FastQCFastQC Report
Mon 16 Jan 2017
ERR1146631_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146631_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4361781
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107200.24577116549409517No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97520.22357839607261343No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53050.12162462993900885No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71600.057.0852361
GTATCAA133000.044.0285841
ATCAACG166050.034.866343
CAACGCA167950.034.505345
TATCAAC174650.033.501472
AACGCAG178800.032.7524536
TCAACGC178150.032.444664
ACGCAGA196350.029.4649377
CGCAGAG199550.028.8982268
GCAGAGT225650.025.1596749
GTACATG135200.022.9258671
TACATGG133000.022.4207252
GAGTACT151450.022.29645712-13
AGAGTAC204650.022.16123410-11
ACATGGG130150.021.9708333
CAGAGTA218500.021.89649810-11
GTGGTAT26450.020.0941581
GTACTTT165850.020.0771814-15
AGTACTT165900.018.9096412-13
CTAGTAC26900.018.7076243
Produced by FastQC (version 0.11.5)