##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146635_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7620349
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.34929174503687	33.0	31.0	34.0	30.0	34.0
2	32.436602050640985	34.0	31.0	34.0	30.0	34.0
3	32.54313299823932	34.0	31.0	34.0	30.0	34.0
4	36.018548231846076	37.0	35.0	37.0	35.0	37.0
5	36.06041232494732	37.0	35.0	37.0	35.0	37.0
6	36.127499672259106	37.0	36.0	37.0	35.0	37.0
7	36.094903527384375	37.0	36.0	37.0	35.0	37.0
8	36.104612793980955	37.0	36.0	37.0	35.0	37.0
9	37.8988865208142	39.0	38.0	39.0	35.0	39.0
10-11	37.84302208468405	39.0	38.0	39.0	35.0	39.0
12-13	37.81577444812567	39.0	38.0	39.0	35.0	39.0
14-15	39.1667871117189	40.0	39.0	41.0	36.0	41.0
16-17	39.115868774514134	40.0	39.0	41.0	36.0	41.0
18-19	39.21103469145574	40.0	39.0	41.0	36.0	41.0
20-21	39.264883799941444	40.5	39.0	41.0	36.0	41.0
22-23	39.23717325807519	40.5	39.0	41.0	36.0	41.0
24-25	39.227083562708216	41.0	39.0	41.0	36.0	41.0
26-27	39.146326828338175	40.5	39.0	41.0	35.5	41.0
28-29	39.090307281201945	40.0	39.0	41.0	35.0	41.0
30-31	38.974926870147286	40.0	38.5	41.0	35.0	41.0
32-33	38.8812722356942	40.0	38.0	41.0	35.0	41.0
34-35	38.819205655803955	40.0	38.0	41.0	35.0	41.0
36-37	38.76473964643877	40.0	38.0	41.0	35.0	41.0
38-39	38.690446461179135	40.0	38.0	41.0	35.0	41.0
40-41	38.61394504372437	40.0	38.0	41.0	35.0	41.0
42-43	38.52745950349518	40.0	38.0	41.0	35.0	41.0
44-45	38.39844428385104	40.0	38.0	41.0	34.0	41.0
46-47	38.19301419134478	40.0	38.0	41.0	33.5	41.0
48-49	37.93775672216587	40.0	38.0	41.0	33.0	41.0
50-51	37.82889694422132	40.0	37.0	41.0	33.0	41.0
52-53	37.74117071278494	40.0	37.0	41.0	33.0	41.0
54-55	37.58906409667064	40.0	37.0	41.0	33.0	41.0
56-57	37.42306271011997	40.0	36.0	41.0	33.0	41.0
58-59	37.247811287908206	39.0	36.0	41.0	32.0	41.0
60-61	37.031591925776624	39.0	35.0	41.0	32.0	41.0
62-63	36.7905098572257	39.0	35.0	41.0	32.0	41.0
64-65	36.53208133905678	38.5	35.0	40.5	31.0	41.0
66-67	36.25611937196052	37.5	35.0	40.0	31.0	41.0
68-69	35.95656603129332	37.0	35.0	40.0	31.0	41.0
70-71	35.634608139338496	36.5	35.0	39.0	31.0	41.0
72-73	35.30094986463219	36.0	35.0	39.0	31.0	41.0
74-75	34.97680276848213	35.5	35.0	39.0	30.0	40.5
76-77	34.6698949090127	35.0	34.5	37.0	30.0	39.5
78-79	34.379076798188635	35.0	34.0	37.0	30.0	39.0
80-81	34.112929473440126	35.0	34.0	36.5	30.0	39.0
82-83	33.85937415727285	35.0	34.0	36.0	30.0	37.5
84-85	33.64677569229441	35.0	34.0	36.0	30.0	37.0
86-87	33.46142552001227	35.0	34.0	35.0	29.0	37.0
88-89	33.26741616427279	35.0	34.0	35.0	29.0	36.0
90-91	33.17758832305449	35.0	34.0	35.0	29.0	36.0
92-93	33.07433006021115	35.0	34.0	35.0	29.0	36.0
94-95	33.00140052640634	35.0	34.0	35.0	29.0	36.0
96-97	32.9465112424641	35.0	34.0	35.0	29.0	35.5
98-99	32.8840194851968	35.0	34.0	35.0	29.0	35.0
100	32.11948009205353	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.34217472987852204
1101	2	0.3487279068982474
1101	3	0.3521539843796688
1101	4	0.2407804349583742
1101	5	0.213605052734259
1101	6	0.23439606294166282
1101	7	0.24198075290674836
1101	8	0.23807536264216367
1101	9	0.2910565350902701
1101	10-11	0.296421017179469
1101	12-13	0.33236754599092677
1101	14-15	0.4673994209300574
1101	16-17	0.5025054779641778
1101	18-19	0.460806197858453
1101	20-21	0.3811085623253021
1101	22-23	0.3602244206375289
1101	24-25	0.3908943888943739
1101	26-27	0.3917291480159548
1101	28-29	0.4278999185542318
1101	30-31	0.45783826108566217
1101	32-33	0.43446658892269596
1101	34-35	0.4307804393341783
1101	36-37	0.4686739056950344
1101	38-39	0.485708343429728
1101	40-41	0.45322800115704354
1101	42-43	0.48348699386144034
1101	44-45	0.4594399871817032
1101	46-47	0.4661732023007943
1101	48-49	0.5739756988332232
1101	50-51	0.5063601550343435
1101	52-53	0.47855906487031064
1101	54-55	0.5165033536811237
1101	56-57	0.4678255487247682
1101	58-59	0.49767701027900557
1101	60-61	0.4802574655146188
1101	62-63	0.47588137787587215
1101	64-65	0.4354081381664656
1101	66-67	0.41742115817689296
1101	68-69	0.4126893261896214
1101	70-71	0.3873691701165711
1101	72-73	0.3308495576273387
1101	74-75	0.40275996786806445
1101	76-77	0.3716686642578324
1101	78-79	0.2727933056027041
1101	80-81	0.3568890326047267
1101	82-83	0.38029293612994763
1101	84-85	0.3174605966266242
1101	86-87	0.3425983047985284
1101	88-89	0.39418111135416467
1101	90-91	0.3861668547969259
1101	92-93	0.39354813626800933
1101	94-95	0.38683589294208787
1101	96-97	0.43488238307041627
1101	98-99	0.4510313326436872
1101	100	0.45327829972053024
1102	1	0.29921279317986205
1102	2	0.27063951365777683
1102	3	0.2820625598366675
1102	4	0.19184872809505293
1102	5	0.18317217988708023
1102	6	0.22688552797527706
1102	7	0.18075440586777347
1102	8	0.22894970057195252
1102	9	0.2788888322045082
1102	10-11	0.2832999207543381
1102	12-13	0.2829240559196009
1102	14-15	0.4411920958393125
1102	16-17	0.47558143885436976
1102	18-19	0.4269071048667641
1102	20-21	0.3629090305327338
1102	22-23	0.38388666975452423
1102	24-25	0.4067918256358354
1102	26-27	0.3832976760482012
1102	28-29	0.40582168660411355
1102	30-31	0.41352280043221157
1102	32-33	0.44064602026620037
1102	34-35	0.4054138191432841
1102	36-37	0.4238484630563377
1102	38-39	0.4051514808527159
1102	40-41	0.42644982656292285
1102	42-43	0.4689349496761608
1102	44-45	0.396887727264037
1102	46-47	0.41445941192523605
1102	48-49	0.5044950384296882
1102	50-51	0.4764135835600172
1102	52-53	0.3983166566559646
1102	54-55	0.4634051883755532
1102	56-57	0.45619313126352523
1102	58-59	0.37408006785982195
1102	60-61	0.3791069353370702
1102	62-63	0.40151408067842453
1102	64-65	0.42272109038990635
1102	66-67	0.39389248152501466
1102	68-69	0.36889370533161525
1102	70-71	0.3860840638532892
1102	72-73	0.3927032596212072
1102	74-75	0.38774240830554163
1102	76-77	0.3859599339716411
1102	78-79	0.35389980008459077
1102	80-81	0.3372132117288231
1102	82-83	0.3250138583578419
1102	84-85	0.30182306539612114
1102	86-87	0.33443956557988486
1102	88-89	0.35917679843271344
1102	90-91	0.28832471046113284
1102	92-93	0.2906010734109046
1102	94-95	0.3115431412995804
1102	96-97	0.30517565275149394
1102	98-99	0.30972792158354423
1102	100	0.3270543746068313
1103	1	0.27403950018286594
1103	2	0.22350109833985243
1103	3	0.21358113882076424
1103	4	0.20087033853354086
1103	5	0.16204743781707265
1103	6	0.18456671303579952
1103	7	0.18862672665046176
1103	8	0.20815678232359858
1103	9	0.19772538043812915
1103	10-11	0.21722342000556694
1103	12-13	0.2024120383906194
1103	14-15	0.3466366744226761
1103	16-17	0.44987185493219073
1103	18-19	0.33414465495872747
1103	20-21	0.32166432615944984
1103	22-23	0.323539926536732
1103	24-25	0.3296041013437119
1103	26-27	0.338418865948384
1103	28-29	0.36174002837068286
1103	30-31	0.4046313810924502
1103	32-33	0.3618106014326017
1103	34-35	0.3437556172645415
1103	36-37	0.34438358323544094
1103	38-39	0.32382030012536944
1103	40-41	0.3380565226864718
1103	42-43	0.34947438616539017
1103	44-45	0.36264011849386435
1103	46-47	0.4297090324528057
1103	48-49	0.47452013602676857
1103	50-51	0.44814556039469267
1103	52-53	0.4355693127453293
1103	54-55	0.4527447999979515
1103	56-57	0.42763971153979696
1103	58-59	0.391281597380825
1103	60-61	0.3805436562122182
1103	62-63	0.40434640486889606
1103	64-65	0.4020607787201129
1103	66-67	0.36123849645387907
1103	68-69	0.3592082405195782
1103	70-71	0.30501745433121386
1103	72-73	0.3132455065538693
1103	74-75	0.3130671312149076
1103	76-77	0.3124159610045538
1103	78-79	0.30783364020082615
1103	80-81	0.2837606886105277
1103	82-83	0.28735103312014587
1103	84-85	0.3090690751077716
1103	86-87	0.3034993634488288
1103	88-89	0.3986024071213947
1103	90-91	0.3320542472259618
1103	92-93	0.3235538979473489
1103	94-95	0.3298366635539054
1103	96-97	0.29422479204367136
1103	98-99	0.32265964440114914
1103	100	0.32486509031731714
1104	1	0.20024979200682935
1104	2	0.18891002103110566
1104	3	0.2167787907430423
1104	4	0.16740635652339364
1104	5	0.11565369398723391
1104	6	0.15171406051496916
1104	7	0.16731183461298116
1104	8	0.136309384476192
1104	9	0.2026544851297416
1104	10-11	0.1893451034025233
1104	12-13	0.20835743235515736
1104	14-15	0.3254940127124897
1104	16-17	0.3624559600178969
1104	18-19	0.30449550697109373
1104	20-21	0.2839007145965695
1104	22-23	0.30611288559330063
1104	24-25	0.27632417040144475
1104	26-27	0.273082725201931
1104	28-29	0.263610282580494
1104	30-31	0.2962297460007548
1104	32-33	0.28114341605593296
1104	34-35	0.27481995212834676
1104	36-37	0.20697805434291894
1104	38-39	0.25251737399370455
1104	40-41	0.33052085932906294
1104	42-43	0.32403193133604447
1104	44-45	0.28201287399202357
1104	46-47	0.3276452996156536
1104	48-49	0.3727301827055598
1104	50-51	0.3044975191383372
1104	52-53	0.2722265712760574
1104	54-55	0.2641247802248117
1104	56-57	0.28070250294997834
1104	58-59	0.24917234809854705
1104	60-61	0.2151518619918562
1104	62-63	0.21991417099835786
1104	64-65	0.26207449887575507
1104	66-67	0.22916911239695992
1104	68-69	0.2116828266428712
1104	70-71	0.20872029647153312
1104	72-73	0.24885382897680586
1104	74-75	0.23526365135980143
1104	76-77	0.22769073367535242
1104	78-79	0.2411279936120394
1104	80-81	0.22248043789592487
1104	82-83	0.19207561333971057
1104	84-85	0.18166270384818972
1104	86-87	0.2076733637692172
1104	88-89	0.260313312881685
1104	90-91	0.21741809919058142
1104	92-93	0.24060565107778586
1104	94-95	0.2513715830930252
1104	96-97	0.20741837116212736
1104	98-99	0.22041922916325518
1104	100	0.31126743569619464
1105	1	0.1808879437822739
1105	2	0.14012788591215752
1105	3	0.17540874017569053
1105	4	0.1329799889555403
1105	5	0.10619477595103888
1105	6	0.11782321746304802
1105	7	0.11693042192057135
1105	8	0.13658809069565336
1105	9	0.1567805253793395
1105	10-11	0.15657554737715174
1105	12-13	0.15552102852839766
1105	14-15	0.26718594082170455
1105	16-17	0.33094277077241685
1105	18-19	0.25842424322912194
1105	20-21	0.22692843137768648
1105	22-23	0.23226626448408894
1105	24-25	0.21754498702526348
1105	26-27	0.2194541870191813
1105	28-29	0.20309549113905234
1105	30-31	0.23824259275234994
1105	32-33	0.25590893386373637
1105	34-35	0.21940452560360058
1105	36-37	0.22617918267576442
1105	38-39	0.23961558989867626
1105	40-41	0.22953769551943282
1105	42-43	0.24040599468929003
1105	44-45	0.23368194277137633
1105	46-47	0.23830890808782357
1105	48-49	0.3510820888101236
1105	50-51	0.2874503708845566
1105	52-53	0.23905031679581157
1105	54-55	0.23832934183473498
1105	56-57	0.22747659832212008
1105	58-59	0.19359026067442642
1105	60-61	0.18102139829724706
1105	62-63	0.19444246541218746
1105	64-65	0.20200695066083085
1105	66-67	0.18250364365979976
1105	68-69	0.14774799511943115
1105	70-71	0.13892742292910754
1105	72-73	0.11147250842213197
1105	74-75	0.1362784380067552
1105	76-77	0.08068112425178953
1105	78-79	0.16912558635742414
1105	80-81	0.14875513841702315
1105	82-83	0.09985345550735758
1105	84-85	0.10703082744448977
1105	86-87	0.08345596124447496
1105	88-89	0.17923627698173306
1105	90-91	0.12331712373965331
1105	92-93	0.13533662348940823
1105	94-95	0.1438052616440757
1105	96-97	0.17604370120073298
1105	98-99	0.1636605095358803
1105	100	0.1181004700335464
1106	1	0.10478231826569129
1106	2	0.09956046184890965
1106	3	0.09244824064005996
1106	4	0.08554641299874532
1106	5	0.09668558140919714
1106	6	0.07821814170647201
1106	7	0.08899529050841437
1106	8	0.10757717738754735
1106	9	0.12803746159048046
1106	10-11	0.1017614302140828
1106	12-13	0.09946978310362198
1106	14-15	0.20554680872181308
1106	16-17	0.27173159224508225
1106	18-19	0.18140591933882888
1106	20-21	0.19141354965839952
1106	22-23	0.17304816461603423
1106	24-25	0.16166170916997658
1106	26-27	0.18382098766335986
1106	28-29	0.18854963422813853
1106	30-31	0.23277446982832117
1106	32-33	0.1893975326056605
1106	34-35	0.18008468002608424
1106	36-37	0.18049306789662012
1106	38-39	0.1807590004785382
1106	40-41	0.162034368819306
1106	42-43	0.19627448943392523
1106	44-45	0.19151855394617456
1106	46-47	0.20263704563161866
1106	48-49	0.28178864510059753
1106	50-51	0.25880117721074214
1106	52-53	0.18788284578021575
1106	54-55	0.21902400360563945
1106	56-57	0.15806343633681053
1106	58-59	0.20002378784532482
1106	60-61	0.1344468416696074
1106	62-63	0.17423132286490528
1106	64-65	0.19264988918888548
1106	66-67	0.1727894555588918
1106	68-69	0.19388643452135312
1106	70-71	0.1543993297619366
1106	72-73	0.16147874035728904
1106	74-75	0.1777976601069895
1106	76-77	0.11752907802152635
1106	78-79	0.10287544070003918
1106	80-81	0.11646047185847408
1106	82-83	0.08171109351516748
1106	84-85	0.12350640144192226
1106	86-87	0.12036463363527616
1106	88-89	0.21692817047101443
1106	90-91	0.13491251587662845
1106	92-93	0.15373534793997123
1106	94-95	0.1533477259156797
1106	96-97	0.10732402845118116
1106	98-99	0.1002081589739916
1106	100	0.15759253057361633
1107	1	0.11599245648505274
1107	2	0.1141319359070323
1107	3	0.11514089633853786
1107	4	0.07849859531033587
1107	5	0.07749382074806732
1107	6	0.09344610848752666
1107	7	0.07762504566895245
1107	8	0.0596218924674119
1107	9	0.0912455577817255
1107	10-11	0.14125871557489234
1107	12-13	0.12241011947064351
1107	14-15	0.20524263567077128
1107	16-17	0.27574132689432673
1107	18-19	0.18240134898784532
1107	20-21	0.18437755151381197
1107	22-23	0.21191411612127098
1107	24-25	0.175176172105715
1107	26-27	0.1831217239974876
1107	28-29	0.17224795611692656
1107	30-31	0.21700711152120533
1107	32-33	0.15974010866594313
1107	34-35	0.16050316969020884
1107	36-37	0.15633839281283457
1107	38-39	0.17952180427511877
1107	40-41	0.15883656127608248
1107	42-43	0.194092224738462
1107	44-45	0.20053756064108796
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2216	56-57	-0.5502312866466568
2216	58-59	-0.5814846445219786
2216	60-61	-0.5939951196173894
2216	62-63	-0.5944159484590301
2216	64-65	-0.49771603600185443
2216	66-67	-0.5269030472943186
2216	68-69	-0.5088404054797735
2216	70-71	-0.5070157096528405
2216	72-73	-0.4978192693470831
2216	74-75	-0.4721592699236581
2216	76-77	-0.4607836245870658
2216	78-79	-0.5002473859706029
2216	80-81	-0.5297738650369865
2216	82-83	-0.5045002449978142
2216	84-85	-0.4501722300869062
2216	86-87	-0.42476673852121394
2216	88-89	-0.3642785167836351
2216	90-91	-0.43121493703601743
2216	92-93	-0.4315081713822977
2216	94-95	-0.3787396192821717
2216	96-97	-0.42898947823726274
2216	98-99	-0.4478561079727186
2216	100	-0.6097109967228995
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	47.0
8	260.0
9	571.0
10	598.0
11	347.0
12	372.0
13	510.0
14	873.0
15	1590.0
16	2841.0
17	5121.0
18	8520.0
19	12778.0
20	18181.0
21	26760.0
22	38484.0
23	45578.0
24	45860.0
25	42071.0
26	41308.0
27	48274.0
28	59574.0
29	74199.0
30	92925.0
31	118350.0
32	152060.0
33	198624.0
34	287729.0
35	440965.0
36	798992.0
37	1812595.0
38	2414410.0
39	827072.0
40	1908.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.41060912524506	19.93255519538508	13.436612920179991	24.22022275918987
2	14.800792381692283	21.61074741488426	39.63348081676469	23.954979386658774
3	18.393008921779224	26.565819036182237	30.786181353301643	24.254990688736893
4	12.310670240118247	18.154098600427307	39.300435539028946	30.234795620425498
5	12.526866156215167	38.53465704624902	35.5490459414224	13.38943085611341
6	29.150787351561213	38.44145965814065	18.561213316157428	13.846539674140706
7	25.45930329147784	33.4214483174386	23.379017173235965	17.740231217847587
8	25.136703522934656	34.700445112365976	21.42094239312428	18.74190897157509
9	26.249474597140836	15.373953639619634	21.156239292640763	37.22033247059876
10-11	24.27701885499736	26.252238991157938	28.885539957171204	20.585202196673492
12-13	25.590068118927363	24.14145992526064	28.54092378183729	21.72754817397471
14-15	22.94460475356306	25.11777350821426	26.937559405103208	25.000062333119477
16-17	21.638518472248485	27.96500883230278	27.833800483260358	22.56267221218838
18-19	21.465231306295472	27.690419829720188	29.57277690843039	21.27157195555395
20-21	22.530116300028077	26.591264017513144	29.01224729862545	21.866372383833333
22-23	22.19054534116482	26.529441105650147	28.59091493053665	22.689098622648384
24-25	22.22726281959002	26.888276376843105	28.73050827462102	22.15395252894585
26-27	22.32775568884372	26.474852176706488	28.93500105605642	22.262391078393374
28-29	21.67681701171541	27.177930248203214	29.10473845126637	22.040514288815004
30-31	22.443925175696098	26.627043718504893	28.718120727425045	22.21091037837396
32-33	22.260163866962156	26.861691517740443	28.768098958470027	22.11004565682737
34-35	22.384793107493714	26.918360796259062	28.996891737291303	21.69995435895592
36-37	22.564369965737107	26.5140803692117	29.016461003365528	21.905088661685657
38-39	22.4159996693036	26.552316137885963	29.11228028619674	21.9194039066137
40-41	22.36217709415761	26.52237339054358	28.918685563264557	22.196763952034257
42-43	22.59110442317012	26.30573744063428	28.461754179500176	22.64140395669542
44-45	22.658539781256746	26.61066583502875	28.55751086554492	22.173283518169583
46-47	22.741399376852687	26.58985172463886	28.44765377543732	22.221095123071137
48-49	22.77394206533748	26.742405164701903	28.319853843849284	22.16379892611133
50-51	22.193904767353832	27.058885360762346	27.911838421048696	22.835371450835126
52-53	22.038768203475	27.34281920292817	27.446089185003718	23.17232340859311
54-55	22.288439931246966	26.934335286270205	27.452522322559243	23.32470245992359
56-57	22.812828746015843	26.487126984302993	27.6734848886982	23.02655938098296
58-59	22.60823618099124	26.644650011092097	28.09086109107506	22.656252716841607
60-61	22.454490648795808	27.163805319001792	27.980944849692307	22.40075918251009
62-63	22.07416589466435	26.927620181552374	27.995335638437442	23.002878285345833
64-65	21.76774929441912	27.291515562935242	27.678369548143472	23.26236559450217
66-67	22.42081849815784	26.589586197271576	27.58880387503762	23.400791429532962
68-69	22.29017050133363	26.540374728926828	27.72515066911193	23.444304100627612
70-71	22.400082413697735	26.36061832582741	27.710503514228996	23.528795746245862
72-73	22.49320729245686	26.218456781621764	28.11658949487741	23.17174643104397
74-75	22.290642422351105	27.04980039436229	27.554081773056843	23.105475410229758
76-77	22.27966079896861	26.63712738962502	27.559939443760285	23.523272367646086
78-79	22.113338949954333	26.718777886260803	27.680112437403547	23.48777072638132
80-81	22.065332102580147	26.734165185309806	27.627290175959736	23.57321253615031
82-83	22.186473382785493	26.426926572649297	27.94612648803314	23.44047355653207
84-85	22.39749532121347	26.679886621239312	27.240619248707908	23.681998808839314
86-87	21.89572288618277	26.902199623665528	27.478393706115035	23.723683784036663
88-89	22.183432806030275	26.527505498763905	27.211279955813044	24.077781739392776
90-91	22.216027549924725	26.14439827055131	27.56622975527035	24.07334442425362
92-93	22.206337838489542	26.635213574717937	27.339217364573837	23.819231222218686
94-95	21.602650170290946	26.553091689275565	27.719732471272117	24.124525669161372
96-97	21.75487553824072	26.303553824072175	27.6682681976625	24.273302440024604
98-99	21.879791452874805	26.63097053412315	27.064724146166707	24.424513866835337
100	22.037472165357865	26.16283009561091	27.023031508368383	24.776666230662837
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	525.0
1	330.0
2	390.5
3	679.5
4	476.0
5	341.0
6	699.0
7	1766.0
8	3248.5
9	3995.5
10	3287.0
11	2380.0
12	2470.5
13	2939.5
14	3442.5
15	4148.0
16	4968.5
17	5822.5
18	6909.0
19	8080.5
20	9583.5
21	11582.5
22	14003.5
23	17145.0
24	21304.0
25	26720.5
26	34910.5
27	45123.5
28	55442.0
29	69592.5
30	85448.0
31	100122.0
32	117876.5
33	134667.5
34	150266.5
35	167084.0
36	185703.0
37	208462.5
38	219598.0
39	216873.0
40	215817.5
41	216795.5
42	220597.5
43	233547.5
44	263707.5
45	294672.5
46	306433.5
47	323713.5
48	359829.5
49	364961.5
50	358508.0
51	341110.5
52	298414.5
53	273043.0
54	255431.0
55	231290.5
56	206211.0
57	179735.0
58	153811.5
59	135812.5
60	112821.0
61	86650.0
62	65492.0
63	49467.5
64	35617.0
65	24441.0
66	17071.0
67	11713.5
68	8592.5
69	6687.0
70	5157.5
71	4091.5
72	3287.0
73	2677.0
74	1931.5
75	1286.0
76	841.0
77	471.0
78	228.5
79	92.0
80	41.5
81	27.5
82	20.5
83	15.0
84	9.0
85	7.5
86	8.0
87	8.0
88	3.0
89	1.0
90	1.5
91	0.5
92	0.5
93	0.5
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0025195696417578775
2	0.003726863428433527
3	1.0498206840657822E-4
4	0.04999771007863288
5	0.0021915006779873207
6	0.00489478893945671
7	3.9368275652466835E-5
8	3.4119172232137927E-4
9	7.873655130493367E-5
10-11	1.1154344768198937E-4
12-13	0.0
14-15	2.6245517101644555E-5
16-17	3.4119172232137927E-4
18-19	1.2466620623281165E-4
20-21	2.0340275753774533E-4
22-23	0.0
24-25	0.0
26-27	2.296482746393899E-4
28-29	6.561379275411139E-6
30-31	2.296482746393899E-4
32-33	2.755779295672678E-4
34-35	1.9684137826233417E-4
36-37	7.217517202952252E-5
38-39	8.726634436296815E-4
40-41	4.6585792855419086E-4
42-43	0.0
44-45	5.970855140624136E-4
46-47	0.0
48-49	7.873655130493367E-5
50-51	0.0
52-53	4.199282736263129E-4
54-55	4.0680551507549066E-4
56-57	1.2466620623281165E-4
58-59	7.54558616672281E-4
60-61	6.167696518886471E-4
62-63	1.7715724043610074E-4
64-65	4.199282736263129E-4
66-67	0.0011482413731969494
68-69	0.0022833599878430764
70-71	0.0033659875682859144
72-73	0.002998550328862891
74-75	0.0026048675723382222
76-77	0.004264896529017241
78-79	0.0014303806820396283
80-81	0.0010891889597182491
82-83	7.217517202952253E-4
84-85	6.62699306816525E-4
86-87	0.0
88-89	0.0
90-91	1.9684137826233418E-5
92-93	8.529793058034482E-5
94-95	1.1154344768198937E-4
96-97	4.789806871050132E-4
98-99	1.1810482695740051E-4
100	0.07528526580606741
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7620349.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.475972920458986
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.31841798931411	34.29596171328362
2	14.969512359393613	14.812623762516688
3	6.035843361100899	8.958876680578808
4	3.156023211386577	6.245892757716092
5	1.8483041498828992	4.572332303418914
6	1.1241060438388029	3.3369744110815733
7	0.7821963349192468	2.7089947279462843
8	0.5387807071401575	2.1325359741225736
9	0.4035581754480231	1.79697900242666
>10	1.7449079705781336	14.216740648066873
>50	0.0551518941503721	1.844443875484647
>100	0.020702905730478345	1.8303802414514483
>500	0.0012474485819460783	0.4086853413184667
>1k	9.762640707381327E-4	0.8347860736355628
>5k	1.0847378563757023E-4	0.46640575865054207
>10k+	1.6271067845635535E-4	1.5373867283014127
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	20637	0.2708143682133194	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19101	0.25065781107925633	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17554	0.23035690360113428	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9228	0.12109681590698798	No Hit
CATGTAAGCAGTGGTATCAACGCAGAGTACATGTAAGCAGTGGTATCAAC	8153	0.10698985046485403	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.3122758550822279E-5	0.0
5	0.0	0.0	0.0	1.3122758550822279E-5	0.0
6	0.0	0.0	0.0	1.3122758550822279E-5	0.0
7	0.0	0.0	0.0	1.3122758550822279E-5	0.0
8	0.0	0.0	0.0	1.3122758550822279E-5	0.0
9	0.0	0.0	0.0	2.6245517101644558E-5	0.0
10-11	0.0	0.0	0.0	2.6245517101644558E-5	0.0
12-13	0.0	0.0	0.0	4.5929654927877976E-5	3.2806896377055695E-5
14-15	0.0	0.0	0.0	5.9052413478700256E-5	4.5929654927877976E-5
16-17	0.0	0.0	0.0	8.529793058034482E-5	5.2491034203289116E-5
18-19	0.0	0.0	0.0	1.7059586116068964E-4	5.2491034203289116E-5
20-21	0.0	0.0	0.0	2.2308689536397873E-4	5.9052413478700256E-5
22-23	0.0	0.0	0.0	3.6087586014761267E-4	7.873655130493367E-5
24-25	0.0	0.0	0.0	8.00488271600159E-4	7.873655130493367E-5
26-27	0.0	0.0	0.0	0.001837186197115119	1.0498206840657823E-4
28-29	0.0	0.0	0.0	0.005091630317719044	1.1810482695740051E-4
30-31	0.0	0.0	0.0	0.016652780600993473	1.1810482695740051E-4
32-33	0.0	0.0	0.0	0.04298359563321837	1.377889647836339E-4
34-35	0.0	0.0	0.0	0.08327702576351818	1.7059586116068964E-4
36-37	0.0	0.0	0.0	0.14672556335674392	1.7059586116068964E-4
38-39	0.0	0.0	0.0	0.24184587871237917	1.7059586116068964E-4
40-41	0.0	0.0	0.0	0.3499052339991252	1.7059586116068964E-4
42-43	0.0	0.0	0.0	0.4570328734287629	1.7059586116068964E-4
44-45	0.0	0.0	0.0	0.589717085136127	1.7715724043610077E-4
46-47	0.0	0.0	0.0	0.7517634691009558	1.837186197115119E-4
48-49	0.0	0.0	0.0	0.9277659067845843	1.9684137826233417E-4
50-51	0.0	0.0	0.0	1.1152573195794577	1.9684137826233417E-4
52-53	0.0	0.0	0.0	1.3052945475331903	2.0996413681315646E-4
54-55	0.0	0.0	0.0	1.5126538167740087	2.0996413681315646E-4
56-57	0.0	0.0	0.0	1.762019036136009	2.3620965391480103E-4
58-59	0.0	0.0	0.0	2.0436728029123077	2.3620965391480103E-4
60-61	0.0	0.0	0.0	2.337996593069425	2.3620965391480103E-4
62-63	0.0	0.0	0.0	2.639800355600511	2.493324124656233E-4
64-65	0.0	0.0	0.0	2.9621740421600116	2.755779295672678E-4
66-67	0.0	0.0	0.0	3.327222939526785	2.755779295672678E-4
68-69	0.0	0.0	0.0	3.743713050412783	2.755779295672678E-4
70-71	0.0	0.0	0.0	4.195739591454407	2.755779295672678E-4
72-73	0.0	0.0	0.0	4.645889578023263	2.82139308842679E-4
74-75	0.0	0.0	0.0	5.089911236348886	2.952620673935013E-4
76-77	0.0	0.0	0.0	5.572467875158999	3.2806896377055695E-4
78-79	0.0	0.0	0.0	6.114516539859263	3.2806896377055695E-4
80-81	0.0	0.0	0.0	6.698866416748105	3.2806896377055695E-4
82-83	0.0	0.0	0.0	7.3022442935356375	3.4119172232137927E-4
84-85	0.0	0.0	0.0	7.898608055877756	3.5431448087220154E-4
86-87	0.0	0.0	0.0	8.51183456295768	3.6087586014761267E-4
88	0.0	0.0	0.0	8.99866922105536	3.674372394230238E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	3005	0.0	24.096666	1
GAGTACT	21935	0.0	21.790508	12-13
CATGGGG	15665	0.0	21.400301	4
GCTATTG	5085	0.0	19.871412	8
GTATAAG	3110	0.0	19.805765	1
TAGGCAT	4255	0.0	19.772442	5
TATTCGC	575	0.0	19.61664	9
AGTACTT	23820	0.0	19.592577	12-13
CGACGAG	1470	0.0	19.502525	24-25
CTATTGA	5380	0.0	19.218594	9
GTGATCG	1595	0.0	19.15287	8
GTACTTT	25305	0.0	18.888552	14-15
GGGCTAT	5790	0.0	18.83249	6
CTAGGCA	4525	0.0	18.495335	4
ATTTAGG	5930	0.0	18.157728	1
CTAGTAC	3990	0.0	18.147158	3
GGCTATT	5765	0.0	17.772087	7
ACATGGG	40975	0.0	17.659582	3
TGATCGC	1770	0.0	17.524752	9
TAGTACT	4955	0.0	17.459627	4
>>END_MODULE