##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146631_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4361781
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.344939601506816	33.0	31.0	34.0	30.0	34.0
2	32.43981139814218	34.0	31.0	34.0	30.0	34.0
3	32.54061861427706	34.0	31.0	34.0	30.0	34.0
4	36.028725192759566	37.0	35.0	37.0	35.0	37.0
5	36.06609983398983	37.0	35.0	37.0	35.0	37.0
6	36.12890789335824	37.0	36.0	37.0	35.0	37.0
7	36.09395198887794	37.0	36.0	37.0	35.0	37.0
8	36.10209934886689	37.0	36.0	37.0	35.0	37.0
9	37.89367943966008	39.0	38.0	39.0	35.0	39.0
10-11	37.838986872564206	39.0	38.0	39.0	35.0	39.0
12-13	37.80982814130283	39.0	38.0	39.0	35.0	39.0
14-15	39.16821305333761	40.0	39.0	41.0	36.0	41.0
16-17	39.12125643171906	40.0	39.0	41.0	36.0	41.0
18-19	39.21877221712874	40.0	39.0	41.0	36.0	41.0
20-21	39.27467002584494	41.0	39.0	41.0	36.0	41.0
22-23	39.24017906905459	40.5	39.0	41.0	36.0	41.0
24-25	39.231694690769665	41.0	39.0	41.0	36.0	41.0
26-27	39.15954148087673	41.0	39.0	41.0	36.0	41.0
28-29	39.101390922652925	40.0	39.0	41.0	35.0	41.0
30-31	38.97228139147747	40.0	38.5	41.0	35.0	41.0
32-33	38.875203500588405	40.0	38.0	41.0	35.0	41.0
34-35	38.80881639862249	40.0	38.0	41.0	35.0	41.0
36-37	38.749504732126624	40.0	38.0	41.0	35.0	41.0
38-39	38.67059625414481	40.0	38.0	41.0	35.0	41.0
40-41	38.57990371364358	40.0	38.0	41.0	35.0	41.0
42-43	38.485344174776316	40.0	38.0	41.0	34.5	41.0
44-45	38.35155627941889	40.0	38.0	41.0	34.0	41.0
46-47	38.13407447554107	40.0	38.0	41.0	33.5	41.0
48-49	37.88061470761599	40.0	38.0	41.0	33.0	41.0
50-51	37.78270149280764	40.0	37.5	41.0	33.0	41.0
52-53	37.694723783702116	40.0	37.0	41.0	33.0	41.0
54-55	37.54524447696939	40.0	37.0	41.0	33.0	41.0
56-57	37.412053356186384	40.0	36.5	41.0	32.5	41.0
58-59	37.25275512456953	39.5	36.0	41.0	32.0	41.0
60-61	37.06667345288541	39.0	35.5	41.0	32.0	41.0
62-63	36.841818743306916	39.0	35.0	41.0	32.0	41.0
64-65	36.57966482040249	39.0	35.0	41.0	31.0	41.0
66-67	36.32079499177056	38.0	35.0	40.0	31.0	41.0
68-69	36.03803171227533	37.0	35.0	40.0	31.0	41.0
70-71	35.734346359892896	37.0	35.0	39.5	31.0	41.0
72-73	35.40701114980325	36.0	35.0	39.0	31.0	41.0
74-75	35.07894252370764	36.0	35.0	39.0	30.0	40.5
76-77	34.76337567154334	35.5	35.0	38.0	30.0	40.0
78-79	34.46251049743213	35.0	34.0	37.0	30.0	39.0
80-81	34.18201830857625	35.0	34.0	37.0	30.0	39.0
82-83	33.91001703203348	35.0	34.0	36.0	30.0	38.5
84-85	33.679268629030204	35.0	34.0	36.0	29.5	37.0
86-87	33.482560564136534	35.0	34.0	36.0	29.0	37.0
88-89	33.27904759546617	35.0	34.0	35.0	29.0	36.5
90-91	33.181738606317005	35.0	34.0	35.0	29.0	36.0
92-93	33.076351265687116	35.0	34.0	35.0	29.0	36.0
94-95	32.992731638750314	35.0	34.0	35.0	29.0	36.0
96-97	32.93222069150193	35.0	34.0	35.0	29.0	36.0
98-99	32.86237055459685	35.0	34.0	35.0	29.0	35.0
100	32.13730973655028	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.36796017223321087
1101	2	0.31185018707211754
1101	3	0.35366281786605924
1101	4	0.22159134629653465
1101	5	0.20817286469199558
1101	6	0.23572059283451097
1101	7	0.24359717547002901
1101	8	0.22412274595720305
1101	9	0.32581626327579727
1101	10-11	0.29831437878779354
1101	12-13	0.3295977537805257
1101	14-15	0.45140689967887937
1101	16-17	0.4296497335849452
1101	18-19	0.4022299606555251
1101	20-21	0.375683104209962
1101	22-23	0.3811233557823286
1101	24-25	0.38730995501617826
1101	26-27	0.36522371667376063
1101	28-29	0.4008697975830131
1101	30-31	0.46987845115546634
1101	32-33	0.4013735072505824
1101	34-35	0.40842552778639174
1101	36-37	0.45841239725315575
1101	38-39	0.42964801162649024
1101	40-41	0.4253012123403508
1101	42-43	0.4794936259510507
1101	44-45	0.489159923057791
1101	46-47	0.44256629172855355
1101	48-49	0.5215161644388502
1101	50-51	0.49587838150220165
1101	52-53	0.4680075518956315
1101	54-55	0.46489284850567714
1101	56-57	0.4547619431486041
1101	58-59	0.41128215868909024
1101	60-61	0.39877115985465394
1101	62-63	0.3776619764133571
1101	64-65	0.36615968952548883
1101	66-67	0.32021024783223595
1101	68-69	0.2951498026850601
1101	70-71	0.26952982862880503
1101	72-73	0.31075103505614976
1101	74-75	0.3651147368898222
1101	76-77	0.3302069829309673
1101	78-79	0.23572170034593398
1101	80-81	0.2436352963897832
1101	82-83	0.29281676517408073
1101	84-85	0.25441630380876745
1101	86-87	0.24672248797926244
1101	88-89	0.37117745054914053
1101	90-91	0.30176943986761273
1101	92-93	0.2856535065456782
1101	94-95	0.3131989701255904
1101	96-97	0.2994549581426398
1101	98-99	0.29128697241593215
1101	100	0.31999218239189275
1102	1	0.28307405833735544
1102	2	0.27816495890175474
1102	3	0.25831060197473477
1102	4	0.18065596332651523
1102	5	0.1658387379183779
1102	6	0.17560899173076194
1102	7	0.15911715186633302
1102	8	0.2033299586127697
1102	9	0.21175856134642146
1102	10-11	0.22109520208746858
1102	12-13	0.2555206579124345
1102	14-15	0.38009145889392926
1102	16-17	0.40843539432778186
1102	18-19	0.34403967118917933
1102	20-21	0.33145734861628995
1102	22-23	0.3522509020622735
1102	24-25	0.29998990251026214
1102	26-27	0.24072246749711468
1102	28-29	0.2832869160463787
1102	30-31	0.2913816239673608
1102	32-33	0.2762100115322639
1102	34-35	0.3387991249151199
1102	36-37	0.30780719797662925
1102	38-39	0.2903409242397572
1102	40-41	0.27010356233895294
1102	42-43	0.3255791856325416
1102	44-45	0.30668102100685957
1102	46-47	0.30995649701380046
1102	48-49	0.36751746265591123
1102	50-51	0.335149594435066
1102	52-53	0.3050977678485154
1102	54-55	0.22658327499517839
1102	56-57	0.22898248441992308
1102	58-59	0.22129095311910874
1102	60-61	0.17004116968125516
1102	62-63	0.21414503112362837
1102	64-65	0.2219059318013592
1102	66-67	0.20987275157840912
1102	68-69	0.19217238723837227
1102	70-71	0.1520826599380598
1102	72-73	0.18591812101518457
1102	74-75	0.2354073591530721
1102	76-77	0.19816311847063872
1102	78-79	0.13307483163507783
1102	80-81	0.13735594267783569
1102	82-83	0.0675103796226253
1102	84-85	0.06978951186373195
1102	86-87	0.12950876355300522
1102	88-89	0.1393928206858419
1102	90-91	0.11170214914781695
1102	92-93	0.07352187179395031
1102	94-95	0.1245203775237087
1102	96-97	0.1034772402072548
1102	98-99	0.12842781862466524
1102	100	0.20645189442279133
1103	1	0.21282326939834206
1103	2	0.18270516369065604
1103	3	0.16633068203101686
1103	4	0.13926997316274736
1103	5	0.1112655934568707
1103	6	0.12611155740066948
1103	7	0.14681691916434403
1103	8	0.17747185093256235
1103	9	0.18973156595040308
1103	10-11	0.197402204084689
1103	12-13	0.23235995891160854
1103	14-15	0.3463631625999142
1103	16-17	0.3795630609841396
1103	18-19	0.30219715920257073
1103	20-21	0.28339853556772
1103	22-23	0.29940582911902425
1103	24-25	0.28857154623045744
1103	26-27	0.3067543130308934
1103	28-29	0.30697613016899084
1103	30-31	0.3289680341816563
1103	32-33	0.3108783199161138
1103	34-35	0.31557780085180553
1103	36-37	0.3600069533919239
1103	38-39	0.2849951977473779
1103	40-41	0.29611300862983114
1103	42-43	0.37458926283564153
1103	44-45	0.36073491089541676
1103	46-47	0.3423661940498022
1103	48-49	0.2994523623917189
1103	50-51	0.2215644655620821
1103	52-53	0.2371429477760003
1103	54-55	0.28356937328498333
1103	56-57	0.30444538573194535
1103	58-59	0.2702231620514226
1103	60-61	0.2887893698487787
1103	62-63	0.2856787545904922
1103	64-65	0.2275500703519171
1103	66-67	0.22550825889914705
1103	68-69	0.1469851243366378
1103	70-71	0.20071570999374444
1103	72-73	0.2535484069796041
1103	74-75	0.20215480281009945
1103	76-77	0.1705794784549255
1103	78-79	0.2569104420897972
1103	80-81	0.2161426673923188
1103	82-83	0.14713279418693048
1103	84-85	0.19075558028345085
1103	86-87	0.2492490373545735
1103	88-89	0.3042515007277018
1103	90-91	0.25475321586345956
1103	92-93	0.22554041917961598
1103	94-95	0.2037219847832361
1103	96-97	0.21800404076663682
1103	98-99	0.18587321026356562
1103	100	0.1960083187808337
1104	1	0.24399108564227845
1104	2	0.24594434582449054
1104	3	0.2610673977555962
1104	4	0.2148146632259511
1104	5	0.18551661148607224
1104	6	0.22261707742976
1104	7	0.20965571909673741
1104	8	0.23718371545562889
1104	9	0.2519064405717444
1104	10-11	0.24946573634198188
1104	12-13	0.2772662889981845
1104	14-15	0.3852273825453665
1104	16-17	0.4713081835187367
1104	18-19	0.3627001520119748
1104	20-21	0.3628027085986929
1104	22-23	0.3940848216989039
1104	24-25	0.3953053517854741
1104	26-27	0.3885743446772736
1104	28-29	0.37857029311478385
1104	30-31	0.373100450015329
1104	32-33	0.407725469902104
1104	34-35	0.374896907867587
1104	36-37	0.39598322297366906
1104	38-39	0.3508014849859791
1104	40-41	0.39160662727537243
1104	42-43	0.3882038169266906
1104	44-45	0.41644435672203883
1104	46-47	0.46987774335647003
1104	48-49	0.501573181205913
1104	50-51	0.5014967227272678
1104	52-53	0.4546855291201055
1104	54-55	0.41981470569358237
1104	56-57	0.38263890078144414
1104	58-59	0.41973477622256894
1104	60-61	0.44490298683529517
1104	62-63	0.4207368176499884
1104	64-65	0.43248677135725444
1104	66-67	0.39163735604180516
1104	68-69	0.3989084183542033
1104	70-71	0.4168265795955648
1104	72-73	0.3840208635905711
1104	74-75	0.30592830916525315
1104	76-77	0.36390755922117535
1104	78-79	0.3366966450420037
1104	80-81	0.28320052712663824
1104	82-83	0.30658661972519496
1104	84-85	0.2918909580535214
1104	86-87	0.32307124819779176
1104	88-89	0.3151348238843781
1104	90-91	0.28981712608104715
1104	92-93	0.29542375044434976
1104	94-95	0.30612725614438574
1104	96-97	0.2480839113869493
1104	98-99	0.25915823099836643
1104	100	0.2437815650544053
1105	1	0.16833871725758343
1105	2	0.19470078871605523
1105	3	0.15823003729791907
1105	4	0.11027814745740017
1105	5	0.09223428462158978
1105	6	0.12195583945785415
1105	7	0.14055244314543813
1105	8	0.11714494687047505
1105	9	0.16443916729947006
1105	10-11	0.14802416496726778
1105	12-13	0.18334043406364486
1105	14-15	0.27542897684634937
1105	16-17	0.3322298653563962
1105	18-19	0.23380220261369544
1105	20-21	0.2367557021282849
1105	22-23	0.26224436478928936
1105	24-25	0.23240882561931642
1105	26-27	0.2260208783372022
1105	28-29	0.25061573994292985
1105	30-31	0.28098257115222225
1105	32-33	0.23417108089078909
1105	34-35	0.24037615244806432
1105	36-37	0.12397038578299657
1105	38-39	0.1206449762896824
1105	40-41	0.12275929599658042
1105	42-43	0.13713937864379488
1105	44-45	0.17959166095297974
1105	46-47	0.1512785117027562
1105	48-49	0.19379689502795117
1105	50-51	0.12690696309015692
1105	52-53	0.08213483781921838
1105	54-55	0.07912354237842578
1105	56-57	0.04989856131336268
1105	58-59	0.05063169054294292
1105	60-61	0.035194491819822815
1105	62-63	-0.004608598617593884
1105	64-65	0.05133713234652504
1105	66-67	-0.0048978992500892105
1105	68-69	0.02932429661311886
1105	70-71	0.047725777176019335
1105	72-73	0.04399745139304656
1105	74-75	0.028560246470448192
1105	76-77	-0.014709754095967753
1105	78-79	0.003428155319014081
1105	80-81	-0.01773900512118587
1105	82-83	-0.007354263744232981
1105	84-85	-0.02667210734607295
1105	86-87	0.009559602985966364
1105	88-89	0.11346011116533816
1105	90-91	0.017218676590275095
1105	92-93	0.04309432753541387
1105	94-95	0.045891474414681
1105	96-97	-0.006967684436197885
1105	98-99	0.04548587940441706
1105	100	0.044492654204589144
1106	1	0.14758611826396617
1106	2	0.11440080365343874
1106	3	0.13596483293441253
1106	4	0.1314807909287623
1106	5	0.1311463691894872
1106	6	0.14814405831953792
1106	7	0.11611588355975755
1106	8	0.10424289153080935
1106	9	0.14676163535687436
1106	10-11	0.15930850980684852
1106	12-13	0.19322694894864156
1106	14-15	0.23355519329575714
1106	16-17	0.2655256044114225
1106	18-19	0.19145631686017595
1106	20-21	0.18240353041077384
1106	22-23	0.19626237938779667
1106	24-25	0.1646699277754209
1106	26-27	0.15899550540419938
1106	28-29	0.1609244728513204
1106	30-31	0.2051177773438866
1106	32-33	0.21482556411298503
1106	34-35	0.22096538214714911
1106	36-37	0.17738214317755308
1106	38-39	0.22513430163262882
1106	40-41	0.17372229754160173
1106	42-43	0.17437853176091522
1106	44-45	0.12740249791475833
1106	46-47	0.19707572777538473
1106	48-49	0.29904206322959936
1106	50-51	0.20759527837600444
1106	52-53	0.1712585957310111
1106	54-55	0.2241574608336805
1106	56-57	0.18767339806111494
1106	58-59	0.18471764795133083
1106	60-61	0.22219521806659515
1106	62-63	0.22207977184740457
1106	64-65	0.20613036102898263
1106	66-67	0.19728957775860323
1106	68-69	0.18465720802104357
1106	70-71	0.17878830741307183
1106	72-73	0.20873020038477108
1106	74-75	0.1984850746347746
1106	76-77	0.1252778640375709
1106	78-79	0.12988428566665533
1106	80-81	0.15580621614162737
1106	82-83	0.12591185135389082
1106	84-85	0.12578984412303384
1106	86-87	0.13360231443358117
1106	88-89	0.15128550093076853
1106	90-91	0.07153310075405983
1106	92-93	0.10799935809016858
1106	94-95	0.14400268938149452
1106	96-97	0.13191190574436717
1106	98-99	0.1701534529041595
1106	100	0.261956575959438
1107	1	0.1396765070149968
1107	2	0.09366326850042128
1107	3	0.0842832448450821
1107	4	0.09213998034149995
1107	5	0.111758974648545
1107	6	0.13499017908049638
1107	7	0.12843535419664676
1107	8	0.15238343743651228
1107	9	0.11189420773073522
1107	10-11	0.1632904452907198
1107	12-13	0.13623935589653513
1107	14-15	0.245332265256053
1107	16-17	0.27406787074723127
1107	18-19	0.19267193307192088
1107	20-21	0.16898397706089696
1107	22-23	0.17823425697257989
1107	24-25	0.18297728923117518
1107	26-27	0.15587060879543202
1107	28-29	0.19118207417473343
1107	30-31	0.18787542829508652
1107	32-33	0.14395191647031425
1107	34-35	0.10217113897097363
1107	36-37	0.10493105963743687
1107	38-39	0.1289955423274236
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2216	40-41	-0.48228832640354113
2216	42-43	-0.4609351074164678
2216	44-45	-0.49551997761965083
2216	46-47	-0.49188689442026856
2216	48-49	-0.4247335994001844
2216	50-51	-0.4695510266109366
2216	52-53	-0.4840349030988378
2216	54-55	-0.3792631796717032
2216	56-57	-0.42691129104211
2216	58-59	-0.4907110398750518
2216	60-61	-0.5355720427579627
2216	62-63	-0.5507475132090534
2216	64-65	-0.4384414008634394
2216	66-67	-0.48761680137021557
2216	68-69	-0.45736632373527186
2216	70-71	-0.47288738008350606
2216	72-73	-0.47468390597107657
2216	74-75	-0.3905193890446981
2216	76-77	-0.43600782908490743
2216	78-79	-0.3995249866530699
2216	80-81	-0.4154116928952547
2216	82-83	-0.4034599884897858
2216	84-85	-0.3476362723700106
2216	86-87	-0.32770127926048076
2216	88-89	-0.259211067979372
2216	90-91	-0.29657271014183095
2216	92-93	-0.3038838878566281
2216	94-95	-0.25045990547830854
2216	96-97	-0.29826440165872015
2216	98-99	-0.34461826630906245
2216	100	-0.40554154139423915
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	25.0
8	144.0
9	279.0
10	256.0
11	199.0
12	186.0
13	288.0
14	560.0
15	1050.0
16	1859.0
17	3373.0
18	5579.0
19	8791.0
20	12456.0
21	18282.0
22	25840.0
23	29778.0
24	28041.0
25	24355.0
26	23897.0
27	27262.0
28	33958.0
29	41645.0
30	51862.0
31	65898.0
32	84272.0
33	110563.0
34	158097.0
35	238402.0
36	419616.0
37	965733.0
38	1447509.0
39	530011.0
40	1715.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.589574423304164	20.474599338141598	13.701165932771437	24.2346603057828
2	14.801381138200945	21.143588850014446	40.85227507210624	23.20275493967838
3	17.45304939628797	27.075667762935417	30.993003978893864	24.478278861882746
4	11.862994757845684	18.43908398319318	40.0601377447497	29.637783514211435
5	12.284082475616604	38.731387033015054	35.980358013672266	13.00417247769608
6	28.54735794679439	39.72248525187032	18.655530004104026	13.074626797231273
7	25.116162411638733	33.62935919983144	23.947694760465964	17.306783628063858
8	24.698104071559975	35.89466473228287	21.271878742748353	18.1353524534088
9	25.957075302777906	15.176402833890215	21.440728987124057	37.42579287620782
10-11	23.92949618252006	26.73620711553866	29.410506449434155	19.92379025250712
12-13	24.857850497308323	24.66631176576724	29.11491888290586	21.360918854018575
14-15	22.1458122768471	25.657647297393186	27.271541351414026	24.92499907434569
16-17	21.20766409488814	28.498859693919954	27.8742849085835	22.419191302608404
18-19	20.966493923576085	27.685901339270973	30.2883848230629	21.05921991409004
20-21	22.156788818488085	26.77305539733111	29.977533210990075	21.092622573190734
22-23	21.960307039716113	26.76818253827966	29.683837863478246	21.587672558525977
24-25	21.55833821092806	27.003453405844997	30.00053189282085	21.437676490406098
26-27	21.566508550427923	27.169062100936088	29.63372667518003	21.63070267345596
28-29	21.271861196149004	27.352106857267707	29.851601902984125	21.524430043599164
30-31	21.88229419550261	26.974707303658395	29.72769330270053	21.415305198138462
32-33	21.799575103538853	27.16171204409404	29.3719393865126	21.6667734658545
34-35	21.86727485964138	27.161291158286875	29.678936790275788	21.29249719179595
36-37	21.86117823457396	26.97382719898757	29.574680520338028	21.590314046100445
38-39	21.784527057319547	27.087261659570316	29.597114644020706	21.53109663908943
40-41	21.84706508786882	26.7603941759715	29.7308023199364	21.66173841622328
42-43	21.86447462630517	26.835689366339118	29.33877239595477	21.961063611400938
44-45	21.891641806648515	27.226156162793153	29.121172757027214	21.76102927353112
46-47	22.009885411486728	27.097577801361417	28.720916983223137	22.171619803928717
48-49	22.252436706724104	27.09705456157391	28.581827344052428	22.068681387649555
50-51	22.155766188169466	27.297656622375126	28.254112253687197	22.292464935768212
52-53	22.032758975062052	27.423556487950467	28.24519606906972	22.298488467917757
54-55	22.0019242194995	27.411690923073976	28.18411843728066	22.402266420145864
56-57	22.134180877906054	27.382558459838553	28.089460796535544	22.393799865719853
58-59	21.896701794382782	27.348939376518672	28.40780324321623	22.346555585882317
60-61	22.026247447273263	27.52395106788949	28.22151391956109	22.228287565276155
62-63	22.01716044500232	27.305833061081785	28.098251285313893	22.578755208602004
64-65	21.689629099432754	27.209714789573567	28.289381676487622	22.811274434506053
66-67	21.894034513587094	27.215120875734783	28.13938684671443	22.751457763963693
68-69	21.78209513581898	27.084193334685263	28.31285130613404	22.82086022336172
70-71	21.832983654471583	26.803612693537566	28.327782262603744	23.035621389387114
72-73	21.89020284328635	26.98143795812725	28.25167029947201	22.876688899114395
74-75	21.821227153355593	27.168258165720488	28.109894309820092	22.900620371103823
76-77	21.697239045536037	27.08581355489782	27.997533926565076	23.21941347300107
78-79	21.70052688949948	27.079630435672264	27.96888757799227	23.250955096835995
80-81	21.59143028208643	27.22774825267173	28.10840788994185	23.07241357529999
82-83	21.834312126361223	26.82946372780005	28.38606389477433	22.950160251064393
84-85	21.961444462785664	26.93902232126481	27.850374619419682	23.249158596529842
86-87	21.358133294633546	27.21372301818913	28.131341303013606	23.29680238416372
88-89	21.66946254293831	26.82545272217931	27.98825754892325	23.51682718595913
90-91	21.598746016822027	26.821234261876054	28.019792832331564	23.560226888970355
92-93	21.73264723673943	26.7913085413707	27.914623777719928	23.56142044416994
94-95	21.228691461521944	26.743758308680736	28.143496970329878	23.88405325946744
96-97	21.341058496179354	26.664455787206254	28.049778489824128	23.944707226790264
98-99	21.099269357755173	26.84209814389954	27.959543571556466	24.099088926788816
100	21.349323617407904	26.642063288118795	27.724390154365743	24.28422294010756
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	126.0
1	83.5
2	133.0
3	219.5
4	151.0
5	129.0
6	284.0
7	760.5
8	1427.5
9	1793.0
10	1555.5
11	1270.0
12	1381.5
13	1658.0
14	2060.0
15	2625.5
16	3097.5
17	3498.0
18	4126.0
19	4990.5
20	5927.0
21	7067.0
22	8552.5
23	10463.5
24	12967.5
25	17437.0
26	24203.5
27	31153.5
28	37353.0
29	45147.0
30	54673.0
31	64441.5
32	76788.5
33	88782.0
34	99039.0
35	107959.0
36	118201.0
37	132263.5
38	140827.0
39	142181.5
40	142455.0
41	143447.5
42	146347.5
43	152402.5
44	160247.0
45	170235.5
46	177951.5
47	184312.0
48	188772.0
49	183807.0
50	173507.5
51	166424.5
52	159320.5
53	149356.5
54	138810.0
55	123854.0
56	108708.0
57	92487.5
58	76964.5
59	66468.0
60	53540.5
61	40298.0
62	30785.0
63	23294.0
64	16709.5
65	10926.0
66	7314.5
67	5164.5
68	3585.5
69	2493.0
70	1786.0
71	1103.5
72	696.5
73	519.0
74	353.5
75	254.0
76	155.5
77	82.0
78	42.5
79	21.5
80	15.0
81	9.0
82	6.0
83	5.0
84	4.0
85	1.5
86	1.5
87	1.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0028199490070684426
2	0.0036911527653497507
3	3.668226350658137E-4
4	0.04867277839029516
5	0.002384347127927789
6	0.004837473499930418
7	0.0
8	2.980433909909736E-4
9	6.877924407484008E-5
10-11	6.877924407484008E-5
12-13	0.0
14-15	3.438962203742004E-5
16-17	3.095065983367803E-4
18-19	1.146320734580668E-4
20-21	2.178009395703269E-4
22-23	0.0
24-25	0.0
26-27	2.521905616077469E-4
28-29	0.0
30-31	1.8341131753290684E-4
32-33	2.86580183645167E-4
34-35	1.719481101871002E-4
36-37	2.2926414691613355E-5
38-39	7.221820627858207E-4
40-41	5.387707452529139E-4
42-43	0.0
44-45	5.73160367290334E-4
46-47	0.0
48-49	5.73160367290334E-5
50-51	0.0
52-53	3.3243301302839366E-4
54-55	2.86580183645167E-4
56-57	1.031688661122601E-4
58-59	6.992556480942074E-4
60-61	5.387707452529139E-4
62-63	1.3755848814968016E-4
64-65	4.356018791406538E-4
66-67	0.0011348575272348612
68-69	0.0025562952381148894
70-71	0.0036682263506581376
72-73	0.003163845227442643
74-75	0.0026938537262645693
76-77	0.004390408413443958
78-79	0.0011577839419264747
80-81	0.0011577839419264747
82-83	7.221820627858207E-4
84-85	4.814547085238805E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	1.031688661122601E-4
94-95	5.73160367290334E-5
96-97	4.012122571032337E-4
98-99	4.585282938322671E-5
100	0.07568009489701569
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4361781.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.05895272713019
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.68017211316129	33.37939583649646
2	16.25270041029946	16.271863230547805
3	6.86069348083128	10.303173918967898
4	3.5004094490986035	7.009073245521074
5	2.0045960189914642	5.017398867584354
6	1.2787287967466265	3.840709463229567
7	0.8197906864572381	2.872650425365314
8	0.5856413563143995	2.345327437663595
9	0.412863744615989	1.860077400702997
>10	1.5429746633298425	12.144110722845342
>50	0.03848475466241322	1.3049789338599116
>100	0.021182568879845886	1.9164390269528704
>500	0.001174637741100585	0.43034030770006243
>1k	3.3561078317122597E-4	0.22180628196000646
>5k	1.6780539158561299E-4	0.6324062085725842
>10k+	8.390269579280649E-5	0.45024869203015894
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10720	0.24577116549409517	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9752	0.22357839607261343	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5305	0.12162462993900885	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	2.2926414691613358E-5
7	0.0	0.0	0.0	0.0	2.2926414691613358E-5
8	0.0	0.0	0.0	0.0	2.2926414691613358E-5
9	0.0	0.0	0.0	0.0	6.877924407484006E-5
10-11	1.1463207345806679E-5	0.0	0.0	0.0	6.877924407484006E-5
12-13	1.1463207345806679E-4	0.0	0.0	0.0	6.877924407484006E-5
14-15	1.1463207345806679E-4	0.0	0.0	0.0	1.7194811018710017E-4
16-17	1.1463207345806679E-4	0.0	0.0	1.1463207345806679E-5	2.8658018364516693E-4
18-19	1.3755848814968013E-4	0.0	0.0	2.2926414691613358E-5	3.20969805682587E-4
20-21	1.3755848814968013E-4	0.0	0.0	3.438962203742004E-5	3.3243301302839366E-4
22-23	1.3755848814968013E-4	0.0	0.0	1.1463207345806679E-4	3.782858424116204E-4
24-25	1.3755848814968013E-4	0.0	0.0	3.668226350658137E-4	4.126754644490404E-4
26-27	1.3755848814968013E-4	0.0	0.0	8.826669656271142E-4	4.241386717948471E-4
28-29	1.3755848814968013E-4	0.0	0.0	0.0029116546658348964	4.356018791406538E-4
30-31	1.3755848814968013E-4	0.0	0.0	0.010293960196534398	4.356018791406538E-4
32-33	1.3755848814968013E-4	0.0	0.0	0.031076755114481904	5.502339525987206E-4
34-35	1.3755848814968013E-4	0.0	0.0	0.06217643664365542	6.190131966735606E-4
36-37	1.3755848814968013E-4	0.0	0.0	0.11233943198890545	6.190131966735606E-4
38-39	1.604849028412935E-4	0.0	0.0	0.18922316365723085	6.190131966735606E-4
40-41	2.178009395703269E-4	0.0	0.0	0.27752424984197965	6.41939611365174E-4
42-43	2.2926414691613358E-4	0.0	0.0	0.3722332689330345	6.41939611365174E-4
44-45	2.2926414691613358E-4	0.0	0.0	0.48803458954037354	6.534028187109807E-4
46-47	2.2926414691613358E-4	0.0	0.0	0.6245155361995478	6.877924407484007E-4
48-49	2.2926414691613358E-4	0.0	0.0	0.7798419957352283	7.107188554400141E-4
50-51	2.521905616077469E-4	0.0	0.0	0.9509647549934304	7.107188554400141E-4
52-53	2.521905616077469E-4	0.0	0.0	1.1220072718002119	7.565716848232408E-4
54-55	2.521905616077469E-4	0.0	0.0	1.3088690147442066	8.138877215522742E-4
56-57	2.521905616077469E-4	0.0	0.0	1.5308769514104446	8.253509288980808E-4
58-59	3.20969805682587E-4	0.0	0.0	1.7885010733001039	8.253509288980808E-4
60-61	3.3243301302839366E-4	0.0	0.0	2.058929597795029	8.482773435896942E-4
62-63	3.5535942772000706E-4	0.0	0.0	2.337153103285103	9.170565876645343E-4
64-65	3.6682263506581373E-4	0.0	0.0	2.627103469889937	9.170565876645343E-4
66-67	3.8974904975742707E-4	0.0	0.0	2.9574845688034315	9.28519795010341E-4
68-69	3.8974904975742707E-4	0.0	0.0	3.328101525500707	9.399830023561476E-4
70-71	3.8974904975742707E-4	0.0	0.0	3.7331195674427486	9.62909417047761E-4
72-73	3.8974904975742707E-4	0.0	0.0	4.147525976201006	0.001146320734580668
74-75	4.0121225710323374E-4	0.0	0.0	4.563147484937918	0.001146320734580668
76-77	4.356018791406538E-4	0.0	0.0	5.016345387354386	0.0011577839419264747
78-79	4.356018791406538E-4	0.0	0.0	5.516909262523726	0.0011692471492722812
80-81	4.356018791406538E-4	0.0	0.0	6.060632113350028	0.001215099978655508
82-83	4.356018791406538E-4	0.0	0.0	6.621022467657133	0.001283879222730348
84-85	4.5852829383226715E-4	0.0	0.0	7.174122222092306	0.001283879222730348
86-87	5.502339525987205E-4	0.0	0.0	7.749781568584026	0.001283879222730348
88	5.502339525987205E-4	0.0	0.0	8.205799420007562	0.0013068056374219614
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7160	0.0	57.085236	1
GTATCAA	13300	0.0	44.028584	1
ATCAACG	16605	0.0	34.86634	3
CAACGCA	16795	0.0	34.50534	5
TATCAAC	17465	0.0	33.50147	2
AACGCAG	17880	0.0	32.752453	6
TCAACGC	17815	0.0	32.44466	4
ACGCAGA	19635	0.0	29.464937	7
CGCAGAG	19955	0.0	28.898226	8
GCAGAGT	22565	0.0	25.159674	9
GTACATG	13520	0.0	22.925867	1
TACATGG	13300	0.0	22.420725	2
GAGTACT	15145	0.0	22.296457	12-13
AGAGTAC	20465	0.0	22.161234	10-11
ACATGGG	13015	0.0	21.970833	3
CAGAGTA	21850	0.0	21.896498	10-11
GTGGTAT	2645	0.0	20.094158	1
GTACTTT	16585	0.0	20.07718	14-15
AGTACTT	16590	0.0	18.90964	12-13
CTAGTAC	2690	0.0	18.707624	3
>>END_MODULE