##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146633_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4608069
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.35653632790655	33.0	31.0	34.0	30.0	34.0
2	32.45134176593276	34.0	31.0	34.0	30.0	34.0
3	32.55126279576109	34.0	31.0	34.0	30.0	34.0
4	36.03769648414553	37.0	35.0	37.0	35.0	37.0
5	36.07567746923928	37.0	35.0	37.0	35.0	37.0
6	36.13611992355149	37.0	36.0	37.0	35.0	37.0
7	36.099902149902704	37.0	36.0	37.0	35.0	37.0
8	36.10653659916985	37.0	36.0	37.0	35.0	37.0
9	37.90359454252964	39.0	38.0	39.0	35.0	39.0
10-11	37.847873588698434	39.0	38.0	39.0	35.0	39.0
12-13	37.81776151789394	39.0	38.0	39.0	35.0	39.0
14-15	39.1844748852502	40.0	39.0	41.0	36.0	41.0
16-17	39.13541409644691	40.0	39.0	41.0	36.0	41.0
18-19	39.22790923920627	40.0	39.0	41.0	36.0	41.0
20-21	39.28037991618615	41.0	39.0	41.0	36.0	41.0
22-23	39.2481845432436	40.5	39.0	41.0	36.0	41.0
24-25	39.23852084246134	41.0	39.0	41.0	36.0	41.0
26-27	39.16316118530343	41.0	39.0	41.0	35.5	41.0
28-29	39.10878200825552	40.0	39.0	41.0	35.0	41.0
30-31	38.97902342608151	40.0	38.5	41.0	35.0	41.0
32-33	38.885512782035164	40.0	38.0	41.0	35.0	41.0
34-35	38.81906369023554	40.0	38.0	41.0	35.0	41.0
36-37	38.75939932757083	40.0	38.0	41.0	35.0	41.0
38-39	38.67529045246501	40.0	38.0	41.0	35.0	41.0
40-41	38.586989257322315	40.0	38.0	41.0	35.0	41.0
42-43	38.48949429793694	40.0	38.0	41.0	34.5	41.0
44-45	38.356292190937246	40.0	38.0	41.0	34.0	41.0
46-47	38.147047928318784	40.0	38.0	41.0	33.5	41.0
48-49	37.89000924248313	40.0	38.0	41.0	33.0	41.0
50-51	37.793294653356966	40.0	37.0	41.0	33.0	41.0
52-53	37.69544086687938	40.0	37.0	41.0	33.0	41.0
54-55	37.54290690959705	40.0	37.0	41.0	33.0	41.0
56-57	37.39757662048897	40.0	36.0	41.0	33.0	41.0
58-59	37.219575922148735	39.5	36.0	41.0	32.0	41.0
60-61	37.02518592929056	39.0	35.5	41.0	32.0	41.0
62-63	36.789648766110055	39.0	35.0	41.0	32.0	41.0
64-65	36.51882187528008	38.5	35.0	41.0	31.0	41.0
66-67	36.256685718030695	37.5	35.0	40.0	31.0	41.0
68-69	35.96714697631481	37.0	35.0	40.0	31.0	41.0
70-71	35.66456361656043	36.5	35.0	39.5	31.0	41.0
72-73	35.339475819481	36.0	35.0	39.0	31.0	41.0
74-75	35.01844188965052	36.0	35.0	39.0	30.0	40.5
76-77	34.709320541858204	35.0	35.0	37.5	30.0	39.5
78-79	34.4131877148541	35.0	34.0	37.0	30.0	39.0
80-81	34.144587244678846	35.0	34.0	37.0	30.0	39.0
82-83	33.88271508087227	35.0	34.0	36.0	30.0	38.0
84-85	33.666586915256694	35.0	34.0	36.0	30.0	37.0
86-87	33.47597236499714	35.0	34.0	35.5	29.0	37.0
88-89	33.279449917091085	35.0	34.0	35.0	29.0	36.5
90-91	33.19163298119017	35.0	34.0	35.0	29.0	36.0
92-93	33.091679508271255	35.0	34.0	35.0	29.0	36.0
94-95	33.017313217315106	35.0	34.0	35.0	29.0	36.0
96-97	32.963066308251896	35.0	34.0	35.0	29.0	35.5
98-99	32.89814920306098	35.0	34.0	35.0	29.0	35.0
100	32.16972988034684	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.32578309260885163
1101	2	0.3085400683088011
1101	3	0.33802936461471234
1101	4	0.2001126086702385
1101	5	0.20544404197055854
1101	6	0.22390598574173026
1101	7	0.22503074612745166
1101	8	0.2405705277739898
1101	9	0.2921992976640624
1101	10-11	0.2828168382313123
1101	12-13	0.3249278709387795
1101	14-15	0.45619915168454384
1101	16-17	0.5101028737459572
1101	18-19	0.4338448358065179
1101	20-21	0.3857024870637531
1101	22-23	0.4176864070265651
1101	24-25	0.3885609382294888
1101	26-27	0.3841432470741921
1101	28-29	0.4214916810648788
1101	30-31	0.4492020947383111
1101	32-33	0.4415091942669207
1101	34-35	0.4512214337181675
1101	36-37	0.4787065781127282
1101	38-39	0.4525833423980288
1101	40-41	0.38717056032619723
1101	42-43	0.4210563942553023
1101	44-45	0.4298472112505962
1101	46-47	0.4415449519691279
1101	48-49	0.4766955242660771
1101	50-51	0.44694466474263095
1101	52-53	0.4278177589366692
1101	54-55	0.42284011310630376
1101	56-57	0.42957365916713997
1101	58-59	0.4132951536204601
1101	60-61	0.3827730794328943
1101	62-63	0.3968364603504142
1101	64-65	0.39605064350663355
1101	66-67	0.39546375458935756
1101	68-69	0.2931461886388931
1101	70-71	0.33204447168695594
1101	72-73	0.3311623636635801
1101	74-75	0.29817012733261805
1101	76-77	0.2809697887435547
1101	78-79	0.20666301715040447
1101	80-81	0.1412972720312311
1101	82-83	0.18143575484344154
1101	84-85	0.19630176924696485
1101	86-87	0.22502204177590102
1101	88-89	0.26787183932797376
1101	90-91	0.23969832998243135
1101	92-93	0.23631239750721988
1101	94-95	0.29224510053281705
1101	96-97	0.21432716296132526
1101	98-99	0.2951080746020338
1101	100	0.37079944100054263
1102	1	0.2336262378918761
1102	2	0.2522279028969052
1102	3	0.26708023744608056
1102	4	0.18823386899302363
1102	5	0.20116016550847604
1102	6	0.18059844733495112
1102	7	0.18851711517977066
1102	8	0.21919376643190702
1102	9	0.2585422667481936
1102	10-11	0.3099939607967599
1102	12-13	0.27659464081699525
1102	14-15	0.36050344867996387
1102	16-17	0.4451811305637108
1102	18-19	0.3490560632216031
1102	20-21	0.36012062270374656
1102	22-23	0.3827428427046442
1102	24-25	0.36023842334329004
1102	26-27	0.358395917253695
1102	28-29	0.3688858054900166
1102	30-31	0.4425187060943969
1102	32-33	0.4193635210856428
1102	34-35	0.41675544317529756
1102	36-37	0.3949158015177403
1102	38-39	0.3894863964989028
1102	40-41	0.41845277306357076
1102	42-43	0.41215631719637
1102	44-45	0.4467465247146549
1102	46-47	0.4299542947810906
1102	48-49	0.49906138208969253
1102	50-51	0.4059429519995419
1102	52-53	0.4530728009346703
1102	54-55	0.4434910385044901
1102	56-57	0.4311858065354883
1102	58-59	0.4546338250665869
1102	60-61	0.3636135182986493
1102	62-63	0.36526090503105024
1102	64-65	0.38765337445584436
1102	66-67	0.397698696948126
1102	68-69	0.42609782120395323
1102	70-71	0.4466360630106081
1102	72-73	0.4469716096102232
1102	74-75	0.37437593537357117
1102	76-77	0.3442905018347133
1102	78-79	0.3389618579794842
1102	80-81	0.30766914603625395
1102	82-83	0.28197021723720894
1102	84-85	0.26251986941402805
1102	86-87	0.2993779604693074
1102	88-89	0.3341982974072266
1102	90-91	0.24230218966226147
1102	92-93	0.2915584274023999
1102	94-95	0.2713708628695457
1102	96-97	0.2566487325093334
1102	98-99	0.3089885639282812
1102	100	0.2273339348980059
1103	1	0.2460760953779797
1103	2	0.22586318723831056
1103	3	0.2383597478260242
1103	4	0.19201461809004883
1103	5	0.1521569327763359
1103	6	0.2238521980785393
1103	7	0.20976369321359556
1103	8	0.2210070931602104
1103	9	0.23680134858935986
1103	10-11	0.2598962230976838
1103	12-13	0.26316183661816694
1103	14-15	0.3657528297036521
1103	16-17	0.4536571279562054
1103	18-19	0.3542082471368815
1103	20-21	0.32208586523618976
1103	22-23	0.3574475682642415
1103	24-25	0.39616670651144403
1103	26-27	0.34764613548304624
1103	28-29	0.3526714712317869
1103	30-31	0.3546998627203237
1103	32-33	0.38226109138727793
1103	34-35	0.33714107324900766
1103	36-37	0.34937065624573904
1103	38-39	0.3328717564956989
1103	40-41	0.34389953301980825
1103	42-43	0.4030855335659851
1103	44-45	0.41060820296360845
1103	46-47	0.4058497668264849
1103	48-49	0.38299738663982197
1103	50-51	0.41041303609469537
1103	52-53	0.33711506704266014
1103	54-55	0.3452223046628262
1103	56-57	0.323784448768329
1103	58-59	0.35173269341922264
1103	60-61	0.36120510500431635
1103	62-63	0.343244422148814
1103	64-65	0.3546620002077745
1103	66-67	0.28487524217191407
1103	68-69	0.2914564823563808
1103	70-71	0.2859732835730213
1103	72-73	0.27813220533457894
1103	74-75	0.2223562176842151
1103	76-77	0.20863443609627552
1103	78-79	0.20414884064378214
1103	80-81	0.2484843523818938
1103	82-83	0.23230784613668476
1103	84-85	0.2154594867479247
1103	86-87	0.21597658916007845
1103	88-89	0.2831609967609481
1103	90-91	0.23497827833266882
1103	92-93	0.27502470781431754
1103	94-95	0.2626549851976705
1103	96-97	0.2671104449847803
1103	98-99	0.1809261041927286
1103	100	0.1311959473302835
1104	1	0.2201119458770151
1104	2	0.22070376287405935
1104	3	0.2182082818548281
1104	4	0.16892293678492365
1104	5	0.13349985205515935
1104	6	0.18729944021046663
1104	7	0.18775418811493694
1104	8	0.19913486034056405
1104	9	0.23894446955646487
1104	10-11	0.21872835813553593
1104	12-13	0.22104923768473128
1104	14-15	0.36733421943146993
1104	16-17	0.43293164160836284
1104	18-19	0.3277736720725031
1104	20-21	0.3524941459393318
1104	22-23	0.3642631499909541
1104	24-25	0.3492887200222796
1104	26-27	0.32090105052353124
1104	28-29	0.35313231388276733
1104	30-31	0.37532469580376926
1104	32-33	0.42256302973984106
1104	34-35	0.3596849335724457
1104	36-37	0.3960786081386374
1104	38-39	0.41582296302490107
1104	40-41	0.4021946902786624
1104	42-43	0.4219078378168888
1104	44-45	0.40222421354461346
1104	46-47	0.49248417163141056
1104	48-49	0.5611089007823438
1104	50-51	0.5320411648110195
1104	52-53	0.5181318116982823
1104	54-55	0.5429659772082402
1104	56-57	0.5066304315199304
1104	58-59	0.4551526217667501
1104	60-61	0.4158710316034373
1104	62-63	0.44016686132399485
1104	64-65	0.4264763198139221
1104	66-67	0.4242413025762204
1104	68-69	0.43392966811234146
1104	70-71	0.39421583547985506
1104	72-73	0.3613265537537913
1104	74-75	0.3265781691270533
1104	76-77	0.3191425558430936
1104	78-79	0.32072086526451926
1104	80-81	0.3112292624696025
1104	82-83	0.29122501677564827
1104	84-85	0.31841010078382226
1104	86-87	0.3120335070057365
1104	88-89	0.353051308104412
1104	90-91	0.36118675157260327
1104	92-93	0.3140919669951998
1104	94-95	0.362656180120176
1104	96-97	0.3546305566810162
1104	98-99	0.3660062651898386
1104	100	0.4889718780768604
1105	1	0.17995241781346039
1105	2	0.15498272808588354
1105	3	0.14451091854898124
1105	4	0.15195251113112818
1105	5	0.12437547277119876
1105	6	0.15938464219669868
1105	7	0.1397774325405976
1105	8	0.1457796545384511
1105	9	0.16433236878632584
1105	10-11	0.1915495982618154
1105	12-13	0.1777212029898152
1105	14-15	0.2794955315765293
1105	16-17	0.3401980033476093
1105	18-19	0.26551500668580985
1105	20-21	0.27222369363929033
1105	22-23	0.2562538739020255
1105	24-25	0.25681717659244896
1105	26-27	0.23733285468789234
1105	28-29	0.25218574875106015
1105	30-31	0.2501807414964432
1105	32-33	0.20370029508545429
1105	34-35	0.19656000016006203
1105	36-37	0.21553946850700356
1105	38-39	0.1984145728396527
1105	40-41	0.1706277663551532
1105	42-43	0.20122227299673767
1105	44-45	0.18397328236322608
1105	46-47	0.18810095009060745
1105	48-49	0.2843837206327464
1105	50-51	0.2325792487466174
1105	52-53	0.2013299763699905
1105	54-55	0.23874495507499205
1105	56-57	0.18385954600373822
1105	58-59	0.1683114994648136
1105	60-61	0.19944225864550447
1105	62-63	0.18574919527380018
1105	64-65	0.2002539130315526
1105	66-67	0.2201433429439703
1105	68-69	0.17226264046333029
1105	70-71	0.2064048615121692
1105	72-73	0.14043242550673796
1105	74-75	0.1367897062719905
1105	76-77	0.1336295499143958
1105	78-79	0.1512884467209119
1105	80-81	0.15821493300120437
1105	82-83	0.12970591349311889
1105	84-85	0.13632601646163067
1105	86-87	0.1346121423610782
1105	88-89	0.18398308229267712
1105	90-91	0.19179976379516717
1105	92-93	0.21171573260918564
1105	94-95	0.1729899734353495
1105	96-97	0.1396639675191551
1105	98-99	0.1667811191973101
1105	100	0.18420445485541848
1106	1	0.0821742649246886
1106	2	0.08231691220321835
1106	3	0.11744447781236289
1106	4	0.10384201140191607
1106	5	0.10843291542209244
1106	6	0.08933726553553356
1106	7	0.10165148771099553
1106	8	0.12051275029462971
1106	9	0.12008616860575927
1106	10-11	0.09711766239327346
1106	12-13	0.11220490465027666
1106	14-15	0.224921400491219
1106	16-17	0.31425216322378446
1106	18-19	0.2123411133958868
1106	20-21	0.16841503450082485
1106	22-23	0.18930612068840702
1106	24-25	0.15643508998644506
1106	26-27	0.15034102087167867
1106	28-29	0.15897436270589083
1106	30-31	0.14625441342264622
1106	32-33	0.1415276400176353
1106	34-35	0.1958453876186823
1106	36-37	0.14644454484285063
1106	38-39	0.10373163125812113
1106	40-41	0.0960220082890757
1106	42-43	0.10481720900777702
1106	44-45	0.08355206766351841
1106	46-47	0.11085253347373936
1106	48-49	0.19168394123263965
1106	50-51	0.08966665592651424
1106	52-53	0.08918893096648617
1106	54-55	0.13500922671804005
1106	56-57	0.12301103117167855
1106	58-59	0.11044737056636222
1106	60-61	0.08583677406096513
1106	62-63	0.0819312374310357
1106	64-65	0.11035037499080147
1106	66-67	0.08926126904066933
1106	68-69	0.08240939133355596
1106	70-71	0.10983977441307502
1106	72-73	0.13171946841938365
1106	74-75	0.08672209022184774
1106	76-77	0.07169826975869142
1106	78-79	0.059841394930856495
1106	80-81	0.08564140438654277
1106	82-83	0.012150377278231872
1106	84-85	-0.04481192481140539
1106	86-87	0.06582570083976691
1106	88-89	0.10949333267902972
1106	90-91	0.04051652243953896
1106	92-93	0.08512834356412924
1106	94-95	0.057700593132082645
1106	96-97	0.06506545627492244
1106	98-99	0.09008811039254994
1106	100	0.09566031859106516
1107	1	0.14402014719343015
1107	2	0.09979349448045838
1107	3	0.10236512727524882
1107	4	0.07544512930130054
1107	5	0.06639626642524377
1107	6	0.09039077063705747
1107	7	0.07118481807112431
1107	8	0.09978182394237933
1107	9	0.13019720392707512
1107	10-11	0.0655679860979177
1107	12-13	0.13772038380449203
1107	14-15	0.1845999902800628
1107	16-17	0.2564184116252619
1107	18-19	0.14643815668330973
1107	20-21	0.1499778539689487
1107	22-23	0.15182448857486008
1107	24-25	0.1353985344495996
1107	26-27	0.11928275288255463
1107	28-29	0.11290232751699136
1107	30-31	0.12169058986452086
1107	32-33	0.08696566837347319
1107	34-35	0.05104800394556008
1107	36-37	0.04800072876614081
1107	38-39	0.11002379413574204
1107	40-41	0.13902399192285486
1107	42-43	0.12771407952672575
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2216	40-41	-0.5353146876291959
2216	42-43	-0.5644729002315358
2216	44-45	-0.5506352127317271
2216	46-47	-0.5318948563782371
2216	48-49	-0.4543906870237393
2216	50-51	-0.4791810656449371
2216	52-53	-0.4806212327429691
2216	54-55	-0.41190100248135764
2216	56-57	-0.4518290013158932
2216	58-59	-0.4915612312576769
2216	60-61	-0.5363906494687427
2216	62-63	-0.5573141911551787
2216	64-65	-0.4604162833323997
2216	66-67	-0.4563944257654171
2216	68-69	-0.4065880902094108
2216	70-71	-0.37154617044675575
2216	72-73	-0.3530376413256633
2216	74-75	-0.38741713221128293
2216	76-77	-0.36719326966522914
2216	78-79	-0.3089958947158209
2216	80-81	-0.3581748981182429
2216	82-83	-0.37482091382556604
2216	84-85	-0.32511295932508233
2216	86-87	-0.28269285524748966
2216	88-89	-0.1813711766725845
2216	90-91	-0.22188180868843688
2216	92-93	-0.24409351103482635
2216	94-95	-0.23709368962262545
2216	96-97	-0.2792628477849348
2216	98-99	-0.29254983583786043
2216	100	-0.41250219968835466
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	25.0
8	158.0
9	282.0
10	243.0
11	187.0
12	179.0
13	273.0
14	539.0
15	1024.0
16	1860.0
17	3440.0
18	5691.0
19	8929.0
20	12728.0
21	18389.0
22	26702.0
23	30461.0
24	28900.0
25	25236.0
26	24669.0
27	28323.0
28	35599.0
29	44480.0
30	55256.0
31	69709.0
32	89679.0
33	117470.0
34	168873.0
35	257320.0
36	456035.0
37	1056442.0
38	1511456.0
39	526239.0
40	1273.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.72864101320434	20.167818479130112	13.460200891718777	24.643339615946772
2	14.976463334734543	20.993260078252277	40.408019502087384	23.622257084925792
3	17.616073426238803	26.262067732293875	31.016787350319103	25.105071491148212
4	12.058982857279332	18.154869624783828	39.64982863792937	30.13631888000747
5	12.57653913237095	38.32443889749361	35.894088829773494	13.204933140361947
6	29.241872712187767	39.21663095579903	18.328757437593875	13.212738894419317
7	25.463361217759804	33.474766431398145	23.459093919620976	17.60277843122107
8	24.930507587037226	35.686624536111424	21.032357021625383	18.350510855225966
9	26.069120118748323	14.991064579167176	21.118235094340033	37.82158020774447
10-11	24.016463739885673	26.35215077214606	29.400067186537694	20.23131830143058
12-13	24.993581910340318	24.263677475315582	28.98423396003836	21.75850665430574
14-15	22.26120459212173	25.35380352625158	27.007338870580412	25.37765301104628
16-17	21.508062615531315	28.092604303157643	27.7277217430857	22.671611338225343
18-19	21.318751501444318	27.361800392051933	29.955482379472677	21.363965727031076
20-21	22.33685235047523	26.63587639694166	29.547016286146604	21.480254966436508
22-23	22.216138690631585	26.467952194292227	29.269277434864797	22.046631680211387
24-25	21.884155814507118	26.497096723161047	29.77377291876489	21.84497454356695
26-27	21.93298978300731	26.88531406448074	29.27025557509741	21.911440577414545
28-29	21.69419176259896	27.0728781642196	29.37141220262073	21.861517870560707
30-31	22.172098689263887	26.65393100935437	29.239292591599565	21.93467770978218
32-33	22.19772891485932	26.896049455937167	28.890684293167528	22.01553733603599
34-35	22.220328198709133	26.830606879855353	29.28556516817699	21.663499753258527
36-37	22.23299560303559	26.66466004009709	29.156985724269763	21.94535863259756
38-39	22.13810092537405	26.740893638136136	29.262796443207762	21.85820899328205
40-41	22.266421501183583	26.398011824539868	29.274384098762944	22.0611825755136
42-43	22.250220211546313	26.544915017548565	28.89555256225547	22.309312208649654
44-45	22.243875169648913	27.002801187314535	28.656213501340915	22.097110141695634
46-47	22.387555394678333	26.72882068389167	28.309656387523713	22.573967533906288
48-49	22.581684013800164	26.819734630306623	28.252430197336754	22.34615115855646
50-51	22.453917248200927	27.007039174109586	27.894883952475535	22.644159625213945
52-53	22.292272241019614	27.168373478059564	27.870524156984622	22.6688301239362
54-55	22.322281309576383	27.010170234513257	27.857187034442948	22.81036142146741
56-57	22.591923435033028	26.965748388069123	27.716769343631665	22.725558833266188
58-59	22.203018996003056	27.00497089640631	28.135521054369967	22.656489053220668
60-61	22.331828969782052	27.19893134596611	27.83385428879245	22.63538539545939
62-63	22.289260621969508	26.974241087157047	27.819196188066748	22.917302102806705
64-65	21.9552874163367	26.915201080542893	27.971492955594705	23.158018547525703
66-67	22.22511212290187	26.862209137735743	27.796692340486434	23.115986398875954
68-69	22.043983125681297	26.767353544928557	27.972911592554517	23.215751736835628
70-71	22.163940659289626	26.474603718527685	27.95183878813316	23.409616834049533
72-73	22.24858138501528	26.557839722955713	27.95104740688737	23.24253148514164
74-75	22.09928708931897	26.885384045214995	27.770855548156494	23.244473317309545
76-77	22.05015232594932	26.77120421052449	27.619207696724875	23.55943576680132
78-79	21.952842085526346	26.798299134528225	27.68077392243545	23.568084857509984
80-81	21.898998246317994	26.72006126710849	27.842973303712505	23.53796718286101
82-83	22.151966928206175	26.42762843298408	28.049295627171272	23.371109011638474
84-85	22.34111096619541	26.5032466059887	27.579788209441624	23.575854218374264
86-87	21.711157288568952	26.76108221915538	27.79593011692426	23.73183037535141
88-89	22.010021985347876	26.391282335399058	27.700746234485642	23.897949444767427
90-91	21.91513036647141	26.208567908347696	27.892288904188145	23.98401282099275
92-93	21.995075807841708	26.328217339792587	27.673076695632908	24.003630156732797
94-95	21.455704766221213	26.314622183483365	27.928434579948824	24.3012384703466
96-97	21.55012975672012	26.24770605070934	27.792536381740074	24.409627810830468
98-99	21.427146131992323	26.38094071403313	27.66500505038613	24.526908103588415
100	21.749111823095618	26.073407856437512	27.48091768833162	24.69656263213525
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	99.0
1	65.5
2	109.5
3	201.0
4	149.0
5	117.5
6	245.5
7	647.0
8	1240.5
9	1593.0
10	1402.0
11	1168.5
12	1306.5
13	1579.0
14	1922.0
15	2352.5
16	2893.0
17	3419.5
18	4068.5
19	4868.5
20	5826.0
21	7006.0
22	8412.5
23	10363.5
24	12816.0
25	16292.5
26	22102.5
27	28781.5
28	35691.0
29	44304.0
30	52786.0
31	60667.0
32	72926.5
33	84446.5
34	93046.5
35	104553.0
36	118732.0
37	134332.0
38	142104.0
39	142419.0
40	144728.0
41	147140.0
42	149172.5
43	155331.0
44	164237.0
45	176715.0
46	185070.0
47	190618.0
48	196032.5
49	193092.5
50	186093.5
51	181581.5
52	175560.5
53	167373.0
54	158318.0
55	142573.5
56	127320.0
57	111756.5
58	96330.5
59	83970.0
60	67153.0
61	50087.5
62	38016.0
63	28336.0
64	19495.0
65	12818.5
66	8948.0
67	6484.5
68	4753.0
69	3364.5
70	2454.0
71	1863.0
72	1292.0
73	930.5
74	735.5
75	515.0
76	344.0
77	216.0
78	95.5
79	53.5
80	37.5
81	27.5
82	14.0
83	6.0
84	3.5
85	2.5
86	1.0
87	0.5
88	0.0
89	0.5
90	0.5
91	0.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.002473921288939033
2	0.003298561718585377
3	3.4721702300898706E-4
4	0.05043327259205537
5	0.0028645404398241433
6	0.004947842577878066
7	2.170106393806169E-5
8	2.604127672567403E-4
9	8.680425575224676E-5
10-11	6.510319181418508E-5
12-13	0.0
14-15	6.510319181418508E-5
16-17	2.387117033186786E-4
18-19	1.3020638362837015E-4
20-21	2.4956223528770945E-4
22-23	0.0
24-25	0.0
26-27	2.2786117134964773E-4
28-29	3.255159590709254E-5
30-31	2.0616010741158607E-4
32-33	1.6275797953546267E-4
34-35	1.5190744756643184E-4
36-37	7.595372378321592E-5
38-39	8.788930894914985E-4
40-41	5.425265984515423E-4
42-43	0.0
44-45	4.991244705754189E-4
46-47	0.0
48-49	1.5190744756643184E-4
50-51	0.0
52-53	2.7126329922577116E-4
54-55	3.038148951328637E-4
56-57	1.8445904347352439E-4
58-59	0.0010199500050888995
60-61	6.401813861728199E-4
62-63	2.929643631638329E-4
64-65	3.2551595907092534E-4
66-67	0.0014648218158191642
68-69	0.0026041276725674027
70-71	0.003689180869470487
72-73	0.003070700547235729
74-75	0.0027885867160409275
76-77	0.004058098956417536
78-79	0.0013454659641598248
80-81	0.0010850531969030847
82-83	7.269856419250667E-4
84-85	5.642276623896039E-4
86-87	1.0850531969030845E-5
88-89	0.0
90-91	3.255159590709254E-5
92-93	7.595372378321592E-5
94-95	1.193558516593393E-4
96-97	4.882739386063881E-4
98-99	1.193558516593393E-4
100	0.0740440301566665
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4608069.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.00368544795882
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.40757276912326	33.87030952878799
2	16.386141450729973	16.715072085175827
3	6.95765482044042	10.64598113751653
4	3.581712006254472	7.307220501287226
5	2.0458943222128982	5.21740752349558
6	1.3044250090981755	3.99182897126965
7	0.8128917283309938	2.9022331810529125
8	0.5730206050951298	2.338093015797683
9	0.3968112179193014	1.8214951086879743
>10	1.4982908784290832	11.74393726606882
>50	0.02309636867255155	0.7949699944653885
>100	0.010950836091000341	1.0474484880928654
>500	9.227925616192348E-4	0.3611517373334749
>1k	4.6139628080805426E-4	0.4820324556708845
>5k	1.5379876026935143E-4	0.7608190052973
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9963	0.2162077000149086	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9435	0.20474953825561207	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4977	0.10800619521973304	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7360851150449353E-4
2	0.0	0.0	0.0	1.0850531969030845E-4	1.7360851150449353E-4
3	0.0	0.0	0.0	1.0850531969030845E-4	1.7360851150449353E-4
4	0.0	0.0	0.0	2.170106393806169E-4	1.7360851150449353E-4
5	0.0	2.170106393806169E-5	0.0	2.3871170331867862E-4	1.7360851150449353E-4
6	0.0	2.170106393806169E-5	0.0	2.3871170331867862E-4	3.038148951328637E-4
7	0.0	2.170106393806169E-5	0.0	2.3871170331867862E-4	3.038148951328637E-4
8	0.0	2.170106393806169E-5	0.0	2.3871170331867862E-4	3.038148951328637E-4
9	0.0	2.170106393806169E-5	0.0	2.3871170331867862E-4	6.076297902657274E-4
10-11	0.0	2.170106393806169E-5	0.0	4.340212787612338E-4	7.920888337392517E-4
12-13	0.0	2.170106393806169E-5	0.0	4.340212787612338E-4	9.765478772127761E-4
14-15	0.0	2.170106393806169E-5	0.0	4.340212787612338E-4	0.0012803627723456399
16-17	0.0	2.170106393806169E-5	0.0	4.4487181073026466E-4	0.0012803627723456399
18-19	0.0	2.170106393806169E-5	0.0	4.665728746683264E-4	0.001334615432190794
20-21	0.0	2.170106393806169E-5	0.0	4.8827393860638804E-4	0.0013671670280978865
22-23	0.0	2.170106393806169E-5	0.0	6.076297902657274E-4	0.0013671670280978865
24-25	0.0	2.170106393806169E-5	0.0	8.571920255534369E-4	0.0013671670280978865
26-27	0.0	2.170106393806169E-5	0.0	0.0012369606444695164	0.0015082239436952877
28-29	0.0	2.170106393806169E-5	0.0	0.0034830207620589015	0.00154077553960238
30-31	0.0	2.170106393806169E-5	0.0	0.011458161759296573	0.00154077553960238
32-33	0.0	2.170106393806169E-5	0.0	0.0314122900503443	0.0020181989462397373
34-35	0.0	2.170106393806169E-5	0.0	0.06281372956871957	0.0021592558618371387
36-37	0.0	2.170106393806169E-5	0.0	0.11578602664152815	0.0023762665012177554
38-39	0.0	2.170106393806169E-5	0.0	0.19559168927375004	0.002539024480753218
40-41	0.0	2.170106393806169E-5	0.0	0.28582471312821056	0.002604127672567403
42-43	0.0	2.170106393806169E-5	0.0	0.38166746201066	0.0027126329922577113
44-45	0.0	2.170106393806169E-5	0.0	0.499515089726304	0.002799437248009958
46-47	0.0	2.170106393806169E-5	0.0	0.645877915456561	0.002799437248009958
48-49	0.0	2.170106393806169E-5	0.0	0.8057822050841686	0.0028319888439170505
50-51	0.0	2.170106393806169E-5	0.0	0.9766346814685283	0.0029296436316383285
52-53	0.0	2.170106393806169E-5	0.0	1.1430709913414925	0.0029296436316383285
54-55	0.0	2.170106393806169E-5	0.0	1.3325972332445544	0.00295134469557639
56-57	0.0	2.170106393806169E-5	0.0	1.5609032764049324	0.0030055973554215442
58-59	0.0	2.170106393806169E-5	0.0	1.8193737984392162	0.0030381489513286366
60-61	0.0	2.170106393806169E-5	0.0	2.095508986519082	0.0030381489513286366
62-63	0.0	2.170106393806169E-5	0.0	2.3746714730183074	0.0030381489513286366
64-65	0.0	2.170106393806169E-5	0.0	2.6688727968266104	0.003092401611173791
66-67	0.0	2.170106393806169E-5	0.0	3.008418493733492	0.0031032521431428217
68-69	0.0	2.170106393806169E-5	0.0	3.385398525933531	0.003157504802987976
70-71	0.0	2.170106393806169E-5	0.0	3.795244819467764	0.0033419638464615006
72-73	0.0	2.170106393806169E-5	0.0	4.214737235922465	0.0034070670382756857
74-75	0.0	2.170106393806169E-5	0.0	4.635672773129048	0.0034179175702447164
76-77	0.0	2.170106393806169E-5	0.0	5.102386704712972	0.0036132271456872714
78-79	0.0	2.170106393806169E-5	0.0	5.617776122709968	0.003862789380974981
80-81	0.0	2.170106393806169E-5	0.0	6.173312508992378	0.003862789380974981
82-83	0.0	2.170106393806169E-5	0.0	6.742553117151674	0.003960444168696259
84-85	0.0	2.170106393806169E-5	0.0	7.310545914134533	0.004058098956417537
86-87	0.0	2.170106393806169E-5	0.0	7.902865603791957	0.004101501084293659
88	0.0	2.170106393806169E-5	0.0	8.375503925830971	0.0041232021482317215
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6775	0.0	60.1204	1
GTATCAA	13390	0.0	43.7674	1
CAACGCA	16470	0.0	34.986	5
ATCAACG	16625	0.0	34.65523	3
TATCAAC	17340	0.0	33.76946	2
AACGCAG	17765	0.0	33.01555	6
TCAACGC	17965	0.0	32.305588	4
ACGCAGA	19205	0.0	29.977188	7
CGCAGAG	19625	0.0	29.287745	8
TGATCGC	1455	0.0	26.163216	9
GTGATCG	1440	0.0	26.109383	8
GCAGAGT	22205	0.0	25.694315	9
GAGTACT	14820	0.0	23.594093	12-13
GTGGTAT	2185	0.0	23.463257	1
GTACATG	12510	0.0	22.59594	1
CAGAGTA	21370	0.0	22.432077	10-11
AGAGTAC	20215	0.0	22.22583	10-11
TACATGG	12535	0.0	21.799955	2
ACATGGG	12330	0.0	21.208313	3
GTATAAG	2725	0.0	21.05749	1
>>END_MODULE