FastQCFastQC Report
Mon 16 Jan 2017
ERR1146631_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146631_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4361781
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39250.037.503171
GTATCAA62700.036.5278971
CAACGCA67650.031.759385
ATCAACG68800.031.2295953
AACGCAG76500.028.8729136
TATCAAC79800.028.6333392
TCAACGC77200.028.0139374
ACGCAGA82750.025.89627
CGCAGAG85550.024.9922688
GTACATG144250.024.5494861
ACATGGG137100.024.2106173
TACATGG142150.024.1773742
GTACTAG19550.023.8150621
CATGGGG98800.021.4135744
GCATCGT18600.020.4638048
GCAGAGT104700.019.7469659
GTATAAG26150.019.0632721
CGACGAG9650.018.991624-25
GTCGCCG8950.018.9359494
TAACGCC5250.018.8058784
Produced by FastQC (version 0.11.5)