##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146636_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6035283
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.388915979582066	33.0	31.0	34.0	30.0	34.0
2	32.46255859087304	34.0	31.0	34.0	30.0	34.0
3	32.585079440351016	34.0	31.0	34.0	31.0	34.0
4	36.01702969023988	37.0	35.0	37.0	35.0	37.0
5	36.062209510307966	37.0	35.0	37.0	35.0	37.0
6	36.14703585565085	37.0	36.0	37.0	35.0	37.0
7	36.12683464884745	37.0	36.0	37.0	35.0	37.0
8	36.13809509844029	37.0	36.0	37.0	35.0	37.0
9	37.932539865984744	39.0	38.0	39.0	35.0	39.0
10-11	37.87956687366607	39.0	38.0	39.0	35.0	39.0
12-13	37.86089997768124	39.0	38.0	39.0	35.0	39.0
14-15	39.19976859411564	40.0	39.0	41.0	36.0	41.0
16-17	39.17883419882713	40.0	39.0	41.0	36.0	41.0
18-19	39.27223868375352	40.5	39.0	41.0	36.0	41.0
20-21	39.31928892149713	41.0	39.0	41.0	36.0	41.0
22-23	39.2853889370225	41.0	39.0	41.0	36.0	41.0
24-25	39.26324241961811	41.0	39.0	41.0	36.0	41.0
26-27	39.16835275164396	40.5	39.0	41.0	36.0	41.0
28-29	39.14228454572884	40.0	39.0	41.0	36.0	41.0
30-31	39.07599693336667	40.0	39.0	41.0	35.5	41.0
32-33	38.99400467219184	40.0	38.5	41.0	35.0	41.0
34-35	38.92184989502563	40.0	38.0	41.0	35.0	41.0
36-37	38.8606289879033	40.0	38.0	41.0	35.0	41.0
38-39	38.79368084313528	40.0	38.0	41.0	35.0	41.0
40-41	38.77344318402302	40.0	38.0	41.0	35.0	41.0
42-43	38.69944077187432	40.0	38.0	41.0	35.0	41.0
44-45	38.58772024443593	40.0	38.0	41.0	35.0	41.0
46-47	38.409137152309185	40.0	38.0	41.0	34.0	41.0
48-49	38.1722938261553	40.0	38.0	41.0	34.0	41.0
50-51	38.050909874483104	40.0	37.0	41.0	33.0	41.0
52-53	38.02264823372823	40.0	37.0	41.0	33.0	41.0
54-55	37.87853792440222	40.0	37.0	41.0	33.0	41.0
56-57	37.694161400550726	40.0	36.5	41.0	33.0	41.0
58-59	37.508720303588085	39.0	36.0	41.0	33.0	41.0
60-61	37.219148629815706	39.0	35.5	41.0	32.0	41.0
62-63	37.01813825466014	39.0	35.0	41.0	32.0	41.0
64-65	36.75001520226972	38.5	35.0	40.5	32.0	41.0
66-67	36.42833293815717	37.5	35.0	40.0	32.0	41.0
68-69	36.070323794261185	37.0	35.0	39.5	31.0	41.0
70-71	35.67354049511846	36.0	35.0	39.0	31.0	41.0
72-73	35.30422699316668	36.0	35.0	39.0	31.0	41.0
74-75	35.00528409355452	35.0	34.5	37.5	31.0	40.0
76-77	34.70805445245898	35.0	34.0	37.0	31.0	39.0
78-79	34.40805841250526	35.0	34.0	37.0	30.0	39.0
80-81	34.128982849685755	35.0	34.0	36.0	30.0	39.0
82-83	33.865801736223474	35.0	34.0	36.0	30.0	37.0
84-85	33.668859107352546	35.0	34.0	35.5	30.0	37.0
86-87	33.56002527139158	35.0	34.0	35.0	30.0	36.5
88-89	33.376019815475104	35.0	34.0	35.0	29.0	36.0
90-91	33.27202535490051	35.0	34.0	35.0	29.0	36.0
92-93	33.16139889380498	35.0	34.0	35.0	29.0	36.0
94-95	33.07636750422474	35.0	34.0	35.0	29.0	35.5
96-97	33.024834626644676	35.0	34.0	35.0	29.0	35.0
98-99	33.0029721058648	35.0	34.0	35.0	29.0	35.0
100	32.202365489737595	35.0	32.0	35.0	26.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.28341060565823994
1101	2	0.309531236235955
1101	3	0.2563307863292579
1101	4	0.18089551538780313
1101	5	0.18012304897959552
1101	6	0.2217109964744779
1101	7	0.2227661070967457
1101	8	0.1938052504753287
1101	9	0.2549357602673794
1101	10-11	0.25463626780075543
1101	12-13	0.2638648049030934
1101	14-15	0.39378022977725635
1101	16-17	0.42154027866366306
1101	18-19	0.3919403306795388
1101	20-21	0.3631262085899394
1101	22-23	0.32254636777499
1101	24-25	0.29556466333218623
1101	26-27	0.33194142748376265
1101	28-29	0.3718848918673956
1101	30-31	0.40122986481208045
1101	32-33	0.35994136328306325
1101	34-35	0.3677060580841385
1101	36-37	0.3484070389685954
1101	38-39	0.3509672220108939
1101	40-41	0.373154172669075
1101	42-43	0.3827397296371444
1101	44-45	0.38907810195760817
1101	46-47	0.4189517996050327
1101	48-49	0.4508979105632349
1101	50-51	0.4456397615833936
1101	52-53	0.4096766731012451
1101	54-55	0.3993425002304889
1101	56-57	0.40400593109040983
1101	58-59	0.3919474526167832
1101	60-61	0.43296259516262126
1101	62-63	0.4243533876741665
1101	64-65	0.3814076302245297
1101	66-67	0.31090730936966793
1101	68-69	0.32419884073375016
1101	70-71	0.29768694562616815
1101	72-73	0.28974814092858026
1101	74-75	0.29853733166685004
1101	76-77	0.2985650236219044
1101	78-79	0.28322187142968147
1101	80-81	0.24954693577202391
1101	82-83	0.30094811672012156
1101	84-85	0.23206702084418396
1101	86-87	0.2617337205282553
1101	88-89	0.36051300738165537
1101	90-91	0.3260210218902273
1101	92-93	0.37049697586100194
1101	94-95	0.3538603792038373
1101	96-97	0.2944203899289164
1101	98-99	0.3208203158377856
1101	100	0.31795957383729245
1102	1	0.27498597554874493
1102	2	0.2525995278869999
1102	3	0.21767911644760574
1102	4	0.13929041635999795
1102	5	0.12990306665291484
1102	6	0.21292585269426922
1102	7	0.19003222016408472
1102	8	0.21531402334597516
1102	9	0.25722972319655923
1102	10-11	0.2639386618288455
1102	12-13	0.2817125767890687
1102	14-15	0.41625935254334934
1102	16-17	0.4881950682676788
1102	18-19	0.40673722118219047
1102	20-21	0.37460160282346777
1102	22-23	0.4034054878581941
1102	24-25	0.38420295979241814
1102	26-27	0.3507969940634226
1102	28-29	0.36755214175568796
1102	30-31	0.41889116512061264
1102	32-33	0.40030203838072964
1102	34-35	0.39985180175972346
1102	36-37	0.4031786554050356
1102	38-39	0.40889242919240587
1102	40-41	0.41723439044927346
1102	42-43	0.40642366223838877
1102	44-45	0.44775869345096453
1102	46-47	0.4766257592259322
1102	48-49	0.5463974190904821
1102	50-51	0.5120209266466276
1102	52-53	0.4546911542320373
1102	54-55	0.4349619818131245
1102	56-57	0.4194691767930152
1102	58-59	0.44494909878078914
1102	60-61	0.4065718590057017
1102	62-63	0.4178934955089275
1102	64-65	0.4117907616477936
1102	66-67	0.3818731365745123
1102	68-69	0.4014049530068675
1102	70-71	0.4090725954661849
1102	72-73	0.4212849063265196
1102	74-75	0.4360951952151879
1102	76-77	0.38148950965882733
1102	78-79	0.3940074957384354
1102	80-81	0.37267296628570534
1102	82-83	0.3815873854161751
1102	84-85	0.39575129857858826
1102	86-87	0.3494102270208188
1102	88-89	0.4198988841574618
1102	90-91	0.39965538703751946
1102	92-93	0.3886212255970065
1102	94-95	0.4360869477500273
1102	96-97	0.461656119161276
1102	98-99	0.39389696651237216
1102	100	0.41956728462024273
1103	1	0.2489797420661759
1103	2	0.2507078538529868
1103	3	0.2617753323903784
1103	4	0.20938075103442344
1103	5	0.1758170861972559
1103	6	0.1960984636653933
1103	7	0.1719966063421623
1103	8	0.171817635314639
1103	9	0.2196633580000551
1103	10-11	0.24176054189636176
1103	12-13	0.2721569931299115
1103	14-15	0.364332427538983
1103	16-17	0.42258999851035384
1103	18-19	0.36024450067008473
1103	20-21	0.3110207306559545
1103	22-23	0.36587738754569443
1103	24-25	0.3395311454400485
1103	26-27	0.33894197158317496
1103	28-29	0.29703734757644185
1103	30-31	0.36052554548360405
1103	32-33	0.3266857202249085
1103	34-35	0.3097982781991959
1103	36-37	0.3322005458735049
1103	38-39	0.33344501075718824
1103	40-41	0.33619704865019173
1103	42-43	0.29660758188627057
1103	44-45	0.34626559472224727
1103	46-47	0.4011595777008736
1103	48-49	0.4731302125983987
1103	50-51	0.3835658587803863
1103	52-53	0.33445387649339153
1103	54-55	0.36689096927997156
1103	56-57	0.318830758967664
1103	58-59	0.32128319879071654
1103	60-61	0.2794451292556559
1103	62-63	0.331885560051596
1103	64-65	0.3346949983830285
1103	66-67	0.3058699170055803
1103	68-69	0.30079401498237957
1103	70-71	0.35418257967563704
1103	72-73	0.28816063616638843
1103	74-75	0.29449473615477473
1103	76-77	0.2914074721113735
1103	78-79	0.30198488193069295
1103	80-81	0.28105885596936986
1103	82-83	0.30024747032580734
1103	84-85	0.29136306986154636
1103	86-87	0.27991573125448355
1103	88-89	0.35792853515808787
1103	90-91	0.33210708168433456
1103	92-93	0.35487174564485713
1103	94-95	0.3441197367666149
1103	96-97	0.34671490820623774
1103	98-99	0.2808780119503709
1103	100	0.2923434891219614
1104	1	0.22354939604744573
1104	2	0.21318299008980546
1104	3	0.19177960613790646
1104	4	0.1317323517890543
1104	5	0.14624600785307962
1104	6	0.15730007320864559
1104	7	0.14926940398854782
1104	8	0.18066205930939105
1104	9	0.2281684746383803
1104	10-11	0.2286207694017648
1104	12-13	0.20964606499870797
1104	14-15	0.32557788137051347
1104	16-17	0.38370165479273766
1104	18-19	0.298898048506409
1104	20-21	0.3059579424069341
1104	22-23	0.3235147628019348
1104	24-25	0.30216602015540417
1104	26-27	0.29931185728364795
1104	28-29	0.34214555205170427
1104	30-31	0.36576472381727143
1104	32-33	0.30530547798307595
1104	34-35	0.29098000332007246
1104	36-37	0.2808450409019372
1104	38-39	0.2979337725206932
1104	40-41	0.28301238698315956
1104	42-43	0.283768781819127
1104	44-45	0.29731109982294157
1104	46-47	0.32574976536891853
1104	48-49	0.39595978527107434
1104	50-51	0.33385529696474947
1104	52-53	0.27231367306643506
1104	54-55	0.36074917836695874
1104	56-57	0.32591450208533246
1104	58-59	0.30891433307111527
1104	60-61	0.23416338408036808
1104	62-63	0.26257898958806436
1104	64-65	0.24081405845372217
1104	66-67	0.23807518390695037
1104	68-69	0.22921113208240484
1104	70-71	0.21396068113033095
1104	72-73	0.19864128057663066
1104	74-75	0.2617607091132257
1104	76-77	0.20087187443630938
1104	78-79	0.18977637891897814
1104	80-81	0.20144651425351157
1104	82-83	0.17391112540703801
1104	84-85	0.1932789829071595
1104	86-87	0.19967580022210996
1104	88-89	0.23435158042752846
1104	90-91	0.2083921294446256
1104	92-93	0.2631278853604897
1104	94-95	0.25106410544707103
1104	96-97	0.27633661500937023
1104	98-99	0.21719516321665822
1104	100	0.2913386281047323
1105	1	0.1666741471489388
1105	2	0.12192375786155196
1105	3	0.1324719759262294
1105	4	0.12368323378694868
1105	5	0.07202844520634244
1105	6	0.09810812781770295
1105	7	0.0964221687262139
1105	8	0.12245645770037328
1105	9	0.15047750365908996
1105	10-11	0.1328051007314599
1105	12-13	0.15943242081731057
1105	14-15	0.20919145152272023
1105	16-17	0.311440688383108
1105	18-19	0.2629561156498781
1105	20-21	0.2137856864815646
1105	22-23	0.2055661560449238
1105	24-25	0.1843237343397135
1105	26-27	0.18147223062788242
1105	28-29	0.21003360041877528
1105	30-31	0.21025302849098892
1105	32-33	0.22329862225968355
1105	34-35	0.19837300883854425
1105	36-37	0.15303108718523362
1105	38-39	0.14482086672664707
1105	40-41	0.1572667824040579
1105	42-43	0.21557814832586075
1105	44-45	0.19754114670279677
1105	46-47	0.21975490068401626
1105	48-49	0.3414828486196768
1105	50-51	0.2651061489755051
1105	52-53	0.12753364263927836
1105	54-55	0.18018660558382038
1105	56-57	0.18130272602513742
1105	58-59	0.1383657247413268
1105	60-61	0.16770497736445833
1105	62-63	0.15624291150167124
1105	64-65	0.169161289647775
1105	66-67	0.17255871072939755
1105	68-69	0.1478410428860002
1105	70-71	0.15316364862553655
1105	72-73	0.17027281701332697
1105	74-75	0.18422256028217987
1105	76-77	0.140704903739028
1105	78-79	0.12952799701778872
1105	80-81	0.16662276950995647
1105	82-83	0.14964974571395828
1105	84-85	0.13346152128121958
1105	86-87	0.16119786972673467
1105	88-89	0.2015730705852974
1105	90-91	0.1481913169599025
1105	92-93	0.15833355183483633
1105	94-95	0.1589699197297989
1105	96-97	0.15650698215844017
1105	98-99	0.17051045153618105
1105	100	0.11404884481081012
1106	1	0.0795960642796274
1106	2	0.08670398400089852
1106	3	0.09534545981848197
1106	4	0.1141244677713189
1106	5	0.0880150950282399
1106	6	0.09239838979402748
1106	7	0.08614318223659012
1106	8	0.11775303144982274
1106	9	0.1279694792498276
1106	10-11	0.12577673063768913
1106	12-13	0.1461932026895454
1106	14-15	0.22678373959512044
1106	16-17	0.2899295171712666
1106	18-19	0.19636392464133934
1106	20-21	0.18682397679869212
1106	22-23	0.16450552078276814
1106	24-25	0.1797837980935384
1106	26-27	0.19482880569159278
1106	28-29	0.19077223752489658
1106	30-31	0.17746727105149063
1106	32-33	0.1976873277665092
1106	34-35	0.19112456297330027
1106	36-37	0.20496499276167413
1106	38-39	0.2154564943963564
1106	40-41	0.17887045747782082
1106	42-43	0.16664225106008246
1106	44-45	0.16456355234694797
1106	46-47	0.18449140702444566
1106	48-49	0.24823388434091953
1106	50-51	0.14889626524077926
1106	52-53	0.18787588344402195
1106	54-55	0.23687527161531818
1106	56-57	0.2134113920788252
1106	58-59	0.2528703418157576
1106	60-61	0.19132939465531962
1106	62-63	0.18774511212251355
1106	64-65	0.14131309602754527
1106	66-67	0.17075667556375151
1106	68-69	0.21890907802487192
1106	70-71	0.1826798220912309
1106	72-73	0.17930373255173748
1106	74-75	0.20021431038709636
1106	76-77	0.1469737422790942
1106	78-79	0.15060315166190463
1106	80-81	0.17135725850114625
1106	82-83	0.15044180237758553
1106	84-85	0.1157066220667744
1106	86-87	0.12317150665196408
1106	88-89	0.2059654876285748
1106	90-91	0.20123882034759788
1106	92-93	0.18247445202297996
1106	94-95	0.1963560243948379
1106	96-97	0.17681234705249693
1106	98-99	0.18719411842587164
1106	100	0.15513372802866598
1107	1	0.10446783385889091
1107	2	0.05753528673212571
1107	3	0.07406934806586207
1107	4	0.044336118636458366
1107	5	0.0606462545620019
1107	6	0.07079120986711018
1107	7	0.0657875694136223
1107	8	0.07265205640270977
1107	9	0.06006155990701956
1107	10-11	0.09709131531458581
1107	12-13	0.12007110037367852
1107	14-15	0.1859901165375959
1107	16-17	0.25931733580488725
1107	18-19	0.158336980002737
1107	20-21	0.1418663172264658
1107	22-23	0.16426438508667474
1107	24-25	0.13303487481454113
1107	26-27	0.14635339586264706
1107	28-29	0.18493702361256226
1107	30-31	0.14393930375989328
1107	32-33	0.12460679205216252
1107	34-35	0.10318886385421422
1107	36-37	0.11686725240810603
1107	38-39	0.09127709095768921
1107	40-41	0.08367386372401597
1107	42-43	0.09564907800677958
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2216	56-57	-0.6941376886551041
2216	58-59	-0.7569084739318086
2216	60-61	-0.7266541562856048
2216	62-63	-0.7288338664914562
2216	64-65	-0.6349620501091238
2216	66-67	-0.6611280419689365
2216	68-69	-0.6059147499143762
2216	70-71	-0.6146136711462304
2216	72-73	-0.5886574536946299
2216	74-75	-0.5736081516267575
2216	76-77	-0.5575253650404761
2216	78-79	-0.5580584674007625
2216	80-81	-0.5892509280426168
2216	82-83	-0.6002848965982679
2216	84-85	-0.6091568193228198
2216	86-87	-0.5621774744665089
2216	88-89	-0.5267993602764562
2216	90-91	-0.643827018547519
2216	92-93	-0.6358518035433889
2216	94-95	-0.6209064065936332
2216	96-97	-0.6221913130949588
2216	98-99	-0.6421473108737104
2216	100	-0.6966614593832361
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	30.0
8	173.0
9	283.0
10	277.0
11	190.0
12	188.0
13	248.0
14	419.0
15	799.0
16	1412.0
17	2615.0
18	4512.0
19	7034.0
20	10084.0
21	14675.0
22	21590.0
23	26337.0
24	27068.0
25	27575.0
26	30042.0
27	36772.0
28	46896.0
29	59656.0
30	75013.0
31	94889.0
32	121327.0
33	159515.0
34	230280.0
35	356164.0
36	671160.0
37	1502108.0
38	1930322.0
39	574282.0
40	1348.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.07602303716348	19.061201454553167	13.265241872652696	23.597533635630654
2	14.611767279090113	22.05163511758	37.76255004109335	25.57404756223654
3	21.51025786070359	24.957690404776983	29.830774874512738	23.701276860006686
4	12.226698164034978	17.128998300799868	39.96221973558789	30.68208379957727
5	12.572121562394006	38.941294154236786	35.136980638174236	13.349603645194968
6	29.558036820184903	37.65131926154162	17.72444264752059	15.066201270752883
7	26.019235889762214	32.63891109626876	22.949933897029815	18.391919116939214
8	25.157623698067688	32.196226840268686	20.68283719342663	21.963312268236994
9	24.700730488184554	17.404315525429727	22.287917426849695	35.60703655953602
10-11	25.093148106939843	24.98082525072155	28.233714387118013	21.692312255220596
12-13	27.598390995086724	23.161995883208792	27.211921959583336	22.027691162121148
14-15	23.473900927404962	24.82088863626328	27.53465600568921	24.170554430642547
16-17	21.760697871477415	27.612403496382758	27.82571638101784	22.801182251121986
18-19	21.380788092942858	29.167223117145557	27.951388486164184	21.5006003037474
20-21	23.20587917553951	26.301698615537866	26.86187257964352	23.630549629279095
22-23	22.08218736387341	26.521498660460495	27.295836831512293	24.100477144153803
24-25	23.872194559890563	26.43881819626354	26.87341256408357	22.815574679762324
26-27	23.35035881593971	25.05203995117366	29.177507805371327	22.420093427515305
28-29	21.789843457190567	28.521484165942866	27.548339911871555	22.14033246499501
30-31	22.87231887668388	26.096572094040145	27.181369659552296	23.849739369723675
32-33	22.896248678083715	26.608616263805818	27.725964320583703	22.769170737526764
34-35	24.00309215430052	26.115133619854408	27.675244928793212	22.20652929705186
36-37	23.36899862143927	25.310317002690848	29.092320488858842	22.228363887011042
38-39	22.836211993766284	26.697680052180168	28.350836217295956	22.115271736757595
40-41	22.610769267476662	27.11460241567538	27.646709795521247	22.627918521326713
42-43	22.366258549930468	27.00474857599884	26.2251082509304	24.40388462314029
44-45	23.30091601808839	25.575526920831145	26.875124684105984	24.24843237697448
46-47	24.292415119556114	26.758513229619886	27.208467274856872	21.740604375967127
48-49	23.909538542345402	26.304664532823885	27.344351606036344	22.441445318794372
50-51	21.245548883126112	26.928654381244428	28.990181570607376	22.835615165022087
52-53	22.616688172221174	27.944700002692507	25.890290604592593	23.548321220493722
54-55	22.07776735936846	26.270563125977898	26.93144633277423	24.720223181879415
56-57	23.829199413050482	25.212674756001057	26.816339015043567	24.1417868159049
58-59	24.96226328183355	25.629950510634547	26.621063368524446	22.786722839007457
60-61	23.249722794969017	26.444713751424135	28.452925846908595	21.852637606698256
62-63	22.0538978282831	26.591183726463978	27.874638935020485	23.48027951023243
64-65	21.40601410759554	28.50425072857265	26.745287423018016	23.344447740813795
66-67	23.080185982021824	26.107364668684934	25.89900396125601	24.91344538803723
68-69	22.15866838284126	26.291836726578126	26.82451805496089	24.724976835619724
70-71	23.76602725684812	26.058254000512505	26.27194661603373	23.903772126605645
72-73	23.309408568988797	24.804247220779043	28.69047975099665	23.19586445923551
74-75	22.110991818638915	27.987353359390255	26.719963586476858	23.18169123549397
76-77	22.509534325893004	25.869820826255218	26.59531774126948	25.025327106582296
78-79	22.82960085399028	26.115756775151105	27.2499035864195	23.804738784439117
80-81	23.561034512040795	25.71167129574973	26.945670927080034	23.78162326512944
82-83	22.77542663371466	25.101166208510463	28.489663586784392	23.63374357099049
84-85	22.333314996604944	26.501454953120383	27.33541433108112	23.82981571919355
86-87	22.061591809365027	26.940302550849726	26.91398232692651	24.084123312858736
88-89	21.927339612740614	26.49559266731983	25.935436664693274	25.64163105524629
90-91	22.646147881230082	25.101070248161538	26.50281904782419	25.749962822784184
92-93	23.4954935370401	26.259592249004665	26.77825117043105	23.466663043524186
94-95	22.406433504327886	25.838270966715708	27.345566402884376	24.40972912607203
96-97	20.786696649349825	26.029104124154358	28.81814648793594	24.36605273855988
98-99	22.115823779855802	26.848959215731565	26.175792492163374	24.859424512249266
100	21.903059834504855	24.254839903023687	26.43791793095089	27.40418233152057
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	117.0
1	79.0
2	127.5
3	224.5
4	161.0
5	124.0
6	244.5
7	551.5
8	990.5
9	1236.0
10	1075.5
11	912.0
12	1016.0
13	1162.5
14	1419.5
15	1722.5
16	2062.0
17	2480.5
18	3016.5
19	3622.0
20	4216.0
21	5126.5
22	6449.5
23	8160.0
24	10667.5
25	14151.0
26	19106.5
27	25492.5
28	31657.5
29	39856.5
30	50729.0
31	61195.5
32	73993.0
33	87083.0
34	97335.0
35	108452.5
36	120768.5
37	134742.0
38	143845.0
39	146450.0
40	149249.0
41	151836.0
42	156874.5
43	167022.5
44	180472.0
45	198271.0
46	219932.0
47	306059.0
48	473446.5
49	474617.5
50	353818.0
51	287422.5
52	233973.5
53	210081.0
54	195735.0
55	176950.5
56	158798.0
57	140860.0
58	122128.0
59	106882.5
60	87885.0
61	68610.5
62	53324.5
63	40754.5
64	30071.5
65	21275.5
66	15202.5
67	11037.5
68	8002.0
69	5649.0
70	4241.0
71	3214.5
72	2333.0
73	2013.5
74	1732.5
75	1374.5
76	962.5
77	627.0
78	355.5
79	146.0
80	74.0
81	43.5
82	41.0
83	29.5
84	21.5
85	15.5
86	9.5
87	10.0
88	10.0
89	11.0
90	7.5
91	1.0
92	1.5
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0027339231648292215
2	0.004026323206384854
3	1.4912308171795754E-4
4	0.050254478538951694
5	0.0026510770083192454
6	0.004606246301954688
7	3.313846260399057E-5
8	3.810923199458915E-4
9	6.627692520798114E-5
10-11	3.313846260399057E-5
12-13	0.0
14-15	2.4853846952992925E-5
16-17	2.816769321339198E-4
18-19	8.284615650997642E-5
20-21	2.4853846952992923E-4
22-23	0.0
24-25	0.0
26-27	2.6510770083192455E-4
28-29	1.6569231301995285E-5
30-31	1.5740769736895518E-4
32-33	1.7397692867095047E-4
34-35	1.408384660669599E-4
36-37	4.970769390598585E-5
38-39	6.544846364288137E-4
40-41	5.799230955698349E-4
42-43	0.0
44-45	5.136461703618537E-4
46-47	0.0
48-49	9.113077216097407E-5
50-51	0.0
52-53	3.396692416909033E-4
54-55	4.3080001385187734E-4
56-57	1.8226154432194813E-4
58-59	7.787538711937784E-4
60-61	6.462000207778161E-4
62-63	1.988307756239434E-4
64-65	3.893769355968892E-4
66-67	0.0012592615789516414
68-69	0.002568230851809269
70-71	0.0035209616516739977
72-73	0.0030404539439161343
74-75	0.0026925000865742335
76-77	0.004092600131592835
78-79	0.0014166692763205966
80-81	0.0011681308067906676
82-83	6.876230990328043E-4
84-85	6.130615581738256E-4
86-87	0.0
88-89	0.0
90-91	8.284615650997642E-6
92-93	1.0770000346296933E-4
94-95	4.970769390598585E-5
96-97	3.562384729928986E-4
98-99	4.142307825498821E-5
100	0.07509175626064263
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6035283.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.000133410500084
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.35802119918813	35.37268334246889
2	15.443497677387812	15.752408837430535
3	6.266330313288999	9.587510459159994
4	3.205550099922961	6.5393393100045145
5	1.8086329897000029	4.612026188266589
6	1.1120353411781083	3.40283704543647
7	0.7040233251017136	2.5133698453003888
8	0.4766211917247269	1.9446195491386087
9	0.3481079266637687	1.5978195630994305
>10	1.2489844378135182	9.73135328112518
>50	0.020855736232174356	0.706083811955523
>100	0.005840747417310919	0.527622874761096
>500	6.107095648748871E-4	0.21856186566014887
>1k	3.3311430594998325E-4	0.3873746430022866
>5k	1.1103810198332776E-4	0.4301016612239407
>10k+	4.4415240793331106E-4	6.67628772196646
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	108525	1.798175827049038	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	26243	0.43482633705826224	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	22624	0.3748622889763413	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	19122	0.31683684095675385	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	17825	0.2953465479580659	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	16639	0.27569543963389953	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	14766	0.24466126940526234	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	10044	0.16642135919724063	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7924	0.13129458883701062	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7266	0.12039203464029773	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	8.284615650997642E-5	0.0	0.0	0.0	0.0
6	8.284615650997642E-5	0.0	0.0	0.0	0.0
7	8.284615650997642E-5	0.0	0.0	0.0	0.0
8	8.284615650997642E-5	0.0	0.0	0.0	0.0
9	8.284615650997642E-5	0.0	0.0	0.0	0.0
10-11	1.988307756239434E-4	0.0	0.0	1.6569231301995285E-5	0.0
12-13	2.3196923822793396E-4	0.0	0.0	4.142307825498821E-5	2.485384695299293E-5
14-15	2.4853846952992923E-4	0.0	0.0	7.456154085897879E-5	5.799230955698349E-5
16-17	3.148153947379104E-4	0.0	0.0	1.2426923476496462E-4	8.284615650997642E-5
18-19	3.645230886438962E-4	0.0	0.0	2.154000069259387E-4	8.284615650997642E-5
20-21	3.7280770429489385E-4	0.0	0.0	4.308000138518774E-4	9.113077216097405E-5
22-23	4.8050770775786325E-4	0.0	0.0	8.7816925900575E-4	1.1598461911396698E-4
24-25	7.456154085897877E-4	0.0	0.0	0.0016155000519445402	1.3255385041596225E-4
26-27	9.113077216097406E-4	0.0	0.0	0.0027173539335272265	1.4912308171795754E-4
28-29	9.113077216097406E-4	0.0	0.0	0.006478569439080156	1.4912308171795754E-4
30-31	0.001043861572025703	0.0	0.0	0.016527808223740295	1.4912308171795754E-4
32-33	0.0011432769598376745	0.0	0.0	0.042500078289617896	1.4912308171795754E-4
34-35	0.001342107735461618	0.0	0.0	0.08072529490332103	1.6569231301995284E-4
36-37	0.0015575077423875567	0.0	0.0	0.13621565053370321	1.9054615997294577E-4
38-39	0.001574076973689552	0.0	0.0	0.22124894557554303	2.154000069259387E-4
40-41	0.0017397692867095048	0.0	0.0	0.31958733335288503	2.154000069259387E-4
42-43	0.0017397692867095048	0.0	0.0	0.4201377135090434	2.2368462257693633E-4
44-45	0.001806046211917486	0.0	0.0	0.5452022713765038	2.3196923822793396E-4
46-47	0.0018806077527764647	0.0	0.0	0.6987079147738391	2.3196923822793396E-4
48-49	0.0018888923684274622	0.0	0.0	0.8506809042757398	2.4853846952992923E-4
50-51	0.0018888923684274622	0.0	0.0	1.0156276018871029	2.6510770083192455E-4
52-53	0.0019220308310314528	0.0	0.0	1.1858847381307553	2.733923164829222E-4
54-55	0.0019386000623334483	0.0	0.0	1.3762652057906812	2.982461634359151E-4
56-57	0.0019386000623334483	0.0	0.0	1.6227656598704652	2.982461634359151E-4
58-59	0.0019386000623334483	0.0	0.0	1.8745019910416794	2.982461634359151E-4
60-61	0.0020214462188434244	0.0	0.0	2.135185707115971	2.982461634359151E-4
62-63	0.0020214462188434244	0.0	0.0	2.406788546618278	3.148153947379104E-4
64-65	0.0020214462188434244	0.0	0.0	2.6872642094828034	3.148153947379104E-4
66-67	0.0020214462188434244	0.0	0.0	3.0154261200344705	3.2310001038890804E-4
68-69	0.002087723144051406	0.0	0.0	3.3960296476569534	3.313846260399057E-4
70-71	0.002112576991004399	0.0	0.0	3.7995898452483505	3.313846260399057E-4
72-73	0.0021208616066553964	0.0	0.0	4.186887342316839	3.4795385734190094E-4
74-75	0.0021208616066553964	0.0	0.0	4.594440724652017	3.4795385734190094E-4
76-77	0.002154000069259387	0.0	0.0	5.021537515307898	3.4795385734190094E-4
78-79	0.002220276994467368	0.0	0.0	5.55642544019891	3.4795385734190094E-4
80-81	0.002220276994467368	0.0	0.0	6.115802688954271	3.562384729928986E-4
82-83	0.0022534154570713586	0.0	0.0	6.652587790829362	3.8109231994589153E-4
84-85	0.002286553919675349	0.0	0.0	7.186580977230065	3.8109231994589153E-4
86-87	0.002286553919675349	0.0	0.0	7.731559563983993	3.8109231994589153E-4
88	0.0023031231509773446	0.0	0.0	8.184984863178745	3.976615512478868E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	2195	0.0	24.211208	1
GAGTACT	11160	0.0	20.804026	12-13
TGATCGC	1420	0.0	20.189024	9
GTACCCG	725	0.0	20.109266	1
TACGCAG	1600	0.0	19.104723	4
GTACGCA	1590	0.0	18.92911	3
GTGATCG	1540	0.0	18.616161	8
CGACGAG	1010	0.0	18.612986	24-25
GAGTACG	1720	0.0	18.319723	1
GTACTTT	12710	0.0	18.267025	14-15
AGTACTT	12465	0.0	17.739933	12-13
TAGTACT	3055	0.0	17.702475	4
CATGGGG	10450	0.0	17.685755	4
TACCTGG	5235	0.0	17.518097	2
GTATAAG	2720	0.0	17.117441	1
GTATTAG	1320	0.0	16.745438	1
CTAGTAC	3025	0.0	16.634348	3
ATGGGGG	7430	0.0	16.510958	5
CTGTGCG	3110	0.0	16.320639	9
TAACGCC	1010	0.0	16.296488	4
>>END_MODULE