.0	8.15481229297781	4.1423078254988207E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	2100	0.0	21.722927	1
CATGGGG	11235	0.0	20.126678	4
GTACGCA	1600	0.0	18.510914	3
GAGTACG	1810	0.0	17.928188	1
ATGGGGG	7850	0.0	17.785763	5
CTAGTAC	3190	0.0	17.537336	3
TAGTACT	3095	0.0	17.467764	4
GTATAAG	2710	0.0	17.35388	1
TACCTGG	5400	0.0	17.32488	2
TACGCAG	1690	0.0	17.246664	4
GGCGTCG	1695	0.0	17.189087	8
GTGATCG	1670	0.0	16.88362	8
CTGTGCG	3430	0.0	16.850788	9
ATACGGG	1760	0.0	16.828104	3
TGGGGGG	7560	0.0	16.224627	6
TGATCGC	1630	0.0	16.143942	9
ACGGGCG	1835	0.0	16.139503	5
CGACGAG	1050	0.0	16.111399	24-25
TCTAACG	1025	0.0	16.05296	2
TACGGGC	1820	0.0	16.01476	4
>>END_MODULE