FastQCFastQC Report
Mon 16 Jan 2017
ERR1146635_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146635_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7620349
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT206370.2708143682133194No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191010.25065781107925633No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175540.23035690360113428No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92280.12109681590698798No Hit
CATGTAAGCAGTGGTATCAACGCAGAGTACATGTAAGCAGTGGTATCAAC81530.10698985046485403No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG30050.024.0966661
GAGTACT219350.021.79050812-13
CATGGGG156650.021.4003014
GCTATTG50850.019.8714128
GTATAAG31100.019.8057651
TAGGCAT42550.019.7724425
TATTCGC5750.019.616649
AGTACTT238200.019.59257712-13
CGACGAG14700.019.50252524-25
CTATTGA53800.019.2185949
GTGATCG15950.019.152878
GTACTTT253050.018.88855214-15
GGGCTAT57900.018.832496
CTAGGCA45250.018.4953354
ATTTAGG59300.018.1577281
CTAGTAC39900.018.1471583
GGCTATT57650.017.7720877
ACATGGG409750.017.6595823
TGATCGC17700.017.5247529
TAGTACT49550.017.4596274
Produced by FastQC (version 0.11.5)