##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146631_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4361781 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.309614123221685 31.0 31.0 34.0 28.0 34.0 2 31.49329390907063 33.0 31.0 34.0 28.0 34.0 3 31.516216884800038 33.0 31.0 34.0 28.0 34.0 4 35.006753892504 37.0 35.0 37.0 32.0 37.0 5 35.07265357889358 37.0 35.0 37.0 32.0 37.0 6 35.08208000355818 37.0 35.0 37.0 32.0 37.0 7 35.03493320732976 37.0 35.0 37.0 32.0 37.0 8 35.02510602893634 37.0 35.0 37.0 32.0 37.0 9 36.61103251171941 39.0 37.0 39.0 32.0 39.0 10-11 36.61115975332095 39.0 37.0 39.0 32.0 39.0 12-13 36.56861348609662 39.0 37.0 39.0 32.0 39.0 14-15 37.9037981274163 40.0 38.0 41.0 32.0 41.0 16-17 37.919007854818936 40.0 38.0 41.0 32.0 41.0 18-19 37.929564551727836 40.0 38.0 41.0 32.0 41.0 20-21 38.032414969940035 40.0 38.0 41.0 32.0 41.0 22-23 38.032866964205674 40.0 38.0 41.0 32.0 41.0 24-25 38.05197727717187 40.0 38.0 41.0 32.0 41.0 26-27 38.10944611845483 40.0 38.0 41.0 32.0 41.0 28-29 38.08582228222829 40.0 38.0 41.0 32.0 41.0 30-31 38.049384872830615 40.0 38.0 41.0 32.0 41.0 32-33 38.02489877414753 40.0 38.0 41.0 32.0 41.0 34-35 37.9812529560746 40.0 38.0 41.0 32.0 41.0 36-37 37.91535762570381 40.0 38.0 41.0 32.0 41.0 38-39 37.86678113825522 40.0 38.0 41.0 32.0 41.0 40-41 37.823023439278586 40.0 38.0 41.0 31.0 41.0 42-43 37.67740012623284 40.0 37.5 41.0 31.0 41.0 44-45 37.56890935147821 40.0 37.0 41.0 31.0 41.0 46-47 37.471257497797346 40.0 37.0 41.0 31.0 41.0 48-49 37.3807481393495 40.0 37.0 41.0 30.0 41.0 50-51 37.2672002560422 40.0 36.5 41.0 30.0 41.0 52-53 37.1570344774302 40.0 36.0 41.0 30.0 41.0 54-55 37.057778003985064 40.0 36.0 41.0 30.0 41.0 56-57 36.89139722970961 39.0 35.5 41.0 30.0 41.0 58-59 36.71813555059275 39.0 35.0 41.0 30.0 41.0 60-61 36.52221649826069 39.0 35.0 41.0 30.0 41.0 62-63 36.30765735372775 38.5 35.0 40.5 29.5 41.0 64-65 36.03150353032397 38.0 35.0 40.0 29.0 41.0 66-67 35.79877474361963 37.5 35.0 40.0 29.0 41.0 68-69 35.548776864313 37.0 34.5 40.0 29.0 41.0 70-71 35.260725951165355 36.5 34.0 39.0 29.0 41.0 72-73 34.91548715994682 36.0 34.0 39.0 28.5 41.0 74-75 34.57335455402277 35.5 34.0 39.0 28.0 40.0 76-77 34.28288914551189 35.0 34.0 37.5 28.0 39.5 78-79 33.956067257847195 35.0 34.0 37.0 28.0 39.0 80-81 33.692744775585936 35.0 34.0 37.0 28.0 39.0 82-83 33.41218610012745 35.0 34.0 36.0 27.0 38.0 84-85 33.200499864619516 35.0 33.0 36.0 27.0 37.0 86-87 32.98943814464779 35.0 33.0 35.5 27.0 37.0 88-89 32.8128821690039 35.0 33.0 35.0 27.0 36.5 90-91 32.67538535290974 35.0 33.0 35.0 26.5 36.0 92-93 32.53576589471136 35.0 33.0 35.0 26.0 36.0 94-95 32.42694910633982 35.0 33.0 35.0 26.0 36.0 96-97 32.35245958015774 35.0 33.0 35.0 26.0 35.5 98-99 32.26809587643213 35.0 33.0 35.0 25.0 35.0 100 31.16008002235784 34.0 31.0 35.0 23.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.274378209962844 1101 2 0.2768756251919413 1101 3 0.29576605620598784 1101 4 0.34024352582628126 1101 5 0.20156545411609983 1101 6 0.24845785179952884 1101 7 0.302313028498709 1101 8 0.31902263921328 1101 9 0.3842341320049272 1101 10-11 0.3834933302252139 1101 12-13 0.3723084408768145 1101 14-15 0.47265356887058374 1101 16-17 0.5193177422629702 1101 18-19 0.42132614148469116 1101 20-21 0.4542471608430887 1101 22-23 0.5185856133007078 1101 24-25 0.5202490868038154 1101 26-27 0.48033936212196693 1101 28-29 0.470219228335246 1101 30-31 0.4967951836315052 1101 32-33 0.5313636090936882 1101 34-35 0.4719515061630162 1101 36-37 0.42373570134104455 1101 38-39 0.4643696830344908 1101 40-41 0.43528509969996065 1101 42-43 0.4419786818397782 1101 44-45 0.35614562636008174 1101 46-47 0.32122203236146163 1101 48-49 0.3410689979679873 1101 50-51 0.3725220167940009 1101 52-53 0.31960846176097135 1101 54-55 0.3555751396623208 1101 56-57 0.3855933362407882 1101 58-59 0.3172743129884026 1101 60-61 0.3261652420614993 1101 62-63 0.36842561583962663 1101 64-65 0.31121588627669183 1101 66-67 0.2530032946699876 1101 68-69 0.21259499807171522 1101 70-71 0.2285572436356631 1101 72-73 0.22681888307119635 1101 74-75 0.301625855227698 1101 76-77 0.29524456559337864 1101 78-79 0.22211288877711155 1101 80-81 0.2100020953567565 1101 82-83 0.2279643917735612 1101 84-85 0.24186468804351335 1101 86-87 0.2561319596823992 1101 88-89 0.25572402964722585 1101 90-91 0.218232412572263 1101 92-93 0.2316585172770118 1101 94-95 0.2693195690736374 1101 96-97 0.21068872075997547 1101 98-99 0.22876720155030483 1101 100 0.35907521006889453 1102 1 0.2327658351316515 1102 2 0.2502894647085654 1102 3 0.17587864196164205 1102 4 0.18836767834166324 1102 5 0.13376633526424797 1102 6 0.22071453315013656 1102 7 0.2639817318480766 1102 8 0.2328997354377762 1102 9 0.3084173330258082 1102 10-11 0.2869048835184813 1102 12-13 0.32120458773964344 1102 14-15 0.402472993564821 1102 16-17 0.3884432305575416 1102 18-19 0.33893090339827125 1102 20-21 0.3203802577574706 1102 22-23 0.36318028840244665 1102 24-25 0.39252242128066683 1102 26-27 0.40257642555873474 1102 28-29 0.38077284485022034 1102 30-31 0.43401281032620886 1102 32-33 0.3624769041271634 1102 34-35 0.38148701427257237 1102 36-37 0.42128126953219436 1102 38-39 0.41976874291903954 1102 40-41 0.3776278255805252 1102 42-43 0.3848221021654865 1102 44-45 0.3480905238698 1102 46-47 0.30188773881199893 1102 48-49 0.291766956485489 1102 50-51 0.2692303722991838 1102 52-53 0.2885091852634858 1102 54-55 0.17893201227929012 1102 56-57 0.31644554477691855 1102 58-59 0.2954106730210242 1102 60-61 0.2379848945045282 1102 62-63 0.20665424383551567 1102 64-65 0.32501132068551186 1102 66-67 0.24227280769812154 1102 68-69 0.2047998604561272 1102 70-71 0.24731157791226366 1102 72-73 0.26773801823654253 1102 74-75 0.2864005266542833 1102 76-77 0.24285869078484268 1102 78-79 0.2434804640501369 1102 80-81 0.21421909949847873 1102 82-83 0.16201380138249988 1102 84-85 0.1876844790646075 1102 86-87 0.14002820490559031 1102 88-89 0.17009918223246245 1102 90-91 0.16039651742602246 1102 92-93 0.18662276494373486 1102 94-95 0.1595981092073373 1102 96-97 0.14379201895600602 1102 98-99 0.1808057437189987 1102 100 0.1389011041847148 1103 1 0.12626062818062422 1103 2 0.1147167226660315 1103 3 0.1488684755386238 1103 4 0.06650678864654935 1103 5 0.10579121301504557 1103 6 0.14681356114434152 1103 7 0.17984008647624705 1103 8 0.16891123498151472 1103 9 0.17593439467424332 1103 10-11 0.21776999071315117 1103 12-13 0.1923124852817253 1103 14-15 0.2600120130034824 1103 16-17 0.29903928661260437 1103 18-19 0.249246155714701 1103 20-21 0.2173360730568632 1103 22-23 0.20504553022359318 1103 24-25 0.296801106843013 1103 26-27 0.25692827277600827 1103 28-29 0.27510029068729835 1103 30-31 0.3541568075455217 1103 32-33 0.33480351312926615 1103 34-35 0.2635355532235053 1103 36-37 0.27740624631443467 1103 38-39 0.29874774185304886 1103 40-41 0.25061164809876146 1103 42-43 0.2709744914999348 1103 44-45 0.24728593154040368 1103 46-47 0.2436241328750981 1103 48-49 0.20518241816334637 1103 50-51 0.20293438537627395 1103 52-53 0.12115707755592808 1103 54-55 0.12440437395556359 1103 56-57 0.2521014591608619 1103 58-59 0.2880012879891751 1103 60-61 0.22567181652512858 1103 62-63 0.24446277445394315 1103 64-65 0.21218713343309759 1103 66-67 0.18017516858709115 1103 68-69 0.19239157142185803 1103 70-71 0.2113548583319087 1103 72-73 0.223528124943293 1103 74-75 0.16548192269629425 1103 76-77 0.12641739622517179 1103 78-79 0.1720538841374406 1103 80-81 0.19086517366969957 1103 82-83 0.16700253205799243 1103 84-85 0.15167420430312717 1103 86-87 0.11012755233893756 1103 88-89 0.09593287392800676 1103 90-91 0.13141752181120125 1103 92-93 0.18118635809509698 1103 94-95 0.1632840067921748 1103 96-97 0.13756665649680144 1103 98-99 0.07628265932824974 1103 100 0.12362743870282245 1104 1 0.1460243343869685 1104 2 0.14866806682632827 1104 3 0.14598503138475394 1104 4 0.1435464800912385 1104 5 0.17112586068556368 1104 6 0.1954273013109855 1104 7 0.22372860565149466 1104 8 0.279767770357914 1104 9 0.3050033462335833 1104 10-11 0.39995921971780035 1104 12-13 0.355951706583717 1104 14-15 0.3973377287112214 1104 16-17 0.3644074735819558 1104 18-19 0.33905122237854357 1104 20-21 0.3137605124937082 1104 22-23 0.3130682365315991 1104 24-25 0.37651053271134316 1104 26-27 0.3245321167445212 1104 28-29 0.3928629902469041 1104 30-31 0.3698643635706489 1104 32-33 0.36996051010370934 1104 34-35 0.3247532246762148 1104 36-37 0.32496688991970046 1104 38-39 0.3298037604342241 1104 40-41 0.27457384770296045 1104 42-43 0.34116005067224364 1104 44-45 0.3004489478132797 1104 46-47 0.33379580855522306 1104 48-49 0.28410377185996794 1104 50-51 0.29640660248764306 1104 52-53 0.27682331678234817 1104 54-55 0.2662393400233327 1104 56-57 0.25354868536260966 1104 58-59 0.300801808526586 1104 60-61 0.27534977064973276 1104 62-63 0.3062075789031482 1104 64-65 0.34173467878054 1104 66-67 0.3607217983475337 1104 68-69 0.3094863072332217 1104 70-71 0.3404568346401575 1104 72-73 0.31977449892565346 1104 74-75 0.3398955696826249 1104 76-77 0.28392001731123884 1104 78-79 0.30689184457385466 1104 80-81 0.31053895838076073 1104 82-83 0.24743667808913017 1104 84-85 0.27902382631163647 1104 86-87 0.21873179360235184 1104 88-89 0.20360035368749152 1104 90-91 0.2614228762487727 1104 92-93 0.21657195556238662 1104 94-95 0.2576105482745632 1104 96-97 0.22807675009498496 1104 98-99 0.2789869153732738 1104 100 0.3345375808246267 1105 1 0.12750929063033922 1105 2 0.12517247176191404 1105 3 0.015311539917121308 1105 4 0.09631130313272962 1105 5 0.03089516945492221 1105 6 0.03125768201825707 1105 7 0.08780054239465329 1105 8 0.1347816923898506 1105 9 0.17159412865412094 1105 10-11 0.17257814536782234 1105 12-13 0.09647023494649432 1105 14-15 0.17351483709698812 1105 16-17 0.21201640265101673 1105 18-19 0.21869159720787934 1105 20-21 0.18346599828949195 1105 22-23 0.16990901543942272 1105 24-25 0.2816825558671141 1105 26-27 0.2622255644906488 1105 28-29 0.2214061122479265 1105 30-31 0.30340185353016835 1105 32-33 0.25023393342481626 1105 34-35 0.2976862595855039 1105 36-37 0.25060487083867145 1105 38-39 0.2613301959022678 1105 40-41 0.2868419065337875 1105 42-43 0.2695150378976976 1105 44-45 0.16484458058075546 1105 46-47 0.2345910124210775 1105 48-49 0.22525032846959903 1105 50-51 0.24048103032608736 1105 52-53 0.17857946577736783 1105 54-55 0.17325120243759784 1105 56-57 0.23323692083580738 1105 58-59 0.20055512862091263 1105 60-61 0.2277526384243842 1105 62-63 0.1874141384166137 1105 64-65 0.23478453756968776 1105 66-67 0.2111915205626005 1105 68-69 0.1997734945144103 1105 70-71 0.11027245846194234 1105 72-73 0.06814329987454926 1105 74-75 0.1820840539726163 1105 76-77 0.10927474225451306 1105 78-79 0.11533159343866828 1105 80-81 0.03895797934226408 1105 82-83 0.10475988350525256 1105 84-85 0.08505700442231756 1105 86-87 8.568193571534266E-5 1105 88-89 0.05240619403257796 1105 90-91 0.015858679757272398 1105 92-93 0.13195858174704966 1105 94-95 0.16638873190019154 1105 96-97 0.053220566207073716 1105 98-99 0.061269080653204355 1105 100 0.18009661070840366 1106 1 0.0765714401870099 1106 2 0.11422267141010778 1106 3 0.0974552888441913 1106 4 0.03663329854450126 1106 5 -0.008388482579789525 1106 6 0.006520758361673984 1106 7 0.03899622994913443 1106 8 0.09860089205810851 1106 9 0.1291473458771435 1106 10-11 0.1519698064051056 1106 12-13 0.16282759183184936 1106 14-15 0.21392631748108215 1106 16-17 0.19197001315088613 1106 18-19 0.196212866261412 1106 20-21 0.24266967121425154 1106 22-23 0.26656205174172953 1106 24-25 0.2637346460978378 1106 26-27 0.2537042179633744 1106 28-29 0.24481659048951343 1106 30-31 0.1866343316424306 1106 32-33 0.18159048770437636 1106 34-35 0.22604084525141133 1106 36-37 0.29122387219641865 1106 38-39 0.19017863494546816 1106 40-41 0.1948643276610511 1106 42-43 0.24885501725288606 1106 44-45 0.24824354365865986 1106 46-47 0.23087699591415145 1106 48-49 0.23249985136504137 1106 50-51 0.26012894555753974 1106 52-53 0.1988958986850875 1106 54-55 0.12991051745814275 1106 56-57 0.20582559205503514 1106 58-59 0.1634775556562431 1106 60-61 0.24592212870282282 1106 62-63 0.17245859664655683 1106 64-65 0.19388841426714265 1106 66-67 0.1971229139308477 1106 68-69 0.14654718643416942 1106 70-71 0.181225569562649 1106 72-73 0.20700378636513506 1106 74-75 0.1489592170019236 1106 76-77 0.18844422361510738 1106 78-79 0.21983164806044897 1106 80-81 0.21937566598712976 1106 82-83 0.19189235693566076 1106 84-85 0.1882010872111053 1106 86-87 0.13887934034669058 1106 88-89 0.14829856626776916 1106 90-91 0.13876634497701446 1106 92-93 0.21026306028737451 1106 94-95 0.18012960783294574 1106 96-97 0.21268214591363233 1106 98-99 0.16905661417871443 1106 100 0.24060408608958284 1107 1 0.11107969494300818 1107 2 0.17443904858132342 1107 3 0.14139229463410175 1107 4 0.15285205784971367 1107 5 0.18774584330937216 1107 6 0.1568974663848337 1107 7 0.1527547742005808 1107 8 0.11515473821195599 1107 9 0.22966016638996933 1107 10-11 0.21281989738939444 1107 12-13 0.23999007322142774 1107 14-15 0.29120400151747816 1107 16-17 0.23671489321705508 1107 18-19 0.1916544543507399 1107 20-21 0.22668694168572046 1107 22-23 0.26137621220492946 1107 24-25 0.33752627554365944 1107 26-27 0.24828850249603818 1107 28-29 0.2501578642694966 1107 30-31 0.3091252497565833 1107 32-33 0.34847366388303413 1107 34-35 0.30993828114884536 1107 36-37 0.29083260668772937 1107 38-39 0.28989105843595553 1107 40-41 0.24870333510521903 1107 42-43 0.3141258195688508 1107 44-45 0.27957423017644345 1107 46-47 0.33079917953698157 1107 48-49 0.37257515326744084 1107 50-51 0.29414165027218075 1107 52-53 0.26895558437574607 1107 54-55 0.22076715931090973 1107 56-57 0.25819799900292395 1107 58-59 0.2590379857637686 1107 60-61 0.30537840661845195 1107 62-63 0.24600413841660895 1107 64-65 0.28441513056160517 1107 66-67 0.22173677662728863 1107 68-69 0.23248842712896334 1107 70-71 0.22675132638647 1107 72-73 0.27229119744867347 1107 74-75 0.2727347008728884 1107 76-77 0.18235873147283854 1107 78-79 0.17643498966508275 1107 80-81 0.13908894969267038 1107 82-83 0.2035338457694067 1107 84-85 0.18019810954361049 1107 86-87 0.17156738004891992 1107 88-89 0.12050481936950064 1107 90-91 0.12455552611845633 1107 92-93 0.19501998336429693 1107 94-95 0.2111461173449385 1107 96-97 0.19907129396987955 1107 98-99 0.19957770599013003 1107 100 0.21435283712349573 1108 1 0.060737707228408766 1108 2 0.07181854141483868 1108 3 0.005577797862958533 1108 4 0.05392478111321708 1108 5 0.06415623864722164 1108 6 0.05947301529017324 1108 7 0.0181050844856685 1108 8 0.032957604805119445 1108 9 -0.021106883539154353 1108 10-11 -0.005056517794102433 1108 12-13 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-0.22988510159900954 2214 84-85 -0.2822990196429913 2214 86-87 -0.2198074524869611 2214 88-89 -0.30273613756828865 2214 90-91 -0.2971029427810663 2214 92-93 -0.2741959018031679 2214 94-95 -0.26814316495171653 2214 96-97 -0.28705061990571323 2214 98-99 -0.2712954997514956 2214 100 -0.3050358710104746 2215 1 -0.3143889084089473 2215 2 -0.34561458270359324 2215 3 -0.3494850238016518 2215 4 -0.31243777455252086 2215 5 -0.3072172851822472 2215 6 -0.2635530364084673 2215 7 -0.3088121531737187 2215 8 -0.28518939586625436 2215 9 -0.43648508500668015 2215 10-11 -0.43018881769440753 2215 12-13 -0.4304269100188023 2215 14-15 -0.5336916409406243 2215 16-17 -0.5416725928164681 2215 18-19 -0.5955482272135058 2215 20-21 -0.5719012147388653 2215 22-23 -0.5096065810911625 2215 24-25 -0.4368339479200287 2215 26-27 -0.4631885924760297 2215 28-29 -0.5108231413170969 2215 30-31 -0.5757525156065526 2215 32-33 -0.5870713640499261 2215 34-35 -0.5280058529293683 2215 36-37 -0.4989090692899225 2215 38-39 -0.5727788856981135 2215 40-41 -0.5221914134981276 2215 42-43 -0.5618205491462263 2215 44-45 -0.48541347932076917 2215 46-47 -0.5273545634797827 2215 48-49 -0.45957143891133256 2215 50-51 -0.4554681821300548 2215 52-53 -0.4081670971885032 2215 54-55 -0.49878636024216405 2215 56-57 -0.5458659116115996 2215 58-59 -0.3997182153535448 2215 60-61 -0.42978271069998186 2215 62-63 -0.43808167163925305 2215 64-65 -0.4347513498853246 2215 66-67 -0.4521792010263397 2215 68-69 -0.4895772711950599 2215 70-71 -0.4001304613230303 2215 72-73 -0.36721092545634804 2215 74-75 -0.3170382600209649 2215 76-77 -0.29967389422548507 2215 78-79 -0.30255182597737473 2215 80-81 -0.3060069162291228 2215 82-83 -0.36014972964959213 2215 84-85 -0.30402758878040714 2215 86-87 -0.27986859760471106 2215 88-89 -0.2705910465522834 2215 90-91 -0.3170015688536125 2215 92-93 -0.3707382289262071 2215 94-95 -0.3376570111466819 2215 96-97 -0.3030118345217403 2215 98-99 -0.3745005063003717 2215 100 -0.38140939751337655 2216 1 -0.23814776774355906 2216 2 -0.2680462392651606 2216 3 -0.27235491218892705 2216 4 -0.17443084193706682 2216 5 -0.24663027609361166 2216 6 -0.22725400483052027 2216 7 -0.2599430509593361 2216 8 -0.28328364131895967 2216 9 -0.33925241356738667 2216 10-11 -0.4062721281969601 2216 12-13 -0.35640873274872575 2216 14-15 -0.49573527353582847 2216 16-17 -0.4989779852477625 2216 18-19 -0.4748318569498977 2216 20-21 -0.477343079636249 2216 22-23 -0.5076538944048821 2216 24-25 -0.4888815709382115 2216 26-27 -0.6192240774613111 2216 28-29 -0.6052719864767724 2216 30-31 -0.5895513387295637 2216 32-33 -0.5900831804666495 2216 34-35 -0.5921128915590401 2216 36-37 -0.5509613378751723 2216 38-39 -0.4903906473209645 2216 40-41 -0.5416095860035242 2216 42-43 -0.5153483808575885 2216 44-45 -0.40003301929050394 2216 46-47 -0.4279102447700609 2216 48-49 -0.39809998532530955 2216 50-51 -0.4072693518600161 2216 52-53 -0.4090392130656184 2216 54-55 -0.3623690453799213 2216 56-57 -0.3406225553680784 2216 58-59 -0.37287237670958007 2216 60-61 -0.37762210510862104 2216 62-63 -0.3724605950588682 2216 64-65 -0.3531622681590818 2216 66-67 -0.341750558127508 2216 68-69 -0.4437746005895846 2216 70-71 -0.45502956913592385 2216 72-73 -0.3414714464745643 2216 74-75 -0.331226834308346 2216 76-77 -0.41836033036084785 2216 78-79 -0.3618166947698853 2216 80-81 -0.3628380439107417 2216 82-83 -0.3459607384523409 2216 84-85 -0.32138056849476726 2216 86-87 -0.34185999734980044 2216 88-89 -0.33776332561983224 2216 90-91 -0.32010528411608163 2216 92-93 -0.230732511305213 2216 94-95 -0.3088503858533542 2216 96-97 -0.3504951879064535 2216 98-99 -0.328513978444839 2216 100 -0.3298387193797012 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 1307.0 1 334.0 2 249.0 3 247.0 4 187.0 5 264.0 6 392.0 7 964.0 8 2801.0 9 4160.0 10 4353.0 11 5248.0 12 6450.0 13 8082.0 14 9746.0 15 10737.0 16 11334.0 17 12764.0 18 14232.0 19 15868.0 20 17774.0 21 19773.0 22 21965.0 23 25380.0 24 30136.0 25 37041.0 26 41482.0 27 39931.0 28 42871.0 29 52589.0 30 61524.0 31 70033.0 32 88309.0 33 116752.0 34 170382.0 35 256626.0 36 463126.0 37 977801.0 38 1300981.0 39 416604.0 40 982.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.82196717082818 20.46834100369343 13.408549831484628 24.30114199399376 2 14.528402178583564 20.951213575472604 41.50391639877136 23.01646784717247 3 17.591576642248675 27.03419390573582 30.511635142632542 24.86259430938296 4 11.976357048944855 18.008430143844777 40.75281597551266 29.262396831697707 5 12.759733860344369 38.7507305256825 36.04041427546423 12.44912133850891 6 28.764090477369532 39.59198397179874 19.095813754540973 12.548111796290758 7 25.715291422927923 33.05901657626161 24.042084645199072 17.183607355611397 8 24.5476853855442 36.33195943567548 21.538542028555838 17.581813150224487 9 25.85811994716615 16.014820460568597 21.323030563471633 36.80402902879362 10-11 23.867171595319626 27.03536600042288 29.60686304642046 19.490599357837034 12-13 24.39557131807053 24.866684018841756 28.93988343146795 21.797861231619763 14-15 22.004906496863573 26.146438189359483 26.41980146859913 25.428853845177812 16-17 21.807382358073475 28.820013333441942 27.10706414289613 22.265540165588455 18-19 21.595126019762542 28.807765511117434 28.331484347483908 21.26562412163612 20-21 22.85899173684172 27.915507279361563 27.679664499621897 21.54583648417482 22-23 22.727872920688313 27.8850809927902 27.321473824313657 22.065572262207827 24-25 22.30193798912061 28.09152675840207 27.651346891425437 21.95518836105189 26-27 22.230271402291827 28.267741501786574 27.30267731406853 22.19930978185307 28-29 21.972363375707868 28.44073356701337 27.492827664204246 22.09407539307452 30-31 22.497092467284592 28.102810953410295 27.431104066209027 21.968992513096087 32-33 22.393026186792504 28.28444355719086 27.084551633856453 22.23797862216019 34-35 22.52836537143202 28.22388160564564 27.44085726404891 21.80689575887343 36-37 22.46128015315495 28.048737253006838 27.395010876126207 22.094971717712006 38-39 22.2879050550782 28.19275131686284 27.46737817225977 22.051965455799188 40-41 22.383741408170827 27.760691958472393 27.73220454490697 22.123362088449806 42-43 22.251745138624358 27.86067345599405 27.521077052287612 22.366504353093976 44-45 22.252151908493637 28.206384958944398 27.45943544641661 22.082027686145363 46-47 22.244696131836058 27.990197653127254 27.33308246178416 22.432023753252533 48-49 22.337658880620356 27.796779670623827 27.572696295544972 22.29286515321084 50-51 22.304550101786095 27.848235170340118 27.365687731066714 22.481526996807073 52-53 22.183081824950442 27.99167308159432 27.374704099629287 22.45054099382595 54-55 22.129821740229858 27.880194271621388 27.400928678193914 22.589055309954837 56-57 22.304299501382538 27.876499096609386 27.344205994401467 22.47499540760661 58-59 22.180360023325026 27.811975120887855 27.589051740300917 22.4186131154862 60-61 22.22866048088973 28.032088995144377 27.387266154477054 22.351984369488832 62-63 22.283865941261954 27.75488847849173 27.340597707321802 22.620647872924515 64-65 21.936296263487808 27.750926879050702 27.46781726538351 22.84495959207798 66-67 22.229894989044503 27.726552688212756 27.31350844912766 22.73004387361507 68-69 22.076758402732906 27.677513229607747 27.470218107376947 22.7755102602824 70-71 22.128561332551378 27.386687911529815 27.521973596686404 22.9627771592324 72-73 22.237078868020742 27.562798173569636 27.54220183675722 22.657921121652407 74-75 22.158268576777097 27.74821829346083 27.45423628419592 22.639276845566155 76-77 22.087583202114313 27.60730747751625 27.411325183392037 22.893784136977395 78-79 22.134492079615573 27.58550255919002 27.34352587579563 22.936479485398777 80-81 22.074478339056895 27.625941266269923 27.57661170764038 22.72296868703281 82-83 22.345303448972285 27.256921812890727 27.87894401042862 22.518830727708366 84-85 22.525658324510232 27.366416238650217 27.370627459246688 22.737297977592863 86-87 21.9925095867651 27.59418666825777 27.653852950153414 22.75945079482372 88-89 22.34497444008221 27.184675339934515 27.523921402679086 22.946428817304188 90-91 22.365539356600117 27.175611356334834 27.569556581276487 22.88929270578856 92-93 22.535555638165704 27.13168608693617 27.517521383407935 22.81523689149019 94-95 22.06496359996099 27.05631823214562 27.78173744930097 23.096980718592416 96-97 22.197467831594164 27.02259676336789 27.653608081827937 23.126327323210013 98-99 22.044138997173953 27.19027678902152 27.539906355411553 23.225677858392967 100 22.45044293059965 26.977238243942082 27.196141059365527 23.376177766092745 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1469.0 1 953.0 2 435.5 3 422.0 4 402.5 5 399.5 6 371.0 7 341.5 8 359.5 9 382.5 10 377.0 11 369.0 12 393.5 13 407.5 14 450.0 15 540.0 16 656.5 17 823.5 18 1097.5 19 1488.0 20 1995.5 21 2848.5 22 4224.0 23 6101.0 24 8856.5 25 13605.5 26 20529.5 27 27820.0 28 34540.5 29 42838.5 30 52988.0 31 63863.5 32 76930.0 33 89477.0 34 100621.0 35 110040.5 36 120575.5 37 134824.5 38 143143.0 39 144628.5 40 145174.0 41 145788.0 42 148351.0 43 154516.5 44 162123.0 45 171954.0 46 180109.5 47 185946.5 48 190178.5 49 185846.0 50 175599.0 51 168597.5 52 161238.5 53 151352.5 54 140062.0 55 124754.5 56 110512.0 57 93467.0 58 77835.0 59 67420.0 60 54069.0 61 41302.5 62 31971.5 63 24329.5 64 17547.5 65 11702.0 66 7993.0 67 5842.0 68 4208.5 69 2866.5 70 2074.0 71 1421.5 72 960.0 73 724.5 74 532.5 75 388.0 76 269.5 77 159.5 78 105.5 79 82.5 80 77.0 81 58.0 82 39.0 83 39.0 84 39.0 85 32.0 86 29.5 87 28.0 88 21.0 89 18.5 90 21.5 91 21.5 92 17.5 93 13.0 94 13.0 95 14.5 96 14.0 97 9.0 98 9.0 99 12.5 100 15.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09892747939431164 2 0.06423981396590062 3 0.0806780532997874 4 0.08278728345141582 5 0.08294776835425713 6 0.09083445500817212 7 0.08716622865751399 8 0.0885647399537024 9 0.0736396439894621 10-11 0.0811938976303487 12-13 0.07009751291960784 14-15 0.07973807029743125 16-17 0.08176705799763904 18-19 0.08106780234954483 20-21 0.07237869118142337 22-23 0.07651690903325958 24-25 0.07907320427137447 26-27 0.0724245440108066 28-29 0.08121682404504031 30-31 0.08493090322508168 32-33 0.08628356169188688 34-35 0.0887366880638895 36-37 0.0880718220378327 38-39 0.08909204749160951 40-41 0.08519455699403523 42-43 0.0908115285934805 44-45 0.08686818526652301 46-47 0.08347507589216423 48-49 0.09234759837781861 50-51 0.08866790881981466 52-53 0.09414732193111025 54-55 0.08618039282577461 56-57 0.09242784082923924 58-59 0.0969787341455245 60-61 0.09514462097019544 62-63 0.09660044830311287 64-65 0.09815944450214259 66-67 0.10038330672722907 68-69 0.09687556527941224 70-71 0.09939747089548971 72-73 0.09652020585169223 74-75 0.10022282182438778 76-77 0.0973111671585529 78-79 0.10415470194399948 80-81 0.09979868315259295 82-83 0.10410884911461625 84-85 0.10029160106846263 86-87 0.09654313226638385 88-89 0.09576363416686899 90-91 0.09618777283866384 92-93 0.09085738142286373 94-95 0.09069689652002244 96-97 0.08840425505086111 98-99 0.1021371774511375 100 0.2415297787761467 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4361781.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.79535610701445 #Duplication Level Percentage of deduplicated Percentage of total 1 68.4331640609589 36.12953266128068 2 15.370750611185303 16.23008504299276 3 6.5357000714420685 10.351638380412714 4 3.340843401279012 7.055240682731792 5 1.9213146010870663 5.071824427899904 6 1.164052716617225 3.6873946620686384 7 0.8220278417396312 3.0379476844167037 8 0.5446674693089831 2.3004730401659303 9 0.37634573416841777 1.7882376349299671 >10 1.4410845188279444 11.80847151018012 >50 0.03675127649490652 1.287977239955231 >100 0.013054935000249445 1.167099478039993 >500 2.4276189026921894E-4 0.08407755492556691 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.5852829383226716E-5 2 0.0 0.0 0.0 0.0 4.5852829383226716E-5 3 0.0 0.0 0.0 0.0 4.5852829383226716E-5 4 0.0 0.0 0.0 0.0 4.5852829383226716E-5 5 0.0 0.0 0.0 0.0 4.5852829383226716E-5 6 2.2926414691613358E-5 0.0 0.0 0.0 1.1463207345806679E-4 7 2.2926414691613358E-5 0.0 0.0 0.0 1.3755848814968013E-4 8 2.2926414691613358E-5 0.0 0.0 0.0 1.3755848814968013E-4 9 2.2926414691613358E-5 0.0 0.0 0.0 1.8341131753290687E-4 10-11 4.5852829383226716E-5 0.0 0.0 2.2926414691613358E-5 1.8341131753290687E-4 12-13 9.170565876645343E-5 0.0 0.0 2.2926414691613358E-5 1.9487452487871354E-4 14-15 1.2609528080387346E-4 0.0 0.0 2.2926414691613358E-5 3.4389622037420034E-4 16-17 1.3755848814968013E-4 0.0 0.0 2.2926414691613358E-5 4.126754644490404E-4 18-19 1.3755848814968013E-4 0.0 0.0 2.2926414691613358E-5 4.5852829383226715E-4 20-21 1.604849028412935E-4 0.0 0.0 3.438962203742004E-5 4.5852829383226715E-4 22-23 1.604849028412935E-4 0.0 0.0 1.1463207345806679E-4 5.158443305613006E-4 24-25 1.604849028412935E-4 0.0 0.0 3.668226350658137E-4 6.07549989327754E-4 26-27 1.8341131753290687E-4 0.0 0.0 9.170565876645343E-4 6.41939611365174E-4 28-29 2.063377322245202E-4 0.0 0.0 0.0029804339099097363 6.41939611365174E-4 30-31 2.4072735426194025E-4 0.0 0.0 0.01035127623326343 6.534028187109807E-4 32-33 2.521905616077469E-4 0.0 0.0 0.03091627021164061 7.107188554400141E-4 34-35 2.521905616077469E-4 0.0 0.0 0.061752297971860576 7.336452701316275E-4 36-37 2.521905616077469E-4 0.0 0.0 0.11192675652445641 7.909613068606608E-4 38-39 2.751169762993603E-4 0.0 0.0 0.18844366555771597 8.482773435896942E-4 40-41 3.20969805682587E-4 0.0 0.0 0.2764467083514739 8.94130172972921E-4 42-43 3.20969805682587E-4 0.0 0.0 0.3706513463193132 8.94130172972921E-4 44-45 3.20969805682587E-4 0.0 0.0 0.4857419480712122 9.055933803187276E-4 46-47 3.20969805682587E-4 0.0 0.0 0.6218446088879749 9.28519795010341E-4 48-49 3.4389622037420034E-4 0.0 0.0 0.776540592019636 9.62909417047761E-4 50-51 3.8974904975742707E-4 0.0 0.0 0.9465399569579491 9.62909417047761E-4 52-53 3.8974904975742707E-4 0.0 0.0 1.1160349407730465 0.0010431518684684078 54-55 3.8974904975742707E-4 0.0 0.0 1.3020140167514143 0.0011233943198890545 56-57 3.8974904975742707E-4 0.0 0.0 1.5222566194863978 0.0011348575272348612 58-59 4.356018791406538E-4 0.0 0.0 1.7774046885893628 0.0011921735639638946 60-61 4.470650864864605E-4 0.0 0.0 2.046022026323651 0.0012150999786555078 62-63 4.929179158696872E-4 0.0 0.0 2.3219987431739466 0.0013068056374219614 64-65 5.387707452529139E-4 0.0 0.0 2.6091176975643666 0.001318268844767768 66-67 5.502339525987205E-4 0.0 0.0 2.9356586220170158 0.0013641216741509948 68-69 5.73160367290334E-4 0.0 0.0 3.301965412752268 0.0013985112961884148 70-71 5.960867819819473E-4 0.0 0.0 3.702455487792716 0.0014443641255716415 72-73 5.960867819819473E-4 0.0 0.0 4.112586120210987 0.0015589961990297083 74-75 5.960867819819473E-4 0.0 0.0 4.523771367705073 0.0015589961990297083 76-77 5.960867819819473E-4 0.0 0.0 4.973828351308789 0.001570459406375515 78-79 5.960867819819473E-4 0.0 0.0 5.470185229382218 0.0015819226137213217 80-81 6.190131966735606E-4 0.0 0.0 6.010503507626815 0.0016163122357587417 82-83 6.304764040193673E-4 0.0 0.0 6.568085376134198 0.0016965546871793883 84-85 6.41939611365174E-4 0.0 0.0 7.118605908916564 0.0016965546871793883 86-87 6.41939611365174E-4 0.0 0.0 7.691158726217571 0.0016965546871793883 88 6.41939611365174E-4 2.2926414691613358E-5 0.0 8.14495271541602 0.0017424075165626152 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3925 0.0 37.50317 1 GTATCAA 6270 0.0 36.527897 1 CAACGCA 6765 0.0 31.75938 5 ATCAACG 6880 0.0 31.229595 3 AACGCAG 7650 0.0 28.872913 6 TATCAAC 7980 0.0 28.633339 2 TCAACGC 7720 0.0 28.013937 4 ACGCAGA 8275 0.0 25.8962 7 CGCAGAG 8555 0.0 24.992268 8 GTACATG 14425 0.0 24.549486 1 ACATGGG 13710 0.0 24.210617 3 TACATGG 14215 0.0 24.177374 2 GTACTAG 1955 0.0 23.815062 1 CATGGGG 9880 0.0 21.413574 4 GCATCGT 1860 0.0 20.463804 8 GCAGAGT 10470 0.0 19.746965 9 GTATAAG 2615 0.0 19.063272 1 CGACGAG 965 0.0 18.9916 24-25 GTCGCCG 895 0.0 18.93594 94 TAACGCC 525 0.0 18.805878 4 >>END_MODULE