##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146634_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5750568
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.962953398690356	33.0	31.0	34.0	30.0	34.0
2	31.99076595564125	33.0	31.0	34.0	30.0	34.0
3	32.14290414442539	34.0	31.0	34.0	30.0	34.0
4	35.339118848781546	37.0	35.0	37.0	33.0	37.0
5	35.507356490697966	37.0	35.0	37.0	33.0	37.0
6	35.75306091502613	37.0	35.0	37.0	35.0	37.0
7	35.781045454988096	37.0	36.0	37.0	35.0	37.0
8	35.787342050385284	37.0	37.0	37.0	35.0	37.0
9	37.480135527481806	39.0	38.0	39.0	35.0	39.0
10-11	37.43956666541462	39.0	38.0	39.0	35.0	39.0
12-13	37.468511719190175	39.0	38.0	39.0	35.0	39.0
14-15	38.55389545867469	40.0	38.0	41.0	35.0	41.0
16-17	38.837641864247146	40.5	39.0	41.0	36.0	41.0
18-19	38.987238391059805	41.0	39.0	41.0	36.0	41.0
20-21	38.94510977002619	41.0	39.0	41.0	36.0	41.0
22-23	38.86148698702459	40.5	39.0	41.0	36.0	41.0
24-25	38.73081224324275	40.5	39.0	41.0	35.0	41.0
26-27	38.42197622913076	40.0	38.5	41.0	34.5	41.0
28-29	38.51116367983128	40.0	38.0	41.0	34.5	41.0
30-31	38.67382752451584	40.0	38.5	41.0	35.0	41.0
32-33	38.45700894242099	40.0	38.0	41.0	34.0	41.0
34-35	38.06212594999312	40.0	38.0	41.0	33.0	41.0
36-37	37.84124629080118	40.0	37.5	41.0	32.5	41.0
38-39	37.66430959515651	40.0	37.5	41.0	32.5	41.0
40-41	37.98524684865912	40.0	38.0	41.0	33.0	41.0
42-43	37.77067013206347	40.0	38.0	41.0	33.0	41.0
44-45	37.483929935269	40.0	37.0	41.0	31.5	41.0
46-47	37.14255330951656	39.0	37.0	41.0	31.0	41.0
48-49	36.808268765798445	39.0	36.0	41.0	30.0	41.0
50-51	36.342324184324056	39.0	35.0	40.0	28.5	41.0
52-53	36.62244233960889	39.0	36.0	40.0	29.5	41.0
54-55	36.42846037121898	39.0	35.5	40.0	28.5	41.0
56-57	35.61982929338458	38.0	34.0	40.0	27.0	41.0
58-59	35.312376186143695	38.0	34.0	40.0	26.5	41.0
60-61	34.52122068637394	37.0	32.0	39.5	25.0	41.0
62-63	34.776151068903104	37.0	32.5	40.0	26.0	41.0
64-65	34.59643664764941	36.5	33.0	39.0	26.0	40.5
66-67	33.894803348121435	36.0	32.0	39.0	25.5	39.5
68-69	33.150642336548316	35.5	31.5	37.5	24.5	39.0
70-71	32.32477965654871	34.5	30.5	37.0	23.0	38.0
72-73	31.781597748257216	34.0	30.0	36.0	23.0	37.0
74-75	31.73713283626939	34.0	30.5	36.0	23.0	37.0
76-77	31.671265777571886	34.0	31.0	35.0	24.0	36.5
78-79	31.111311873887935	34.0	30.0	35.0	21.0	36.0
80-81	30.37427311528183	34.0	29.0	35.0	20.0	36.0
82-83	30.003208813459818	33.5	28.5	35.0	19.5	35.0
84-85	30.05181844645607	33.0	29.0	35.0	20.0	35.0
86-87	30.596742791320786	34.0	29.0	35.0	20.0	35.0
88-89	30.38405110590815	34.0	29.0	35.0	20.0	35.0
90-91	29.92524738425839	33.5	29.0	35.0	19.0	35.0
92-93	29.580139648813823	33.0	27.5	35.0	18.0	35.0
94-95	29.55310484112178	33.0	28.0	35.0	18.5	35.0
96-97	29.30928701303941	33.0	27.5	35.0	17.5	35.0
98-99	29.786195207151714	34.0	29.0	35.0	18.5	35.0
100	28.121793360238502	32.0	25.0	34.0	10.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.25596374816992196
1101	2	0.2908862215009762
1101	3	0.27825483131644546
1101	4	0.24987635114111129
1101	5	0.21598444334784972
1101	6	0.2341062548684789
1101	7	0.25237641352993734
1101	8	0.2322819076993312
1101	9	0.3588218513800072
1101	10-11	0.3509225609974109
1101	12-13	0.3567059418957612
1101	14-15	0.486492940927576
1101	16-17	0.4450079167516918
1101	18-19	0.38388606453299445
1101	20-21	0.4305608842111184
1101	22-23	0.4934377895011437
1101	24-25	0.5338714982146726
1101	26-27	0.5249682535840776
1101	28-29	0.4955644149021623
1101	30-31	0.5120739737436182
1101	32-33	0.5486563252219057
1101	34-35	0.5418902751851107
1101	36-37	0.6083174556230873
1101	38-39	0.6528838025667838
1101	40-41	0.574170965558757
1101	42-43	0.6116461280770906
1101	44-45	0.6258273628525259
1101	46-47	0.6600362964581024
1101	48-49	0.7236265775913893
1101	50-51	0.7067271210914257
1101	52-53	0.6154038164414146
1101	54-55	0.6224317878895178
1101	56-57	0.6652831527554142
1101	58-59	0.8028721227040876
1101	60-61	0.9021326553496181
1101	62-63	0.8364983601306051
1101	64-65	0.7654264561970763
1101	66-67	0.671178768547442
1101	68-69	0.7952824207519953
1101	70-71	0.8072874648059312
1101	72-73	0.7734515196436185
1101	74-75	0.7390532306781914
1101	76-77	0.6651253247571915
1101	78-79	0.7607533940702602
1101	80-81	0.8260352578980701
1101	82-83	0.8234544341249475
1101	84-85	0.8246381278447714
1101	86-87	0.698277121478764
1101	88-89	0.6935950697243989
1101	90-91	0.7866133311344896
1101	92-93	0.8762533105466552
1101	94-95	0.8456901174473117
1101	96-97	0.808568223652621
1101	98-99	0.7867279681575425
1101	100	0.814247362733866
1102	1	0.2526918964757634
1102	2	0.3178216710361852
1102	3	0.2949242137579944
1102	4	0.2409859116323716
1102	5	0.21641940535766224
1102	6	0.28575613191396343
1102	7	0.20779696879201026
1102	8	0.26748097837868556
1102	9	0.313448261502316
1102	10-11	0.33351575806892697
1102	12-13	0.3227167051248685
1102	14-15	0.44262649660402786
1102	16-17	0.4070884718911145
1102	18-19	0.36530096572697346
1102	20-21	0.42178520809267894
1102	22-23	0.40705599885935584
1102	24-25	0.4832368610465778
1102	26-27	0.5070307794824984
1102	28-29	0.5042934410165287
1102	30-31	0.43500608637994276
1102	32-33	0.4703300923258098
1102	34-35	0.4555666327878356
1102	36-37	0.48626012911948635
1102	38-39	0.5203346054286797
1102	40-41	0.4913654562151848
1102	42-43	0.5102972421142269
1102	44-45	0.5323303515189792
1102	46-47	0.6068082037818456
1102	48-49	0.6357554910449039
1102	50-51	0.6699607854864738
1102	52-53	0.640362335170181
1102	54-55	0.5435935000191279
1102	56-57	0.6273717019096239
1102	58-59	0.6893621501158975
1102	60-61	0.6795190549241994
1102	62-63	0.6113463862440938
1102	64-65	0.712121270556338
1102	66-67	0.6974567377435434
1102	68-69	0.6574698766754068
1102	70-71	0.6153244501556401
1102	72-73	0.6777088471984847
1102	74-75	0.6995439624393462
1102	76-77	0.6095536716650187
1102	78-79	0.5985898763883739
1102	80-81	0.5613619699970229
1102	82-83	0.5102048036017415
1102	84-85	0.5376402943322844
1102	86-87	0.5170880676284533
1102	88-89	0.5104375171106419
1102	90-91	0.6486360884519655
1102	92-93	0.6355015095936807
1102	94-95	0.5917216913965504
1102	96-97	0.643359095845625
1102	98-99	0.6731607233812511
1102	100	0.750052357832331
1103	1	0.15477514597065678
1103	2	0.1887743503849748
1103	3	0.1809249083128961
1103	4	0.2182479153274386
1103	5	0.1626472398049046
1103	6	0.18359667726944195
1103	7	0.20191512999731032
1103	8	0.24587376301364827
1103	9	0.27294748145882153
1103	10-11	0.24378150622825956
1103	12-13	0.2776955959364571
1103	14-15	0.35063504780551114
1103	16-17	0.37697335825303924
1103	18-19	0.3692772765502923
1103	20-21	0.3539860464557876
1103	22-23	0.4006160089774653
1103	24-25	0.45481294174270204
1103	26-27	0.42530275265930584
1103	28-29	0.41538197696421264
1103	30-31	0.4907152126652292
1103	32-33	0.4592243202594801
1103	34-35	0.5034171163253731
1103	36-37	0.49740002210012335
1103	38-39	0.5575962673822943
1103	40-41	0.494479322337277
1103	42-43	0.509824606235803
1103	44-45	0.5580279246010846
1103	46-47	0.632173377410993
1103	48-49	0.6421696107796109
1103	50-51	0.7239757897459995
1103	52-53	0.6311119210881344
1103	54-55	0.6002255411439421
1103	56-57	0.6419030594024804
1103	58-59	0.7621143537094781
1103	60-61	0.7637770064812344
1103	62-63	0.7552502776891217
1103	64-65	0.7025134917969922
1103	66-67	0.7557529585941154
1103	68-69	0.6677667009498194
1103	70-71	0.7164788282894676
1103	72-73	0.6397982757194818
1103	74-75	0.6571998013315685
1103	76-77	0.6232260444557625
1103	78-79	0.6029322285021586
1103	80-81	0.5965395147944292
1103	82-83	0.7107233829452682
1103	84-85	0.7371719754681365
1103	86-87	0.6467480210451519
1103	88-89	0.5443321474688254
1103	90-91	0.6872472032892318
1103	92-93	0.6049969863634601
1103	94-95	0.6482000553301148
1103	96-97	0.7288246935535518
1103	98-99	0.6490869544968412
1103	100	0.7730331396132719
1104	1	0.17359651656051156
1104	2	0.24159513821770418
1104	3	0.19267546122503632
1104	4	0.20154923029539162
1104	5	0.13163580470412484
1104	6	0.2023909066012166
1104	7	0.20595111826563084
1104	8	0.23556528441739033
1104	9	0.2641851706625076
1104	10-11	0.2751467915816761
1104	12-13	0.3005049636428865
1104	14-15	0.39840397399751737
1104	16-17	0.38696105525593794
1104	18-19	0.37705264905106617
1104	20-21	0.34748405131334437
1104	22-23	0.35317184197698737
1104	24-25	0.4331374234902512
1104	26-27	0.47162086941788317
1104	28-29	0.3725092401395784
1104	30-31	0.3954151582579257
1104	32-33	0.3730532176089554
1104	34-35	0.4142267566922655
1104	36-37	0.4744631632733416
1104	38-39	0.41981497060002226
1104	40-41	0.3800360758518124
1104	42-43	0.4217306598386372
1104	44-45	0.4525414232693876
1104	46-47	0.5375985197414082
1104	48-49	0.5369260289072244
1104	50-51	0.6179621396689825
1104	52-53	0.5232059221837702
1104	54-55	0.5375853373078883
1104	56-57	0.5941802265539451
1104	58-59	0.6902496057737793
1104	60-61	0.6580154789337911
1104	62-63	0.5777928551477771
1104	64-65	0.6012155833169004
1104	66-67	0.5962257686689512
1104	68-69	0.577574948481427
1104	70-71	0.6081619408946963
1104	72-73	0.6989249865026501
1104	74-75	0.5478488424028463
1104	76-77	0.5141025105516519
1104	78-79	0.5436536930569211
1104	80-81	0.5178080545614705
1104	82-83	0.6200261635411621
1104	84-85	0.6166210880009899
1104	86-87	0.5053940351902213
1104	88-89	0.5404577848724301
1104	90-91	0.5966331494834023
1104	92-93	0.6610124354587299
1104	94-95	0.7306847563956751
1104	96-97	0.6156851373640748
1104	98-99	0.5281654744684374
1104	100	0.5834284182406435
1105	1	0.07332075639768831
1105	2	0.08913909654586405
1105	3	0.0839775884267766
1105	4	0.08095773222663638
1105	5	0.03855578364410661
1105	6	0.06646161426997566
1105	7	0.10922632327928739
1105	8	0.10772926478599487
1105	9	0.11986540665184009
1105	10-11	0.15024765906591142
1105	12-13	0.1639393188454079
1105	14-15	0.2839920278495356
1105	16-17	0.2878241068956271
1105	18-19	0.21650584144526874
1105	20-21	0.2173820904016992
1105	22-23	0.21322714043651558
1105	24-25	0.25986777633466573
1105	26-27	0.25135819536874493
1105	28-29	0.2012236146195221
1105	30-31	0.28291963064656755
1105	32-33	0.2945197918356399
1105	34-35	0.267488106850692
1105	36-37	0.27075502785514516
1105	38-39	0.2983748168899112
1105	40-41	0.27584142094513453
1105	42-43	0.3219208477680908
1105	44-45	0.34935847519812313
1105	46-47	0.4202264093660375
1105	48-49	0.40833335592127895
1105	50-51	0.3504475654117343
1105	52-53	0.32412390780719136
1105	54-55	0.28724102704571663
1105	56-57	0.36273974019998434
1105	58-59	0.38701538439276817
1105	60-61	0.4046415312184024
1105	62-63	0.3820660951753041
1105	64-65	0.4716679200683416
1105	66-67	0.4661333175516802
1105	68-69	0.48151357903284264
1105	70-71	0.4357712454366691
1105	72-73	0.45633602098365245
1105	74-75	0.4545060406566783
1105	76-77	0.43242697709595035
1105	78-79	0.44628114976706357
1105	80-81	0.38337632008320455
1105	82-83	0.34646955933514434
1105	84-85	0.36955482375380555
1105	86-87	0.3688544050519873
1105	88-89	0.41132840933248005
1105	90-91	0.3710159121017469
1105	92-93	0.5239760120709072
1105	94-95	0.45372821368897576
1105	96-97	0.459815875311385
1105	98-99	0.43614606207346185
1105	100	0.5082191550165405
1106	1	0.07314807941831347
1106	2	0.11200374702466576
1106	3	0.1516533125928774
1106	4	0.1188152107558409
1106	5	0.09542685412295526
1106	6	0.1258813987297387
1106	7	0.111529026259511
1106	8	0.12575401696738453
1106	9	0.1776969689231933
1106	10-11	0.15268820352651602
1106	12-13	0.18039749598229804
1106	14-15	0.23676551391574208
1106	16-17	0.19753625284107557
1106	18-19	0.20666307708954434
1106	20-21	0.20491465949694998
1106	22-23	0.2737570195633623
1106	24-25	0.27790272319545295
1106	26-27	0.2877244041403344
1106	28-29	0.205355755023362
1106	30-31	0.263359336142436
1106	32-33	0.25159466763672356
1106	34-35	0.2993159694684877
1106	36-37	0.27060606490633177
1106	38-39	0.2720346633945283
1106	40-41	0.2466956857965883
1106	42-43	0.31212974822337713
1106	44-45	0.31997500395097944
1106	46-47	0.42733999332028816
1106	48-49	0.3504742474202516
1106	50-51	0.3610460600268297
1106	52-53	0.3177316378750277
1106	54-55	0.36624306943866713
1106	56-57	0.37895996792970266
1106	58-59	0.3704885880898843
1106	60-61	0.41581264905911297
1106	62-63	0.34742789927166484
1106	64-65	0.4252942212231403
1106	66-67	0.48244467218111
1106	68-69	0.3783612900147091
1106	70-71	0.3956313576649393
1106	72-73	0.337758806915911
1106	74-75	0.3114207658412873
1106	76-77	0.3611457640732709
1106	78-79	0.2727978345073403
1106	80-81	0.25752142891361274
1106	82-83	0.26379380611493985
1106	84-85	0.27302520000882424
1106	86-87	0.28538755647968017
1106	88-89	0.29497638631751144
1106	90-91	0.33639967227169265
1106	92-93	0.33906388013027566
1106	94-95	0.2529861278237391
1106	96-97	0.266351525391741
1106	98-99	0.2619439042290779
1106	100	0.30567742873073556
1107	1	0.04372836738207653
1107	2	0.10830284972515614
1107	3	0.11520051446340318
1107	4	0.07435257471215095
1107	5	0.056939782656819204
1107	6	0.09614950391623012
1107	7	0.05872066237844109
1107	8	0.07011533325428587
1107	9	0.11929226774683599
1107	10-11	0.09065480012894511
1107	12-13	0.10924033800423416
1107	14-15	0.14929871008616402
1107	16-17	0.13086019520421388
1107	18-19	0.13642937338017447
1107	20-21	0.18069874578074518
1107	22-23	0.1921445242086719
1107	24-25	0.22868008689292196
1107	26-27	0.228379266312011
1107	28-29	0.22913939780652015
1107	30-31	0.23162723431940435
1107	32-33	0.21659753033987528
1107	34-35	0.22797712683046
1107	36-37	0.25068527230883575
1107	38-39	0.26006012452099725
1107	40-41	0.23857660494787325
1107	42-43	0.26969664843976915
1107	44-45	0.2850173011844461
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2216	96-97	-2.6062271366939527
2216	98-99	-2.655874529606379
2216	100	-2.8511893129720107
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1773.0
1	463.0
2	348.0
3	348.0
4	280.0
5	343.0
6	597.0
7	1667.0
8	3941.0
9	4546.0
10	4395.0
11	4949.0
12	5995.0
13	6983.0
14	8329.0
15	8978.0
16	9953.0
17	11248.0
18	13002.0
19	15429.0
20	18456.0
21	22712.0
22	27813.0
23	34077.0
24	41815.0
25	49818.0
26	59587.0
27	69558.0
28	83953.0
29	103665.0
30	130220.0
31	166130.0
32	221560.0
33	310420.0
34	461535.0
35	714567.0
36	1063013.0
37	1274061.0
38	710608.0
39	83331.0
40	102.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	55.59358369939918	15.216067627663508	12.782452094634724	16.407896578302584
2	12.202707946523189	30.944944917075446	26.48071358097303	30.371633555428335
3	40.74686365924174	18.479488216575806	28.679043792861236	12.094604331321221
4	9.865223837658068	12.366081428200221	49.601435774517824	28.167258959623886
5	9.26632701597906	45.831035240745834	33.58103711170792	11.321600631567186
6	24.462899506322355	38.30958007549545	13.144273866728446	24.083246551453747
7	24.263084646665632	33.662789038116514	22.77087413383423	19.303252181383623
8	20.991599976398266	15.08714626338106	19.503350627926174	44.4179031322945
9	14.058061206234646	30.576769530953605	32.383455396355025	22.98171386645672
10-11	29.21049297328756	17.560176254327516	25.78630642132985	27.44302435105507
12-13	40.64122652564798	19.193881780836556	20.624922342386803	19.539969351128658
14-15	25.207971731960427	24.168126125050538	36.68135088036537	13.94255126262367
16-17	17.939008453793825	27.266959386685514	32.25980324459926	22.534228914921403
18-19	15.548372527921494	41.73262284304311	22.345462052917807	20.37354257611759
20-21	24.20174066004747	24.597571450063636	17.48559956778908	33.71508832209981
22-23	18.15480673370578	26.81451435712734	24.080896637054142	30.949782272112742
24-25	32.206584437770715	24.99018432583026	19.78274293097423	23.020488305424795
26-27	26.628485310445406	16.347763588838653	37.41127272347579	19.61247837724015
28-29	17.001108123588413	40.13852676126298	23.613013725451633	19.247351389696973
30-31	22.06214222186639	22.994484407878822	22.32205732108265	32.62131604917214
32-33	25.439188192504908	25.357662123352043	26.728584590650456	22.474565093492597
34-35	33.02752237688373	20.731521692494724	24.850413918718015	21.39054201190353
36-37	25.842884375187108	17.78365758836135	36.66930825903456	19.70414977741698
38-39	21.261736737816978	29.169770702818766	30.66716644549684	18.901326113867416
40-41	20.590454959538302	32.65592294681307	24.67812701355546	22.075495080093166
42-43	17.765390054454095	31.506804607636628	16.491525103633705	34.236280234275576
44-45	24.515022216698906	20.309823238500833	20.905183948627588	34.26997059617267
46-47	32.837162365963266	28.601193309654647	23.723708487967958	14.837935836414132
48-49	27.364135938170016	25.364746732342564	26.018926612357802	21.252190717129626
50-51	11.709257181846109	28.70190236570781	39.321957489841544	20.266882962604534
52-53	22.923553949180558	34.331992198977595	19.69736398690453	23.047089864937316
54-55	17.024504906551	22.85612962066913	27.114438035430204	33.004927437349664
56-57	28.5592203753214	17.660145976405968	25.73870061451153	28.0419330337611
58-59	39.19117245153706	19.816533436570555	20.946558026200854	20.045736085691537
60-61	24.856222223808196	24.377067412059212	36.49945002465707	14.267260339475524
62-63	18.08584739380308	26.711163019461022	32.18845133213589	23.014538254600012
64-65	15.72319314644727	40.54381292059852	22.624703615073596	21.108290317880613
66-67	23.881544704266496	24.40787148818093	17.991332624252983	33.71925118329959
68-69	18.153917309876768	26.386905051509757	24.42683358723454	31.032344051378942
70-71	31.43644794054068	24.814146099113493	20.2581274198956	23.491278540450228
72-73	26.102560616648702	16.602583289604848	36.87748048015141	20.417375613595034
74-75	17.226554703855925	38.87731216837454	24.02244989208423	19.87368323568531
76-77	22.013008489549517	22.81337090993279	22.843076499945166	32.33054410057253
78-79	25.080866965594307	24.86488806055243	26.960213331067905	23.094031642785357
80-81	32.20570578085119	20.67078501813656	25.07409333527718	22.04941586573507
82-83	25.33227350499663	18.021050857746335	35.98036253684396	20.666313100413078
84-85	21.21324058685895	28.48539545924319	30.40007144163972	19.90129251225814
86-87	20.593265966422614	31.69983283894068	25.167940030208403	22.538961164428308
88-89	18.15641500227945	30.78827539375996	17.531032809283236	33.52427679467735
90-91	24.340690534771614	20.3556637813514	21.452102730835882	33.851542953041104
92-93	31.60304289844876	27.75801074112622	24.233259406753337	16.40568695367168
94-95	26.68877062052251	24.96067101052266	26.18600531998776	22.164553048967072
96-97	12.921637709463926	27.76459051301936	38.06789160715282	21.245880170363893
98-99	23.04923330306335	32.98760038990919	20.664682188236938	23.298484118790523
100	21.31656780492771	14.438346430639712	21.73488667073201	42.51019909370057
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1643.0
1	1033.5
2	381.5
3	337.0
4	317.0
5	296.5
6	274.5
7	238.0
8	231.0
9	246.5
10	248.0
11	248.5
12	267.0
13	270.5
14	306.5
15	363.0
16	360.0
17	384.5
18	481.5
19	552.5
20	612.0
21	813.0
22	1139.0
23	1502.5
24	2026.0
25	2853.5
26	3959.5
27	5232.0
28	6629.0
29	8453.5
30	10449.0
31	12502.0
32	14907.5
33	17237.5
34	19451.0
35	21723.5
36	24089.5
37	26932.5
38	28969.5
39	29853.5
40	30570.5
41	31190.0
42	32293.0
43	34848.0
44	38797.0
45	50105.0
46	110276.5
47	571916.0
48	1591353.5
49	1637159.5
50	708698.5
51	234038.0
52	96406.0
53	60066.5
54	46783.0
55	37940.0
56	33052.0
57	28974.0
58	25028.5
59	22375.0
60	18710.5
61	14651.5
62	11544.5
63	9196.0
64	6851.5
65	4733.0
66	3551.5
67	2806.0
68	2200.5
69	1678.5
70	1264.5
71	1015.0
72	805.0
73	613.0
74	480.0
75	345.0
76	242.0
77	183.0
78	137.0
79	103.0
80	80.5
81	67.5
82	53.0
83	37.5
84	31.0
85	37.0
86	34.5
87	26.0
88	28.5
89	27.5
90	21.5
91	16.5
92	19.0
93	21.0
94	12.5
95	8.5
96	10.5
97	11.0
98	13.0
99	12.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09912064338687934
2	0.06569785802028599
3	0.0817310568277777
4	0.08380041762831081
5	0.08453078026379307
6	0.09252999008097983
7	0.08924335822130962
8	0.09089536894442427
9	0.07574903905144674
10-11	0.08312222375250584
12-13	0.07286236768263588
14-15	0.08199190062616424
16-17	0.08354826862320383
18-19	0.08295702268019438
20-21	0.0747665274108575
22-23	0.07859223645385986
24-25	0.08096591501917723
26-27	0.07491433889660987
28-29	0.08380041762831081
30-31	0.08760873708475406
32-33	0.08984329895759863
34-35	0.09212133479684094
36-37	0.09209525041700228
38-39	0.09320818395678479
40-41	0.08927813739442782
42-43	0.0948341103000608
44-45	0.09090406373770382
46-47	0.08755656832507676
48-49	0.09718170448553952
50-51	0.09308645685087108
52-53	0.09877285165569732
54-55	0.09021717506861932
56-57	0.09735560035113053
58-59	0.10207687310192662
60-61	0.10025096651322095
62-63	0.10133781567316481
64-65	0.10306807953579541
66-67	0.10499832364385571
68-69	0.10224207417423808
70-71	0.10437229852772806
72-73	0.10152910112531492
74-75	0.10505918719681255
76-77	0.1018681980632174
78-79	0.10880664310029896
80-81	0.10356368275272981
82-83	0.1085371045086329
84-85	0.10466792149923278
86-87	0.10101610832182142
88-89	0.10018140816698455
90-91	0.10065962179735984
92-93	0.0948341103000608
94-95	0.09463413005463112
96-97	0.0927995286726459
98-99	0.10700682089143194
100	0.2482189585446168
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5750568.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.551003034956544
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.4126373478051	40.42250105895648
2	13.90959371969478	14.341070161179962
3	4.2402438240463285	6.557664667271039
4	1.6682406525570428	3.43997915772024
5	0.7803640676731188	2.011427521049399
6	0.4033479256861973	1.247579408471555
7	0.23047126926355124	0.8316717570892931
8	0.11336278595493487	0.4675172258251182
9	0.07200844196606151	0.3340896669301436
>10	0.16786582684851864	1.1517177551155755
>50	4.864187813456848E-4	0.015884946527679668
>100	3.8335935737825985E-4	0.05047098362147707
>500	1.553688325976079E-4	0.06093565910077913
>1k	3.1073760481850735E-4	0.32779342488210783
>5k	6.214752096324868E-5	0.2113685305821915
>10k+	4.6610640722436516E-4	28.528328075677063
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	705771	12.273065895403724	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	170757	2.9693936320725185	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	161898	2.8153392847454373	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	127211	2.2121466957698788	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	115629	2.0107405042423636	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	112801	1.961562753453224	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	110483	1.9212536918092264	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	87697	1.5250145724735364	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	57511	1.0000925126004943	No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG	29175	0.5073411878617903	No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	26365	0.4584764496307147	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC	21641	0.3763280427255186	No Hit
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	19063	0.3314976885761546	No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA	18664	0.324559243539073	No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA	16258	0.28271989827787447	No Hit
GAGTAGGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	8060	0.14016006766635922	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.738958655910164E-5	0.0
8	0.0	0.0	0.0	3.477917311820328E-5	0.0
9	0.0	0.0	0.0	1.0433751935460984E-4	0.0
10-11	0.0	0.0	0.0	4.956032169343967E-4	0.0
12-13	0.0	0.0	0.0	8.260053615573279E-4	0.0
14-15	0.0	0.0	0.0	0.0015215888239213935	0.0
16-17	0.0	0.0	0.0	0.0029649245083268297	0.0
18-19	0.0	0.0	0.0	0.004808220683591604	0.0
20-21	0.0	0.0	0.0	0.008886078731700938	0.0
22-23	0.0	0.0	0.0	0.014424662050774811	0.0
24-25	0.0	0.0	0.0	0.018789448277109322	0.0
26-27	0.0	0.0	0.0	0.02255429376715483	0.0
28-29	0.0	0.0	0.0	0.03285762380342255	0.0
30-31	0.0	0.0	0.0	0.04445647803834334	0.0
32-33	0.0	0.0	0.0	0.08899990400948218	1.738958655910164E-5
34-35	0.0	0.0	0.0	0.13519534070373568	1.738958655910164E-5
36-37	0.0	0.0	0.0	0.17454797508698272	1.738958655910164E-5
38-39	0.0	0.0	0.0	0.23689833769464164	3.477917311820328E-5
40-41	0.0	0.0	0.0	0.29986603062514866	3.477917311820328E-5
42-43	0.0	0.0	0.0	0.3812406009284648	3.477917311820328E-5
44-45	0.0	0.0	0.0	0.48662149547662076	3.477917311820328E-5
46-47	0.0	0.0	0.0	0.6277988539566874	3.477917311820328E-5
48-49	0.0	0.0	0.0	0.7167813683796105	3.477917311820328E-5
50-51	0.0	0.0	0.0	0.828579020368075	3.477917311820328E-5
52-53	0.0	0.0	0.0	0.9428199092680931	3.477917311820328E-5
54-55	0.0	0.0	0.0	1.108873071320955	3.477917311820328E-5
56-57	0.0	0.0	0.0	1.4029048956555248	3.477917311820328E-5
58-59	0.0	0.0	0.0	1.58979078240619	3.477917311820328E-5
60-61	0.0	0.0	0.0	1.7760680336272867	3.477917311820328E-5
62-63	0.0	0.0	0.0	1.9619453243575244	3.477917311820328E-5
64-65	0.0	0.0	0.0	2.145440241729165	3.477917311820328E-5
66-67	0.0	0.0	0.0	2.412987725734223	3.477917311820328E-5
68-69	0.0	0.0	0.0	2.7369035545706093	3.477917311820328E-5
70-71	0.0	0.0	0.0	3.0185539932750993	3.477917311820328E-5
72-73	0.0	0.0	0.0	3.231872051595599	3.477917311820328E-5
74-75	0.0	0.0	0.0	3.544241194956742	3.477917311820328E-5
76-77	0.0	0.0	0.0	3.7946512414078053	3.477917311820328E-5
78-79	0.0	0.0	0.0	4.3971308573344405	3.477917311820328E-5
80-81	0.0	0.0	0.0	4.949763223389411	3.477917311820328E-5
82-83	0.0	0.0	0.0	5.268331754358874	3.477917311820328E-5
84-85	0.0	0.0	0.0	5.636556249747851	3.477917311820328E-5
86-87	0.0	0.0	0.0	5.984939226872894	3.477917311820328E-5
88	0.0	0.0	0.0	6.330470311802243	3.477917311820328E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACGCA	8550	0.0	34.093666	3
GCTTATG	1705	0.0	33.65591	1
GAGTACG	8780	0.0	33.214146	1
AGTACGC	8850	0.0	32.93881	2
TACGCAG	8890	0.0	32.736004	4
CGCAGTG	9075	0.0	32.055542	6
ACGCAGT	9885	0.0	29.582537	5
TTATGTA	2005	0.0	28.842897	3
AGTAAGG	2020	0.0	28.66491	94
TATGTAC	1995	0.0	28.515385	4
GTACTAG	540	0.0	27.001871	1
TCTCGGG	460	0.0	26.608007	94
CTTATGT	2380	0.0	23.903852	2
GCATCGT	625	0.0	22.557398	8
TAGGCAG	3785	0.0	22.48275	4
AGTAGGC	3955	0.0	22.349655	2
GTAGGCA	3875	0.0	22.08248	3
GTAATAC	290	1.2187229E-10	21.076258	3
TAGGCAT	780	0.0	20.49302	5
GAGTAGG	4410	0.0	20.371391	1
>>END_MODULE