FastQCFastQC Report
Mon 16 Jan 2017
ERR1146633_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146633_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4608069
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36650.038.3650131
GTATCAA58800.033.7499581
CAACGCA57600.031.5026555
ATCAACG62100.029.4488723
AACGCAG68550.027.6272966
TATCAAC72800.026.5412332
TCAACGC72300.025.3587324
GTACATG124950.025.3289341
ACGCAGA73400.024.7117637
TACATGG125550.024.226922
CGCAGAG74550.024.1412038
ACATGGG123550.023.6678223
CGTTAAC4600.021.4683951
CATGGGG96000.021.3019454
TGATCGC13200.021.0023069
GTGATCG12700.020.7201128
TATTCGC3700.020.3192259
GTACTAG21500.019.9040321
GTATAAG27450.019.7012421
ATGGGGG67000.018.4528435
Produced by FastQC (version 0.11.5)