##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146632_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3956375
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.361706359988624	33.0	31.0	34.0	30.0	34.0
2	32.45198445546744	34.0	31.0	34.0	30.0	34.0
3	32.551083251714005	34.0	31.0	34.0	30.0	34.0
4	36.03807904963508	37.0	35.0	37.0	35.0	37.0
5	36.081599696692045	37.0	35.0	37.0	35.0	37.0
6	36.14467106884459	37.0	36.0	37.0	35.0	37.0
7	36.10772335787179	37.0	36.0	37.0	35.0	37.0
8	36.11597485071562	37.0	36.0	37.0	35.0	37.0
9	37.91060579444567	39.0	38.0	39.0	35.0	39.0
10-11	37.85606596947964	39.0	38.0	39.0	35.0	39.0
12-13	37.826437079397174	39.0	38.0	39.0	35.0	39.0
14-15	39.18405206786515	40.0	39.0	41.0	36.0	41.0
16-17	39.13473015070614	40.0	39.0	41.0	36.0	41.0
18-19	39.23243815361284	40.0	39.0	41.0	36.0	41.0
20-21	39.28494429875833	41.0	39.0	41.0	36.0	41.0
22-23	39.2503350920982	40.5	39.0	41.0	36.0	41.0
24-25	39.23616226975451	41.0	39.0	41.0	36.0	41.0
26-27	39.16148380777858	41.0	39.0	41.0	35.5	41.0
28-29	39.10430482449212	40.0	39.0	41.0	35.0	41.0
30-31	38.96854961928533	40.0	38.5	41.0	35.0	41.0
32-33	38.8655093361979	40.0	38.0	41.0	35.0	41.0
34-35	38.794151717165335	40.0	38.0	41.0	35.0	41.0
36-37	38.727513317114784	40.0	38.0	41.0	35.0	41.0
38-39	38.640004549619285	40.0	38.0	41.0	35.0	41.0
40-41	38.54103946162839	40.0	38.0	41.0	35.0	41.0
42-43	38.43627879055954	40.0	38.0	41.0	34.0	41.0
44-45	38.28671966130612	40.0	38.0	41.0	34.0	41.0
46-47	38.049686392215094	40.0	38.0	41.0	33.5	41.0
48-49	37.77307017155856	40.0	37.0	41.0	33.0	41.0
50-51	37.66009351995198	40.0	37.0	41.0	33.0	41.0
52-53	37.55040434741398	40.0	37.0	41.0	33.0	41.0
54-55	37.383464914220724	40.0	36.5	41.0	32.5	41.0
56-57	37.22228327698967	39.5	36.0	41.0	32.0	41.0
58-59	37.031248428169725	39.0	35.5	41.0	32.0	41.0
60-61	36.82333196423494	39.0	35.0	41.0	32.0	41.0
62-63	36.583065432371804	39.0	35.0	41.0	31.5	41.0
64-65	36.30722631196487	38.0	35.0	40.5	31.0	41.0
66-67	36.03374743294051	37.0	35.0	40.0	31.0	41.0
68-69	35.739975735363814	37.0	35.0	40.0	31.0	41.0
70-71	35.44035221635967	36.0	35.0	39.0	31.0	41.0
72-73	35.12347805756532	36.0	35.0	39.0	30.0	41.0
74-75	34.81120394300338	35.0	35.0	38.5	30.0	40.5
76-77	34.521764999526084	35.0	34.5	37.0	30.0	39.5
78-79	34.24289684370162	35.0	34.0	37.0	30.0	39.0
80-81	33.993915768854066	35.0	34.0	36.5	30.0	39.0
82-83	33.74897475593188	35.0	34.0	36.0	29.0	37.5
84-85	33.546245237117304	35.0	34.0	36.0	29.0	37.0
86-87	33.36746782092193	35.0	34.0	35.0	29.0	37.0
88-89	33.18111503585985	35.0	34.0	35.0	29.0	36.0
90-91	33.10022988215222	35.0	34.0	35.0	29.0	36.0
92-93	33.00357486335345	35.0	34.0	35.0	29.0	36.0
94-95	32.93578262930082	35.0	34.0	35.0	29.0	36.0
96-97	32.88027765315472	35.0	34.0	35.0	29.0	35.0
98-99	32.81479561467252	35.0	34.0	35.0	29.0	35.0
100	32.08298429749455	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3563419118049467
1101	2	0.3612440259573759
1101	3	0.31578221031670495
1101	4	0.2048516126970057
1101	5	0.20785228275600787
1101	6	0.25918899194146405
1101	7	0.27068920337875824
1101	8	0.26262041507199285
1101	9	0.3112283353058345
1101	10-11	0.3140901669547276
1101	12-13	0.35883307998159353
1101	14-15	0.48735982871734507
1101	16-17	0.5497862690580391
1101	18-19	0.4886013404679659
1101	20-21	0.4435384646350258
1101	22-23	0.42238789047987524
1101	24-25	0.4302559756656237
1101	26-27	0.4542262516115372
1101	28-29	0.4781536593837856
1101	30-31	0.5201297715322895
1101	32-33	0.48383654934391984
1101	34-35	0.47262625631232424
1101	36-37	0.4951993219046358
1101	38-39	0.5379886950379458
1101	40-41	0.5711014019050396
1101	42-43	0.5926236509503369
1101	44-45	0.5904616422103643
1101	46-47	0.6123053159352807
1101	48-49	0.7012227312675421
1101	50-51	0.6247927626713334
1101	52-53	0.5907057001466782
1101	54-55	0.5551936752754898
1101	56-57	0.5396872961625263
1101	58-59	0.5443467783856519
1101	60-61	0.48312737685706253
1101	62-63	0.506860995245006
1101	64-65	0.4964161924270982
1101	66-67	0.43811285412175494
1101	68-69	0.4294838860566088
1101	70-71	0.41705340078552666
1101	72-73	0.3879160501254333
1101	74-75	0.37081346251889613
1101	76-77	0.3467580217163544
1101	78-79	0.29952597620115995
1101	80-81	0.3239585602067976
1101	82-83	0.3540338187149743
1101	84-85	0.3777895746584079
1101	86-87	0.39706935938940546
1101	88-89	0.3862146209568351
1101	90-91	0.3615640010712653
1101	92-93	0.37302612794687207
1101	94-95	0.3776007858899746
1101	96-97	0.4134066922010362
1101	98-99	0.41773225872505293
1101	100	0.4388902498317009
1102	1	0.25574948733499525
1102	2	0.2388735572454337
1102	3	0.26848249486840814
1102	4	0.1832518328140793
1102	5	0.15899227333857624
1102	6	0.19132299243428008
1102	7	0.19415420846722498
1102	8	0.19591948836130513
1102	9	0.2762190555277826
1102	10-11	0.2668931222738493
1102	12-13	0.28390121980752525
1102	14-15	0.40748251849309014
1102	16-17	0.4433117995538467
1102	18-19	0.36936682731143833
1102	20-21	0.3793924467032994
1102	22-23	0.3959000392694847
1102	24-25	0.3368628178642936
1102	26-27	0.3411975782400347
1102	28-29	0.39453030863080585
1102	30-31	0.40570996111625846
1102	32-33	0.4241976366818392
1102	34-35	0.3926829878980982
1102	36-37	0.4493593415269146
1102	38-39	0.42805771845472407
1102	40-41	0.4524595508037095
1102	42-43	0.4959000382426524
1102	44-45	0.5252292185972252
1102	46-47	0.5512961963575691
1102	48-49	0.6649103152661837
1102	50-51	0.5861490697186653
1102	52-53	0.5398275926165041
1102	54-55	0.5258323813515347
1102	56-57	0.48079276070617283
1102	58-59	0.49771320671534625
1102	60-61	0.44202835351344305
1102	62-63	0.4451509614769833
1102	64-65	0.48659279670639677
1102	66-67	0.43128491810927017
1102	68-69	0.426570223554819
1102	70-71	0.36307014835814044
1102	72-73	0.3705235655577255
1102	74-75	0.37109747116880953
1102	76-77	0.3796540750030317
1102	78-79	0.40494933840348324
1102	80-81	0.3681462671840805
1102	82-83	0.35131750281589547
1102	84-85	0.3180073188973651
1102	86-87	0.32822720506895564
1102	88-89	0.4039438920400187
1102	90-91	0.32531273498702973
1102	92-93	0.31035460931177283
1102	94-95	0.36588953025496096
1102	96-97	0.3594399387491336
1102	98-99	0.3685128733377212
1102	100	0.35107131332210173
1103	1	0.22650909051902346
1103	2	0.2288380804013599
1103	3	0.24457170832920383
1103	4	0.18079835779284537
1103	5	0.17274099392892595
1103	6	0.164029750881042
1103	7	0.1910075545310832
1103	8	0.21775737372715298
1103	9	0.2520866555688315
1103	10-11	0.23895591868178911
1103	12-13	0.19608705803911164
1103	14-15	0.3226803378903611
1103	16-17	0.4322768076930501
1103	18-19	0.35993252956807
1103	20-21	0.31038474067861443
1103	22-23	0.35708458627754425
1103	24-25	0.2986237247364727
1103	26-27	0.34317474021642624
1103	28-29	0.34966372779152266
1103	30-31	0.3444500912079178
1103	32-33	0.245661026529838
1103	34-35	0.30621309757309234
1103	36-37	0.3379827423862878
1103	38-39	0.4004355945212481
1103	40-41	0.36692419455473413
1103	42-43	0.31811548395379674
1103	44-45	0.33693905173313254
1103	46-47	0.3810399544203875
1103	48-49	0.3954172153622295
1103	50-51	0.36589310090306526
1103	52-53	0.3353248551866912
1103	54-55	0.366683206044911
1103	56-57	0.36923553471374504
1103	58-59	0.38554253864638355
1103	60-61	0.39458731762229604
1103	62-63	0.3739368546481643
1103	64-65	0.36547206263183085
1103	66-67	0.32802301786273347
1103	68-69	0.3498443765122232
1103	70-71	0.2769819866903944
1103	72-73	0.31762103282611776
1103	74-75	0.27326500805462217
1103	76-77	0.2603127372153864
1103	78-79	0.23393972082099168
1103	80-81	0.2361818180092783
1103	82-83	0.21788228474444082
1103	84-85	0.23375884189847085
1103	86-87	0.22485237179684958
1103	88-89	0.2811687927071631
1103	90-91	0.2502377887745624
1103	92-93	0.27041844130879156
1103	94-95	0.21359546842045063
1103	96-97	0.15730074785111015
1103	98-99	0.20439152875393063
1103	100	0.1598805952326643
1104	1	0.1750205294415892
1104	2	0.17963928469247747
1104	3	0.18423347814933777
1104	4	0.16694094871367327
1104	5	0.0739804753720179
1104	6	0.13187788576798454
1104	7	0.14452542434341353
1104	8	0.1315456251193936
1104	9	0.21593377281289605
1104	10-11	0.19250642954437325
1104	12-13	0.15917345795175208
1104	14-15	0.3417173046118194
1104	16-17	0.33317146529187625
1104	18-19	0.3176320021265937
1104	20-21	0.2694353632442059
1104	22-23	0.24748999501152724
1104	24-25	0.25975703578087916
1104	26-27	0.2316885699452058
1104	28-29	0.23409243103925093
1104	30-31	0.26422815618100515
1104	32-33	0.24863611615492687
1104	34-35	0.2794744449026325
1104	36-37	0.24917789917788014
1104	38-39	0.21909080736586617
1104	40-41	0.2368895709267136
1104	42-43	0.1863522798738515
1104	44-45	0.15752432385011872
1104	46-47	0.252199545385821
1104	48-49	0.3604882944787917
1104	50-51	0.2817021519648577
1104	52-53	0.2728923442183415
1104	54-55	0.2874340604394803
1104	56-57	0.25046016146463046
1104	58-59	0.2752790383855128
1104	60-61	0.24872774441565326
1104	62-63	0.17513101756713922
1104	64-65	0.18714444535788033
1104	66-67	0.20158464562970835
1104	68-69	0.12944936985224587
1104	70-71	0.12173856799598326
1104	72-73	0.14942442243124532
1104	74-75	0.11642893003096333
1104	76-77	0.15178987478647343
1104	78-79	0.06848867920977142
1104	80-81	0.15640473140646094
1104	82-83	0.12600506607886786
1104	84-85	0.14811010403918345
1104	86-87	0.13897070258005328
1104	88-89	0.20836440285624036
1104	90-91	0.16634288296595656
1104	92-93	0.14808087015086357
1104	94-95	0.1625807796033456
1104	96-97	0.1712035271288812
1104	98-99	0.15384487298024396
1104	100	0.21182816354331635
1105	1	0.11088339503680089
1105	2	0.08489282614838345
1105	3	0.11750944905339367
1105	4	0.0863805367185364
1105	5	0.0422217198574657
1105	6	0.042434867894307615
1105	7	0.09512635758230914
1105	8	0.06117742862210207
1105	9	0.08905953889584595
1105	10-11	0.1075274092028593
1105	12-13	0.11315476203698438
1105	14-15	0.19224363780278964
1105	16-17	0.2635960708529552
1105	18-19	0.18969892260676602
1105	20-21	0.12228601436556374
1105	22-23	0.17187546347431493
1105	24-25	0.20148292265555057
1105	26-27	0.15500537025156547
1105	28-29	0.13145096660564093
1105	30-31	0.19784581392246992
1105	32-33	0.1836183063564789
1105	34-35	0.11302848074436156
1105	36-37	0.0836898586986905
1105	38-39	0.09226233178285526
1105	40-41	0.11368136440783871
1105	42-43	0.09165093766684151
1105	44-45	0.08762704818030898
1105	46-47	0.15096941499142957
1105	48-49	0.2153184469578946
1105	50-51	0.1642545200589609
1105	52-53	0.10821659040556852
1105	54-55	0.09409339871072575
1105	56-57	0.06759034191874491
1105	58-59	0.09685209467147615
1105	60-61	0.1424946921325514
1105	62-63	0.1099825132355079
1105	64-65	0.08276271973743121
1105	66-67	0.07592177974559888
1105	68-69	0.017917386927436496
1105	70-71	0.011677255680346832
1105	72-73	0.03310855093399567
1105	74-75	0.054723375200332214
1105	76-77	-0.01538289414165206
1105	78-79	-0.008243071504729471
1105	80-81	0.02228411718869694
1105	82-83	0.04883385238840532
1105	84-85	0.043803893312336584
1105	86-87	0.04038763804282297
1105	88-89	0.12448807651055915
1105	90-91	0.11670712797189964
1105	92-93	0.09758284902628134
1105	94-95	0.07253267958535758
1105	96-97	0.0959416926771155
1105	98-99	0.10318479577047412
1105	100	0.05982860780522259
1106	1	0.11738865990558622
1106	2	0.08739557249702301
1106	3	0.1120342167410655
1106	4	0.0904835402201698
1106	5	0.07597031424383971
1106	6	0.08410790839796789
1106	7	0.11688261674660794
1106	8	0.11495684926714489
1106	9	0.11914304505176432
1106	10-11	0.10337025819438139
1106	12-13	0.1553797319829684
1106	14-15	0.2094349638826145
1106	16-17	0.2585238546520756
1106	18-19	0.2583039982729076
1106	20-21	0.2095929655125488
1106	22-23	0.22377752456333866
1106	24-25	0.18514654911856354
1106	26-27	0.1561223739739077
1106	28-29	0.21812562799846802
1106	30-31	0.2569638835604664
1106	32-33	0.1880133404920059
1106	34-35	0.1713908528946746
1106	36-37	0.19445456447432719
1106	38-39	0.15206339488813114
1106	40-41	0.08344087352948293
1106	42-43	0.15225837122476094
1106	44-45	0.14388899825987522
1106	46-47	0.14558555083162616
1106	48-49	0.23672986552632835
1106	50-51	0.1351412720396752
1106	52-53	0.11223955645102279
1106	54-55	0.18286365471343657
1106	56-57	0.1352829659859438
1106	58-59	0.147152591810773
1106	60-61	0.17526329659017392
1106	62-63	0.18753676351807513
1106	64-65	0.15450237028399982
1106	66-67	0.11103087252026711
1106	68-69	0.09983753746550406
1106	70-71	0.15827423554088682
1106	72-73	0.17188218462190008
1106	74-75	0.1892343916966297
1106	76-77	0.07380290784703902
1106	78-79	0.08061985944723205
1106	80-81	0.0754090786574082
1106	82-83	0.10208374362448325
1106	84-85	0.08793069834838008
1106	86-87	0.15390527255748054
1106	88-89	0.1838244580728201
1106	90-91	0.10982206185121868
1106	92-93	0.1362622488332903
1106	94-95	0.1112893187661399
1106	96-97	0.06332180168733004
1106	98-99	0.0668295570084183
1106	100	0.05741666076818319
1107	1	0.06317899662474247
1107	2	0.031212810545291347
1107	3	0.08782315663642493
1107	4	0.052737432131927164
1107	5	0.03373445766169425
1107	6	0.10098219665322006
1107	7	0.08486097127397585
1107	8	0.059947762515626835
1107	9	0.10642972948057405
1107	10-11	0.10476845780360833
1107	12-13	0.11166315637711932
1107	14-15	0.19677919591312332
1107	16-17	0.25140097052131694
1107	18-19	0.16389681742103335
1107	20-21	0.16704528673278674
1107	22-23	0.1490523779745061
1107	24-25	0.1673620619577889
1107	26-27	0.1942962131182142
1107	28-29	0.15121507587790006
1107	30-31	0.18347140955889785
1107	32-33	0.18195334133288554
1107	34-35	0.1619353972977038
1107	36-37	0.13345987622152222
1107	38-39	0.1797305065402739
1107	40-41	0.200380351826297
1107	42-43	0.22579251915795595
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2216	32-33	-0.5934815252427228
2216	34-35	-0.5695895607274082
2216	36-37	-0.5900187973833511
2216	38-39	-0.6132913136439342
2216	40-41	-0.5707005789069726
2216	42-43	-0.5571563306006695
2216	44-45	-0.5745695582919481
2216	46-47	-0.5858187522484712
2216	48-49	-0.4710237565765354
2216	50-51	-0.5383786295041233
2216	52-53	-0.5259196328524709
2216	54-55	-0.49600429481853325
2216	56-57	-0.531818441370838
2216	58-59	-0.5522116834748019
2216	60-61	-0.5201997206822995
2216	62-63	-0.5893397525334905
2216	64-65	-0.5001035110453884
2216	66-67	-0.4775230289105963
2216	68-69	-0.43802475060417123
2216	70-71	-0.5031466958520596
2216	72-73	-0.48853029663811043
2216	74-75	-0.447508971094436
2216	76-77	-0.4947378453475437
2216	78-79	-0.4400368033437232
2216	80-81	-0.47004112455831404
2216	82-83	-0.4498871090057932
2216	84-85	-0.4267327540488921
2216	86-87	-0.4038535811836539
2216	88-89	-0.3994904070396359
2216	90-91	-0.4286540794583331
2216	92-93	-0.42898946326066323
2216	94-95	-0.4140465489540759
2216	96-97	-0.4445251602173528
2216	98-99	-0.4710692027245642
2216	100	-0.5512761786862015
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	26.0
8	136.0
9	266.0
10	254.0
11	181.0
12	190.0
13	274.0
14	499.0
15	907.0
16	1782.0
17	3078.0
18	5199.0
19	7978.0
20	11397.0
21	17016.0
22	24408.0
23	27908.0
24	26012.0
25	22703.0
26	21969.0
27	25302.0
28	31448.0
29	39221.0
30	48444.0
31	60782.0
32	77941.0
33	102022.0
34	148111.0
35	230159.0
36	412154.0
37	948102.0
38	1237165.0
39	422520.0
40	820.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.17444406305497	20.068528779711517	13.397335991713458	24.359691165520054
2	15.332240546275571	20.9989017363687	39.77918455505157	23.889673162304152
3	17.62116163107173	26.51040044808774	30.91540018522038	24.953037735620146
4	12.299415340730926	17.854285309677223	39.14582090005159	30.700478449540263
5	12.695566102524323	38.4365985039953	35.39904116780676	13.46879422567362
6	29.421529007216034	38.852450894649095	18.147772552770494	13.578247545364375
7	25.644965403936688	33.53291839120407	23.303048876812742	17.519067328046507
8	25.44914425063259	35.037232484328385	20.872939423818426	18.6406838412206
9	26.281148486542723	14.813874933904092	20.978714842790314	37.926261736762875
10-11	24.34180245391423	26.197251876908474	28.986953454590857	20.47399221458644
12-13	25.375880698872074	24.197491390477392	28.62267858835424	21.803949322296294
14-15	22.661924782641883	25.114220748594544	26.695782552407838	25.52807191635573
16-17	21.563665724850612	27.981149861482645	27.48234584612865	22.972838567538094
18-19	21.442309673047475	27.116362614765887	29.90053128253061	21.540796429656027
20-21	22.66919541917839	26.262898678824964	29.41591958981535	21.651986312181297
22-23	22.607487915073772	26.07987109412025	29.221307383652967	22.091333607153015
24-25	22.260339325771696	26.265217528672082	29.572057754889258	21.90238539066696
26-27	22.154688263603685	26.479594203111013	29.168136345409113	22.19758118787619
28-29	21.971555018363404	26.657853041970625	29.32792383531943	22.042668104346543
30-31	22.40571119702323	26.347886957445947	29.164838917305097	22.081562928225722
32-33	22.289764537798877	26.711042389262374	28.780857693326013	22.21833537961274
34-35	22.50107358540116	26.52526849745309	29.146990586570666	21.82666733057508
36-37	22.429248311331694	26.31518219217	29.10303935019255	22.152530146305757
38-39	22.489256175369043	26.360306267720755	29.138832141049463	22.011605415860735
40-41	22.45585770531598	26.028996906999957	29.24399061458874	22.271154773095322
42-43	22.429787368487567	26.137411140248336	28.928488831316546	22.504312659947555
44-45	22.467936699791867	26.549826753347496	28.68807097242448	22.294165574436157
46-47	22.57154592271966	26.452826135035227	28.1569871410066	22.81864080123851
48-49	22.922357382560936	26.451386889652156	28.097946325053947	22.52830940273296
50-51	22.6664307604815	26.72503238444283	27.71002495971691	22.898511895358755
52-53	22.49228805110151	26.84429287978181	27.719554373268057	22.94386469584862
54-55	22.550679134012512	26.742750031468304	27.57734251820233	23.12922831631686
56-57	22.709036313590488	26.65181719606609	27.589406450862786	23.049740039480636
58-59	22.360964019866795	26.631731267456114	28.088073603194864	22.919231109482226
60-61	22.495420334032843	26.87868039902966	27.740687591269474	22.885211675668025
62-63	22.576188890440537	26.632131620735432	27.609644452897765	23.182035035926262
64-65	22.123142690575673	26.708782804604684	27.740690530933538	23.42738397388611
66-67	22.447202022176864	26.57740550239673	27.672106766611133	23.303285708815277
68-69	22.205198609447613	26.36086848987411	27.90808361260526	23.52584928807302
70-71	22.260851915544247	26.19428543696511	27.771255708250198	23.773606939240448
72-73	22.457673922950754	26.29459116450962	27.80431666459821	23.443418247941423
74-75	22.352978198893048	26.643436856005927	27.54319918246165	23.460385762639376
76-77	22.314531621252716	26.58082250644558	27.386646276730197	23.717999595571506
78-79	22.174918203731316	26.711731232291797	27.289211814959014	23.82413874901787
80-81	22.07563296342406	26.69148848914417	27.511061070352945	23.721817477078826
82-83	22.365109417263987	26.333733997700655	27.792466557837276	23.508690027198078
84-85	22.463235400982242	26.593920386537757	27.16373802290417	23.77910618957583
86-87	21.910701083694036	26.708148241761716	27.453439069855612	23.927711604688636
88-89	22.20186408012385	26.40176929638874	27.22713342390446	24.16923319958295
90-91	22.09426558402578	26.35440270449591	27.333379672048274	24.217952039430035
92-93	22.20790916526555	26.42367805656293	27.12873056426443	24.239682213907084
94-95	21.711762912052116	26.384693651746304	27.410671621711963	24.492871814489614
96-97	21.840050379584593	26.254216465646678	27.362165442542008	24.543567712226714
98-99	21.680231494944294	26.60418770077582	27.113694960142144	24.60188584413774
100	21.96068729940951	26.219655945572946	26.830736027974407	24.98892072704314
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	138.0
1	90.5
2	165.0
3	284.0
4	192.0
5	160.5
6	344.0
7	1000.5
8	1904.0
9	2250.0
10	1770.5
11	1289.0
12	1331.0
13	1587.5
14	1879.5
15	2219.0
16	2700.5
17	3158.5
18	3670.0
19	4278.5
20	5010.5
21	5994.5
22	7187.0
23	8749.5
24	10791.0
25	13614.5
26	17858.5
27	22952.5
28	27932.0
29	35475.5
30	44516.0
31	52805.0
32	62491.0
33	71875.0
34	79655.0
35	86907.5
36	96454.5
37	109090.0
38	115353.0
39	114583.0
40	114225.5
41	115907.0
42	119250.5
43	125052.0
44	132081.5
45	143986.0
46	154559.5
47	162344.5
48	167958.5
49	164608.5
50	158210.5
51	155359.5
52	152586.0
53	148280.0
54	143418.5
55	129752.0
56	116336.5
57	103111.0
58	90054.5
59	81677.5
60	67556.5
61	51775.5
62	39860.0
63	29927.5
64	21366.0
65	14320.5
66	9740.0
67	7003.5
68	5063.5
69	3381.5
70	2283.5
71	1664.5
72	1194.5
73	932.5
74	715.0
75	470.0
76	299.5
77	212.5
78	124.5
79	37.0
80	13.5
81	6.0
82	7.5
83	8.5
84	5.0
85	3.5
86	2.0
87	0.5
88	0.0
89	1.0
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.002047328678398787
2	0.0032858361505165717
3	1.7692963887396923E-4
4	0.048908407317304343
5	0.002628668920413257
6	0.004726548924204607
7	0.0
8	4.5496192853306373E-4
9	7.582698808884396E-5
10-11	5.055132539256264E-5
12-13	0.0
14-15	2.527566269628132E-5
16-17	2.9067012100723514E-4
18-19	1.3901614482954725E-4
20-21	2.6539445831095387E-4
22-23	0.0
24-25	0.0
26-27	2.9067012100723514E-4
28-29	1.263783134814066E-5
30-31	1.6429180752582857E-4
32-33	1.895674702221099E-4
34-35	1.516539761776879E-4
36-37	7.582698808884396E-5
38-39	8.214590376291429E-4
40-41	5.56064579318189E-4
42-43	0.0
44-45	5.434267479700484E-4
46-47	0.0
48-49	1.0110265078512527E-4
50-51	0.0
52-53	3.664971090960791E-4
54-55	3.2858361505165714E-4
56-57	1.263783134814066E-4
58-59	6.318915674070329E-4
60-61	6.950807241477362E-4
62-63	1.895674702221099E-4
64-65	3.412214463997978E-4
66-67	0.0011247669899845185
68-69	0.002666582414457679
70-71	0.003778711573094057
72-73	0.0031847334997314462
74-75	0.002729771571198382
76-77	0.004423240971849231
78-79	0.0013016966288584878
80-81	0.0011247669899845185
82-83	7.456320495402989E-4
84-85	5.434267479700484E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	7.582698808884396E-5
94-95	8.846481943698462E-5
96-97	2.6539445831095387E-4
98-99	8.846481943698462E-5
100	0.07701494423556918
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3956375.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.691391577979694
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.2529326017412	32.25947484500878
2	15.864764908406439	15.44954960895618
3	6.992964176692047	10.214914710542905
4	3.5997143439152843	7.011004027537974
5	2.130572971577166	5.187028142226181
6	1.3684696810982662	3.99796158629692
7	0.8925097744343078	3.0420280039908643
8	0.619809244927182	2.4143499718721078
9	0.44451537894342935	1.9479665140711693
>10	1.7714606118502845	13.637056908881931
>50	0.04312686509986659	1.4020798215668426
>100	0.017115316062456337	1.554901332707267
>500	0.0013008069786289219	0.43002047083270084
>1k	4.645739209387756E-4	0.3389945700103907
>5k	1.8582956837551024E-4	0.6460938815318817
>10k+	9.291478418775512E-5	0.4665756039659895
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10313	0.2606679093867493	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9092	0.22980632523458977	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5189	0.13115541373100376	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.527566269628132E-5	0.0
5	0.0	0.0	0.0	2.527566269628132E-5	0.0
6	0.0	0.0	0.0	1.0110265078512527E-4	0.0
7	0.0	0.0	0.0	1.0110265078512527E-4	0.0
8	0.0	0.0	0.0	1.0110265078512527E-4	0.0
9	0.0	0.0	0.0	1.263783134814066E-4	0.0
10-11	0.0	0.0	0.0	1.516539761776879E-4	0.0
12-13	0.0	0.0	0.0	1.516539761776879E-4	0.0
14-15	0.0	0.0	0.0	1.895674702221099E-4	0.0
16-17	0.0	0.0	0.0	5.813402420144704E-4	0.0
18-19	0.0	0.0	0.0	8.846481943698461E-4	0.0
20-21	0.0	0.0	0.0	9.099238570661275E-4	0.0
22-23	0.0	0.0	0.0	0.001074215664591956	0.0
24-25	0.0	0.0	0.0	0.0014912640990805979	0.0
26-27	0.0	0.0	0.0	0.002097880003791349	0.0
28-29	0.0	0.0	0.0	0.005004581213863701	0.0
30-31	0.0	0.0	0.0	0.015215948943161353	0.0
32-33	0.0	0.0	0.0	0.038671763925310416	0.0
34-35	0.0	0.0	0.0	0.07855675966004233	0.0
36-37	0.0	0.0	0.0	0.1403683927837983	0.0
38-39	0.0	0.0	0.0	0.23502574958137182	0.0
40-41	0.0	0.0	0.0	0.34201762977473066	0.0
42-43	0.0	0.0	0.0	0.4545827935926195	0.0
44-45	0.0	0.0	0.0	0.59262582540836	0.0
46-47	0.0	0.0	0.0	0.7625541057154592	0.0
48-49	0.0	0.0	0.0	0.9495813718365929	0.0
50-51	0.0	0.0	0.0	1.1514075384663993	0.0
52-53	1.263783134814066E-5	0.0	0.0	1.3543458342548418	0.0
54-55	2.527566269628132E-5	0.0	0.0	1.5796404536981454	0.0
56-57	2.527566269628132E-5	0.0	0.0	1.8461596789990837	0.0
58-59	2.527566269628132E-5	0.0	0.0	2.1447916337556476	0.0
60-61	2.527566269628132E-5	0.0	0.0	2.464490853369562	2.527566269628132E-5
62-63	2.527566269628132E-5	0.0	0.0	2.791848598780449	2.527566269628132E-5
64-65	2.527566269628132E-5	0.0	0.0	3.133461817952039	2.527566269628132E-5
66-67	2.527566269628132E-5	0.0	0.0	3.521809737449054	2.527566269628132E-5
68-69	2.527566269628132E-5	0.0	0.0	3.956626962813181	2.527566269628132E-5
70-71	2.527566269628132E-5	0.0	0.0	4.427234526555242	2.527566269628132E-5
72-73	2.527566269628132E-5	0.0	0.0	4.905273135130011	2.527566269628132E-5
74-75	2.527566269628132E-5	0.0	0.0	5.3853085210577865	2.527566269628132E-5
76-77	2.527566269628132E-5	0.0	0.0	5.916817794066538	2.527566269628132E-5
78-79	2.527566269628132E-5	0.0	0.0	6.5002559160848	2.527566269628132E-5
80-81	2.527566269628132E-5	0.0	0.0	7.1303781871030925	2.527566269628132E-5
82-83	2.527566269628132E-5	0.0	0.0	7.777321411645762	2.527566269628132E-5
84-85	2.527566269628132E-5	0.0	0.0	8.413257085084199	2.527566269628132E-5
86-87	2.527566269628132E-5	0.0	0.0	9.08165934725601	2.527566269628132E-5
88	2.527566269628132E-5	0.0	0.0	9.61013554074121	2.527566269628132E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7005	0.0	56.729443	1
GTATCAA	13190	0.0	44.03339	1
ATCAACG	16030	0.0	35.320385	3
CAACGCA	16140	0.0	35.119286	5
TATCAAC	16985	0.0	33.972538	2
AACGCAG	17500	0.0	32.925915	6
TCAACGC	17490	0.0	32.47952	4
GTGGTAT	2050	0.0	31.88753	1
ACGCAGA	19070	0.0	29.672234	7
CGCAGAG	19235	0.0	29.271103	8
GTACTAG	1715	0.0	27.421791	1
GCAGAGT	21585	0.0	25.910114	9
GTACATG	12785	0.0	24.645292	1
TACATGG	12720	0.0	24.253014	2
ACATGGG	12905	0.0	23.28495	3
GAGTACT	14305	0.0	22.784737	12-13
CAGAGTA	20720	0.0	22.444296	10-11
AGAGTAC	19725	0.0	22.325567	10-11
TGGTATC	2750	0.0	21.547	2
CATGGGG	9980	0.0	21.250957	4
>>END_MODULE