FastQCFastQC Report
Mon 16 Jan 2017
ERR1146632_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146632_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3956375
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103130.2606679093867493No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90920.22980632523458977No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51890.13115541373100376No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70050.056.7294431
GTATCAA131900.044.033391
ATCAACG160300.035.3203853
CAACGCA161400.035.1192865
TATCAAC169850.033.9725382
AACGCAG175000.032.9259156
TCAACGC174900.032.479524
GTGGTAT20500.031.887531
ACGCAGA190700.029.6722347
CGCAGAG192350.029.2711038
GTACTAG17150.027.4217911
GCAGAGT215850.025.9101149
GTACATG127850.024.6452921
TACATGG127200.024.2530142
ACATGGG129050.023.284953
GAGTACT143050.022.78473712-13
CAGAGTA207200.022.44429610-11
AGAGTAC197250.022.32556710-11
TGGTATC27500.021.5472
CATGGGG99800.021.2509574
Produced by FastQC (version 0.11.5)