FastQCFastQC Report
Mon 16 Jan 2017
ERR1146635_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146635_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7620349
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA159360.20912428026590382No Hit
CATGTAAGCAGTGGTATCAACGCAGAGTACATGTAAGCAGTGGTATCAAC78320.10277744497004009No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG29450.023.6346781
CATGGGG164150.021.622754
TATTCGC6650.020.4915969
TAGTACT46750.020.4135864
CTAGTAC40350.019.9233843
TACCTGG60100.018.773592
ATGGGGG113500.018.5965565
CTGTGCG45650.018.5281129
ACATGGG412050.018.4603183
TACATGG437500.018.2998352
TAGGCAT42550.017.4557915
TACTGTG68150.017.3773127
CTCGTCG21450.017.30619
GCTATTG49500.017.0884958
CGACGAG16550.016.89556124-25
GCATCGT34700.016.7930188
CTAGGCA46850.016.7575974
CTATTGA53250.016.6779129
TCGCCGT18700.016.61196994
TGGGGGG101900.016.3260026
Produced by FastQC (version 0.11.5)