FastQCFastQC Report
Mon 16 Jan 2017
ERR1146632_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146632_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3956375
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA59900.036.5874981
CAACGCA60500.033.643625
ATCAACG61050.033.2652133
GGTATCA36900.033.1375661
AACGCAG69950.030.4332856
TATCAAC73550.029.4009842
GTACATG128550.027.2923131
TCAACGC77100.026.7668254
ACGCAGA77200.026.6012787
CGCAGAG77650.026.2652268
TACATGG129950.026.2269922
ACATGGG129450.025.493373
GTACTAG15550.024.1954251
CATGGGG102650.023.1727894
CTAACGC6150.021.4030253
ATGGGGG68400.020.8236475
TAACGCC6550.020.813424
GCAGAGT96400.020.4245919
CTAGTAC23550.020.1614933
CTGTGCG23500.019.5963259
Produced by FastQC (version 0.11.5)