FastQCFastQC Report
Mon 16 Jan 2017
ERR1146633_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146633_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4608069
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99630.2162077000149086No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94350.20474953825561207No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49770.10800619521973304No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67750.060.12041
GTATCAA133900.043.76741
CAACGCA164700.034.9865
ATCAACG166250.034.655233
TATCAAC173400.033.769462
AACGCAG177650.033.015556
TCAACGC179650.032.3055884
ACGCAGA192050.029.9771887
CGCAGAG196250.029.2877458
TGATCGC14550.026.1632169
GTGATCG14400.026.1093838
GCAGAGT222050.025.6943159
GAGTACT148200.023.59409312-13
GTGGTAT21850.023.4632571
GTACATG125100.022.595941
CAGAGTA213700.022.43207710-11
AGAGTAC202150.022.2258310-11
TACATGG125350.021.7999552
ACATGGG123300.021.2083133
GTATAAG27250.021.057491
Produced by FastQC (version 0.11.5)