##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146635_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7620349
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.345540210822364	31.0	31.0	34.0	28.0	34.0
2	31.4215194081006	31.0	31.0	34.0	28.0	34.0
3	31.57896088486236	33.0	31.0	34.0	28.0	34.0
4	35.05434987295201	37.0	35.0	37.0	32.0	37.0
5	35.11707442795599	37.0	35.0	37.0	32.0	37.0
6	35.12380285994775	37.0	35.0	37.0	32.0	37.0
7	35.08846261503246	37.0	35.0	37.0	32.0	37.0
8	35.096669850685316	37.0	35.0	37.0	32.0	37.0
9	36.711396157840014	39.0	37.0	39.0	32.0	39.0
10-11	36.69638575608545	39.0	37.0	39.0	32.0	39.0
12-13	36.65322073831527	39.0	37.0	39.0	32.0	39.0
14-15	37.98254259745846	40.0	38.0	41.0	33.0	41.0
16-17	37.99491007564089	40.0	38.0	41.0	33.0	41.0
18-19	38.002850656839996	40.0	38.0	41.0	32.0	41.0
20-21	38.09319258212452	40.0	38.0	41.0	33.0	41.0
22-23	38.089274257648825	40.0	38.0	41.0	32.0	41.0
24-25	38.098004107160975	40.0	38.0	41.0	32.0	41.0
26-27	38.14060412456175	40.0	38.0	41.0	32.5	41.0
28-29	38.10968992365048	40.0	38.0	41.0	32.5	41.0
30-31	38.0723890729939	40.0	38.0	41.0	32.0	41.0
32-33	38.030837957684085	40.0	38.0	41.0	32.0	41.0
34-35	37.98144638782292	40.0	38.0	41.0	32.0	41.0
36-37	37.916684196484965	40.0	38.0	41.0	32.0	41.0
38-39	37.860533159308055	40.0	38.0	41.0	32.0	41.0
40-41	37.811560730355	40.0	38.0	41.0	32.0	41.0
42-43	37.65663888884879	40.0	37.5	41.0	31.0	41.0
44-45	37.52508618699747	40.0	37.0	41.0	31.0	41.0
46-47	37.409433281861496	40.0	37.0	41.0	31.0	41.0
48-49	37.30110615668653	40.0	37.0	41.0	30.0	41.0
50-51	37.17442403228513	40.0	36.0	41.0	30.0	41.0
52-53	37.06463339146278	40.0	36.0	41.0	30.0	41.0
54-55	36.95015733531365	39.0	35.5	41.0	30.0	41.0
56-57	36.75028158159161	39.0	35.0	41.0	30.0	41.0
58-59	36.56568098127789	39.0	35.0	41.0	30.0	41.0
60-61	36.33360965488589	39.0	35.0	40.5	29.5	41.0
62-63	36.09508796775581	38.0	35.0	40.0	29.0	41.0
64-65	35.82093904098093	37.5	35.0	40.0	29.0	41.0
66-67	35.57438917823842	37.0	34.5	40.0	29.0	41.0
68-69	35.30528877351943	36.5	34.0	39.5	29.0	41.0
70-71	34.99855977724904	36.0	34.0	39.0	28.5	41.0
72-73	34.651682226102764	35.5	34.0	39.0	28.0	41.0
74-75	34.31446145051886	35.0	34.0	38.5	28.0	40.0
76-77	34.02490312451569	35.0	34.0	37.0	28.0	39.5
78-79	33.71010540330896	35.0	33.5	37.0	27.0	39.0
80-81	33.464376106658634	35.0	33.0	36.0	27.0	39.0
82-83	33.204308818401884	35.0	33.0	36.0	27.0	37.5
84-85	33.00550978701894	35.0	33.0	36.0	27.0	37.0
86-87	32.81318060367052	35.0	33.0	35.0	26.0	37.0
88-89	32.64073935458862	35.0	33.0	35.0	26.0	36.0
90-91	32.50430118095641	35.0	33.0	35.0	26.0	36.0
92-93	32.35763552299245	35.0	33.0	35.0	26.0	36.0
94-95	32.25850449894093	35.0	33.0	35.0	25.0	36.0
96-97	32.18803548236439	35.0	33.0	35.0	25.0	35.0
98-99	32.11331797270702	35.0	33.0	35.0	25.0	35.0
100	30.931974637907004	34.0	30.0	35.0	21.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2871319795771292
1101	2	0.29141974727920683
1101	3	0.30222681466512924
1101	4	0.30774696595437945
1101	5	0.2501784516583072
1101	6	0.2950351887375078
1101	7	0.3203844716612281
1101	8	0.3213665803951784
1101	9	0.4159456135894075
1101	10-11	0.4883182234923069
1101	12-13	0.4652659285547429
1101	14-15	0.557931145615548
1101	16-17	0.565399608112898
1101	18-19	0.5330230079416296
1101	20-21	0.5157312323740655
1101	22-23	0.5254462811531013
1101	24-25	0.5255711861534991
1101	26-27	0.4983009988354965
1101	28-29	0.529603782431515
1101	30-31	0.5479188041253238
1101	32-33	0.5435255775024359
1101	34-35	0.47367297610173154
1101	36-37	0.48576764828654007
1101	38-39	0.503852754285397
1101	40-41	0.48907569623291636
1101	42-43	0.4756015996789529
1101	44-45	0.4263502098875662
1101	46-47	0.42207380211556256
1101	48-49	0.4781199702096117
1101	50-51	0.4406855241250014
1101	52-53	0.3779615128681897
1101	54-55	0.4180695024603196
1101	56-57	0.47715185890450584
1101	58-59	0.4543186952988165
1101	60-61	0.46381761656573417
1101	62-63	0.41494320356198955
1101	64-65	0.46489857931762657
1101	66-67	0.43364025210304646
1101	68-69	0.3756562526231946
1101	70-71	0.32815032463592786
1101	72-73	0.4249962407230683
1101	74-75	0.4122040998988723
1101	76-77	0.3717466088800805
1101	78-79	0.3620726658232485
1101	80-81	0.33553381021089734
1101	82-83	0.3395903742861819
1101	84-85	0.23399712057521782
1101	86-87	0.2920081129976779
1101	88-89	0.2911282447859378
1101	90-91	0.2534139111268914
1101	92-93	0.29909086226820136
1101	94-95	0.29728588024906344
1101	96-97	0.3113815765410166
1101	98-99	0.34017443452621166
1101	100	0.29804498649740907
1102	1	0.3052201578218039
1102	2	0.32934645493794434
1102	3	0.26548303272205587
1102	4	0.30192935019830713
1102	5	0.2747404662158246
1102	6	0.31900360544152306
1102	7	0.33538965860731906
1102	8	0.3172812805620211
1102	9	0.4074807182931579
1102	10-11	0.4403014226991928
1102	12-13	0.39558705883595024
1102	14-15	0.512797538507094
1102	16-17	0.486679912189679
1102	18-19	0.4538591120696651
1102	20-21	0.4789556096464693
1102	22-23	0.44416719730402576
1102	24-25	0.4994634577476944
1102	26-27	0.49054633776012935
1102	28-29	0.5234894885975052
1102	30-31	0.5181002345613805
1102	32-33	0.49280440473768294
1102	34-35	0.4830523803935094
1102	36-37	0.4596588092226028
1102	38-39	0.49405929894601996
1102	40-41	0.5007430792836658
1102	42-43	0.48424570225349584
1102	44-45	0.4244075364664752
1102	46-47	0.4692105243150593
1102	48-49	0.41794219378124353
1102	50-51	0.4304079599554882
1102	52-53	0.401089316747651
1102	54-55	0.3439254360368338
1102	56-57	0.39818496385967705
1102	58-59	0.41184266289835136
1102	60-61	0.42650526973728375
1102	62-63	0.3810872881242986
1102	64-65	0.46134716651560126
1102	66-67	0.36518606424170486
1102	68-69	0.3151460901704013
1102	70-71	0.3581761211383636
1102	72-73	0.32987279182044915
1102	74-75	0.3749828960932433
1102	76-77	0.36470995087777425
1102	78-79	0.3370720391358404
1102	80-81	0.24677172190568086
1102	82-83	0.3004616410448335
1102	84-85	0.29874536453264255
1102	86-87	0.24755459120894585
1102	88-89	0.2617155373239157
1102	90-91	0.3042242012581937
1102	92-93	0.2840388205114195
1102	94-95	0.2907021981211244
1102	96-97	0.29234398125834815
1102	98-99	0.3348288312096983
1102	100	0.36996342639403323
1103	1	0.24426693875699002
1103	2	0.2986346842992873
1103	3	0.2914298232919563
1103	4	0.2884783046573176
1103	5	0.23183330784885925
1103	6	0.30337585351499996
1103	7	0.32615042649270976
1103	8	0.34721548430589877
1103	9	0.45107573350442465
1103	10-11	0.38756106355106823
1103	12-13	0.39768741103358707
1103	14-15	0.47478400083070227
1103	16-17	0.48656011744671446
1103	18-19	0.4430629019139971
1103	20-21	0.4246816492970069
1103	22-23	0.4154585231581862
1103	24-25	0.4403056093505029
1103	26-27	0.42206563485984816
1103	28-29	0.40197486414559336
1103	30-31	0.4255394500178795
1103	32-33	0.4270695583782498
1103	34-35	0.38711622935942813
1103	36-37	0.3949063232776808
1103	38-39	0.41035307591377546
1103	40-41	0.39704007797924845
1103	42-43	0.4544544796828589
1103	44-45	0.39502371938706204
1103	46-47	0.34342303572806543
1103	48-49	0.35686360913709336
1103	50-51	0.38711650092352556
1103	52-53	0.3347991548392315
1103	54-55	0.27722009993178887
1103	56-57	0.33766513845534263
1103	58-59	0.3627675550218825
1103	60-61	0.36582601722383856
1103	62-63	0.33326716890731234
1103	64-65	0.29369634414535994
1103	66-67	0.32259647618927545
1103	68-69	0.35925139725704014
1103	70-71	0.351586577913757
1103	72-73	0.366577656254087
1103	74-75	0.3443693712885292
1103	76-77	0.27773761829514854
1103	78-79	0.311907439690124
1103	80-81	0.26343388674902
1103	82-83	0.26105454015431917
1103	84-85	0.2665646122183958
1103	86-87	0.25004974923273693
1103	88-89	0.2712783838929127
1103	90-91	0.31439447572143564
1103	92-93	0.2946202107299527
1103	94-95	0.3311732308328601
1103	96-97	0.3358765312907437
1103	98-99	0.2586311163515447
1103	100	0.35522545236903724
1104	1	0.1719922543098349
1104	2	0.1402294916379816
1104	3	0.1779317908426279
1104	4	0.17427330747833025
1104	5	0.14812917455613217
1104	6	0.12727589235180403
1104	7	0.15488566245106483
1104	8	0.1675773668286098
1104	9	0.16885074155752022
1104	10-11	0.25887057834253113
1104	12-13	0.24947103637897783
1104	14-15	0.37149483860869026
1104	16-17	0.3305229678328132
1104	18-19	0.3005959226514534
1104	20-21	0.31357764139838196
1104	22-23	0.35157448418398474
1104	24-25	0.39632502734233555
1104	26-27	0.3198323040924649
1104	28-29	0.33847212916173675
1104	30-31	0.35193053896029625
1104	32-33	0.3560551071829394
1104	34-35	0.38656222989981615
1104	36-37	0.3803780217380819
1104	38-39	0.36090150389225784
1104	40-41	0.32514938103398805
1104	42-43	0.3414907727198653
1104	44-45	0.32849030312554817
1104	46-47	0.29867900234988554
1104	48-49	0.27404917493225156
1104	50-51	0.32944102405841136
1104	52-53	0.2820916194672236
1104	54-55	0.27329338647899704
1104	56-57	0.291320960361773
1104	58-59	0.30071130432607873
1104	60-61	0.27202764309448924
1104	62-63	0.29227985747000673
1104	64-65	0.3705947241119887
1104	66-67	0.2881268213890493
1104	68-69	0.2628151006011308
1104	70-71	0.27035642248170433
1104	72-73	0.2830971693700235
1104	74-75	0.2036915735181637
1104	76-77	0.20594381400706396
1104	78-79	0.25295545982596934
1104	80-81	0.18957540400543138
1104	82-83	0.1760290559951656
1104	84-85	0.17084262033517206
1104	86-87	0.18870519646983297
1104	88-89	0.19070384355799774
1104	90-91	0.21638962311471488
1104	92-93	0.17716101365092385
1104	94-95	0.17923913460531082
1104	96-97	0.15745610957358025
1104	98-99	0.19312955884143435
1104	100	0.18694625594222813
1105	1	0.09687644635155124
1105	2	0.16870672173591927
1105	3	0.15153203732148768
1105	4	0.14800133773618995
1105	5	0.11067706220480034
1105	6	0.15022256545004353
1105	7	0.1400650371068508
1105	8	0.1972265388605976
1105	9	0.26759758500633524
1105	10-11	0.2599810256357884
1105	12-13	0.2591759329783443
1105	14-15	0.2710903497917556
1105	16-17	0.27776421401858187
1105	18-19	0.27219665525066006
1105	20-21	0.2680699395594246
1105	22-23	0.2807642391351379
1105	24-25	0.3219051511070745
1105	26-27	0.2616322126217909
1105	28-29	0.26045158536566504
1105	30-31	0.32353106310308277
1105	32-33	0.3649744358032194
1105	34-35	0.28136480708809586
1105	36-37	0.29354477477518515
1105	38-39	0.31430565959585266
1105	40-41	0.30713134705309386
1105	42-43	0.3407293856842628
1105	44-45	0.27772710251919364
1105	46-47	0.29618888631053863
1105	48-49	0.2887716173076029
1105	50-51	0.31502115616140713
1105	52-53	0.34192851066507046
1105	54-55	0.3185810625777634
1105	56-57	0.271067544073901
1105	58-59	0.2796428234771824
1105	60-61	0.3189372142871463
1105	62-63	0.26661845655121397
1105	64-65	0.32719243200869386
1105	66-67	0.29255964693646064
1105	68-69	0.3052964322873777
1105	70-71	0.29539881209256436
1105	72-73	0.3144661067801522
1105	74-75	0.26279459392646487
1105	76-77	0.2736634843290915
1105	78-79	0.25486075140853615
1105	80-81	0.2303841856902764
1105	82-83	0.2541712341061597
1105	84-85	0.25344722480372894
1105	86-87	0.19241457404818618
1105	88-89	0.1813627033931553
1105	90-91	0.1781376635202392
1105	92-93	0.23821018309804742
1105	94-95	0.24569451680388
1105	96-97	0.2286640894516978
1105	98-99	0.20519710038575312
1105	100	0.31033139625215256
1106	1	0.11023407004373453
1106	2	0.09534769522167252
1106	3	0.0824914726623227
1106	4	0.09580989938191209
1106	5	0.11009834353214387
1106	6	0.08909597636569799
1106	7	0.15085374289035514
1106	8	0.18720307324179686
1106	9	0.20612198208662136
1106	10-11	0.20120256278170956
1106	12-13	0.2232417507994171
1106	14-15	0.2560907039533902
1106	16-17	0.2638815325081936
1106	18-19	0.2843052270573807
1106	20-21	0.298772242999803
1106	22-23	0.27566454799974593
1106	24-25	0.29033927171244756
1106	26-27	0.2540225941373748
1106	28-29	0.2221248566830596
1106	30-31	0.22066810735918096
1106	32-33	0.26078574772233765
1106	34-35	0.245639165541661
1106	36-37	0.3011040248169081
1106	38-39	0.25355189521415866
1106	40-41	0.23727239979319847
1106	42-43	0.2702461898809787
1106	44-45	0.2016546012114162
1106	46-47	0.23379293607158047
1106	48-49	0.2260484177120361
1106	50-51	0.1726192093674399
1106	52-53	0.15059672320091266
1106	54-55	0.1407340295555244
1106	56-57	0.16384912777051852
1106	58-59	0.14752657107413114
1106	60-61	0.20782793128310573
1106	62-63	0.1673071763374665
1106	64-65	0.23083355381668724
1106	66-67	0.22383048803924765
1106	68-69	0.21494491403626625
1106	70-71	0.17720264918200002
1106	72-73	0.14227822932378587
1106	74-75	0.18449156551520218
1106	76-77	0.2000246482915955
1106	78-79	0.16896049198468432
1106	80-81	0.11504611812351584
1106	82-83	0.09386533412977371
1106	84-85	0.12687777668942601
1106	86-87	0.09933129993278556
1106	88-89	0.11608397381733937
1106	90-91	0.1412797961350165
1106	92-93	0.200217288595546
1106	94-95	0.091739606328197
1106	96-97	0.09227127613533526
1106	98-99	0.109545181213484
1106	100	0.22952171471349914
1107	1	0.09968844898411078
1107	2	0.12082245095139044
1107	3	0.13476666624143974
1107	4	0.09145441919353203
1107	5	0.10733444611945941
1107	6	0.11018490841980366
1107	7	0.11527424116480489
1107	8	0.12136932574679093
1107	9	0.1384917855125849
1107	10-11	0.15328817296214226
1107	12-13	0.20663714151427826
1107	14-15	0.18458408780524138
1107	16-17	0.15291749264960686
1107	18-19	0.19692829838312775
1107	20-21	0.2108238076833473
1107	22-23	0.27958821969578196
1107	24-25	0.32632663888674074
1107	26-27	0.2670194461612354
1107	28-29	0.22842252848129618
1107	30-31	0.2481455592996653
1107	32-33	0.2571954983231848
1107	34-35	0.20700211022789716
1107	36-37	0.19406582776821324
1107	38-39	0.220791515353298
1107	40-41	0.18692248918925003
1107	42-43	0.18754822867499144
1107	44-45	0.1807203306238634
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2216	64-65	-0.40876631518037954
2216	66-67	-0.4285412061691858
2216	68-69	-0.496996902689645
2216	70-71	-0.4767922088150556
2216	72-73	-0.421389714036259
2216	74-75	-0.382791159786521
2216	76-77	-0.4768189331583841
2216	78-79	-0.4208732653352456
2216	80-81	-0.40425417116507134
2216	82-83	-0.43616630578749493
2216	84-85	-0.36807136069766244
2216	86-87	-0.4106582649305679
2216	88-89	-0.4242610690509494
2216	90-91	-0.42327528015129445
2216	92-93	-0.3739596297451371
2216	94-95	-0.4405794296572765
2216	96-97	-0.4672119519372089
2216	98-99	-0.48742723489962714
2216	100	-0.5465532257135024
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	2242.0
1	581.0
2	448.0
3	380.0
4	297.0
5	463.0
6	698.0
7	1638.0
8	4687.0
9	7192.0
10	7985.0
11	9275.0
12	11229.0
13	13973.0
14	17248.0
15	18956.0
16	20484.0
17	22035.0
18	24634.0
19	26912.0
20	29925.0
21	33395.0
22	37570.0
23	43144.0
24	51299.0
25	61892.0
26	69270.0
27	70040.0
28	76227.0
29	93692.0
30	110730.0
31	129001.0
32	164198.0
33	217475.0
34	322255.0
35	495571.0
36	897608.0
37	1792962.0
38	2091942.0
39	639898.0
40	898.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.78942936104405	19.943379177056585	13.182731542240928	24.084459919658435
2	14.595304886159699	21.460021595574997	40.058688594723705	23.885984923541606
3	18.531131796575817	26.526514668469954	30.402664370272763	24.539689164681466
4	12.15780640968695	17.807187198172638	39.84375964501359	30.191246747126822
5	12.81726240001156	38.677909982985305	35.530709528034194	12.974118088968947
6	29.187160494864955	38.23936546156148	18.965791030135925	13.60768301343763
7	25.892176861500747	33.05311511117643	23.512508817975576	17.542199209347245
8	25.056205468568475	35.017599466637456	21.60122244677703	18.324972618017036
9	26.130324955497464	16.21946134698056	21.06676069488958	36.583453002632396
10-11	24.22793277981498	26.618702343161154	28.913329185820025	20.240035691203843
12-13	25.1842532519229	24.421521249949688	28.308293591019666	22.08593190710775
14-15	22.815274790053426	25.637808330253037	26.18700822936337	25.359908650330166
16-17	22.084513586748102	28.397580095253804	27.05846746020322	22.459438857794872
18-19	21.96334130661162	28.742079091085674	27.85894428857305	21.435635313729655
20-21	23.13324043545127	27.620409063229783	26.99185127724082	22.254499224078124
22-23	22.826913822100856	27.546139234924897	26.52651669337327	23.100430249600976
24-25	22.823381965649844	27.89467698280627	26.672162432313627	22.60977861923025
26-27	22.86375999147982	27.468104153679455	26.92381990326024	22.744315951580482
28-29	22.272592446471396	28.218862538844487	27.020459796276064	22.488085218408056
30-31	22.981629587620397	27.66022404050305	26.69271044537081	22.665435926505744
32-33	22.7027996114916	27.953941134016354	26.757741108667428	22.58551814582462
34-35	22.865169686744338	27.959347867331942	27.049571400341772	22.125911045581947
36-37	23.014091851182485	27.533899774351756	27.163517485768924	22.288490888696835
38-39	22.90127189592824	27.578578675082575	27.23934644650382	22.28080298248536
40-41	22.82431694001631	27.517576698604756	27.122034078841512	22.536072282537422
42-43	22.90850985175008	27.301275049030373	26.772338773767572	23.01787632545198
44-45	22.95350229442689	27.531546563770988	27.01108112701555	22.50387001478657
46-47	22.93275194625672	27.461135609192304	27.158317436030455	22.447795008520515
48-49	22.808669208063424	27.434350625404925	27.359297437007175	22.39768272952448
50-51	22.305849044064125	27.64833811557392	27.073326550721305	22.97248628964065
52-53	22.196953044141647	27.894260968159063	26.60604077989113	23.302745207808158
54-55	22.39868432821501	27.467872939928572	26.696466249955837	23.436976481900583
56-57	22.962043906548338	27.03623870042407	26.873654448832756	23.12806294419484
58-59	22.867555056637013	27.181516188847876	27.262201493108318	22.68872726140679
60-61	22.68590282730001	27.633470961535256	27.20175677914017	22.47886943202457
62-63	22.302311033027657	27.409938673475253	27.20884678220464	23.078903511292452
64-65	22.015162306349836	27.772899314919318	26.914584675914043	23.297353702816796
66-67	22.758739774508324	27.059277031326857	26.785633944852478	23.396349249312344
68-69	22.58494135829855	27.071742648926307	26.94152262522832	23.401793367546823
70-71	22.71760887504305	26.879289368133698	26.99991645559444	23.403185301228813
72-73	22.85212734019966	26.684231484384068	27.481688920536733	22.981952254879534
74-75	22.629940636079258	27.479729220706588	26.99638711794447	22.89394302526968
76-77	22.60867046601824	27.022901194528536	27.11787184820248	23.250556491250745
78-79	22.497448544274473	27.09460097649587	27.196554112733818	23.211396366495844
80-81	22.504961299727526	27.037666469955195	27.239579792773167	23.21779243754411
82-83	22.632730261170128	26.687224034188628	27.61119553525873	23.068850169382515
84-85	22.810348684602015	26.971428699035904	26.931258660761397	23.28696395560068
86-87	22.319284125299745	27.160660860160124	27.206195434287068	23.31385958025306
88-89	22.72330819285129	26.720573906002848	26.953374471985512	23.60274342916035
90-91	22.859899434648284	26.3650340997457	27.30226482209285	23.472801643513165
92-93	22.89823657595225	26.744423006361746	27.213260937280502	23.144079480405498
94-95	22.277209938900633	26.676147735231908	27.5732547177401	23.47338760812736
96-97	22.4099634979788	26.477139248164228	27.557922709127446	23.554974544729525
98-99	22.608033697219604	26.80283967252534	26.924291272345247	23.664835357909805
100	22.840642121493516	26.266003081663285	26.853723865026147	24.03963093181705
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3352.0
1	2162.5
2	1057.5
3	1116.5
4	1048.5
5	883.0
6	752.0
7	767.5
8	837.5
9	900.5
10	842.5
11	763.0
12	820.0
13	914.0
14	965.5
15	1142.5
16	1366.5
17	1552.0
18	1887.5
19	2381.0
20	3490.0
21	5193.0
22	7391.5
23	10759.5
24	15246.5
25	20767.5
26	29564.0
27	40784.0
28	51894.5
29	67015.0
30	84408.5
31	100400.0
32	119004.5
33	137135.5
34	154169.5
35	171938.5
36	190807.0
37	213804.0
38	225176.5
39	221198.5
40	219231.0
41	220453.5
42	224164.0
43	237228.0
44	267753.0
45	298155.0
46	310136.5
47	325826.0
48	360019.5
49	366805.5
50	357646.5
51	342089.0
52	302793.0
53	274852.0
54	256102.5
55	232027.0
56	207148.0
57	179336.0
58	152892.5
59	135598.0
60	113160.0
61	87898.5
62	67473.5
63	51437.0
64	37579.5
65	25981.5
66	18258.0
67	12715.0
68	9420.5
69	7371.5
70	5773.0
71	4673.0
72	3697.5
73	2866.0
74	2188.0
75	1670.5
76	1185.5
77	756.0
78	470.0
79	301.0
80	207.5
81	158.0
82	113.0
83	100.0
84	95.0
85	69.5
86	58.0
87	50.0
88	46.5
89	40.5
90	34.0
91	35.0
92	33.5
93	28.0
94	24.5
95	24.0
96	22.0
97	17.5
98	16.0
99	17.5
100	26.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09975921050335097
2	0.06430151689902916
3	0.07994384509160932
4	0.0828177292142394
5	0.08325078024641654
6	0.09025833331255563
7	0.08713511677745993
8	0.08878858435486355
9	0.07367116650431627
10-11	0.08067871957045537
12-13	0.07026581066037789
14-15	0.07992416095378309
16-17	0.08090180646581935
18-19	0.08091492922437017
20-21	0.07264759133735213
22-23	0.07631540235230697
24-25	0.07876279682203531
26-27	0.07199145340981103
28-29	0.08100022715495052
30-31	0.08533073747672186
32-33	0.08679392505513855
34-35	0.08891325056109635
36-37	0.08830304228848311
38-39	0.08966780917776863
40-41	0.08535698299382351
42-43	0.09127534710024436
44-45	0.08769939539514528
46-47	0.0836444630029412
48-49	0.09292881467764796
50-51	0.08874921607921107
52-53	0.09460852777215321
54-55	0.08655771540122376
56-57	0.09271885054083481
58-59	0.09761363948029153
60-61	0.09613732914332401
62-63	0.09725932499941932
64-65	0.098722512577836
66-67	0.10098618842785284
68-69	0.09795483120261289
70-71	0.1001397705013248
72-73	0.09715434293101274
74-75	0.10141923946002998
76-77	0.09804012913319324
78-79	0.10534950564600125
80-81	0.10073685601538723
82-83	0.10489020909672246
84-85	0.10117646842683976
86-87	0.09779735810000302
88-89	0.09704279948333075
90-91	0.09696406293202581
92-93	0.09120973330749024
94-95	0.09096040089502462
96-97	0.08920851262848985
98-99	0.10303333876178111
100	0.24421453663080261
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7620349.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.52882173624784
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.08146117490669	37.33825402808696
2	14.20568551169994	14.924158437705696
3	5.699015831977517	8.980877601300035
4	2.9006781304906313	6.094768177231001
5	1.721436433694789	4.52125137779179
6	1.0865218386733195	3.4244227185726626
7	0.7174623760130405	2.638121727643768
8	0.5139177600337451	2.1596395523123513
9	0.38220628196893247	1.806916108681812
>10	1.6277789240916516	14.013488436280378
>50	0.04779996020056239	1.6998630866417095
>100	0.015465133042771109	1.380007957840764
>500	3.11259937546836E-4	0.10823777865531356
>1k	1.0375330754468292E-4	0.09856395525280114
>5k	1.0375330754468292E-4	0.37716994875698656
>10k+	5.187665377234146E-5	0.4342591072459604
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	15936	0.20912428026590382	No Hit
CATGTAAGCAGTGGTATCAACGCAGAGTACATGTAAGCAGTGGTATCAAC	7832	0.10277744497004009	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.3122758550822279E-5	0.0
5	0.0	0.0	0.0	1.3122758550822279E-5	0.0
6	0.0	0.0	0.0	1.3122758550822279E-5	0.0
7	0.0	0.0	0.0	1.3122758550822279E-5	0.0
8	0.0	0.0	0.0	1.3122758550822279E-5	1.3122758550822279E-5
9	0.0	0.0	0.0	2.6245517101644558E-5	1.3122758550822279E-5
10-11	0.0	0.0	0.0	2.6245517101644558E-5	1.3122758550822279E-5
12-13	0.0	0.0	0.0	4.5929654927877976E-5	2.6245517101644558E-5
14-15	0.0	0.0	0.0	5.9052413478700256E-5	3.9368275652466835E-5
16-17	0.0	0.0	0.0	8.529793058034482E-5	6.561379275411139E-5
18-19	0.0	0.0	0.0	1.7059586116068964E-4	6.561379275411139E-5
20-21	0.0	0.0	0.0	2.165255160885676E-4	9.842068913116708E-5
22-23	0.0	0.0	0.0	3.7399861869843494E-4	1.0498206840657823E-4
24-25	0.0	0.0	0.0	8.529793058034481E-4	1.0498206840657823E-4
26-27	0.0	0.0	0.0	0.0019552910240725196	1.1810482695740051E-4
28-29	0.0	0.0	0.0	0.005222857903227267	1.1810482695740051E-4
30-31	0.0	0.0	0.0	0.016738078531573816	1.1810482695740051E-4
32-33	0.0	0.0	0.0	0.042990157012493785	1.1810482695740051E-4
34-35	0.0	0.0	0.0	0.08288334300699352	1.1810482695740051E-4
36-37	0.0	0.0	0.0	0.14600381163644865	1.1810482695740051E-4
38-39	0.0	0.0	0.0	0.24067139182208058	1.1810482695740051E-4
40-41	0.0	0.0	0.0	0.3482714505595479	1.1810482695740051E-4
42-43	0.0	0.0	0.0	0.45494635481918216	1.1810482695740051E-4
44-45	0.0	0.0	0.0	0.5874796548032117	1.377889647836339E-4
46-47	0.0	0.0	0.0	0.7492832677348504	1.5747310260986734E-4
48-49	0.0	0.0	0.0	0.924682058525141	1.5747310260986734E-4
50-51	0.0	0.0	0.0	1.1104412671913058	1.5747310260986734E-4
52-53	0.0	0.0	0.0	1.2991530965314055	1.7059586116068964E-4
54-55	0.0	0.0	0.0	1.5050557395730826	1.9684137826233417E-4
56-57	0.0	0.0	0.0	1.751855459638397	1.9684137826233417E-4
58-59	0.0	0.0	0.0	2.0306222195335146	1.9684137826233417E-4
60-61	0.0	0.0	0.0	2.3224658083245266	1.9684137826233417E-4
62-63	0.0	0.0	0.0	2.6217696853516816	1.9684137826233417E-4
64-65	0.0	0.0	0.0	2.940987348479709	1.9684137826233417E-4
66-67	0.0	0.0	0.0	3.3024340486242822	1.9684137826233417E-4
68-69	0.0	0.0	0.0	3.7140359319500984	1.9684137826233417E-4
70-71	0.0	0.0	0.0	4.160806808192119	1.9684137826233417E-4
72-73	0.0	0.0	0.0	4.605648638927168	2.034027575377453E-4
74-75	1.3122758550822279E-5	0.0	0.0	5.0450182793465235	2.0996413681315646E-4
76-77	1.3122758550822279E-5	0.0	0.0	5.523316583006894	2.0996413681315646E-4
78-79	1.3122758550822279E-5	0.0	0.0	6.061526840831043	2.0996413681315646E-4
80-81	1.3122758550822279E-5	0.0	0.0	6.642327011531886	2.165255160885676E-4
82-83	1.3122758550822279E-5	0.0	0.0	7.242922863506646	2.493324124656233E-4
84-85	1.3122758550822279E-5	0.0	0.0	7.836478355518888	2.493324124656233E-4
86-87	1.3122758550822279E-5	0.0	0.0	8.44654883916734	2.6245517101644556E-4
88	1.3122758550822279E-5	0.0	0.0	8.932543640717768	2.755779295672678E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	2945	0.0	23.634678	1
CATGGGG	16415	0.0	21.62275	4
TATTCGC	665	0.0	20.491596	9
TAGTACT	4675	0.0	20.413586	4
CTAGTAC	4035	0.0	19.923384	3
TACCTGG	6010	0.0	18.77359	2
ATGGGGG	11350	0.0	18.596556	5
CTGTGCG	4565	0.0	18.528112	9
ACATGGG	41205	0.0	18.460318	3
TACATGG	43750	0.0	18.299835	2
TAGGCAT	4255	0.0	17.455791	5
TACTGTG	6815	0.0	17.377312	7
CTCGTCG	2145	0.0	17.3061	9
GCTATTG	4950	0.0	17.088495	8
CGACGAG	1655	0.0	16.895561	24-25
GCATCGT	3470	0.0	16.793018	8
CTAGGCA	4685	0.0	16.757597	4
CTATTGA	5325	0.0	16.677912	9
TCGCCGT	1870	0.0	16.611969	94
TGGGGGG	10190	0.0	16.326002	6
>>END_MODULE