FastQCFastQC Report
Mon 16 Jan 2017
ERR1146636_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146636_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6035283
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1085251.798175827049038No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC262430.43482633705826224No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC226240.3748622889763413No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA191220.31683684095675385No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA178250.2953465479580659No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA166390.27569543963389953No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC147660.24466126940526234No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG100440.16642135919724063No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79240.13129458883701062No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72660.12039203464029773No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG21950.024.2112081
GAGTACT111600.020.80402612-13
TGATCGC14200.020.1890249
GTACCCG7250.020.1092661
TACGCAG16000.019.1047234
GTACGCA15900.018.929113
GTGATCG15400.018.6161618
CGACGAG10100.018.61298624-25
GAGTACG17200.018.3197231
GTACTTT127100.018.26702514-15
AGTACTT124650.017.73993312-13
TAGTACT30550.017.7024754
CATGGGG104500.017.6857554
TACCTGG52350.017.5180972
GTATAAG27200.017.1174411
GTATTAG13200.016.7454381
CTAGTAC30250.016.6343483
ATGGGGG74300.016.5109585
CTGTGCG31100.016.3206399
TAACGCC10100.016.2964884
Produced by FastQC (version 0.11.5)