##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146634_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5750568
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.35456306229228	33.0	31.0	34.0	30.0	34.0
2	32.30191261106729	33.0	31.0	34.0	30.0	34.0
3	32.50744291694316	34.0	31.0	34.0	30.0	34.0
4	35.74110435699569	37.0	35.0	37.0	35.0	37.0
5	35.86411498829333	37.0	35.0	37.0	35.0	37.0
6	36.11170218315826	37.0	35.0	37.0	35.0	37.0
7	36.15409312610511	37.0	36.0	37.0	35.0	37.0
8	36.18407033879088	37.0	37.0	37.0	35.0	37.0
9	37.922998910716295	39.0	38.0	39.0	35.0	39.0
10-11	37.870023448118516	39.0	38.0	39.0	35.0	39.0
12-13	37.90256292943584	39.0	38.0	39.0	35.0	39.0
14-15	38.882345013570834	40.0	38.0	41.0	35.5	41.0
16-17	39.13986140151721	40.0	38.5	41.0	36.0	41.0
18-19	39.35167856462179	41.0	39.0	41.0	36.0	41.0
20-21	39.36874626993368	41.0	39.0	41.0	36.0	41.0
22-23	39.2772990424598	40.0	39.0	41.0	36.0	41.0
24-25	39.133234577871264	40.0	39.0	41.0	36.0	41.0
26-27	38.86027936718599	40.0	38.5	41.0	35.0	41.0
28-29	38.974157074570726	40.0	38.5	41.0	36.0	41.0
30-31	39.12885309764184	40.0	39.0	41.0	36.0	41.0
32-33	39.00191685412641	40.0	38.0	41.0	35.0	41.0
34-35	38.70522616200695	40.0	38.0	41.0	35.0	41.0
36-37	38.509034933592645	40.0	38.0	41.0	34.0	41.0
38-39	38.44218449377522	40.0	38.0	41.0	35.0	41.0
40-41	38.79322025232986	40.0	38.0	41.0	35.0	41.0
42-43	38.728408915432354	40.0	38.0	41.0	35.0	41.0
44-45	38.5854083457495	40.0	38.0	41.0	35.0	41.0
46-47	38.38300129656757	40.0	38.0	41.0	34.0	41.0
48-49	38.022338228154155	40.0	37.5	41.0	33.0	41.0
50-51	37.72984642560526	40.0	37.0	41.0	33.0	41.0
52-53	38.11670125455433	40.0	38.0	41.0	33.0	41.0
54-55	38.04843417206787	40.0	38.0	41.0	33.0	41.0
56-57	37.650138821069504	39.5	37.5	41.0	32.0	41.0
58-59	37.42162965467064	39.0	36.5	41.0	32.0	41.0
60-61	36.65282412102596	39.0	35.5	40.0	30.5	41.0
62-63	36.84783259671045	39.0	35.5	40.0	31.0	41.0
64-65	36.59473951094918	38.5	35.5	39.5	31.0	40.5
66-67	36.017625215456974	37.0	35.0	39.0	30.5	40.0
68-69	35.2841550608566	36.5	34.5	39.0	29.5	39.0
70-71	34.358326168823666	35.5	33.5	37.5	29.0	39.0
72-73	33.78117500740797	35.0	33.5	37.0	27.5	37.5
74-75	33.68684815134783	35.0	33.5	37.0	29.0	37.0
76-77	33.524840589660016	35.0	34.0	36.0	29.0	36.5
78-79	33.18854763564225	35.0	33.5	35.5	29.0	36.0
80-81	32.70849879524944	34.5	32.0	35.0	26.5	36.0
82-83	32.32313616672301	34.5	32.0	35.0	26.0	35.5
84-85	32.22282059789572	34.0	32.0	35.0	26.0	35.0
86-87	32.59678200483848	34.5	33.0	35.0	27.0	35.5
88-89	32.44755587621953	35.0	33.0	35.0	26.5	35.0
90-91	32.16635687118212	34.0	32.0	35.0	25.0	35.0
92-93	31.97710182715864	34.0	32.0	35.0	25.0	35.0
94-95	31.749846971638277	34.0	31.5	35.0	25.0	35.0
96-97	31.641398988760763	34.0	31.0	35.0	25.0	35.0
98-99	31.927102244508717	34.0	32.0	35.0	25.0	35.0
100	30.815830366669868	34.0	30.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3304618341785712
1101	2	0.34947458303820156
1101	3	0.3433753640514894
1101	4	0.255859355448095
1101	5	0.20646237808089296
1101	6	0.23543556519479125
1101	7	0.20520375040777594
1101	8	0.21838936913924556
1101	9	0.27837907214960467
1101	10-11	0.3049072774260395
1101	12-13	0.2985376747071484
1101	14-15	0.43664565563866375
1101	16-17	0.45307607275981354
1101	18-19	0.4454780660561397
1101	20-21	0.375304831901758
1101	22-23	0.38113491023455026
1101	24-25	0.3803288195158103
1101	26-27	0.3959960746022091
1101	28-29	0.4046150023318589
1101	30-31	0.4679757880385935
1101	32-33	0.44867212142031576
1101	34-35	0.49480781744787095
1101	36-37	0.5217331299479966
1101	38-39	0.49194804538038284
1101	40-41	0.47120004634915347
1101	42-43	0.5214800158972892
1101	44-45	0.49824523070717674
1101	46-47	0.5229259803218227
1101	48-49	0.6136563573219576
1101	50-51	0.6179543208683853
1101	52-53	0.5774804133547491
1101	54-55	0.6110890383791769
1101	56-57	0.6732165195288715
1101	58-59	0.6750057732353127
1101	60-61	0.6170342290525568
1101	62-63	0.5976671742135196
1101	64-65	0.5456902723620516
1101	66-67	0.47193524254976893
1101	68-69	0.47502537392061583
1101	70-71	0.486412209102312
1101	72-73	0.4881821057976268
1101	74-75	0.44253768466949595
1101	76-77	0.4362470083430168
1101	78-79	0.4628699800608942
1101	80-81	0.5227783041846408
1101	82-83	0.5006570227696088
1101	84-85	0.48824857635222685
1101	86-87	0.5038357788545014
1101	88-89	0.6023582903405753
1101	90-91	0.6114128954970397
1101	92-93	0.5864633553086449
1101	94-95	0.6186084747898803
1101	96-97	0.5938418552349383
1101	98-99	0.6154179201029422
1101	100	0.6737851888220092
1102	1	0.2878265776385902
1102	2	0.26098543869126445
1102	3	0.27870969784125066
1102	4	0.17653836049330351
1102	5	0.19002865481485287
1102	6	0.22576959674508146
1102	7	0.21001067195429357
1102	8	0.20375178053708964
1102	9	0.25297124598722576
1102	10-11	0.26058637883745206
1102	12-13	0.28547392145370054
1102	14-15	0.4161126453949322
1102	16-17	0.46627725736330206
1102	18-19	0.3901575126337775
1102	20-21	0.3394385347556863
1102	22-23	0.40829003091570115
1102	24-25	0.42122236639666255
1102	26-27	0.39585010170213764
1102	28-29	0.35744286863513963
1102	30-31	0.39648465679570677
1102	32-33	0.40969823315781184
1102	34-35	0.377301823031857
1102	36-37	0.45776272400973284
1102	38-39	0.4794624893605146
1102	40-41	0.4626314480657001
1102	42-43	0.4773722180315332
1102	44-45	0.4724442742287849
1102	46-47	0.5764742716175917
1102	48-49	0.6167015294188616
1102	50-51	0.5960048369094082
1102	52-53	0.5788906451680376
1102	54-55	0.5773261526843925
1102	56-57	0.5760041529413158
1102	58-59	0.6195174550095786
1102	60-61	0.5606468579313955
1102	62-63	0.592797614633362
1102	64-65	0.5130024173949437
1102	66-67	0.4738584053937629
1102	68-69	0.4426514756710702
1102	70-71	0.47534301729063344
1102	72-73	0.5210364114835002
1102	74-75	0.4273292358062264
1102	76-77	0.4497006067009366
1102	78-79	0.4459551923162266
1102	80-81	0.47954612470521596
1102	82-83	0.4526869111862979
1102	84-85	0.4998778967339419
1102	86-87	0.5026290111434903
1102	88-89	0.5192278735742519
1102	90-91	0.49258942174061815
1102	92-93	0.5223440197799221
1102	94-95	0.5630389023316908
1102	96-97	0.5632444898870084
1102	98-99	0.5459404045288494
1102	100	0.6034768432457867
1103	1	0.27111009763088845
1103	2	0.28491878484256716
1103	3	0.23533552161380555
1103	4	0.2396748273295799
1103	5	0.18075138581663452
1103	6	0.2032047068571643
1103	7	0.21237667257645398
1103	8	0.21291242094991958
1103	9	0.2763868165794108
1103	10-11	0.2655633214033841
1103	12-13	0.24554997649259747
1103	14-15	0.41681417352216954
1103	16-17	0.4490798640946849
1103	18-19	0.382573540584616
1103	20-21	0.3518965809357084
1103	22-23	0.39172693533008385
1103	24-25	0.3913459660817864
1103	26-27	0.41996062642324716
1103	28-29	0.41647186406644465
1103	30-31	0.44376040786569604
1103	32-33	0.4368607964784559
1103	34-35	0.47030058403279185
1103	36-37	0.4746851005798547
1103	38-39	0.47423089086009185
1103	40-41	0.4977298653449935
1103	42-43	0.48091767911953553
1103	44-45	0.4897051185579926
1103	46-47	0.4879509662346564
1103	48-49	0.560750708888051
1103	50-51	0.575789339647919
1103	52-53	0.505576564334369
1103	54-55	0.5249120367822968
1103	56-57	0.5310961058776584
1103	58-59	0.5097571807817616
1103	60-61	0.4988975845844479
1103	62-63	0.5116489247380684
1103	64-65	0.4902254561769581
1103	66-67	0.44175562872850804
1103	68-69	0.39074337133112635
1103	70-71	0.458396553803631
1103	72-73	0.42906774544836424
1103	74-75	0.40076014217907385
1103	76-77	0.3543880644490258
1103	78-79	0.42832777438899683
1103	80-81	0.4749146231273471
1103	82-83	0.4477881379848796
1103	84-85	0.4752119356941762
1103	86-87	0.4774753233060949
1103	88-89	0.5199108773985657
1103	90-91	0.5078574662747499
1103	92-93	0.5039435848207674
1103	94-95	0.5559473413866272
1103	96-97	0.5881688063987802
1103	98-99	0.5873375106494692
1103	100	0.6611896284002832
1104	1	0.2592135434683769
1104	2	0.2337361955124706
1104	3	0.2534590187056338
1104	4	0.17270294109741968
1104	5	0.14992510401093284
1104	6	0.17684607371171523
1104	7	0.16160723350827055
1104	8	0.16069024864265202
1104	9	0.19166095992434862
1104	10-11	0.20841048721130306
1104	12-13	0.19080738480818127
1104	14-15	0.37943707350631684
1104	16-17	0.4233821361819565
1104	18-19	0.35591983724833653
1104	20-21	0.31210721061705726
1104	22-23	0.3370338426450843
1104	24-25	0.34454208839672873
1104	26-27	0.35963205705663626
1104	28-29	0.37304223750104626
1104	30-31	0.37485361510552906
1104	32-33	0.3807806919149357
1104	34-35	0.41742234200431483
1104	36-37	0.45822413553238306
1104	38-39	0.46947805101098083
1104	40-41	0.43967637814049
1104	42-43	0.3819269855553671
1104	44-45	0.44081448368549303
1104	46-47	0.5203004341620101
1104	48-49	0.6319534597013501
1104	50-51	0.5658425885085094
1104	52-53	0.5494791871158071
1104	54-55	0.549176165522951
1104	56-57	0.522009191874055
1104	58-59	0.5062945905864709
1104	60-61	0.553089889860992
1104	62-63	0.5126499016153829
1104	64-65	0.510763641327344
1104	66-67	0.49912257728990284
1104	68-69	0.4807920342550034
1104	70-71	0.4850987942364995
1104	72-73	0.4369751846072276
1104	74-75	0.4106965463637806
1104	76-77	0.4133609227749844
1104	78-79	0.47094214931438927
1104	80-81	0.47660715614089355
1104	82-83	0.49118768558897585
1104	84-85	0.526349159607264
1104	86-87	0.5469540582863743
1104	88-89	0.6214205084895923
1104	90-91	0.6153637295620058
1104	92-93	0.6100356169793102
1104	94-95	0.6062189047522111
1104	96-97	0.5696113351487533
1104	98-99	0.577127796075672
1104	100	0.6775901235566621
1105	1	0.1758306955404123
1105	2	0.17171005384374638
1105	3	0.207577913576813
1105	4	0.16399860641377018
1105	5	0.1214156636102004
1105	6	0.14714323767616833
1105	7	0.15856362183001949
1105	8	0.11471940925328994
1105	9	0.19739319340023087
1105	10-11	0.1698010722124721
1105	12-13	0.17923654344523499
1105	14-15	0.2692796104106847
1105	16-17	0.3467616990529123
1105	18-19	0.29289294689220924
1105	20-21	0.26436758926443815
1105	22-23	0.261577810204507
1105	24-25	0.2867228808084974
1105	26-27	0.2791623257373743
1105	28-29	0.26390165393231513
1105	30-31	0.331512771024137
1105	32-33	0.274429907989564
1105	34-35	0.29284476786612146
1105	36-37	0.25780759858090363
1105	38-39	0.3236503280584344
1105	40-41	0.3180679925868475
1105	42-43	0.27701569005238724
1105	44-45	0.3327407478435447
1105	46-47	0.33788529335975426
1105	48-49	0.44508296034085504
1105	50-51	0.3952351744659097
1105	52-53	0.35616582992123824
1105	54-55	0.3610021638205865
1105	56-57	0.33441203281172704
1105	58-59	0.39024331504849386
1105	60-61	0.38480958218612216
1105	62-63	0.3797431880029549
1105	64-65	0.35608130506610536
1105	66-67	0.3501569245980889
1105	68-69	0.26566351913259467
1105	70-71	0.32095620207186926
1105	72-73	0.3310552460938183
1105	74-75	0.31128378213473695
1105	76-77	0.25921401578984415
1105	78-79	0.2834591396164683
1105	80-81	0.3917571896599341
1105	82-83	0.3444012957838609
1105	84-85	0.29194787238696307
1105	86-87	0.3157696917835153
1105	88-89	0.3950155656884249
1105	90-91	0.3547611603418446
1105	92-93	0.40216240649183277
1105	94-95	0.37617230755558495
1105	96-97	0.31217425417655065
1105	98-99	0.349043813529768
1105	100	0.42076688190570266
1106	1	0.1334218001898293
1106	2	0.15021715411430137
1106	3	0.18816231584973764
1106	4	0.13767085423833691
1106	5	0.11051775729283264
1106	6	0.11048009599520725
1106	7	0.11278539229570583
1106	8	0.1154387887871593
1106	9	0.17085273578563687
1106	10-11	0.16971590324227748
1106	12-13	0.1375155609341121
1106	14-15	0.18282709624902083
1106	16-17	0.2890693243126776
1106	18-19	0.21387806920666463
1106	20-21	0.2011153188335797
1106	22-23	0.22940667522554747
1106	24-25	0.23776761845990535
1106	26-27	0.2761849056282415
1106	28-29	0.21172127607160007
1106	30-31	0.2404214864967429
1106	32-33	0.22911945683233625
1106	34-35	0.22586482689080611
1106	36-37	0.2526835291120406
1106	38-39	0.271171891901659
1106	40-41	0.22964529411110846
1106	42-43	0.23565452970663614
1106	44-45	0.26069578710077934
1106	46-47	0.25956711094310236
1106	48-49	0.3509147729923683
1106	50-51	0.31662970215914044
1106	52-53	0.3168743218905803
1106	54-55	0.32005912508381584
1106	56-57	0.27758849337056546
1106	58-59	0.2840853241447121
1106	60-61	0.3085178901951906
1106	62-63	0.3305821931993691
1106	64-65	0.32110204344186855
1106	66-67	0.27439031743961806
1106	68-69	0.2097678941137957
1106	70-71	0.22087898436316067
1106	72-73	0.23538352006618624
1106	74-75	0.237706515475395
1106	76-77	0.18729068507481372
1106	78-79	0.24483016805881874
1106	80-81	0.23031248884471722
1106	82-83	0.2707739457392435
1106	84-85	0.26292372313333345
1106	86-87	0.25330156578699103
1106	88-89	0.2773080401540966
1106	90-91	0.21011909325511624
1106	92-93	0.25602411206169506
1106	94-95	0.2675906382805451
1106	96-97	0.25594348458932714
1106	98-99	0.23279916564450787
1106	100	0.29738726771915225
1107	1	0.0559493138995677
1107	2	0.06248584295505566
1107	3	0.05607094485330322
1107	4	0.042617575112053885
1107	5	0.06982622594091481
1107	6	0.03416524095541007
1107	7	0.07866055174222453
1107	8	0.09711783680335628
1107	9	0.08740706433760437
1107	10-11	0.07065302611694335
1107	12-13	0.07904337273872386
1107	14-15	0.1728731200310918
1107	16-17	0.2610415655952494
1107	18-19	0.1884619413310702
1107	20-21	0.14542972863170434
1107	22-23	0.1375644085879486
1107	24-25	0.11984200707306059
1107	26-27	0.17967055394019127
1107	28-29	0.16747801817931673
1107	30-31	0.19321963047966761
1107	32-33	0.15570629696374283
1107	34-35	0.10630131868240511
1107	36-37	0.1184727189610939
1107	38-39	0.14619608871472423
1107	40-41	0.11364195648157249
1107	42-43	0.12622847419528682
1107	44-45	0.15412174911833887
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2216	74-75	-1.3660715699633954
2216	76-77	-1.3809489770376615
2216	78-79	-1.4206288200491883
2216	80-81	-1.533532965527833
2216	82-83	-1.5487952123589963
2216	84-85	-1.5301663678822486
2216	86-87	-1.4987006419266677
2216	88-89	-1.4704306322377576
2216	90-91	-1.6666489145514305
2216	92-93	-1.7804345704179845
2216	94-95	-1.7748625938061018
2216	96-97	-1.6776198930100037
2216	98-99	-1.761804812205952
2216	100	-2.0027083568022768
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	15.0
8	91.0
9	132.0
10	178.0
11	150.0
12	126.0
13	217.0
14	335.0
15	541.0
16	855.0
17	1748.0
18	3003.0
19	4334.0
20	6636.0
21	10251.0
22	14910.0
23	19986.0
24	25163.0
25	31037.0
26	38754.0
27	48261.0
28	60053.0
29	74386.0
30	92688.0
31	115197.0
32	145798.0
33	192467.0
34	272376.0
35	438488.0
36	913470.0
37	1719273.0
38	1417082.0
39	102402.0
40	165.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	55.07380709085079	15.771713808927245	12.791832075564882	16.362647024657086
2	12.305188385498447	31.557851217392713	26.2556642076335	29.881296189475343
3	41.19610243244094	18.53753051224046	28.385143640768266	11.881223414550337
4	10.069919338829287	12.73576920406923	48.64075114181984	28.55356031528164
5	9.434427854467295	45.88097300042084	33.52077920722864	11.16381993788323
6	24.803104418307637	38.63584508237546	12.843723827252212	23.717326672064686
7	24.419470288755875	33.68078312973312	22.597928169517893	19.301818411993114
8	21.744382873393757	15.236922180150797	19.178153417993236	43.840541528462204
9	14.219348147643206	30.611473722760508	31.968957490445288	23.200220639151
10-11	29.23515743025139	17.988483735286763	25.799702203045356	26.97665663141649
12-13	40.81952426264675	19.225631276771267	20.499392407845626	19.455452052736355
14-15	25.147470253582842	24.569779342609117	36.434027847517285	13.848722556290758
16-17	18.200567371668612	27.165916173431366	32.131474694209764	22.502041760690258
18-19	15.706128497723961	41.52146199301251	22.551756254737583	20.22065325452595
20-21	24.35044073152617	24.44400564606569	18.019658283299016	33.18589533910913
22-23	18.45191640199716	26.857520857070117	24.040590425154523	30.649972315778196
24-25	31.971746095342233	25.300535529707673	20.125724971863647	22.601993403086443
26-27	26.905231486807057	16.34802348190944	37.24106953512048	19.505675496163022
28-29	17.220842503082086	40.17620698760722	23.82873930768386	18.77421120162683
30-31	22.497816079031725	22.921331980071823	22.44615146912431	32.13470047177214
32-33	25.26578977090976	25.56373515892087	26.968078969135217	22.202396101034154
34-35	32.98017331018311	20.896255866714814	25.266380740716137	20.85719008238594
36-37	25.922613530563776	17.93256524662094	36.50162436184542	19.64319686096986
38-39	21.07479798574825	29.20272896941104	30.965864406234388	18.756608638606316
40-41	20.94658695580158	32.31142814836224	24.555521674641145	22.186463221195044
42-43	17.99604839035031	31.708719903842542	16.796045190666383	33.499186515140764
44-45	24.808980866393945	20.11214084111978	21.184910312281296	33.89396798020498
46-47	32.765198150860925	28.941662806178453	23.666366522402658	14.626772520557967
48-49	27.690998789683093	25.55651101805737	25.978782935922762	20.773707256336774
50-51	11.910345204160702	28.815744809903997	39.08354792083147	20.190362065103827
52-53	23.200697881193605	34.4570753157655	19.61117103775657	22.73105576528432
54-55	17.455266871957246	23.029066099439564	26.87911656419527	32.63655046440792
56-57	28.45548326109684	18.06772249500744	25.93401403273005	27.542780211165667
58-59	39.21969035105048	19.871169732364702	20.883279610580765	20.025860306004056
60-61	24.671580128953387	24.825861627476783	36.41169440542629	14.090863838143544
62-63	18.211047794467678	26.676785118224768	32.11096920199205	23.001197885315502
64-65	15.793184999695681	40.60062081018337	22.635921781394824	20.97027240872612
66-67	24.001730986236105	24.392785366227756	18.30022333791672	33.30526030961942
68-69	18.344464277811124	26.66693099562196	24.178665157904813	30.8099395686621
70-71	31.116460927141258	25.202720145813647	20.36676527799552	23.314053649049576
72-73	26.22018733760819	16.689760844829557	36.68076315824712	20.409288659315138
74-75	17.187862474910432	39.0538794010432	23.899919622954794	19.858338501091577
76-77	22.14972851451857	22.879785124534642	22.69074306026243	32.27974330068435
78-79	24.706318224142677	25.273592164277638	26.910828122395603	23.109261489184085
80-81	31.812922995164428	20.87660414060738	25.30158634653037	22.008886517697825
82-83	25.01747013643531	18.335230985331346	35.78206521053938	20.865233667693964
84-85	20.56460882736026	28.663992710237146	30.528916465091044	20.24248199731155
86-87	20.45951116998453	31.629800015389787	24.779615229279546	23.131073585346144
88-89	17.780365348257774	31.0196749260247	17.52490362691129	33.67505609880624
90-91	23.932079033611647	20.259501412556606	21.66177207600087	34.146647477830875
92-93	31.007864970415067	28.272854027136823	23.983168949400078	16.736112053048032
94-95	26.216419815517227	25.199676066556254	26.243060680656715	22.340843437269804
96-97	12.025082466010648	28.036574234364668	38.092280200627734	21.84606309899695
98-99	22.422534079718485	32.99598943476993	20.46341217839442	24.118064307117166
100	20.806144900518834	14.455193839784913	21.66534433499363	43.073316924702624
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	105.0
1	61.5
2	75.0
3	144.5
4	106.5
5	62.5
6	118.5
7	261.0
8	437.5
9	536.5
10	462.0
11	356.5
12	378.0
13	446.5
14	542.5
15	609.5
16	671.5
17	788.5
18	962.0
19	1128.0
20	1257.5
21	1480.0
22	1796.0
23	2123.0
24	2671.0
25	3520.5
26	4584.0
27	5705.0
28	6909.5
29	8610.0
30	10608.0
31	12568.0
32	14870.0
33	16933.0
34	18748.0
35	20981.0
36	23460.0
37	26315.0
38	28123.5
39	28981.5
40	29575.0
41	29908.5
42	31002.5
43	33037.5
44	36019.0
45	44882.5
46	89599.5
47	579632.5
48	1659952.5
49	1685375.0
50	700085.5
51	211347.5
52	78214.5
53	45873.5
54	38312.0
55	33582.5
56	30350.0
57	27369.0
58	24023.5
59	21513.5
60	17873.0
61	13693.0
62	10571.5
63	8317.0
64	6148.5
65	4138.5
66	3011.5
67	2265.5
68	1727.5
69	1290.5
70	919.0
71	659.5
72	529.5
73	450.0
74	317.0
75	207.5
76	144.5
77	88.0
78	47.5
79	19.0
80	13.5
81	11.5
82	6.0
83	4.5
84	4.5
85	3.0
86	1.5
87	1.0
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.002991008888165482
2	0.0035648652446158362
3	1.2172710591371148E-4
4	0.05121233241655433
5	0.0018780753483829773
6	0.005425551006439712
7	1.738958655910164E-5
8	5.216875967730492E-4
9	3.477917311820328E-5
10-11	9.564272607505902E-5
12-13	0.0
14-15	2.608437983865246E-5
16-17	2.8692817822517706E-4
18-19	1.2172710591371148E-4
20-21	2.608437983865246E-4
22-23	0.0
24-25	0.0
26-27	2.3475941854787216E-4
28-29	2.608437983865246E-5
30-31	2.8692817822517706E-4
32-33	3.043177647842787E-4
34-35	1.9998024542966888E-4
36-37	3.477917311820328E-5
38-39	7.912261884391246E-4
40-41	5.999407362890065E-4
42-43	0.0
44-45	5.477719766117017E-4
46-47	0.0
48-49	1.3911669247281311E-4
50-51	0.0
52-53	3.3040214462293113E-4
54-55	3.477917311820328E-4
56-57	2.0867503870921968E-4
58-59	9.738168473096919E-4
60-61	7.042782556436164E-4
62-63	3.043177647842787E-4
64-65	3.130125580638295E-4
66-67	0.0015476732037600462
68-69	0.0027388598830585084
70-71	0.0037300663169273014
72-73	0.00349530689837943
74-75	0.002956229715047279
76-77	0.004529987298645978
78-79	0.0014520304776849868
80-81	0.0010259856069869968
82-83	7.042782556436164E-4
84-85	6.608042892458623E-4
86-87	8.69479327955082E-6
88-89	0.0
90-91	3.477917311820328E-5
92-93	1.0433751935460984E-4
94-95	6.955834623640656E-5
96-97	2.956229715047279E-4
98-99	1.2172710591371148E-4
100	0.07660112879284273
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5750568.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.500102890068156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.49127146842223	37.58334639300681
2	14.310728665373922	13.881436254049662
3	4.476969597954653	6.514004584095232
4	1.845262728229327	3.5798172871332095
5	0.8259949777697817	2.003042070425699
6	0.4106437572924154	1.1949758687907792
7	0.23263122586542348	0.7897846872941006
8	0.13457443886647713	0.5221499305117863
9	0.08003873203454333	0.34936980649793514
>10	0.18809848215077943	1.233416548522089
>50	0.0014414522722379283	0.04923769284681215
>100	9.758042598124137E-4	0.10184889879667014
>500	1.5914785429997405E-4	0.05066544790313326
>1k	4.7744275818674924E-4	0.5266297100530751
>5k	9.548855163723401E-5	0.3083456008850958
>10k+	6.365903442482267E-4	31.311929219187956
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	726550	12.634404114515297	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	176289	3.065592824917469	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	170912	2.9720890179891795	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	142954	2.485910956969816	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	123470	2.1470922524522797	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	122247	2.1258247880904984	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	121772	2.117564734474925	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	97441	1.6944587039054229	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	63587	1.1057516405335959	No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT	59431	1.0334805187939695	No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA	33794	0.5876636881782809	No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG	33131	0.5761343922895964	No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	28278	0.49174272871827623	No Hit
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	22007	0.3826926314061498	No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA	21506	0.37398044854003987	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC	19065	0.3315324677492728	No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA	18154	0.3156905543939312	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT	17792	0.30939552405953635	No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG	17555	0.3052741920450293	No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC	12388	0.21542219829415113	No Hit
GAGTAGGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	8374	0.14562039784591715	No Hit
AGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG	6125	0.10651121767449755	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.738958655910164E-5	0.0
8	0.0	0.0	0.0	3.477917311820328E-5	0.0
9	0.0	0.0	0.0	1.0433751935460984E-4	0.0
10-11	0.0	0.0	0.0	5.129928034934984E-4	0.0
12-13	0.0	0.0	0.0	8.433949481164295E-4	0.0
14-15	0.0	0.0	0.0	0.001538978410480495	0.0
16-17	0.0	0.0	0.0	0.0030344828545632364	0.0
18-19	0.0	0.0	0.0	0.00488647382310756	0.0
20-21	0.0	0.0	0.0	0.008964331871216896	0.0
22-23	0.0	0.0	0.0	0.014555083949968073	0.0
24-25	0.0	0.0	0.0	0.018945954556141238	0.0
26-27	0.0	0.0	0.0	0.022728189632745845	0.0
28-29	0.0	0.0	0.0	0.03302282487573402	0.0
30-31	0.0	0.0	0.0	0.044560815557697954	0.0
32-33	0.0	0.0	0.0	0.08892165086996624	0.0
34-35	0.0	0.0	0.0	0.13502144483814468	0.0
36-37	0.0	0.0	0.0	0.17448711153402585	0.0
38-39	0.0	0.0	0.0	0.23669835744921197	0.0
40-41	0.0	0.0	0.0	0.29965735558643947	0.0
42-43	0.0	0.0	0.0	0.3809188935771214	0.0
44-45	0.0	0.0	0.0	0.48604763912017035	0.0
46-47	0.0	0.0	0.0	0.6272249976002371	0.0
48-49	0.0	0.0	0.0	0.7160683953306873	0.0
50-51	0.0	0.0	0.0	0.828074722357861	0.0
52-53	0.0	0.0	0.0	0.9426373186092226	0.0
54-55	0.0	0.0	0.0	1.108733954628482	0.0
56-57	0.0	0.0	0.0	1.4033918040791797	0.0
58-59	0.0	0.0	0.0	1.5909037159459727	0.0
60-61	0.0	0.0	0.0	1.7779982777353474	0.0
62-63	0.0	0.0	0.0	1.9647363530002602	0.0
64-65	0.0	0.0	0.0	2.1487964319350716	0.0
66-67	0.0	0.0	0.0	2.417517713032869	0.0
68-69	0.0	0.0	0.0	2.7436246297757023	0.0
70-71	0.0	0.0	0.0	3.0271183646554567	0.0
72-73	0.0	0.0	0.0	3.242071044112512	0.0
74-75	0.0	0.0	0.0	3.5569268983516062	0.0
76-77	0.0	0.0	0.0	3.809162851391376	0.0
78-79	0.0	0.0	0.0	4.412503251852687	0.0
80-81	0.0	0.0	0.0	4.965944233682656	0.0
82-83	8.69479327955082E-6	0.0	0.0	5.285538750259105	0.0
84-85	1.738958655910164E-5	0.0	0.0	5.654137121759103	0.0
86-87	1.738958655910164E-5	0.0	0.0	6.0032678511061865	0.0
88	1.738958655910164E-5	0.0	0.0	6.3488858839683315	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACGCA	9550	0.0	32.84849	3
TACGCAG	9685	0.0	32.438614	4
GAGTACG	9705	0.0	32.275684	1
AGTACGC	9840	0.0	31.880674	2
CGCAGTG	10040	0.0	31.225769	6
GCTTATG	1730	0.0	29.904968	1
GTACTAG	380	0.0	29.704647	1
ACGCAGT	10750	0.0	29.2079	5
TATGTAC	1795	0.0	26.463202	4
AGTAAGG	845	0.0	25.604305	94
TTAGGTA	435	0.0	24.867468	3
GTACCGT	115	1.3776463E-4	24.523046	6
TTATGTA	2020	0.0	23.748808	3
CTTAGGT	455	0.0	22.74092	2
TTCGTAT	105	0.0023272997	22.39636	1
CCCGTAT	150	3.2768767E-5	21.948435	1
CTTATGT	2420	0.0	21.378345	2
GCTTAGG	510	0.0	21.210672	1
GTGATCG	290	1.2187229E-10	21.067707	8
GTAGGCA	4385	0.0	21.022263	3
>>END_MODULE