FastQCFastQC Report
Mon 16 Jan 2017
ERR1146634_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146634_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5750568
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT72655012.634404114515297No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC1762893.065592824917469No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT1709122.9720890179891795No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC1429542.485910956969816No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA1234702.1470922524522797No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA1222472.1258247880904984No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG1217722.117564734474925No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC974411.6944587039054229No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG635871.1057516405335959No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT594311.0334805187939695No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA337940.5876636881782809No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG331310.5761343922895964No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT282780.49174272871827623No Hit
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT220070.3826926314061498No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA215060.37398044854003987No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC190650.3315324677492728No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA181540.3156905543939312No Hit
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT177920.30939552405953635No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG175550.3052741920450293No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC123880.21542219829415113No Hit
GAGTAGGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT83740.14562039784591715No Hit
AGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG61250.10651121767449755No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCA95500.032.848493
TACGCAG96850.032.4386144
GAGTACG97050.032.2756841
AGTACGC98400.031.8806742
CGCAGTG100400.031.2257696
GCTTATG17300.029.9049681
GTACTAG3800.029.7046471
ACGCAGT107500.029.20795
TATGTAC17950.026.4632024
AGTAAGG8450.025.60430594
TTAGGTA4350.024.8674683
GTACCGT1151.3776463E-424.5230466
TTATGTA20200.023.7488083
CTTAGGT4550.022.740922
TTCGTAT1050.002327299722.396361
CCCGTAT1503.2768767E-521.9484351
CTTATGT24200.021.3783452
GCTTAGG5100.021.2106721
GTGATCG2901.2187229E-1021.0677078
GTAGGCA43850.021.0222633
Produced by FastQC (version 0.11.5)