##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146633_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4608069
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.433639557046565	31.0	31.0	34.0	28.0	34.0
2	31.528836482266215	33.0	31.0	34.0	28.0	34.0
3	31.69198898714407	33.0	31.0	34.0	29.0	34.0
4	35.19266920699321	37.0	35.0	37.0	32.0	37.0
5	35.172781266947176	37.0	35.0	37.0	32.0	37.0
6	35.25479479582445	37.0	35.0	37.0	32.0	37.0
7	35.12854104398176	37.0	35.0	37.0	32.0	37.0
8	35.12498055042145	37.0	35.0	37.0	32.0	37.0
9	36.76686330000701	39.0	37.0	39.0	33.0	39.0
10-11	36.766002201789945	39.0	37.0	39.0	33.0	39.0
12-13	36.733369443903726	39.0	37.0	39.0	33.0	39.0
14-15	38.09282022469716	40.0	38.0	41.0	33.0	41.0
16-17	38.109246519529115	40.0	38.0	41.0	33.0	41.0
18-19	38.12228658034417	40.0	38.0	41.0	33.0	41.0
20-21	38.216463338548095	40.0	38.0	41.0	33.0	41.0
22-23	38.217777120958914	40.0	38.0	41.0	33.0	41.0
24-25	38.229614725821165	40.0	38.0	41.0	33.0	41.0
26-27	38.28319953542362	40.0	38.0	41.0	33.0	41.0
28-29	38.25974252989701	40.0	38.0	41.0	33.0	41.0
30-31	38.222504914748455	40.0	38.0	41.0	33.0	41.0
32-33	38.19116174258676	40.0	38.0	41.0	33.0	41.0
34-35	38.14710001087224	40.0	38.0	41.0	33.0	41.0
36-37	38.085980049343874	40.0	38.0	41.0	32.5	41.0
38-39	38.03333847648548	40.0	38.0	41.0	32.0	41.0
40-41	37.987723599624914	40.0	38.0	41.0	32.0	41.0
42-43	37.84605471402446	40.0	38.0	41.0	32.0	41.0
44-45	37.73938378526884	40.0	37.5	41.0	31.5	41.0
46-47	37.64341267459319	40.0	37.0	41.0	31.0	41.0
48-49	37.54588852727683	40.0	37.0	41.0	31.0	41.0
50-51	37.42343864642652	40.0	37.0	41.0	31.0	41.0
52-53	37.306479134752536	40.0	36.0	41.0	31.0	41.0
54-55	37.19704772215867	40.0	36.0	41.0	31.0	41.0
56-57	37.024595117824845	39.0	35.5	41.0	30.0	41.0
58-59	36.838501984236785	39.0	35.0	41.0	30.0	41.0
60-61	36.6296268567159	39.0	35.0	41.0	30.0	41.0
62-63	36.394313648515244	38.5	35.0	40.5	30.0	41.0
64-65	36.108731987303145	38.0	35.0	40.0	30.0	41.0
66-67	35.876717991852985	37.0	35.0	40.0	29.5	41.0
68-69	35.61481935274841	37.0	35.0	40.0	29.0	41.0
70-71	35.32346976575221	36.0	34.0	39.0	29.0	41.0
72-73	34.977789612091314	36.0	34.0	39.0	29.0	41.0
74-75	34.63678169749629	35.5	34.0	38.5	29.0	40.0
76-77	34.34877928260189	35.0	34.0	37.0	29.0	39.5
78-79	34.03010317336828	35.0	34.0	37.0	28.0	39.0
80-81	33.77979149183747	35.0	34.0	36.5	28.0	39.0
82-83	33.503558214948605	35.0	34.0	36.0	28.0	37.5
84-85	33.29937388090326	35.0	34.0	36.0	28.0	37.0
86-87	33.093301771305946	35.0	33.5	35.0	27.5	37.0
88-89	32.922896336838704	35.0	33.0	35.0	27.0	36.0
90-91	32.79141360513482	35.0	33.0	35.0	27.0	36.0
92-93	32.65933539623647	35.0	33.0	35.0	27.0	36.0
94-95	32.553394382766406	35.0	33.0	35.0	27.0	36.0
96-97	32.48086617192581	35.0	33.0	35.0	26.0	35.0
98-99	32.39938236601925	35.0	33.0	35.0	26.0	35.0
100	31.281654419671234	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2554105160750453
1101	2	0.27747324595481615
1101	3	0.3275337387834689
1101	4	0.3034397032833809
1101	5	0.2414653440640393
1101	6	0.28491393833690637
1101	7	0.2542101310057632
1101	8	0.28740331363573546
1101	9	0.467239459495957
1101	10-11	0.46499405995474774
1101	12-13	0.4752329948974534
1101	14-15	0.5250020908702382
1101	16-17	0.4967915331297448
1101	18-19	0.4655458314146088
1101	20-21	0.48677708450593826
1101	22-23	0.5156482925459969
1101	24-25	0.5634909365068168
1101	26-27	0.5388850112188663
1101	28-29	0.5652337801005061
1101	30-31	0.542085213865569
1101	32-33	0.5795319020018042
1101	34-35	0.5418062538860013
1101	36-37	0.516736850290485
1101	38-39	0.5765245068286973
1101	40-41	0.5210894854147909
1101	42-43	0.5144578325292883
1101	44-45	0.44775480867193806
1101	46-47	0.47232870900139545
1101	48-49	0.4730004687646172
1101	50-51	0.46434605389465844
1101	52-53	0.39668619708771047
1101	54-55	0.3930983804179178
1101	56-57	0.43557600584868794
1101	58-59	0.4115835766362892
1101	60-61	0.46174565670409606
1101	62-63	0.45522129955306667
1101	64-65	0.4041962161429069
1101	66-67	0.3704436133080762
1101	68-69	0.3697225483727422
1101	70-71	0.41298245100325914
1101	72-73	0.4290705816300431
1101	74-75	0.36725059813309713
1101	76-77	0.36566421810654504
1101	78-79	0.3159932262945375
1101	80-81	0.34434616694998965
1101	82-83	0.2816682175233396
1101	84-85	0.26630389603776194
1101	86-87	0.2985569235720078
1101	88-89	0.2801988056655347
1101	90-91	0.3287273709061438
1101	92-93	0.29663795466448306
1101	94-95	0.30477320189216073
1101	96-97	0.29954612941713066
1101	98-99	0.3330809028545829
1101	100	0.3276784168832627
1102	1	0.32361003634320795
1102	2	0.3947961475857902
1102	3	0.361389970836246
1102	4	0.35000847080837616
1102	5	0.309652154810621
1102	6	0.35634281440405857
1102	7	0.3255203848714885
1102	8	0.3755265017114624
1102	9	0.4340794078367267
1102	10-11	0.4613364188681075
1102	12-13	0.4534006526509131
1102	14-15	0.5568031762235748
1102	16-17	0.45779267892012143
1102	18-19	0.4616198829445253
1102	20-21	0.46023084610067855
1102	22-23	0.49040648607886084
1102	24-25	0.561018508065267
1102	26-27	0.5162030360329695
1102	28-29	0.5417494559448173
1102	30-31	0.5269418189015767
1102	32-33	0.5655198534890786
1102	34-35	0.468114215772907
1102	36-37	0.5302280785312661
1102	38-39	0.5415501922884616
1102	40-41	0.5836876323494806
1102	42-43	0.5609638059191298
1102	44-45	0.4665691229028326
1102	46-47	0.5500538593135289
1102	48-49	0.5663516488820832
1102	50-51	0.6038414978297126
1102	52-53	0.5625922827648893
1102	54-55	0.5530294982229051
1102	56-57	0.5796895838201195
1102	58-59	0.5019586673160248
1102	60-61	0.4725739416278003
1102	62-63	0.4868712069953389
1102	64-65	0.5516224868434421
1102	66-67	0.5045725968119044
1102	68-69	0.48705646776571143
1102	70-71	0.47177993919235917
1102	72-73	0.49293420738302274
1102	74-75	0.4908467291158942
1102	76-77	0.4938377102980951
1102	78-79	0.43505957858394595
1102	80-81	0.359026360983151
1102	82-83	0.393293486122154
1102	84-85	0.4190906704697994
1102	86-87	0.3919185812257169
1102	88-89	0.36992190073145537
1102	90-91	0.3215374353232292
1102	92-93	0.3637073937469495
1102	94-95	0.36332653647541235
1102	96-97	0.3719879429811286
1102	98-99	0.39175580244794617
1102	100	0.3949784612784448
1103	1	0.2547124666968372
1103	2	0.28097063430588065
1103	3	0.26973342522802923
1103	4	0.2603538580028868
1103	5	0.2320664741944185
1103	6	0.34877601951365733
1103	7	0.2932265909942302
1103	8	0.2955723787466411
1103	9	0.3295117553183573
1103	10-11	0.38320425024008387
1103	12-13	0.3607392090729036
1103	14-15	0.46140754522267713
1103	16-17	0.42324227397404
1103	18-19	0.45942718716524666
1103	20-21	0.47730739515774445
1103	22-23	0.40385876792295505
1103	24-25	0.4610057140863262
1103	26-27	0.46614180185466125
1103	28-29	0.4825153538697222
1103	30-31	0.5203231872643101
1103	32-33	0.46848836239042413
1103	34-35	0.4767178253240445
1103	36-37	0.45883246129085364
1103	38-39	0.4517364055666562
1103	40-41	0.37726014046407386
1103	42-43	0.4764007653489415
1103	44-45	0.39556442369566724
1103	46-47	0.4331582825355156
1103	48-49	0.4624486498489162
1103	50-51	0.3768171741987274
1103	52-53	0.3736079313901399
1103	54-55	0.3306268151895537
1103	56-57	0.4133408008425903
1103	58-59	0.39892416267016984
1103	60-61	0.40396736265039834
1103	62-63	0.43664263543571025
1103	64-65	0.3489646319137236
1103	66-67	0.41911015535814755
1103	68-69	0.40947392694374685
1103	70-71	0.3627939136247136
1103	72-73	0.3553446563561309
1103	74-75	0.37376290119670585
1103	76-77	0.41083899712228344
1103	78-79	0.35126758956961623
1103	80-81	0.274087746417635
1103	82-83	0.2920097034378486
1103	84-85	0.35492971249510674
1103	86-87	0.2682191795558282
1103	88-89	0.23538941718479123
1103	90-91	0.27148335441526683
1103	92-93	0.26125044742454406
1103	94-95	0.2394443441760643
1103	96-97	0.3005005345388909
1103	98-99	0.2327155321569805
1103	100	0.22292980506123072
1104	1	0.22089615980643273
1104	2	0.24472688287465516
1104	3	0.2845705975571384
1104	4	0.2361486501174852
1104	5	0.21806383888042546
1104	6	0.2561980518660931
1104	7	0.31230712401229255
1104	8	0.3332688892434703
1104	9	0.407091039995386
1104	10-11	0.4199262582914045
1104	12-13	0.4731458567327138
1104	14-15	0.5260139850757994
1104	16-17	0.530366017586239
1104	18-19	0.48257806550650173
1104	20-21	0.48339867604617126
1104	22-23	0.4897276509852233
1104	24-25	0.4980956379925132
1104	26-27	0.45358367619598994
1104	28-29	0.47843044461140494
1104	30-31	0.4742027737991563
1104	32-33	0.5022237047833045
1104	34-35	0.4792642130262479
1104	36-37	0.47299902585309184
1104	38-39	0.4226370225807088
1104	40-41	0.375326222450191
1104	42-43	0.46724572407548237
1104	44-45	0.4423462672953278
1104	46-47	0.4308289157696592
1104	48-49	0.4730435596673104
1104	50-51	0.4544824305430737
1104	52-53	0.4500635313382375
1104	54-55	0.3666033432509579
1104	56-57	0.42533875101447904
1104	58-59	0.38309744023906234
1104	60-61	0.42817345953721997
1104	62-63	0.3848292398680897
1104	64-65	0.4140318882707277
1104	66-67	0.3860983816847323
1104	68-69	0.3898221059210698
1104	70-71	0.3520163663074811
1104	72-73	0.3621835482231788
1104	74-75	0.394072295656521
1104	76-77	0.3660092176244518
1104	78-79	0.3263588416439376
1104	80-81	0.2858195583243912
1104	82-83	0.30045173942054504
1104	84-85	0.26130040914642905
1104	86-87	0.24333987326560447
1104	88-89	0.26820142700836413
1104	90-91	0.23553543128209498
1104	92-93	0.20748532766742755
1104	94-95	0.26785960134404263
1104	96-97	0.2609740669008218
1104	98-99	0.29322381924524166
1104	100	0.3762192227700041
1105	1	0.136768609328076
1105	2	0.1836456097351089
1105	3	0.2124624524740426
1105	4	0.23086882608872372
1105	5	0.14607798729770138
1105	6	0.2393035556932901
1105	7	0.11580812263055407
1105	8	0.19925880547989294
1105	9	0.23845420114648874
1105	10-11	0.2981760606097126
1105	12-13	0.27353434391209674
1105	14-15	0.372533439634843
1105	16-17	0.33038359261065153
1105	18-19	0.35420094878557506
1105	20-21	0.3193484407431697
1105	22-23	0.3562818867714199
1105	24-25	0.4058429611050087
1105	26-27	0.37929313715117985
1105	28-29	0.3559728783282168
1105	30-31	0.3840470048795268
1105	32-33	0.4001171059141271
1105	34-35	0.38910818279570947
1105	36-37	0.4018784493146441
1105	38-39	0.399757648245604
1105	40-41	0.3972152658111554
1105	42-43	0.40730847004836335
1105	44-45	0.3188829153648669
1105	46-47	0.3707395041395145
1105	48-49	0.3672998863961183
1105	50-51	0.3414030137561497
1105	52-53	0.31430945863991155
1105	54-55	0.3548386039910767
1105	56-57	0.3365559095541215
1105	58-59	0.3004072490214469
1105	60-61	0.38467915645077255
1105	62-63	0.4281901624060609
1105	64-65	0.42333000197187687
1105	66-67	0.3592320749850231
1105	68-69	0.3141301565290746
1105	70-71	0.3000719407147514
1105	72-73	0.2550460799509793
1105	74-75	0.27663156264809885
1105	76-77	0.2514315934676148
1105	78-79	0.26311201043054666
1105	80-81	0.24518504170811184
1105	82-83	0.3051029614185694
1105	84-85	0.23839826343304793
1105	86-87	0.24299718268765247
1105	88-89	0.23656309801540232
1105	90-91	0.20568405820158375
1105	92-93	0.23073721935300995
1105	94-95	0.2736386038486671
1105	96-97	0.2736276738460788
1105	98-99	0.2985787514921867
1105	100	0.4811412972470457
1106	1	-0.01053505938319077
1106	2	0.032438382914968145
1106	3	0.05110229778725284
1106	4	0.045222448364583556
1106	5	-0.014846836212534242
1106	6	0.09538240927501107
1106	7	0.07053932591064438
1106	8	0.13256342194735993
1106	9	0.1622784090469196
1106	10-11	0.19964531756329507
1106	12-13	0.17246857718920694
1106	14-15	0.17672795467550628
1106	16-17	0.17708178520583573
1106	18-19	0.1977503083361256
1106	20-21	0.2179276983559717
1106	22-23	0.21269944328495427
1106	24-25	0.256662484142673
1106	26-27	0.2543630049347456
1106	28-29	0.1919722830213786
1106	30-31	0.20391358460330622
1106	32-33	0.2141055405557779
1106	34-35	0.23280662999037105
1106	36-37	0.201596610806142
1106	38-39	0.19252218173495095
1106	40-41	0.18903803269706998
1106	42-43	0.249112282521871
1106	44-45	0.21792967008030217
1106	46-47	0.222917515687989
1106	48-49	0.17641201645861315
1106	50-51	0.1578921356598073
1106	52-53	0.15651092118851295
1106	54-55	0.1302177272281142
1106	56-57	0.20246532010617102
1106	58-59	0.1809705161363766
1106	60-61	0.21219240230085745
1106	62-63	0.20120727242522918
1106	64-65	0.19186364208654538
1106	66-67	0.20281398000062012
1106	68-69	0.16035534870685098
1106	70-71	0.10525564100650087
1106	72-73	0.13608910802089724
1106	74-75	0.10315773443250009
1106	76-77	0.14093514964083909
1106	78-79	0.11703993375400046
1106	80-81	0.11691593929329258
1106	82-83	0.06541705694250055
1106	84-85	0.05557253616703406
1106	86-87	0.03883615958726949
1106	88-89	0.013212253466519996
1106	90-91	0.03736034819694822
1106	92-93	0.1060306830209683
1106	94-95	0.09457059684968527
1106	96-97	0.02417635077210889
1106	98-99	0.09020403951777212
1106	100	0.15918516582969033
1107	1	-0.006764299825352538
1107	2	0.06838681004999003
1107	3	0.09722463543046089
1107	4	0.022987472866432768
1107	5	0.01723098704296433
1107	6	0.08096322515201138
1107	7	0.060154755868708776
1107	8	0.02220986310921802
1107	9	0.0930564237974636
1107	10-11	0.08763781633223999
1107	12-13	0.13930367929052068
1107	14-15	0.10577414754030201
1107	16-17	0.1098593714924121
1107	18-19	0.10477504319955244
1107	20-21	0.16191471121876333
1107	22-23	0.1903064616124439
1107	24-25	0.24464255999163242
1107	26-27	0.20074745697046126
1107	28-29	0.15425467304827833
1107	30-31	0.19673083204148156
1107	32-33	0.2230094552621722
1107	34-35	0.18721457125554508
1107	36-37	0.1780311382724875
1107	38-39	0.14324772981562006
1107	40-41	0.14514870915964195
1107	42-43	0.1657757743142696
1107	44-45	0.13758865285980448
1107	46-47	0.13746372935368356
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2216	48-49	-0.44797367842496527
2216	50-51	-0.4873253445760213
2216	52-53	-0.4838964701324855
2216	54-55	-0.4316597990258231
2216	56-57	-0.376330364887977
2216	58-59	-0.37904684408069755
2216	60-61	-0.3924467010261381
2216	62-63	-0.4000484596651219
2216	64-65	-0.43061307773881197
2216	66-67	-0.3811479572204277
2216	68-69	-0.4445574963873895
2216	70-71	-0.45802365773090514
2216	72-73	-0.3487625804673158
2216	74-75	-0.31709396086177577
2216	76-77	-0.3769020982093281
2216	78-79	-0.3544514934796297
2216	80-81	-0.3986022328591545
2216	82-83	-0.29727804581524
2216	84-85	-0.36713701039093394
2216	86-87	-0.36153978620630056
2216	88-89	-0.346636360905336
2216	90-91	-0.34437514724550056
2216	92-93	-0.33734375775404857
2216	94-95	-0.3533412232878703
2216	96-97	-0.30785347257216955
2216	98-99	-0.403429832101466
2216	100	-0.4543627963412362
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1421.0
1	326.0
2	254.0
3	250.0
4	190.0
5	216.0
6	343.0
7	883.0
8	2301.0
9	2860.0
10	3004.0
11	3766.0
12	5033.0
13	6547.0
14	8803.0
15	9819.0
16	10837.0
17	11985.0
18	13645.0
19	15136.0
20	17013.0
21	19227.0
22	21329.0
23	24877.0
24	30480.0
25	37676.0
26	41883.0
27	41089.0
28	44480.0
29	54205.0
30	63435.0
31	73246.0
32	91985.0
33	122360.0
34	179857.0
35	273530.0
36	500485.0
37	1072209.0
38	1369002.0
39	431355.0
40	727.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.166118245271655	20.14836051400712	13.159354719305853	24.52616652141537
2	14.82712494516901	20.78457175367965	40.881725753844684	23.50657754730666
3	17.870487409704758	26.044818584205338	30.620914180968388	25.463779825121513
4	11.999581255902163	17.722040795405285	40.30952747074221	29.968850477950344
5	12.982399746318304	38.54474017716452	35.82083295777003	12.652027118747142
6	29.65668583681646	38.947652393350886	18.76567027791251	12.629991491920148
7	26.271510399969415	32.84737009920826	23.504013421221135	17.377106079601187
8	24.679747199193038	36.27909219514325	21.246862199146342	17.794298406517374
9	26.00221863453208	15.798541208704101	21.00824951559668	37.19099064116714
10-11	24.033897778680796	26.635208484391864	29.524195083588467	19.806698653338874
12-13	24.598787698211908	24.450887488126504	28.770418394395058	22.17990641926653
14-15	22.17383023065703	25.847524070391614	26.12622415196555	25.852421546985806
16-17	22.09459154224793	28.42816479394242	26.93558517782338	22.541658485986275
18-19	21.94517593631261	28.49603582687298	28.041868965644557	21.516919271169858
20-21	23.048618898152323	27.755485138443536	27.294431974923466	21.901463988480675
22-23	22.992058197966603	27.534088765711466	26.94902737167366	22.52482566464827
24-25	22.594917382600155	27.583148404661724	27.489858785295535	22.33207542744259
26-27	22.56540167773593	27.920279582840855	27.048521434811146	22.465797304612064
28-29	22.37281951292614	28.16804607399661	27.033028131656433	22.426106281420815
30-31	22.847077425632474	27.77368727411732	27.005360369752573	22.373874930497635
32-33	22.775419529768715	28.007797911123316	26.665296135242567	22.551486423865406
34-35	22.82150146292651	27.8438773259478	27.18425988603498	22.15036132509071
36-37	22.82321858186811	27.702851882827378	27.104881881349314	22.369047653955203
38-39	22.688823015466618	27.78851192373501	27.242773728414583	22.279891332383787
40-41	22.758284034110808	27.334616606349215	27.42461223525707	22.48248712428291
42-43	22.625828406498968	27.49534141827233	27.189924387509723	22.688905787718983
44-45	22.592312001238916	27.949452143370824	27.071211261952293	22.38702459343797
46-47	22.5734752646606	27.596257421155666	27.00826634397239	22.822000970211338
48-49	22.633985847004855	27.477319899677667	27.28835481341829	22.600339439899187
50-51	22.583728440774284	27.551227308314562	27.09158604418651	22.773458206724644
52-53	22.512727070122185	27.66819917537557	27.043100273336186	22.775973481166066
54-55	22.461347826393872	27.48739830268726	27.14412388677993	22.907129984138937
56-57	22.767673963612367	27.498329063947814	26.974403453564705	22.759593518875114
58-59	22.471662256829454	27.459982641594767	27.35798490442179	22.71037019715399
60-61	22.575949291473023	27.668165867105166	27.063058738173567	22.692826103248244
62-63	22.57391436193518	27.431744330500656	27.05563135476048	22.93870995280368
64-65	22.22966837698928	27.429367730947863	27.224074709672376	23.116889182390484
66-67	22.571242091739784	27.371457066294646	26.97154052751532	23.08576031445025
68-69	22.358054451497814	27.34230221400234	27.15857136557551	23.141071968924333
70-71	22.4579821002699	27.06015848622057	27.172272112653236	23.309587300856297
72-73	22.62519718700866	27.17074059769332	27.23757021291201	22.966492002386012
74-75	22.491573743686484	27.453894955585856	27.123035887472074	22.931495413255586
76-77	22.43856460822678	27.301608748561407	27.054652875862338	23.205173767349475
78-79	22.400019465054253	27.281848554491617	27.12701882049158	23.191113159962555
80-81	22.42916084391566	27.19149593415753	27.317024054867854	23.062319167058952
82-83	22.7064491734951	26.90292947547771	27.566318841826487	22.824302509200706
84-85	22.890956961497356	26.998362051736574	27.105404360331235	23.005276626434835
86-87	22.31028348727823	27.233127844175513	27.347302164641718	23.109286503904542
88-89	22.696178509081236	26.84541788090684	27.235042756858025	23.2233608531539
90-91	22.72074214578596	26.686830240190325	27.390770445893963	23.201657168129753
92-93	22.848798174198762	26.729168834266225	27.29085859098173	23.131174400553284
94-95	22.339700648851913	26.749719363728936	27.517917713622413	23.392662273796738
96-97	22.444689744092663	26.7390771245118	27.368457520624396	23.447775610771146
98-99	22.38852238502486	26.873934449876348	27.277257662836913	23.460285502261875
100	22.812254780068656	26.610856323500716	26.988262510934618	23.58862638549601
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1452.0
1	930.0
2	404.5
3	395.5
4	359.5
5	335.0
6	344.0
7	344.5
8	358.0
9	364.5
10	351.5
11	340.0
12	345.5
13	397.0
14	478.5
15	555.5
16	636.5
17	752.5
18	931.5
19	1205.5
20	1760.5
21	2685.5
22	3933.5
23	5897.0
24	8299.0
25	11840.0
26	17905.5
27	25174.5
28	32853.5
29	41945.5
30	51082.5
31	59899.5
32	72772.0
33	85128.0
34	94702.5
35	106583.0
36	121449.5
37	137725.0
38	145260.0
39	145211.5
40	147447.0
41	149989.5
42	151921.0
43	158572.0
44	167799.0
45	179597.0
46	186892.0
47	192733.5
48	198815.5
49	194928.0
50	187644.0
51	183497.5
52	177742.5
53	169045.0
54	158713.0
55	142989.0
56	128002.5
57	111887.5
58	96226.0
59	83975.5
60	67461.0
61	50745.5
62	39117.5
63	29402.5
64	20383.5
65	13521.5
66	9439.5
67	6884.0
68	5162.0
69	3851.5
70	2828.5
71	2213.5
72	1646.0
73	1147.0
74	895.5
75	665.0
76	483.5
77	335.5
78	190.0
79	123.0
80	92.0
81	74.5
82	55.0
83	36.5
84	28.0
85	28.5
86	25.0
87	24.0
88	21.5
89	12.5
90	12.0
91	13.0
92	11.0
93	10.0
94	7.5
95	6.0
96	6.5
97	7.0
98	6.0
99	5.5
100	6.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09880494410999488
2	0.06529850138962763
3	0.08092326742503206
4	0.08300656956308597
5	0.08406992169605099
6	0.09136147917923973
7	0.08717317383919382
8	0.08958199193631866
9	0.07454315462724191
10-11	0.08149834561939068
12-13	0.07107098439715204
14-15	0.0793607908214916
16-17	0.0808364631692798
18-19	0.0803807408265805
20-21	0.07129884556850169
22-23	0.0761598838906275
24-25	0.07792852060157954
26-27	0.07273111578841376
28-29	0.08108602540456751
30-31	0.08482945893388315
32-33	0.08714062224328673
34-35	0.08924562544527871
36-37	0.08844268607957043
38-39	0.08944093502072126
40-41	0.08586025947094109
42-43	0.09114446853985911
44-45	0.08758549405401699
46-47	0.08501391797735668
48-49	0.0934990339771388
50-51	0.09011366800280117
52-53	0.09543042866762629
54-55	0.0873793339466054
56-57	0.0946600408978251
58-59	0.09842517549107879
60-61	0.09774159197702985
62-63	0.09834922176729559
64-65	0.10088824624804879
66-67	0.10272198615081501
68-69	0.09973808985933154
70-71	0.10207095423267315
72-73	0.09919556326087998
74-75	0.103069203173824
76-77	0.1001504100741547
78-79	0.10737686436552926
80-81	0.10238561965977505
82-83	0.10611820265712167
84-85	0.10305835264185496
86-87	0.09943427496419868
88-89	0.09849027868289299
90-91	0.09880494410999488
92-93	0.09292395578278016
94-95	0.09212101641707188
96-97	0.09062364300534562
98-99	0.10444722073389091
100	0.24804316081204514
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4608069.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.31207604197136
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.67136260057866	36.07700828825867
2	15.759859354507046	16.803816406365065
3	6.734008643408599	10.770119425940747
4	3.436842469588455	7.329008283319055
5	2.070397047386118	5.518858241366085
6	1.2268444657557804	3.924337527002633
7	0.7587534514676249	2.831550519122517
8	0.5286505562138533	2.2546766922002717
9	0.38234358717356987	1.8345177354201714
>10	1.4071345267648518	11.507267468052266
>50	0.019219919900113312	0.6854727321581516
>100	0.004433591782522467	0.3961298273469055
>500	1.4978547285269658E-4	0.06723685344750112
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3020638362837015E-4
2	0.0	0.0	0.0	1.5190744756643184E-4	1.3020638362837015E-4
3	0.0	0.0	0.0	1.5190744756643184E-4	1.3020638362837015E-4
4	0.0	0.0	0.0	1.5190744756643184E-4	1.3020638362837015E-4
5	0.0	0.0	0.0	1.7360851150449353E-4	1.3020638362837015E-4
6	0.0	0.0	0.0	1.7360851150449353E-4	3.4721702300898706E-4
7	0.0	0.0	0.0	1.7360851150449353E-4	3.6891808694704877E-4
8	0.0	0.0	0.0	1.7360851150449353E-4	3.6891808694704877E-4
9	0.0	0.0	0.0	2.170106393806169E-4	7.812383017702209E-4
10-11	0.0	0.0	0.0	2.8211383119480197E-4	9.005941534295602E-4
12-13	0.0	0.0	0.0	2.8211383119480197E-4	0.001052501600995992
14-15	0.0	0.0	0.0	2.8211383119480197E-4	0.0013129143682527322
16-17	0.0	0.0	0.0	2.929643631638328E-4	0.001399718624004979
18-19	0.0	2.170106393806169E-5	0.0	3.255159590709254E-4	0.001486522879757226
20-21	0.0	2.170106393806169E-5	0.0	3.3636649103995625E-4	0.0015190744756643183
22-23	0.0	2.170106393806169E-5	0.0	4.557223426992955E-4	0.00154077553960238
24-25	0.0	2.170106393806169E-5	0.0	7.05284577987005E-4	0.0015516260715714109
26-27	0.0	2.170106393806169E-5	0.0	0.001063352132965023	0.0016601313912617194
28-29	2.170106393806169E-5	2.170106393806169E-5	0.0	0.003363664910399562	0.001692682987168812
30-31	2.170106393806169E-5	2.170106393806169E-5	0.0	0.01128455324779208	0.0017360851150449352
32-33	2.170106393806169E-5	2.170106393806169E-5	0.0	0.03119527941096368	0.0020833021380539224
34-35	2.170106393806169E-5	2.170106393806169E-5	0.0	0.06241225988586543	0.0022352095856203544
36-37	2.170106393806169E-5	2.170106393806169E-5	0.0	0.11521094844716953	0.002473921288939033
38-39	2.170106393806169E-5	2.170106393806169E-5	0.0	0.19494065735560817	0.002593277140598372
40-41	2.170106393806169E-5	2.170106393806169E-5	0.0	0.2850651758903784	0.0027126329922577113
42-43	2.170106393806169E-5	2.170106393806169E-5	0.0	0.38072346572935434	0.0028536899078551127
44-45	2.170106393806169E-5	2.170106393806169E-5	0.0	0.4985927945089364	0.002886241503762205
46-47	2.170106393806169E-5	2.170106393806169E-5	0.0	0.6454330436458309	0.002897092035731236
48-49	2.170106393806169E-5	2.170106393806169E-5	0.0	0.8053481838054074	0.0029296436316383285
50-51	2.170106393806169E-5	2.170106393806169E-5	0.0	0.975799190506913	0.0029947468234525136
52-53	2.170106393806169E-5	4.340212787612338E-5	0.0	1.141475963142045	0.0029947468234525136
54-55	2.170106393806169E-5	8.680425575224676E-5	0.0	1.330003956103956	0.003027298419359606
56-57	2.170106393806169E-5	8.680425575224676E-5	0.0	1.556508810957475	0.003092401611173791
58-59	2.170106393806169E-5	8.680425575224676E-5	0.0	1.8131021909611162	0.0031249532070808834
60-61	2.170106393806169E-5	8.680425575224676E-5	0.0	2.087099824243083	0.0031249532070808834
62-63	2.170106393806169E-5	9.765478772127761E-5	0.0	2.363940296900936	0.0031249532070808834
64-65	2.170106393806169E-5	1.0850531969030845E-4	0.0	2.655146873885786	0.003168355334957007
66-67	2.170106393806169E-5	1.0850531969030845E-4	0.0	2.991741226097092	0.003168355334957007
68-69	2.170106393806169E-5	1.0850531969030845E-4	0.0	3.3648693194481245	0.0031900563988950686
70-71	2.170106393806169E-5	1.0850531969030845E-4	0.0	3.7708311225374445	0.003298561718585377
72-73	2.170106393806169E-5	1.0850531969030845E-4	0.0	4.186471600143141	0.0033636649103995623
74-75	2.170106393806169E-5	1.0850531969030845E-4	0.0	4.603945817651602	0.0034287681022137475
76-77	2.170106393806169E-5	1.0850531969030845E-4	0.0	5.067621600284197	0.0035589744858421173
78-79	2.170106393806169E-5	1.0850531969030845E-4	0.0	5.5808083602914795	0.003667479805532426
80-81	2.170106393806169E-5	1.0850531969030845E-4	0.0	6.134532707735062	0.003667479805532426
82-83	2.170106393806169E-5	1.0850531969030845E-4	0.0	6.702286793014601	0.0037434335293156417
84-85	2.170106393806169E-5	1.0850531969030845E-4	0.0	7.26948750116372	0.0038410883170369196
86-87	2.170106393806169E-5	1.0850531969030845E-4	0.0	7.860364070069263	0.003862789380974981
88	2.170106393806169E-5	1.0850531969030845E-4	0.0	8.33197159157122	0.0039061915088511043
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3665	0.0	38.365013	1
GTATCAA	5880	0.0	33.749958	1
CAACGCA	5760	0.0	31.502655	5
ATCAACG	6210	0.0	29.448872	3
AACGCAG	6855	0.0	27.627296	6
TATCAAC	7280	0.0	26.541233	2
TCAACGC	7230	0.0	25.358732	4
GTACATG	12495	0.0	25.328934	1
ACGCAGA	7340	0.0	24.711763	7
TACATGG	12555	0.0	24.22692	2
CGCAGAG	7455	0.0	24.141203	8
ACATGGG	12355	0.0	23.667822	3
CGTTAAC	460	0.0	21.468395	1
CATGGGG	9600	0.0	21.301945	4
TGATCGC	1320	0.0	21.002306	9
GTGATCG	1270	0.0	20.720112	8
TATTCGC	370	0.0	20.319225	9
GTACTAG	2150	0.0	19.904032	1
GTATAAG	2745	0.0	19.701242	1
ATGGGGG	6700	0.0	18.452843	5
>>END_MODULE