FastQCFastQC Report
Mon 16 Jan 2017
ERR1146634_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146634_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5750568
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT70577112.273065895403724No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT1707572.9693936320725185No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC1618982.8153392847454373No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC1272112.2121466957698788No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA1156292.0107405042423636No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA1128011.961562753453224No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG1104831.9212536918092264No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC876971.5250145724735364No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG575111.0000925126004943No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG291750.5073411878617903No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT263650.4584764496307147No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC216410.3763280427255186No Hit
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT190630.3314976885761546No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA186640.324559243539073No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA162580.28271989827787447No Hit
GAGTAGGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT80600.14016006766635922No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCA85500.034.0936663
GCTTATG17050.033.655911
GAGTACG87800.033.2141461
AGTACGC88500.032.938812
TACGCAG88900.032.7360044
CGCAGTG90750.032.0555426
ACGCAGT98850.029.5825375
TTATGTA20050.028.8428973
AGTAAGG20200.028.6649194
TATGTAC19950.028.5153854
GTACTAG5400.027.0018711
TCTCGGG4600.026.60800794
CTTATGT23800.023.9038522
GCATCGT6250.022.5573988
TAGGCAG37850.022.482754
AGTAGGC39550.022.3496552
GTAGGCA38750.022.082483
GTAATAC2901.2187229E-1021.0762583
TAGGCAT7800.020.493025
GAGTAGG44100.020.3713911
Produced by FastQC (version 0.11.5)